(Circular) (Six-base) MAPSORT of: pFN21AB1292.seq Check: 1873 from: 1 to: 6661 pFN21AB1292 6661 bp With 182 enzymes: SgfI * February 27, 2009 14:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3173 3173 Size: 6661 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4471 278 Size: 53 83 186 936 2935 2468 Fragments arranged by size: 2935 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4004 1937 Size: 2067 4594 Acc65I G'GTAC_C Cuts at: 2396 3982 2396 Size: 1586 5075 AclI AA'CG_TT Cuts at: 1563 4788 5161 1563 Size: 3225 373 3063 Fragments arranged by size: 3225 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4724 6108 6376 1632 Size: 3092 1384 268 1917 Fragments arranged by size: 3092 1917 1384 268 AfeI AGC'GCT Cuts at: 3916 3916 Size: 6661 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6464 AflIII A'CryG_T Cuts at: 1203 5560 6537 1203 Size: 4357 977 1327 Fragments arranged by size: 4357 1327 977 AgeI A'CCGG_T Cuts at: 5492 5492 Size: 6661 AhdI GACnn_n'nnGTC Cuts at: 3265 5390 3265 Size: 2125 4536 AleI CACnn'nnGTG Cuts at: 1166 3749 1166 Size: 2583 4078 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2331 1184 Size: 1147 5514 AlwNI CAG_nnn'CTG Cuts at: 4404 5976 4404 Size: 1572 5089 ApaI G_GGCC'C Cuts at: 3104 3104 Size: 6661 ApaLI G'TGCA_C Cuts at: 3044 3197 4718 5874 3044 Size: 153 1521 1156 3831 Fragments arranged by size: 3831 1521 1156 153 ApoI r'AATT_y Cuts at: 1493 2502 3970 4196 4348 6457 1493 Size: 1009 1468 226 152 2109 1697 Fragments arranged by size: 2109 1697 1468 1009 226 152 AseI AT'TA_AT Cuts at: 160 1032 5215 160 Size: 872 4183 1606 Fragments arranged by size: 4183 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6661 AvaI C'yCGr_G Cuts at: 1870 1943 2925 3055 3104 3304 3446 3986 1870 Size: 73 982 130 49 200 142 540 4545 Fragments arranged by size: 4545 982 540 200 142 130 73 49 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2097 2130 2097 Size: 33 6628 BamHI G'GATC_C Cuts at: 3825 3945 3991 4358 3825 Size: 120 46 367 6128 Fragments arranged by size: 6128 367 120 46 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2192 2396 2831 2990 3852 Size: 325 217 627 405 204 435 159 862 Cuts at: 3852 3982 5437 618 Size: 130 1455 1842 Fragments arranged by size: 1842 1455 862 627 435 405 325 217 204 159 130 BanII G_rGCy'C Cuts at: 1391 2120 3063 3104 3806 3980 1391 Size: 729 943 41 702 174 4072 Fragments arranged by size: 4072 943 729 702 174 41 BbeI G_GCGC'C Cuts at: 2196 3856 2196 Size: 1660 5001 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2529 928 Size: 971 630 5060 Fragments arranged by size: 5060 971 630 BbvCI CC'TCA_GC Cuts at: 3736 3736 Size: 6661 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4852 4886 1903 Size: 34 2915 34 3678 Fragments arranged by size: 3678 2915 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4553 5763 4553 Size: 1210 5451 BclI T'GATC_A Cuts at: 1548 1548 Size: 6661 BfrBI ATG'CAT Cuts at: 2148 2148 Size: 6661 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5272 136 Size: 107 122 71 631 605 3600 1525 Fragments arranged by size: 3600 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3070 6656 3070 Size: 3586 3075 BlpI GC'TnA_GC Cuts at: 4078 4078 Size: 6661 Bme1580I G_kGCm'C Cuts at: 1790 2551 3048 3104 3201 4722 5878 1790 Size: 761 497 56 97 1521 1156 2573 Fragments arranged by size: 2573 1521 1156 761 497 97 56 BmgBI CAC'GTC Cuts at: 1318 2233 3523 1318 Size: 915 1290 4456 Fragments arranged by size: 4456 1290 915 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2549 2804 2897 Size: 416 53 83 399 24 1132 255 93 Cuts at: 2897 4392 5350 442 Size: 1495 958 1753 Fragments arranged by size: 1753 1495 1132 958 416 399 255 93 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6661 