Restriction map for
pFN21AB0039
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# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII AccI AccII AccIII AcyI
AflII AflIII AgeI AhdI Aor51HI
ApaI AscI AseI AsnI Asp700I
AspEI AviII AvrII BalI BamHI
BbeI BbrPI BcgI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BssHII BssSI Bst1107I
BstEII BstPI BstUI BstZ17I CelII
Cfr10I ClaI CpoI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRV FseI FspI HaeII HgaI
Hin1I HindIII HpaI KasI KspI
MamI MfeI MluI MluNI MroI
MscI MspA1I MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NspI PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
PvuI PvuII RcaI RsrII SacI
SacII SalI SbfI ScaI SfiI
SfoI SgrAI SmaI SmlI SnaBI
SphI Sse8387I SspI SwaI XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 916 GGTACC G^GTACC
AclI 1508 AACGTT AA^CGTT
Alw44I 432 GTGCAC G^TGCAC
AlwI 42 GGATC GGATC(4/5)
AocI 222 CCTAAGG CC^T[ACGT]AGG
ApaLI 432 GTGCAC G^TGCAC
Asp718I 916 GGTACC G^GTACC
AspI 156 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1167 CTCGGG C^[CT]CG[GA]G
BanII 1025 GAGCCC G[GA]GC[CT]^C
BbsI 394 GAAGAC GAAGAC(2/6)
BbvCI 948 CCTCAGC CCTCAGC(-5/-2)
BciVI 1172 GTATCC GTATCC(6/5)
BclI 1317 TGATCA T^GATCA
BcnI 1133 CCGGG CC^[GC]GG
BglII 114 AGATCT A^GATCT
BpuAI 394 GAAGAC GAAGAC(2/6)
BseRI 795 CTCCTC GAGGAG(10/8)
BsmAI 70 GTCTC GTCTC(1/5)
BsmBI 69 CGTCTC CGTCTC(1/5)
BsoBI 1167 CTCGGG C^[CT]CG[GA]G
Bsp1407I 1208 TGTACA T^GTACA
BsrGI 1208 TGTACA T^GTACA
BstYI 114 AGATCT [GA]^GATC[CT]
Bsu36I 222 CCTAAGG CC^T[ACGT]AGG
DraI 1092 TTTAAA TTT^AAA
DraIII 908 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 445 GACATCCGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 222 CCTAAGG CC^T[ACGT]AGG
EcoRI 57 GAATTC G^AATTC
EcoT22I 343 ATGCAT ATGCA^T
FbaI 1317 TGATCA T^GATCA
HincII 1003 GTCAAC GT[CT]^[GA]AC
HindII 1003 GTCAAC GT[CT]^[GA]AC
KpnI 916 GGTACC GGTAC^C
MflI 114 AGATCT [GA]^GATC[CT]
MslI 710 CATCTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 1133 CCGGG CC^[GC]GG
NsiI 343 ATGCAT ATGCA^T
PflFI 156 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 828 CCACCCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 452 GAGTC GAGTC(4/5)
Psp1406I 1508 AACGTT AA^CGTT
SapI 313 GCTCTTC GCTCTTC(1/4)
SexAI 157 ACCTGGT A^CC[AT]GGT
SpeI 961 ACTAGT A^CTAGT
SspBI 1208 TGTACA T^GTACA
StuI 1160 AGGCCT AGG^CCT
TfiI 1218 GAATC G^A[AT]TC
Tsp45I 535 GTGAC ^GT[GC]AC
Tth111I 156 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 828 CCACCCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 114 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AfaI 917 GTAC GT^AC
1209 GTAC
AlwNI 707 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
1321 CAGGACCTG
BpmI 679 CTCCAG CTGGAG(16/14)
1043 CTGGAG
BstBI 187 TTCGAA TT^CGAA
1512 TTCGAA
BstXI 151 CCAAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1305 CCAGGAGTTTGG
CfoI 704 GCGC GCG^C
1195 GCGC
EcoNI 1152 CCTCACTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1299 CCTGCCCCAGG
HapII 489 CCGG C^CGG
1133 CCGG
HhaI 704 GCGC GCG^C
1195 GCGC
HinP1I 704 GCGC G^CGC
1195 GCGC
HinfI 452 GAGTC G^A[ACGT]TC
1218 GAATC
HpaII 489 CCGG C^CGG
1133 CCGG
MaeII 218 ACGT A^CGT
1509 ACGT
MspI 489 CCGG C^CGG
1133 CCGG
NspV 187 TTCGAA TT^CGAA
1512 TTCGAA
RsaI 917 GTAC GT^AC
1209 GTAC
SfuI 187 TTCGAA TT^CGAA
1512 TTCGAA
TaqI 188 TCGA T^CGA
1513 TCGA
TthHB8I 188 TCGA T^CGA
1513 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AcsI AluI ApoI AspHI
AvaII BanI BbvI BfaI BmyI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmFI BsmI Bsp1286I BspMI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraII
DsaI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MnlI MseI MvaI MwoI
NcoI NdeII NlaIII NlaIV PpuMI
PstI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI Tru9I TseI Tsp509I
TspRI