(Circular) (Six-base) MAPSORT of: pFN21AB0039.seq Check: 9451 from: 1 to: 6241 pFN21AB0039 6241 bp With 182 enzymes: SgfI * February 27, 2009 14:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3312 3312 Size: 6241 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4051 278 Size: 53 83 186 936 2515 2468 Fragments arranged by size: 2515 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3584 1937 Size: 1647 4594 Acc65I G'GTAC_C Cuts at: 2921 3562 2921 Size: 641 5600 AclI AA'CG_TT Cuts at: 1563 3514 4368 4741 1563 Size: 1951 854 373 3063 Fragments arranged by size: 3063 1951 854 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2771 4304 5688 5956 1632 Size: 1139 1533 1384 268 1917 Fragments arranged by size: 1917 1533 1384 1139 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6044 AflIII A'CryG_T Cuts at: 1203 5140 6117 1203 Size: 3937 977 1327 Fragments arranged by size: 3937 1327 977 AgeI A'CCGG_T Cuts at: 5072 5072 Size: 6241 AhdI GACnn_n'nnGTC Cuts at: 4970 4970 Size: 6241 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6241 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3207 1184 Size: 2023 4218 AlwNI CAG_nnn'CTG Cuts at: 2717 3331 3984 5556 2717 Size: 614 653 1572 3402 Fragments arranged by size: 3402 1572 653 614 ApaLI G'TGCA_C Cuts at: 2437 4298 5454 2437 Size: 1861 1156 3224 Fragments arranged by size: 3224 1861 1156 ApoI r'AATT_y Cuts at: 1493 2062 2633 2869 3550 3776 3928 6037 1493 Size: 569 571 236 681 226 152 2109 1697 Fragments arranged by size: 2109 1697 681 571 569 236 226 152 AseI AT'TA_AT Cuts at: 160 1032 4795 160 Size: 872 3763 1606 Fragments arranged by size: 3763 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6241 AvaI C'yCGr_G Cuts at: 1870 1943 3172 3566 1870 Size: 73 1229 394 4545 Fragments arranged by size: 4545 1229 394 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3160 3193 3160 Size: 33 6208 BamHI G'GATC_C Cuts at: 3571 3938 3571 Size: 367 5874 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2093 2921 3134 3562 5017 Size: 325 217 627 306 828 213 428 1455 Cuts at: 5017 618 Size: 1842 Fragments arranged by size: 1842 1455 828 627 428 325 306 217 213 BanII G_rGCy'C Cuts at: 1391 3034 3560 1391 Size: 1643 526 4072 Fragments arranged by size: 4072 1643 526 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2406 928 Size: 971 507 4763 Fragments arranged by size: 4763 971 507 BbvCI CC'TCA_GC Cuts at: 2954 2954 Size: 6241 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4432 4466 1903 Size: 34 2495 34 3678 Fragments arranged by size: 3678 2495 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3188 4133 5343 3188 Size: 945 1210 4086 Fragments arranged by size: 4086 1210 945 BclI T'GATC_A Cuts at: 1548 3322 1548 Size: 1774 4467 BfrBI ATG'CAT Cuts at: 2350 2350 Size: 6241 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4852 136 Size: 107 122 71 631 605 3180 1525 Fragments arranged by size: 3180 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2119 6236 2119 Size: 4117 2124 BlpI GC'TnA_GC Cuts at: 3658 3658 Size: 6241 Bme1580I G_kGCm'C Cuts at: 1790 2441 4302 5458 1790 Size: 651 1861 1156 2573 Fragments arranged by size: 2573 1861 1156 651 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6241 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2752 3972 4930 Size: 416 53 83 399 24 1335 1220 958 Cuts at: 4930 442 Size: 1753 Fragments arranged by size: 1753 1335 1220 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6241 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2629 2629 Size: 6241 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2669 3069 4901 1239 Size: 1430 400 1832 2579 Fragments arranged by size: 2579 1832 1430 400 Bpu10I CC'TnA_GC Cuts at: 2519 2822 2954 3401 2519 Size: 303 132 447 5359 Fragments arranged by size: 5359 447 303 132 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4366 5231 5529 4366 Size: 865 298 5078 Fragments arranged by size: 5078 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4904 882 Size: 617 3405 2219 Fragments arranged by size: 3405 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4032 493 Size: 711 2828 2702 Fragments arranged by size: 2828 2702 711 BsaBI GATnn'nnATC Cuts at: 6235 6235 Size: 6241 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4048 4430 5065 275 Size: 53 83 186 936 2515 382 635 1451 Fragments arranged by size: 2515 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4674 5072 5346 5493 5904 4674 Size: 398 274 147 411 5011 Fragments arranged by size: 5011 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6016 Fragments arranged