Restriction map for
pFN21AB0039
(Linear) (Six-base) MAPSORT of: KIBB0039.seq Check: 5765 from: 1 to: 1536
KIBB0039.orf
With 182 enzymes: SgfI *
July 18, 2007 13:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1307 1536
Size: 1307 229
Acc65I G'GTAC_C
Cuts at: 0 916 1536
Size: 916 620
AclI AA'CG_TT
Cuts at: 0 1509 1536
Size: 1509 27
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 766 1536
Size: 766 770
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1202 1536
Size: 1202 334
AlwNI CAG_nnn'CTG
Cuts at: 0 712 1326 1536
Size: 712 614 210
Fragments arranged by size:
712 614 210
ApaLI G'TGCA_C
Cuts at: 0 432 1536
Size: 432 1104
ApoI r'AATT_y
Cuts at: 0 57 628 864 1536
Size: 57 571 236 672
Fragments arranged by size:
672 571 236 57
AvaI C'yCGr_G
Cuts at: 0 1167 1536
Size: 1167 369
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1155 1188 1536
Size: 1155 33 348
Fragments arranged by size:
1155 348 33
BanI G'GyrC_C
Cuts at: 0 88 916 1129 1536
Size: 88 828 213 407
Fragments arranged by size:
828 407 213 88
BanII G_rGCy'C
Cuts at: 0 1029 1536
Size: 1029 507
BbsI GAAGACnn'nnnn_
Cuts at: 0 401 1536
Size: 401 1135
BbvCI CC'TCA_GC
Cuts at: 0 949 1536
Size: 949 587
BciVI GTATCCnnnnn_n'
Cuts at: 0 1183 1536
Size: 1183 353
BclI T'GATC_A
Cuts at: 0 1317 1536
Size: 1317 219
BfrBI ATG'CAT
Cuts at: 0 345 1536
Size: 345 1191
BglII A'GATC_T
Cuts at: 0 114 1536
Size: 114 1422
Bme1580I G_kGCm'C
Cuts at: 0 436 1536
Size: 436 1100
BmrI ACTGGGnnnn_n'
Cuts at: 0 747 1536
Size: 747 789
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 624 1536
Size: 624 912
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 664 1064 1536
Size: 664 400 472
Fragments arranged by size:
664 472 400
Bpu10I CC'TnA_GC
Cuts at: 0 514 817 949 1396 1536
Size: 514 303 132 447 140
Fragments arranged by size:
514 447 303 140 132
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 786 1536
Size: 786 750
BseYI C'CCAG_C
Cuts at: 0 671 1536
Size: 671 865
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 857 914 1519 1536
Size: 857 57 605 17
Fragments arranged by size:
857 605 57 17
BsiHKAI G_wGCw'C
Cuts at: 0 436 959 1141 1536
Size: 436 523 182 395
Fragments arranged by size:
523 436 395 182
BsmI GAATG_Cn'
Cuts at: 0 315 490 852 1536
Size: 315 175 362 684
Fragments arranged by size:
684 362 315 175
BsmBI CGTCTCn'nnnn_
Cuts at: 0 75 1536
Size: 75 1461
Bsp1286I G_dGCh'C
Cuts at: 0 436 959 1029 1141 1536
Size: 436 523 70 112 395
Fragments arranged by size:
523 436 395 112 70
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 375 1307 1334 1536
Size: 375 932 27 202
Fragments arranged by size:
932 375 202 27
BsrDI GCAATG_nn'
Cuts at: 0 45 584 857 1536
Size: 45 539 273 679
Fragments arranged by size:
679 539 273 45
BsrGI T'GTAC_A
Cuts at: 0 1208 1536
Size: 1208 328
BstAPI GCAn_nnn'nTGC
Cuts at: 0 260 690 880 892 1536
Size: 260 430 190 12 644
Fragments arranged by size:
644 430 260 190 12
BstBI TT'CG_AA
Cuts at: 0 188 1513 1536
Size: 188 1325 23
Fragments arranged by size:
1325 188 23
BstXI CCAn_nnnn'nTGG
Cuts at: 0 158 1312 1536
Size: 158 1154 224
Fragments arranged by size:
1154 224 158
BstYI r'GATC_y
Cuts at: 0 114 1536
Size: 114 1422
Bsu36I CC'TnA_GG
Cuts at: 0 223 1536
Size: 223 1313
BtgI C'CryG_G
Cuts at: 0 554 596 617 1142 1536
Size: 554 42 21 525 394
