(Circular) (Six-base) MAPSORT of: pFN21AA1840.seq Check: 725 from: 1 to: 12034 pFN21AA1840 12034 bp With 182 enzymes: SgfI * July 14, 2018 22:11 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 9320 9320 Size: 12034 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 9844 278 Size: 53 83 186 936 8308 2468 Fragments arranged by size: 8308 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3420 5025 5125 7978 9377 1937 Size: 1483 1605 100 2853 1399 4594 Fragments arranged by size: 4594 2853 1605 1483 1399 100 Acc65I G'GTAC_C Cuts at: 8594 9355 8594 Size: 761 11273 AclI AA'CG_TT Cuts at: 1563 10161 10534 1563 Size: 8598 373 3063 Fragments arranged by size: 8598 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3912 4111 4665 4724 6172 6525 6673 6729 Size: 2280 199 554 59 1448 353 148 56 Cuts at: 6729 6995 8240 8662 9218 9308 10097 11481 11749 Size: 266 1245 422 556 90 789 1384 268 Cuts at: 11749 1632 Size: 1917 Fragments arranged by size: 2280 1917 1448 1384 1245 789 556 554 422 353 268 266 199 148 90 59 56 AflII C'TTAA_G Cuts at: 820 1017 6211 8135 8801 820 Size: 197 5194 1924 666 4053 Fragments arranged by size: 5194 4053 1924 666 197 AflIII A'CryG_T Cuts at: 1203 2736 10933 11910 1203 Size: 1533 8197 977 1327 Fragments arranged by size: 8197 1533 1327 977 AgeI A'CCGG_T Cuts at: 10865 10865 Size: 12034 AhdI GACnn_n'nnGTC Cuts at: 10763 10763 Size: 12034 AleI CACnn'nnGTG Cuts at: 1166 3258 6482 1166 Size: 2092 3224 6718 Fragments arranged by size: 6718 3224 2092 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4746 7368 1184 Size: 3562 2622 5850 Fragments arranged by size: 5850 3562 2622 AlwNI CAG_nnn'CTG Cuts at: 3442 4452 8147 9777 11349 3442 Size: 1010 3695 1630 1572 4127 Fragments arranged by size: 4127 3695 1630 1572 1010 ApaI G_GGCC'C Cuts at: 2252 2252 Size: 12034 ApaLI G'TGCA_C Cuts at: 5404 10091 11247 5404 Size: 4687 1156 6191 Fragments arranged by size: 6191 4687 1156 ApoI r'AATT_y Cuts at: 1493 2354 2637 2860 3640 4183 4326 4526 4709 Size: 861 283 223 780 543 143 200 183 Cuts at: 4709 4818 5108 5596 6333 7213 7225 9051 9101 Size: 109 290 488 737 880 12 1826 50 Cuts at: 9101 9343 9569 9721 11830 1493 Size: 242 226 152 2109 1697 Fragments arranged by size: 2109 1826 1697 880 861 780 737 543 488 290 283 242 226 223 200 183 152 143 109 50 12 AseI AT'TA_AT Cuts at: 160 1032 3945 5663 6972 10588 160 Size: 872 2913 1718 1309 3616 1606 Fragments arranged by size: 3616 2913 1718 1606 1309 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 12034 AvaI C'yCGr_G Cuts at: 1870 1943 2133 9359 1870 Size: 73 190 7226 4545 Fragments arranged by size: 7226 4545 190 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2685 2718 2685 Size: 33 12001 BamHI G'GATC_C Cuts at: 5338 8584 9364 9731 5338 Size: 3246 780 367 7641 Fragments arranged by size: 7641 3246 780 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2057 2095 6440 7369 7653 Size: 325 217 627 270 38 4345 929 284 Cuts at: 7653 8594 8778 9355 10810 618 Size: 941 184 577 1455 1842 Fragments arranged by size: 4345 1842 1455 941 929 627 577 325 284 270 217 184 38 BanII G_rGCy'C Cuts at: 1391 2252 4567 5730 7464 8110 8358 9353 1391 Size: 861 2315 1163 1734 646 248 995 4072 Fragments arranged by size: 4072 2315 1734 1163 995 861 646 248 BbeI G_GCGC'C Cuts at: 7657 7657 Size: 12034 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2739 4875 928 Size: 971 840 2136 8087 Fragments arranged by size: 8087 2136 971 840 BbvCI CC'TCA_GC Cuts at: 9018 9018 Size: 12034 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 8699 8733 10225 10259 