Restriction map for
pFN21AB1292
(Linear) (Six-base) MAPSORT of: KIBB1292.seq Check: 311 from: 1 to: 1956
KIBB1292.orf
With 182 enzymes: SgfI *
July 18, 2007 13:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1168 1956
Size: 1168 788
Acc65I G'GTAC_C
Cuts at: 0 391 1956
Size: 391 1565
AfeI AGC'GCT
Cuts at: 0 1911 1956
Size: 1911 45
AhdI GACnn_n'nnGTC
Cuts at: 0 1260 1956
Size: 1260 696
AleI CACnn'nnGTG
Cuts at: 0 1744 1956
Size: 1744 212
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 326 1956
Size: 326 1630
ApaI G_GGCC'C
Cuts at: 0 1099 1956
Size: 1099 857
ApaLI G'TGCA_C
Cuts at: 0 1039 1192 1956
Size: 1039 153 764
Fragments arranged by size:
1039 764 153
ApoI r'AATT_y
Cuts at: 0 497 1956
Size: 497 1459
AvaI C'yCGr_G
Cuts at: 0 920 1050 1099 1299 1441 1956
Size: 920 130 49 200 142 515
Fragments arranged by size:
920 515 200 142 130 49
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 92 125 1956
Size: 92 33 1831
Fragments arranged by size:
1831 92 33
BamHI G'GATC_C
Cuts at: 0 1820 1940 1956
Size: 1820 120 16
Fragments arranged by size:
1820 120 16
BanI G'GyrC_C
Cuts at: 0 187 391 826 985 1847 1956
Size: 187 204 435 159 862 109
Fragments arranged by size:
862 435 204 187 159 109
BanII G_rGCy'C
Cuts at: 0 115 1058 1099 1801 1956
Size: 115 943 41 702 155
Fragments arranged by size:
943 702 155 115 41
BbeI G_GCGC'C
Cuts at: 0 191 1851 1956
Size: 191 1660 105
Fragments arranged by size:
1660 191 105
BbsI GAAGACnn'nnnn_
Cuts at: 0 524 1956
Size: 524 1432
BbvCI CC'TCA_GC
Cuts at: 0 1731 1956
Size: 1731 225
BfrBI ATG'CAT
Cuts at: 0 143 1956
Size: 143 1813
BglII A'GATC_T
Cuts at: 0 1065 1956
Size: 1065 891
Bme1580I G_kGCm'C
Cuts at: 0 546 1043 1099 1196 1956
Size: 546 497 56 97 760
Fragments arranged by size:
760 546 497 97 56
BmgBI CAC'GTC
Cuts at: 0 228 1518 1956
Size: 228 1290 438
Fragments arranged by size:
1290 438 228
BmrI ACTGGGnnnn_n'
Cuts at: 0 544 799 892 1956
Size: 544 255 93 1064
Fragments arranged by size:
1064 544 255 93
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1746 1956
Size: 1746 210
Bpu10I CC'TnA_GC
Cuts at: 0 1731 1956
Size: 1731 225
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 150 1956
Size: 150 1806
BsaHI Gr'CG_yC
Cuts at: 0 188 1369 1848 1956
Size: 188 1181 479 108
Fragments arranged by size:
1181 479 188 108
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 596 626 1005 1035 1956
Size: 596 30 379 30 921
Fragments arranged by size:
921 596 379 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1117 1357 1429 1432 1441 1634 1956
Size: 1117 240 72 3 9 193 322
Fragments arranged by size:
1117 322 240 193 72 9 3
BseYI C'CCAG_C
Cuts at: 0 265 1897 1956
Size: 265 1632 59
Fragments arranged by size:
1632 265 59
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 424 1057 1617 1956
Size: 424 633 560 339
Fragments arranged by size:
633 560 424 339
BsiEI CG_ry'CG
Cuts at: 0 1721 1956
Size: 1721 235
BsiHKAI G_wGCw'C
Cuts at: 0 609 617 942 1043 1196 1956
Size: 609 8 325 101 153 760
Fragments arranged by size:
760 609 325 153 101 8
BsmBI CGTCTCn'nnnn_