Fragments arranged by size:
554 525 394 42 21
BtsI GCAGTG_nn'
Cuts at: 0 1119 1536
Size: 1119 417
DraI TTT'AAA
Cuts at: 0 1094 1536
Size: 1094 442
DraIII CAC_nnn'GTG
Cuts at: 0 913 1536
Size: 913 623
DrdI GACnn_nn'nnGTC
Cuts at: 0 451 1536
Size: 451 1085
EarI CTCTTCn'nnn_
Cuts at: 0 190 320 468 655 1536
Size: 190 130 148 187 881
Fragments arranged by size:
881 190 187 148 130
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 664 766 1064 1536
Size: 664 102 298 472
Fragments arranged by size:
664 472 298 102
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1156 1303 1536
Size: 1156 147 233
Fragments arranged by size:
1156 233 147
EcoO109I rG'GnC_Cy
Cuts at: 0 155 502 971 1149 1323 1536
Size: 155 347 469 178 174 213
Fragments arranged by size:
469 347 213 178 174 155
EcoRI G'AATT_C
Cuts at: 0 57 1536
Size: 57 1479
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 27 34 59 66 1536
Size: 27 7 25 7 1470
Fragments arranged by size:
1470 27 25 7 7
HincII GTy'rAC
Cuts at: 0 1005 1536
Size: 1005 531
KpnI G_GTAC'C
Cuts at: 0 920 1536
Size: 920 616
MslI CAynn'nnrTG
Cuts at: 0 714 1536
Size: 714 822
NcoI C'CATG_G
Cuts at: 0 554 596 617 1142 1536
Size: 554 42 21 525 394
Fragments arranged by size:
554 525 394 42 21
NsiI A_TGCA'T
Cuts at: 0 347 1536
Size: 347 1189
PasI CC'CwG_GG
Cuts at: 0 337 1536
Size: 337 1199
PflMI CCAn_nnn'nTGG
Cuts at: 0 834 1536
Size: 834 702
PpuMI rG'GwC_Cy
Cuts at: 0 155 502 971 1323 1536
Size: 155 347 469 352 213
Fragments arranged by size:
469 352 347 213 155
PstI C_TGCA'G
Cuts at: 0 888 1034 1334 1536
Size: 888 146 300 202
Fragments arranged by size:
888 300 202 146
SapI GCTCTTCn'nnn_
Cuts at: 0 320 1536
Size: 320 1216
SexAI A'CCwGG_T
Cuts at: 0 157 1536
Size: 157 1379
SfcI C'TryA_G
Cuts at: 0 884 1030 1330 1536
Size: 884 146 300 206
Fragments arranged by size:
884 300 206 146
SpeI A'CTAG_T
Cuts at: 0 961 1536
Size: 961 575
StuI AGG'CCT
Cuts at: 0 1162 1536
Size: 1162 374
StyI C'CwwG_G
Cuts at: 0 151 554 596 617 1142 1367 1536
Size: 151 403 42 21 525 225 169
Fragments arranged by size:
525 403 225 169 151 42 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 116 630 845 1536
Size: 116 514 215 691
Fragments arranged by size:
691 514 215 116
TatI w'GTAC_w
Cuts at: 0 1208 1536
Size: 1208 328
Tth111I GACn'n_nGTC
Cuts at: 0 159 1536
Size: 159 1377
Enzymes that do cut:
AarI Acc65I AclI AcuI AlfI AlwNI ApaLI ApoI
AvaI BaeI BanI BanII BbsI BbvCI BciVI BclI
BfrBI BglII Bme1580I BmrI BplI BpmI Bpu10I BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrDI
BsrGI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtsI
DraI DraIII DrdI EarI Eco57MI EcoNI EcoO109I EcoRI
Hin4I HincII KpnI MslI NcoI NsiI PasI PflMI
PpuMI PstI SapI SexAI SfcI SpeI StuI StyI
TaqII TatI Tth111I
Enzymes that do not cut:
AatII AccI AfeI AflII AflIII AgeI AhdI AleI
AloI ApaI AscI AseI AsiSI AvrII BamHI BbeI
BcgI BglI BlpI BmgBI BmtI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI BspEI BspHI
BsrBI BsrFI BssHII BssSI BstEII BstZ17I BtgZI ClaI
CspCI EaeI EagI EciI EcoICRI EcoRV FalI FseI
FspI FspAI HaeII HindIII HpaI KasI MfeI MluI
MmeI MscI MspA1I NaeI NarI NdeI NgoMIV NheI
NotI NruI NspI PacI PciI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SphI SrfI
SspI SwaI TstI XbaI XcmI XhoI XmaI XmnI
ZraI