by size: 6016 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2791 1186 Size: 1605 4636 BseYI C'CCAG_C Cuts at: 2676 5444 5940 2676 Size: 2768 496 2977 Fragments arranged by size: 2977 2768 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2862 2919 3524 1163 Size: 573 1126 57 605 3880 Fragments arranged by size: 3880 1126 605 573 57 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3691 4452 5480 1540 Size: 275 186 1690 761 1028 2301 Fragments arranged by size: 2301 1690 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2441 2964 3146 4302 4387 5458 2441 Size: 523 182 1156 85 1071 3224 Fragments arranged by size: 3224 1156 1071 523 182 85 BsmI GAATG_Cn' Cuts at: 2320 2495 2857 3752 3845 2320 Size: 175 362 895 93 4716 Fragments arranged by size: 4716 895 362 175 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2080 2080 Size: 6241 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2441 2964 3034 3146 3560 4302 4387 Size: 399 651 523 70 112 414 742 85 Cuts at: 4387 5458 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 651 523 414 399 112 85 70 BspEI T'CCGG_A Cuts at: 5904 5904 Size: 6241 BspHI T'CATG_A Cuts at: 4130 5860 4130 Size: 1730 4511 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2380 3312 3339 3596 844 Size: 1536 932 27 257 3489 Fragments arranged by size: 3489 1536 932 257 27 BsrBI CCG'CTC Cuts at: 1394 1823 4128 1394 Size: 429 2305 3507 Fragments arranged by size: 3507 2305 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2050 2589 2862 4736 4910 65 Size: 1183 802 539 273 1874 174 1396 Fragments arranged by size: 1874 1396 1183 802 539 273 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4885 5072 1700 Size: 110 3075 187 2869 Fragments arranged by size: 3075 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3213 96 Size: 3117 3124 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6241 BssSI C'ACGA_G Cuts at: 4301 5313 4301 Size: 1012 5229 BstAPI GCAn_nnn'nTGC Cuts at: 794 2265 2695 2885 2897 794 Size: 1471 430 190 12 4138 Fragments arranged by size: 4138 1471 430 190 12 BstBI TT'CG_AA Cuts at: 2193 3518 5045 2193 Size: 1325 1527 3389 Fragments arranged by size: 3389 1527 1325 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6241 BstXI CCAn_nnnn'nTGG Cuts at: 2163 3317 2163 Size: 1154 5087 BstYI r'GATC_y Cuts at: 1972 2119 3571 3938 4323 4340 5792 5878 5890 Size: 147 1452 367 385 17 1452 86 12 Cuts at: 5890 6236 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1452 385 367 346 147 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2228 2228 Size: 6241 BtgI C'CryG_G Cuts at: 513 1065 2004 2559 2601 2622 3147 513 Size: 552 939 555 42 21 525 3607 Fragments arranged by size: 3607 939 555 552 525 42 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5502 Fragments arranged by size: 5502 683 56 BtsI GCAGTG_nn' Cuts at: 3124 3757 4551 4571 3124 Size: 633 794 20 4794 Fragments arranged by size: 4794 794 633 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6241 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6206 DraI TTT'AAA Cuts at: 3099 3545 3099 Size: 446 5795 DraIII CAC_nnn'GTG Cuts at: 2918 2918 Size: 6241 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2456 5248 809 Size: 507 1140 2792 1802 Fragments arranged by size: 2792 1802 1140 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3688 4577 6206 Size: 54 1425 50 275 18 1858 889 1629 Cuts at: 6206 8 Size: 43 Fragments arranged by size: 1858 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3688 1537 Size: 275 1876 4090 Fragments arranged by size: 4090 1876 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2195 2325 2473 2660 4171 1353 Size: 842 130 148 187 1511 3423 Fragments arranged by size: 3423 1511 842 187 148 130 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4828 5202 5348 387 Size: 1064 138 3239 374 146 1280 Fragments arranged by size: 3239 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2669 2771 3069 4304 4901 5688 5956 Size: 393 1037 102 298 1235 597 787 268 Cuts at: 5956 1239 Size: 1524 Fragments arranged by size: 1524 1235 1037 787 597 393 298 268 102 EcoNI CCTnn'n_nnAGG Cuts at: 3161 3308 3161 Size: 147 6094 EcoO109I rG'GnC_Cy Cuts at: 2160 2507 2976 3154 3328 2160 Size: 347 469 178 174 5073 Fragments arranged by size: 5073 469 347 178 174 EcoRI G'AATT_C Cuts at: 2062 3550 3928 2062 Size: 1488 378 4375 Fragments arranged by size: 4375 1488 378 FspI TGC'GCA Cuts at: 4747 4747 Size: 6241 HaeII r_GCGC'y Cuts at: 1678 5388 1678 Size: 3710 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2032 2039 2064 2071 4882 Size: 32 135 32 460 