1903 Size: 34 6762 34 1492 34 3678 Fragments arranged by size: 6762 3678 1492 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4915 6949 9926 11136 4915 Size: 2034 2977 1210 5813 Fragments arranged by size: 5813 2977 2034 1210 BclI T'GATC_A Cuts at: 1548 3700 3709 1548 Size: 2152 9 9873 Fragments arranged by size: 9873 2152 9 BfrBI ATG'CAT Cuts at: 3181 5528 3181 Size: 2347 9687 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2247 7277 10645 Size: 107 122 71 631 605 575 5030 3368 Cuts at: 10645 136 Size: 1525 Fragments arranged by size: 5030 3368 1525 631 605 575 122 107 71 BglII A'GATC_T Cuts at: 2914 4278 7025 7404 7479 12029 2914 Size: 1364 2747 379 75 4550 2919 Fragments arranged by size: 4550 2919 2747 1364 379 75 BlpI GC'TnA_GC Cuts at: 7818 9451 7818 Size: 1633 10401 Bme1580I G_kGCm'C Cuts at: 1790 2060 2252 5408 6246 6287 7347 7374 7908 Size: 270 192 3156 838 41 1060 27 534 Cuts at: 7908 8781 10095 11251 1790 Size: 873 1314 1156 2573 Fragments arranged by size: 3156 2573 1314 1156 1060 873 838 534 270 192 41 27 BmgBI CAC'GTC Cuts at: 1318 7366 8391 1318 Size: 6048 1025 4961 Fragments arranged by size: 6048 4961 1025 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 6188 6844 8454 Size: 416 53 83 399 24 4771 656 1610 Cuts at: 8454 9765 10723 442 Size: 1311 958 1753 Fragments arranged by size: 4771 1753 1610 1311 958 656 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 5175 9329 1056 Size: 4119 4154 3761 Fragments arranged by size: 4154 4119 3761 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5694 6379 6624 6914 5694 Size: 685 245 290 10814 Fragments arranged by size: 10814 685 290 245 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2264 3086 4675 6419 6595 6743 6818 7406 Size: 1025 822 1589 1744 176 148 75 588 Cuts at: 7406 7415 7894 8661 10694 1239 Size: 9 479 767 2033 2579 Fragments arranged by size: 2579 2033 1744 1589 1025 822 767 588 479 176 148 75 9 Bpu10I CC'TnA_GC Cuts at: 8043 9018 8043 Size: 975 11059 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2784 3480 4178 4259 5786 6575 7847 8753 10159 Size: 696 698 81 1527 789 1272 906 1406 Cuts at: 10159 11024 11322 2784 Size: 865 298 3496 Fragments arranged by size: 3496 1527 1406 1272 906 865 789 698 696 298 81 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3365 3426 10697 882 Size: 617 1866 61 7271 2219 Fragments arranged by size: 7271 2219 1866 617 61 BsaAI yAC'GTr Cuts at: 493 1204 4310 9825 493 Size: 711 3106 5515 2702 Fragments arranged by size: 5515 3106 2702 711 BsaBI GATnn'nnATC Cuts at: 3906 12028 3906 Size: 8122 3912 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2210 7654 9841 10223 Size: 53 83 186 936 677 5444 2187 382 Cuts at: 10223 10858 275 Size: 635 1451 Fragments arranged by size: 5444 2187 1451 936 677 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 8064 10467 10865 11139 11286 11697 8064 Size: 2403 398 274 147 411 8401 Fragments arranged by size: 8401 2403 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 5232 5262 1178 Size: 30 165 30 3829 30 7950 Fragments arranged by size: 7950 3829 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2821 4175 5400 5722 6368 7328 7403 8480 Size: 1635 1354 1225 322 646 960 75 1077 Cuts at: 8480 1186 Size: 4740 Fragments arranged by size: 4740 1635 1354 1225 1077 960 646 322 75 BseYI C'CCAG_C Cuts at: 2052 3024 3153 3570 4719 5343 6157 6859 7425 Size: 972 129 417 1149 624 814 702 566 Cuts at: 7425 8868 11237 11733 2052 Size: 1443 2369 496 2353 Fragments arranged by size: 2369 2353 1443 1149 972 814 702 624 566 496 417 129 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2577 3167 3297 5872 6065 7769 1163 Size: 573 841 590 130 2575 193 1704 5428 Fragments arranged by size: 5428 2575 1704 841 590 573 193 130 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 9484 10245 11273 1540 Size: 275 186 7483 761 1028 2301 Fragments arranged by size: 7483 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3085 3584 5001 5408 7290 7753 8358 10095 10180 Size: 499 1417 407 1882 463 605 1737 85 Cuts at: 10180 11251 3085 Size: 1071 3868 Fragments arranged by size: 3868 1882 1737 1417 1071 605 499 463 407 85 BsmI GAATG_Cn' Cuts at: 4883 5982 9545 9638 4883 Size: 1099 3563 93 7279 Fragments arranged by size: 7279 3563 1099 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2060 2252 3085 3584 4567 5001 5408 Size: 399 270 192 833 499 983 434 407 Cuts at: 5408 5730 6246 6287 7290 7347 7374 7464 7753 Size: 322 516 41 1003 57 27 90 289 Cuts at: 7753 7908 8110 8358 8781 9353 10095 10180 11251 Size: 155 202 248 423 572 742 85 1071 Cuts at: 11251 1391 Size: 2174 Fragments arranged by size: 2174 1071 1003 983 833 742 572 516 499 434 423 407 399 322 289 270 248 202 192 155 90 85 57 41 27 BspEI T'CCGG_A Cuts at: 8064 11697 8064 Size: 3633 8401 BspHI T'CATG_A Cuts at: 3519 3963 9923 11653 3519 Size: 444 5960 1730 3900 Fragments arranged by size: 5960 3900 1730 444 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 9320 9389 844 Size: 8476 69 3489 Fragments arranged by size: 8476 3489 69 BsrBI CCG'CTC Cuts at: 1394 1823 9921 1394 Size: 429 8098 3507 Fragments arranged by size: 8098 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2414 5713 6516 8374 10529 10703 65 Size: 1183 1166 3299 803 1858 2155 174 1396 Fragments arranged by size: 3299 2155 1858 1396 1183 1166 803 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2042 7698 10678 10865 1700 Size: 110 232 5656 2980 187 2869 Fragments arranged by size: 5656 2980 2869 232 187 110 BsrGI T'GTAC_A Cuts at: 96 4012 96 Size: 3916 8118 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 12034 BssSI C'ACGA_G Cuts at: 8159 8708 10094 11106 8159 Size: 549 1386 1012 9087 Fragments arranged by size: 9087 1386 1012 549 BstAPI GCAn_nnn'nTGC Cuts at: 794 5005 6282 6939 794 Size: 4211 1277 657 5889 Fragments arranged by size: 5889 4211 1277 657 BstBI TT'CG_AA Cuts at: 3955 10838 3955 Size: 6883 5151 BstEII G'GTnAC_C Cuts at: 1184 3128 1184 Size: 1944 10090 BstXI CCAn_nnnn'nTGG Cuts at: 3544 6366 6796 7299 8014 3544 Size: 2822 430 503 715 7564 Fragments arranged by size: 7564 2822 715 503 430 BstYI r'GATC_y Cuts at: 1972 2667 2914 4278 5149 5338 6559 7025 7404 Size: 695 247 1364 871 189 1221 466 379 Cuts at: 7404 7479 7780 7794 8584 9364 9731 10116 10133 Size: 75 301 14 790 780 367 385 17 Cuts at: 10133 11585 11671 11683 12029 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1364 1221 871 790 780 695 466 385 379 367 346 301 247 189 86 75 17 14 12 Bsu36I CC'TnA_GG Cuts at: 2934 7759 7853 2934 Size: 4825 94 7115 Fragments arranged by size: 7115 4825 94 BtgI C'CryG_G Cuts at: 513 1065 2004 2061 2067 3086 3526 7960 8329 Size: 552 939 57 6 1019 440 4434 369 Cuts at: 8329 513 Size: 4218 Fragments arranged by size: 4434 4218 1019 939 552 440 369 57 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2129 487 Size: 683 56 903 10392 Fragments arranged by size: 10392 903 683 56 BtsI GCAGTG_nn' Cuts at: 2607 2823 3309 3392 6396 6845 7741 9550 10344 Size: 216 486 83 3004 449 896 1809 794 Cuts at: 10344 10364 2607 Size: 20 4277 Fragments arranged by size: 4277 3004 1809 896 794 486 449 216 83 