################################## # NO CUT ENZYMES ( 47 enzymes) # ################################## AatII AclI AgeI AhdI Aor51HI AscI AspEI AvrII BbrPI BlnI BsiEI BsiWI BsmBI BspDI BsrBI BssHII Bst1107I BstZ17I ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I FseI MluI NotI PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SbfI SfiI SnaBI Sse8387I SspI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 64 enzymes) # ################################### Acc65I 6589 GGTACC G^GTACC AccIII 6059 TCCGGA T^CCGGA AflIII 731 ACATGT A^C[GA][CT]GT Alw44I 3399 GTGCAC G^TGCAC ApaI 243 GGGCCC GGGCC^C ApaLI 3399 GTGCAC G^TGCAC Asp700I 907 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 6589 GGTACC G^GTACC AspI 7174 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 128 CCCGGG C^[CT]CG[GA]G AviII 140 TGCGCA TGC^GCA BbeI 5648 GGCGCC GGCGC^C BbvCI 7012 GCTGAGG CCTCAGC(-5/-2) BcgI 6705 CGAGGCAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlpI 5812 GCTGAGC GC^T[ACGT]AGC Bpu1102I 5812 GCTGAGC GC^T[ACGT]AGC BsaAI 2303 TACGTG [CT]AC^GT[GA] BsaBI 1897 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 6059 TCCGGA [AT]^CCGG[AT] BseAI 6059 TCCGGA T^CCGGA BsoBI 128 CCCGGG C^[CT]CG[GA]G Bsp1407I 2007 TGTACA T^GTACA BspEI 6059 TCCGGA T^CCGGA BspLU11I 731 ACATGT A^CATGT BspMI 7324 GCAGGT ACCTGC(4/8) BsrGI 2007 TGTACA T^GTACA BstBI 1949 TTCGAA TT^CGAA BstEII 1123 GGTAACC G^GT[ACGT]ACC BstPI 1123 GGTAACC G^GT[ACGT]ACC CelII 5812 GCTGAGC GC^T[ACGT]AGC EcoO65I 1123 GGTAACC G^GT[ACGT]ACC EcoRI 632 GAATTC G^AATTC EcoRV 436 GATATC GAT^ATC FspI 140 TGCGCA TGC^GCA HgaI 204 GACGC GACGC(5/10) HpaI 955 GTTAAC GTT^AAC KasI 5648 GGCGCC G^GCGCC KpnI 6589 GGTACC GGTAC^C KspI 56 CCGCGG CCGC^GG MamI 1897 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 6059 TCCGGA T^CCGGA NaeI 37 GCCGGC GCC^GGC NarI 5648 GGCGCC GG^CGCC NdeI 692 CATATG CA^TATG NgoMIV 37 GCCGGC G^CCGGC NruI 20 TCGCGA TCG^CGA NspV 1949 TTCGAA TT^CGAA PflFI 7174 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 2441 CCAGGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SacI 6349 GAGCTC GAGCT^C SacII 56 CCGCGG CCGC^GG SexAI 3557 ACCTGGT A^CC[AT]GGT SfoI 5648 GGCGCC GGC^GCC SfuI 1949 TTCGAA TT^CGAA SgrAI 36 CGCCGGCG C[GA]^CCGG[CT]G SmaI 128 CCCGGG CCC^GGG SpeI 5482 ACTAGT A^CTAGT SphI 1376 GCATGC GCATG^C SspBI 2007 TGTACA T^GTACA Tth111I 7174 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 2441 CCAGGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 128 CCCGGG C^CCGGG XmaI 128 CCCGGG C^CCGGG XmnI 907 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccII 21 CGCG CG^CG 57 CGCG AcyI 204 GACGCC G[GA]^CG[CT]C 5648 GGCGCC BamHI 3333 GGATCC G^GATCC 6579 GGATCC BbsI 727 GAAGAC GAAGAC(2/6) 2863 GAAGAC BciVI 2916 GGATAC GTATCC(6/5) 4950 GGATAC BclI 1695 TGATCA T^GATCA 1704 TGATCA BglI 236 GCCTGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 5266 GCCCATGTGGC BpuAI 727 GAAGAC GAAGAC(2/6) 2863 GAAGAC BsaHI 204 GACGCC G[GA]^CG[CT]C 5648 GGCGCC BsaI 1366 GAGACC GGTCTC(1/5) 1427 GAGACC BsmI 2872 GAATGC GAATGC(1/-1) 3977 GCATTC BspHI 1514 TCATGA T^CATGA 1958 TCATGA BsrFI 37 GCCGGC [GA]^CCGG[CT] 5693 GCCGGT BssSI 6703 CACGAG CACGAG(-5/-1) 6154 CTCGTG BstUI 21 CGCG CG^CG 57 CGCG Cfr10I 37 GCCGGC [GA]^CCGG[CT] 5693 GCCGGT DraIII 1398 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG 6261 CACTCTGTG EcoT22I 1174 ATGCAT ATGCA^T 3521 ATGCAT FbaI 1695 TGATCA T^GATCA 1704 TGATCA Hin1I 204 GACGCC G[GA]^CG[CT]C 5648 GGCGCC MfeI 1825 CAATTG C^AATTG 2263 CAATTG MunI 1825 CAATTG C^AATTG 2263 CAATTG MvnI 21 CGCG CG^CG 57 CGCG NheI 3166 GCTAGC G^CTAGC 7320 GCTAGC NsiI 1174 ATGCAT ATGCA^T 3521 ATGCAT RcaI 1514 TCATGA T^CATGA 1958 TCATGA SalI 3119 GTCGAC G^TCGAC 5972 GTCGAC ScaI 558 AGTACT AGT^ACT 4178 AGTACT ######################################### # 3 OR MORE CUT ENZYMES ( 119 enzymes) # ######################################### AccI AciI AcsI AfaI AflII AluI AlwI AlwNI AocI ApoI AseI AsnI AspHI AvaII BalI BanI BanII BbvI BcnI BfaI BfrI BglII BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrDI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PshBI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII