Restriction map for
pFN21AB1292
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# NO CUT ENZYMES ( 95 enzymes) #
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AatII AccI AccIII AclI AflII
AflIII AgeI AlwNI AscI AseI
AsnI AspI AviII AvrII BbrPI
BcgI BciVI BclI BfrI BglI
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaI BsaWI BseAI BsiWI
BsmI BspDI BspEI BspHI BspLU11I
BssHII Bst1107I BstAPI BstBI BstXI
BstZ17I CelII ClaI CpoI DrdI
EagI EclXI Eco52I EcoRI EcoRV
FbaI FseI FspI HincII HindII
HindIII HpaI KspI MamI MfeI
MluI MroI MunI NaeI NgoMIV
NheI NotI NruI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I RcaI RsrII
SacI SacII SalI ScaI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SwaI Tth111I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 391 GGTACC G^GTACC
AcsI 497 GAATTT [GA]^AATT[CT]
AhdI 1255 GACACCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 321 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1909 AGCGCT AGC^GCT
ApaI 1095 GGGCCC GGGCC^C
ApoI 497 GAATTT [GA]^AATT[CT]
Asp718I 391 GGTACC G^GTACC
AspEI 1255 GACACCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 2 TGGCCA TGG^CCA
BbsI 517 GAAGAC GAAGAC(2/6)
BbvCI 1730 CCTCAGC CCTCAGC(-5/-2)
BcnI 540 CCGGG CC^[GC]GG
BfaI 184 CTAG C^TAG
BglII 1065 AGATCT A^GATCT
BpuAI 517 GAAGAC GAAGAC(2/6)
BsiEI 1718 CGATCG CG[GA][CT]^CG
BsmBI 244 GAGACG CGTCTC(1/5)
BsrDI 1511 GCAATG GCAATG(2/0)
BssSI 758 CACGAG CACGAG(-5/-1)
BstEII 123 GGTGACC G^GT[ACGT]ACC
BstPI 123 GGTGACC G^GT[ACGT]ACC
Bsu36I 321 CCTGAGG CC^T[ACGT]AGG
DraI 811 TTTAAA TTT^AAA
DraIII 1033 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1255 GACACCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1909 AGCGCT AGC^GCT
Eco81I 321 CCTGAGG CC^T[ACGT]AGG
EcoO65I 123 GGTGACC G^GT[ACGT]ACC
EcoT22I 141 ATGCAT ATGCA^T
KpnI 391 GGTACC GGTAC^C
MaeI 184 CTAG C^TAG
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
NciI 540 CCGGG CC^[GC]GG
NdeI 1949 CATATG CA^TATG
NsiI 141 ATGCAT ATGCA^T
NspI 139 GCATGC [GA]CATG^[CT]
PaeR7I 1299 CTCGAG C^TCGAG
PflMI 1668 CCAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuI 1718 CGATCG CGAT^CG
PvuII 304 CAGCTG CAG^CTG
SbfI 1467 CCTGCAGG CCTGCA^GG
SexAI 822 ACCAGGT A^CC[AT]GGT
SfaNI 711 GATGC GCATC(5/9)
SphI 139 GCATGC GCATG^C
Sse8387I 1467 CCTGCAGG CCTGCA^GG
SspI 156 AATATT AAT^ATT
StuI 1638 AGGCCT AGG^CCT
Van91I 1668 CCAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1529 CCATCGGTCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1299 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 1314 CGCG CG^CG
1397 CGCG
Alw44I 1039 GTGCAC G^TGCAC
1192 GTGCAC
ApaLI 1039 GTGCAC G^TGCAC
1192 GTGCAC
Asp700I 202 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1860 GAACATCTTC
BamHI 1820 GGATCC G^GATCC
1940 GGATCC
BbeI 187 GGCGCC GGCGC^C
1847 GGCGCC
Bsp1407I 236 TGTACA T^GTACA
596 TGTACA
BspMI 1159 ACCTGC ACCTGC(4/8)
1470 GCAGGT
BsrBI 856 CCGCTC CCGCTC(-3/-3)
1824 CCGCTC
BsrFI 169 ACCGGC [GA]^CCGG[CT]
1130 ACCGGC
BsrGI 236 TGTACA T^GTACA
596 TGTACA
BstUI 1314 CGCG CG^CG
1397 CGCG
Cfr10I 169 ACCGGC [GA]^CCGG[CT]
1130 ACCGGC
EcoNI 317 CCTTCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
777 CCTCAGTAAGG
HgaI 1435 GACGC GACGC(5/10)
1369 GCGTC
KasI 187 GGCGCC G^GCGCC
1847 GGCGCC
MaeII 227 ACGT A^CGT
1517 ACGT
MseI 508 TTAA T^TAA
812 TTAA
MvnI 1314 CGCG CG^CG
1397 CGCG
NarI 187 GGCGCC GG^CGCC
1847 GGCGCC
PstI 1409 CTGCAG CTGCA^G
1468 CTGCAG
SfoI 187 GGCGCC GGC^GCC
1847 GGCGCC
SmlI 129 CTTGAG C^T[CT][GA]AG
1299 CTCGAG
SspBI 236 TGTACA T^GTACA
596 TGTACA
TfiI 371 GATTC G^A[AT]TC
1628 GAATC
Tru9I 508 TTAA T^TAA
812 TTAA
Tsp45I 124 GTGAC ^GT[GC]AC
1882 GTGAC
Tsp509I 498 AATT ^AATT
734 AATT
XmnI 202 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1860 GAACATCTTC
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# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AspHI AvaI AvaII BanI BanII
BbvI BmyI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MwoI NcoI
NdeII NlaIII NlaIV PleI PpuMI
RsaI SapI Sau3AI Sau96I ScrFI
SfcI StyI TaqI TseI TspRI
TthHB8I XhoII