Cuts at: 0 238 1956
Size: 238 1718
Bsp1286I G_dGCh'C
Cuts at: 0 115 546 609 617 942 1043 1058 1099
Size: 115 431 63 8 325 101 15 41
Cuts at: 1099 1196 1801 1956
Size: 97 605 155
Fragments arranged by size:
605 431 325 155 115 101 97 63
41 15 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1168 1461 1956
Size: 1168 293 495
Fragments arranged by size:
1168 495 293
BsrBI CCG'CTC
Cuts at: 0 858 1826 1956
Size: 858 968 130
Fragments arranged by size:
968 858 130
BsrDI GCAATG_nn'
Cuts at: 0 1518 1956
Size: 1518 438
BsrFI r'CCGG_y
Cuts at: 0 169 1130 1956
Size: 169 961 826
Fragments arranged by size:
961 826 169
BsrGI T'GTAC_A
Cuts at: 0 236 596 1956
Size: 236 360 1360
Fragments arranged by size:
1360 360 236
BssSI C'ACGA_G
Cuts at: 0 758 1956
Size: 758 1198
BstEII G'GTnAC_C
Cuts at: 0 123 1956
Size: 123 1833
BstYI r'GATC_y
Cuts at: 0 1065 1820 1940 1956
Size: 1065 755 120 16
Fragments arranged by size:
1065 755 120 16
Bsu36I CC'TnA_GG
Cuts at: 0 322 1956
Size: 322 1634
BtgI C'CryG_G
Cuts at: 0 5 107 281 706 954 1673 1811 1956
Size: 5 102 174 425 248 719 138 145
Fragments arranged by size:
719 425 248 174 145 138 102 5
BtsI GCAGTG_nn'
Cuts at: 0 345 412 1956
Size: 345 67 1544
Fragments arranged by size:
1544 345 67
DraI TTT'AAA
Cuts at: 0 813 1956
Size: 813 1143
DraIII CAC_nnn'GTG
Cuts at: 0 1038 1956
Size: 1038 918
EaeI y'GGCC_r
Cuts at: 0 2 1002 1379 1808 1956
Size: 2 1000 377 429 148
Fragments arranged by size:
1000 429 377 148 2
EarI CTCTTCn'nnn_
Cuts at: 0 700 930 1270 1653 1931 1956
Size: 700 230 340 383 278 25
Fragments arranged by size:
700 383 340 278 230 25
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1342 1354 1956
Size: 1342 12 602
Fragments arranged by size:
1342 602 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 321 781 1956
Size: 321 460 1175
Fragments arranged by size:
1175 460 321
EcoO109I rG'GnC_Cy
Cuts at: 0 887 1095 1096 1404 1473 1493 1683 1956
Size: 887 208 1 308 69 20 190 273
Fragments arranged by size:
887 308 273 208 190 69 20 1
HaeII r_GCGC'y
Cuts at: 0 191 1851 1913 1956
Size: 191 1660 62 43
Fragments arranged by size:
1660 191 62 43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1005 1037 1246 1278 1282 1314 1613 1645
Size: 1005 32 209 32 4 32 299 32
Cuts at: 1645 1956
Size: 311
Fragments arranged by size:
1005 311 299 209 32 32 32 32
4
KasI G'GCGC_C
Cuts at: 0 187 1847 1956
Size: 187 1660 109
Fragments arranged by size:
1660 187 109
KpnI G_GTAC'C
Cuts at: 0 395 1956
Size: 395 1561
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1004 1352 1605 1781 1956
Size: 1004 348 253 176 175
Fragments arranged by size:
1004 348 253 176 175
MscI TGG'CCA
Cuts at: 0 4 1956
Size: 4 1952
MslI CAynn'nnrTG
Cuts at: 0 226 1744 1867 1956
Size: 226 1518 123 89
Fragments arranged by size:
1518 226 123 89
MspA1I CmG'CkG
Cuts at: 0 295 306 1709 1956
Size: 295 11 1403 247
Fragments arranged by size:
1403 295 247 11
NarI GG'CG_CC
Cuts at: 0 188 1848 1956
Size: 188 1660 108
Fragments arranged by size:
1660 188 108
NcoI C'CATG_G
Cuts at: 0 5 281 954 1673 1956