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3751 3751 Size: 6661 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5321 1239 Size: 4082 2579 Bpu10I CC'TnA_GC Cuts at: 3736 3736 Size: 6661 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2155 4786 5651 5949 2155 Size: 2631 865 298 2867 Fragments arranged by size: 2867 2631 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5324 882 Size: 617 3825 2219 Fragments arranged by size: 3825 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4452 493 Size: 711 3248 2702 Fragments arranged by size: 3248 2702 711 BsaBI GATnn'nnATC Cuts at: 6655 6655 Size: 6661 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2193 3374 3853 4468 Size: 53 83 186 936 660 1181 479 615 Cuts at: 4468 4850 5485 275 Size: 382 635 1451 Fragments arranged by size: 1451 1181 936 660 635 615 479 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5094 5492 5766 5913 6324 5094 Size: 398 274 147 411 5431 Fragments arranged by size: 5431 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2601 2631 3010 3040 1178 Size: 30 165 30 1198 30 379 30 4799 Fragments arranged by size: 4799 1198 379 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3122 3362 3434 3437 3446 3639 1186 Size: 1936 240 72 3 9 193 4208 Fragments arranged by size: 4208 1936 240 193 72 9 3 BseYI C'CCAG_C Cuts at: 2270 3902 5864 6360 2270 Size: 1632 1962 496 2571 Fragments arranged by size: 2571 1962 1632 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2429 3062 3622 1163 Size: 573 693 633 560 4202 Fragments arranged by size: 4202 693 633 573 560 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3726 4111 4872 5900 1540 Size: 275 186 1725 385 761 1028 2301 Fragments arranged by size: 2301 1725 1028 761 385 275 186 BsiHKAI G_wGCw'C Cuts at: 2614 2622 2947 3048 3201 4722 4807 5878 2614 Size: 8 325 101 153 1521 85 1071 3397 Fragments arranged by size: 3397 1521 1071 325 153 101 85 8 BsmI GAATG_Cn' Cuts at: 4172 4265 4172 Size: 93 6568 BsmBI CGTCTCn'nnnn_ Cuts at: 2243 2243 Size: 6661 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2120 2551 2614 2622 2947 3048 3063 Size: 399 330 431 63 8 325 101 15 Cuts at: 3063 3104 3201 3806 3980 4722 4807 5878 1391 Size: 41 97 605 174 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 605 431 399 330 325 174 101 97 85 63 41 15 8 BspEI T'CCGG_A Cuts at: 6324 6324 Size: 6661 BspHI T'CATG_A Cuts at: 4550 6280 4550 Size: 1730 4931 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3173 3466 4016 844 Size: 2329 293 550 3489 Fragments arranged by size: 3489 2329 550 293 BsrBI CCG'CTC Cuts at: 1394 1823 2863 3831 4548 1394 Size: 429 1040 968 717 3507 Fragments arranged by size: 3507 1040 968 717 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3523 5156 5330 65 Size: 1183 2275 1633 174 1396 Fragments arranged by size: 2275 1633 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2174 3135 5305 5492 1700 Size: 110 364 961 2170 187 2869 Fragments arranged by size: 2869 2170 961 364 187 110 BsrGI T'GTAC_A Cuts at: 96 2241 2601 96 Size: 2145 360 4156 Fragments arranged by size: 4156 2145 360 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6661 BssSI C'ACGA_G Cuts at: 2763 4721 5733 2763 Size: 1958 1012 3691 Fragments arranged by size: 3691 1958 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6661 BstBI TT'CG_AA Cuts at: 5465 5465 Size: 6661 BstEII G'GTnAC_C Cuts at: 1184 2128 1184 Size: 944 5717 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 6661 BstYI r'GATC_y Cuts at: 1972 3070 3825 3945 3991 4358 4743 4760 6212 Size: 1098 755 120 46 367 385 17 1452 Cuts at: 6212 6298 6310 6656 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1098 755 385 367 346 120 86 46 17 12 Bsu36I CC'TnA_GG Cuts at: 2327 2327 Size: 6661 BtgI C'CryG_G Cuts at: 513 1065 2004 2010 2112 2286 2711 