7 25 7 2811 Cuts at: 4882 4914 4956 4988 1373 Size: 32 42 32 2626 Fragments arranged by size: 2811 2626 460 135 42 32 32 32 32 25 7 7 HincII GTy'rAC Cuts at: 669 1657 1938 3010 3585 3831 669 Size: 988 281 1072 575 246 3079 Fragments arranged by size: 3079 1072 988 575 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6241 HpaI GTT'AAC Cuts at: 3831 3831 Size: 6241 KpnI G_GTAC'C Cuts at: 2925 3566 2925 Size: 641 5600 MfeI C'AATT_G Cuts at: 3840 3840 Size: 6241 MluI A'CGCG_T Cuts at: 6117 6117 Size: 6241 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2001 5355 5539 1305 Size: 696 3354 184 2007 Fragments arranged by size: 3354 2007 696 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4419 Fragments arranged by size: 4419 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2719 4199 4558 4717 518 Size: 648 1553 1480 359 159 2042 Fragments arranged by size: 2042 1553 1480 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3657 4334 5482 5727 1606 Size: 2051 677 1148 245 2120 Fragments arranged by size: 2120 2051 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2559 2601 2622 3147 513 Size: 552 939 555 42 21 525 3607 Fragments arranged by size: 3607 939 555 552 525 42 21 NdeI CA'TA_TG Cuts at: 387 387 Size: 6241 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6241 NotI GC'GGCC_GC Cuts at: 3688 3688 Size: 6241 NsiI A_TGCA'T Cuts at: 2352 2352 Size: 6241 NspI r_CATG'y Cuts at: 1132 1229 3599 5144 1132 Size: 97 2370 1545 2229 Fragments arranged by size: 2370 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2342 1348 Size: 994 5247 PciI A'CATG_T Cuts at: 5140 5140 Size: 6241 PflMI CCAn_nnn'nTGG Cuts at: 1108 2839 1108 Size: 1731 4510 PmeI GTTT'AAAC Cuts at: 3545 3545 Size: 6241 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4310 4342 5823 5855 4310 Size: 32 1481 32 4696 Fragments arranged by size: 4696 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2160 2507 2976 3328 2160 Size: 347 469 352 5073 Fragments arranged by size: 5073 469 352 347 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6241 PsiI TTA'TAA Cuts at: 3811 3811 Size: 6241 PstI C_TGCA'G Cuts at: 830 2893 3039 3339 3593 830 Size: 2063 146 300 254 3478 Fragments arranged by size: 3478 2063 300 254 146 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6241 SalI G'TCGA_C Cuts at: 1936 3583 1936 Size: 1647 4594 SapI GCTCTTCn'nnn_ Cuts at: 2325 2325 Size: 6241 SbfI CC_TGCA'GG Cuts at: 3593 3593 Size: 6241 ScaI AGT'ACT Cuts at: 4489 6201 4489 Size: 1712 4529 SexAI A'CCwGG_T Cuts at: 2162 2162 Size: 6241 SfcI C'TryA_G Cuts at: 826 1045 2889 3035 3335 3589 4724 5405 5596 Size: 219 1844 146 300 254 1135 681 191 Cuts at: 5596 826 Size: 1471 Fragments arranged by size: 1844 1471 1135 681 300 254 219 191 146 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6241 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6241 SmaI CCC'GGG Cuts at: 1872 3568 1872 Size: 1696 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4345 5246 5508 820 Size: 197 926 2402 901 262 1553 Fragments arranged by size: 2402 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4032 493 Size: 3539 2702 SpeI A'CTAG_T Cuts at: 152 2966 152 Size: 2814 3427 SphI G_CATG'C Cuts at: 1132 3599 1132 Size: 2467 3774 SspI AAT'ATT Cuts at: 5 52 4165 5 Size: 47 4113 2081 Fragments arranged by size: 4113 2081 47 StuI AGG'CCT Cuts at: 1517 3167 1517 Size: 1650 4591 StyI C'CwwG_G Cuts at: 513 1065 2004 2156 2559 2601 2622 3147 3372 Size: 552 939 152 403 42 21 525 225 Cuts at: 3372 3680 513 Size: 308 3074 Fragments arranged by size: 3074 939 552 525 403 308 225 152 42 21 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2121 2635 2850 4268 4285 4438 4623 Size: 1255 541 514 215 1418 17 153 185 Cuts at: 4623 5068 325 Size: 445 1498 Fragments arranged by size: 1498 1418 1255 541 514 445 215 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3213 4487 6199 Size: 274 80 33 51 1443 1236 1274 1712 Cuts at: 6199 96 Size: 138 Fragments arranged by size: 1712 1443 1274 1236 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6201 6233 6201 Size: 32 6209 Tth111I GACn'n_nGTC Cuts at: 1316 2164 1316 Size: 848 5393 XbaI T'CTAG_A Cuts at: 3577 3577 Size: 6241 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6241 XmaI C'CCGG_G Cuts at: 1870 3566 1870 Size: 1696 4545 XmnI GAAnn'nnTTC Cuts at: 4370 4370 Size: 6241 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4049 276 Size: 53 83 186 936 2515 2468 Fragments arranged by size: 2515 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BsiWI BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI XcmI