Size: 5 276 673 719 283
Fragments arranged by size:
719 673 283 276 5
NdeI CA'TA_TG
Cuts at: 0 1950 1956
Size: 1950 6
NsiI A_TGCA'T
Cuts at: 0 145 1956
Size: 145 1811
NspI r_CATG'y
Cuts at: 0 143 1956
Size: 143 1813
PflMI CCAn_nnn'nTGG
Cuts at: 0 1674 1956
Size: 1674 282
PpuMI rG'GwC_Cy
Cuts at: 0 887 1404 1473 1493 1683 1956
Size: 887 517 69 20 190 273
Fragments arranged by size:
887 517 273 190 69 20
PspOMI G'GGCC_C
Cuts at: 0 1095 1956
Size: 1095 861
PspXI vC'TCGA_Gb
Cuts at: 0 1299 1956
Size: 1299 657
PstI C_TGCA'G
Cuts at: 0 1413 1472 1956
Size: 1413 59 484
Fragments arranged by size:
1413 484 59
PvuI CG_AT'CG
Cuts at: 0 1721 1956
Size: 1721 235
PvuII CAG'CTG
Cuts at: 0 306 1956
Size: 306 1650
SapI GCTCTTCn'nnn_
Cuts at: 0 700 930 1931 1956
Size: 700 230 1001 25
Fragments arranged by size:
1001 700 230 25
SbfI CC_TGCA'GG
Cuts at: 0 1472 1956
Size: 1472 484
SexAI A'CCwGG_T
Cuts at: 0 822 1956
Size: 822 1134
SfcI C'TryA_G
Cuts at: 0 365 1409 1468 1956
Size: 365 1044 59 488
Fragments arranged by size:
1044 488 365 59
SfoI GGC'GCC
Cuts at: 0 189 1849 1956
Size: 189 1660 107
Fragments arranged by size:
1660 189 107
SmlI C'TyrA_G
Cuts at: 0 129 1299 1956
Size: 129 1170 657
Fragments arranged by size:
1170 657 129
SphI G_CATG'C
Cuts at: 0 143 1956
Size: 143 1813
SspI AAT'ATT
Cuts at: 0 158 1956
Size: 158 1798
StuI AGG'CCT
Cuts at: 0 1640 1956
Size: 1640 316
StyI C'CwwG_G
Cuts at: 0 5 281 830 954 1673 1956
Size: 5 276 549 124 719 283
Fragments arranged by size:
719 549 283 276 124 5
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1522 1956
Size: 1522 434
TatI w'GTAC_w
Cuts at: 0 236 486 596 1956
Size: 236 250 110 1360
Fragments arranged by size:
1360 250 236 110
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 596 628 1956
Size: 596 32 1328
Fragments arranged by size:
1328 596 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1536 1956
Size: 1536 420
XhoI C'TCGA_G
Cuts at: 0 1299 1956
Size: 1299 657
XmnI GAAnn'nnTTC
Cuts at: 0 206 1864 1956
Size: 206 1658 92
Fragments arranged by size:
1658 206 92
Enzymes that do cut:
AarI Acc65I AfeI AhdI AleI AlfI ApaI ApaLI
ApoI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BfrBI BglII Bme1580I BmgBI BmrI BplI Bpu10I
BpuEI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstEII BstYI Bsu36I BtgI BtsI DraI DraIII EaeI
EarI EciI EcoNI EcoO109I HaeII Hin4I KasI KpnI
MmeI MscI MslI MspA1I NarI NcoI NdeI NsiI
NspI PflMI PpuMI PspOMI PspXI PstI PvuI PvuII
SapI SbfI SexAI SfcI SfoI SmlI SphI SspI
StuI StyI TaqII TatI TstI XcmI XhoI XmnI
Enzymes that do not cut:
AatII AccI AclI AcuI AflII AflIII AgeI AloI
AlwNI AscI AseI AsiSI AvrII BcgI BciVI BclI
BglI BlpI BmtI BpmI BsaI BsaAI BsaBI BsaWI
BsiWI BsmI BspEI BspHI BssHII BstAPI BstBI BstXI
BstZ17I BtgZI ClaI CspCI DrdI EagI EcoICRI Eco57MI
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI MfeI MluI NaeI NgoMIV NheI NotI NruI
PacI PasI PciI PfoI PmeI PmlI PpiI PshAI
PsiI PsrI RsrII SacI SacII SalI SanDI ScaI
SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI SwaI
Tth111I XbaI XmaI ZraI