2959 3678 Size: 552 939 6 102 174 425 248 719 Cuts at: 3678 3816 513 Size: 138 3358 Fragments arranged by size: 3358 939 719 552 425 248 174 138 102 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5922 Fragments arranged by size: 5922 683 56 BtsI GCAGTG_nn' Cuts at: 2350 2417 4177 4971 4991 2350 Size: 67 1760 794 20 4020 Fragments arranged by size: 4020 1760 794 67 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6661 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6626 DraI TTT'AAA Cuts at: 2818 3965 2818 Size: 1147 5514 DraIII CAC_nnn'GTG Cuts at: 3043 3043 Size: 6661 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5668 809 Size: 507 4352 1802 Fragments arranged by size: 4352 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 3007 3384 Size: 54 1425 50 275 18 177 1000 377 Cuts at: 3384 3813 4108 4997 6626 8 Size: 429 295 889 1629 43 Fragments arranged by size: 1629 1425 1000 889 429 377 295 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4108 1537 Size: 275 2296 4090 Fragments arranged by size: 4090 2296 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2705 2935 3275 3658 3936 4591 1353 Size: 1352 230 340 383 278 655 3423 Fragments arranged by size: 3423 1352 655 383 340 278 230 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3347 3359 5248 5622 5768 387 Size: 1064 138 1758 12 1889 374 146 1280 Fragments arranged by size: 1889 1758 1280 1064 374 146 138 12 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 4724 5321 6108 6376 1239 Size: 393 3092 597 787 268 1524 Fragments arranged by size: 3092 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2326 2786 2326 Size: 460 6201 EcoO109I rG'GnC_Cy Cuts at: 2892 3100 3101 3409 3478 3498 3688 2892 Size: 208 1 308 69 20 190 5865 Fragments arranged by size: 5865 308 208 190 69 20 1 EcoRI G'AATT_C Cuts at: 3970 4348 3970 Size: 378 6283 FspI TGC'GCA Cuts at: 5167 5167 Size: 6661 HaeII r_GCGC'y Cuts at: 1678 2196 3856 3918 5808 1678 Size: 518 1660 62 1890 2531 Fragments arranged by size: 2531 1890 1660 518 62 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3010 3042 3251 3283 3287 Size: 32 135 32 1438 32 209 32 4 Cuts at: 3287 3319 3618 3650 5302 5334 5376 5408 1373 Size: 32 299 32 1652 32 42 32 2626 Fragments arranged by size: 2626 1652 1438 299 209 135 42 32 32 32 32 32 32 32 32 4 HincII GTy'rAC Cuts at: 669 1657 1938 4005 4251 669 Size: 988 281 2067 246 3079 Fragments arranged by size: 3079 2067 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6661 HpaI GTT'AAC Cuts at: 4251 4251 Size: 6661 KasI G'GCGC_C Cuts at: 2192 3852 2192 Size: 1660 5001 KpnI G_GTAC'C Cuts at: 2400 3986 2400 Size: 1586 5075 MfeI C'AATT_G Cuts at: 4260 4260 Size: 6661 MluI A'CGCG_T Cuts at: 6537 6537 Size: 6661 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3009 3357 3610 3786 5775 5959 1305 Size: 1704 348 253 176 1989 184 2007 Fragments arranged by size: 2007 1989 1704 348 253 184 176 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 10 Size: 54 1425 343 177 4662 Fragments arranged by size: 4662 1425 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2231 3749 3872 4619 4978 5137 Size: 648 843 222 1518 123 747 359 159 Cuts at: 5137 518 Size: 2042 Fragments arranged by size: 2042 1518 843 747 648 359 222 159 123 MspA1I CmG'CkG Cuts at: 1606 2300 2311 3714 4077 4754 5902 6147 1606 Size: 694 11 1403 363 677 1148 245 2120 Fragments arranged by size: 2120 1403 1148 694 677 363 245 11 NarI GG'CG_CC Cuts at: 2193 3853 2193 Size: 1660 5001 NcoI C'CATG_G Cuts at: 513 1065 2004 2010 2286 2959 3678 513 Size: 552 939 6 276 673 719 3496 Fragments arranged by size: 3496 939 719 673 552 276 6 NdeI CA'TA_TG Cuts at: 387 3955 387 Size: 3568 3093 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6661 NotI GC'GGCC_GC Cuts at: 4108 4108 Size: 6661 NsiI A_TGCA'T Cuts at: 2150 2150 Size: 6661 NspI r_CATG'y Cuts at: 1132 1229 2148 4019 5564 1132 Size: 97 919 1871 1545 2229 Fragments arranged by size: 2229 1871 1545 919 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6661 PciI A'CATG_T Cuts at: 5560 5560 Size: 6661 PflMI CCAn_nnn'nTGG Cuts at: 1108 3679 1108 Size: 2571 4090 PmeI GTTT'AAAC Cuts at: 3965 3965 Size: 6661 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4730 4762 6243 6275 4730 Size: 32 1481 32 5116 Fragments arranged by size: 5116 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2892 3409 3478 3498 3688 2892 Size: 517 69 20 190 5865 Fragments arranged by size: 5865 517 190 69 20 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6661 PsiI TTA'TAA Cuts at: 4231 4231 Size: 6661 PspOMI G'GGCC_C Cuts at: 3100 3100 Size: 6661 PspXI vC'TCGA_Gb Cuts at: 3304 3304 Size: 6661 PstI C_TGCA'G Cuts at: 830 3418 3477 4013 830 Size: 2588 59 536 3478 Fragments arranged by size: 3478 2588 536 59 PvuI CG_AT'CG Cuts at: 2001 3726 2001 Size: 1725 4936 PvuII CAG'CTG Cuts at: 2311 2311 Size: 6661 SalI G'TCGA_C Cuts at: 1936 4003 1936 Size: 2067 4594 SapI GCTCTTCn'nnn_ Cuts at: 2705 2935 3936 2705 Size: 230 1001 5430 Fragments arranged by size: 5430 1001 230 SbfI CC_TGCA'GG Cuts at: 3477 4013 3477 Size: 536 6125 ScaI AGT'ACT Cuts at: 4909 6621 4909 Size: 1712 4949 SexAI A'CCwGG_T Cuts at: 2827 2827 Size: 6661 SfcI C'TryA_G Cuts at: 826 1045 2370 3414 3473 4009 5144 5825 6016 Size: 219 1325 1044 59 536 1135 681 191 Cuts at: 6016 826 Size: 1471 Fragments arranged by size: 1471 1325 1135 1044 681 536 219 191 59 SfoI GGC'GCC Cuts at: 2194 3854 2194 Size: 1660 5001 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6661 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6661 SmaI CCC'GGG Cuts at: 1872 3988 1872 Size: 2116 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2134 3304 4765 5666 5928 820 Size: 197 926 191 1170 1461 901 262 1553 Fragments arranged by size: 1553 1461 1170 926 901 262 197 191 SnaBI TAC'GTA Cuts at: 493 4452 493 Size: 3959 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6661 SphI G_CATG'C Cuts at: 1132 2148 4019 1132 Size: 1016 1871 3774 Fragments arranged by size: 3774 1871 1016 SspI AAT'ATT Cuts at: 5 52 2163 4585 5 Size: 47 2111 2422 2081 Fragments arranged by size: 2422 2111 2081 47 StuI AGG'CCT Cuts at: 1517 3645 1517 Size: 2128 4533 StyI C'CwwG_G Cuts at: 513 1065 2004 2010 2286 2835 2959 3678 4100 Size: 552 939 6 276 549 124 719 422 Cuts at: 4100 513 Size: 3074 Fragments arranged by size: 3074 939 719 552 549 422 276 124 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3527 4688 4705 4858 5043 5488 325 Size: 1255 1947 1161 17 153 185 445 1498 Fragments arranged by size: 1947 1498 1255 1161 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2241 2491 2601 Size: 274 80 33 51 1443 264 250 110 Cuts at: 2601 4907 6619 96 Size: 2306 1712 138 Fragments arranged by size: 2306 1712 1443 274 264 250 138 110 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2601 2633 6621 6653 2601 Size: 32 3988 32 2609 Fragments arranged by size: 3988 2609 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6661 XbaI T'CTAG_A Cuts at: 3997 3997 Size: 6661 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3541 3541 Size: 6661 XhoI C'TCGA_G Cuts at: 1943 3304 1943 Size: 1361 5300 XmaI C'CCGG_G Cuts at: 1870 3986 1870 Size: 2116 4545 XmnI GAAnn'nnTTC Cuts at: 2211 3869 4790 2211 Size: 1658 921 4082 Fragments arranged by size: 4082 1658 921 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4469 276 Size: 53 83 186 936 2935 2468 Fragments arranged by size: 2935 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BsiWI BstZ17I EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI PsrI RsrII SacI SacII SanDI SfiI SrfI SwaI