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 12034 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 11999 DraI TTT'AAA Cuts at: 3510 4095 4203 4332 9338 3510 Size: 585 108 129 5006 6206 Fragments arranged by size: 6206 5006 585 129 108 DraIII CAC_nnn'GTG Cuts at: 3408 8271 3408 Size: 4863 7171 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 11041 809 Size: 507 9725 1802 Fragments arranged by size: 9725 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2064 2985 4833 Size: 54 1425 50 275 18 234 921 1848 Cuts at: 4833 5624 5826 7068 8926 9481 10370 11999 8 Size: 791 202 1242 1858 555 889 1629 43 Fragments arranged by size: 1858 1848 1629 1425 1242 921 889 791 555 275 234 202 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 9481 1537 Size: 275 7669 4090 Fragments arranged by size: 7669 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3495 3647 3741 5399 6728 6905 8163 9135 Size: 2142 152 94 1658 1329 177 1258 972 Cuts at: 9135 9964 1353 Size: 829 3423 Fragments arranged by size: 3423 2142 1658 1329 1258 972 829 177 152 94 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2029 10621 10995 11141 387 Size: 1064 138 440 8592 374 146 1280 Fragments arranged by size: 8592 1280 1064 440 374 146 138 EcoICRI GAG'CTC Cuts at: 8356 8356 Size: 12034 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2264 3086 3912 4111 4665 4675 4724 Size: 393 632 822 826 199 554 10 49 Cuts at: 4724 6172 6419 6525 6595 6673 6729 6743 6818 Size: 1448 247 106 70 78 56 14 75 Cuts at: 6818 6995 7406 7415 7894 8240 8661 8662 9218 Size: 177 411 9 479 346 421 1 556 Cuts at: 9218 9308 10097 10694 11481 11749 1239 Size: 90 789 597 787 268 1524 Fragments arranged by size: 1524 1448 826 822 789 787 632 597 556 554 479 421 411 393 346 268 247 199 177 106 90 78 75 70 56 49 14 10 9 1 EcoNI CCTnn'n_nnAGG Cuts at: 2925 5751 9300 2925 Size: 2826 3549 5659 Fragments arranged by size: 5659 3549 2826 EcoO109I rG'GnC_Cy Cuts at: 2248 2249 2930 6403 7984 8131 8806 9292 2248 Size: 1 681 3473 1581 147 675 486 4990 Fragments arranged by size: 4990 3473 1581 681 675 486 147 1 EcoRI G'AATT_C Cuts at: 2637 9343 9721 2637 Size: 6706 378 4950 Fragments arranged by size: 6706 4950 378 EcoRV GAT'ATC Cuts at: 2443 2443 Size: 12034 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3013 4300 5966 6099 6237 6734 8572 9089 3013 Size: 1287 1666 133 138 497 1838 517 5958 Fragments arranged by size: 5958 1838 1666 1287 517 497 138 133 FspI TGC'GCA Cuts at: 2147 10540 2147 Size: 8393 3641 HaeII r_GCGC'y Cuts at: 1678 4089 7321 7657 11181 1678 Size: 2411 3232 336 3524 2531 Fragments arranged by size: 3524 3232 2531 2411 336 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3211 3243 3507 3539 3604 Size: 32 135 32 1639 32 264 32 65 Cuts at: 3604 3636 3701 3733 4123 4155 5232 5264 6155 Size: 32 65 32 390 32 1077 32 891 Cuts at: 6155 6187 7616 7648 7786 7818 7971 8003 8731 Size: 32 1429 32 138 32 153 32 728 Cuts at: 8731 8763 10675 10707 10749 10781 1373 Size: 32 1912 32 42 32 2626 Fragments arranged by size: 2626 1912 1639 1429 1077 891 728 390 264 153 138 135 65 65 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2962 5126 7979 9378 9624 669 Size: 988 281 1024 2164 2853 1399 246 3079 Fragments arranged by size: 3079 2853 2164 1399 1024 988 281 246 HindIII A'AGCT_T Cuts at: 748 4397 6824 6866 6926 748 Size: 3649 2427 42 60 5856 Fragments arranged by size: 5856 3649 2427 60 42 HpaI GTT'AAC Cuts at: 2962 9624 2962 Size: 6662 5372 KasI G'GCGC_C Cuts at: 7653 7653 Size: 12034 KpnI G_GTAC'C Cuts at: 8598 9359 8598 Size: 761 11273 MfeI C'AATT_G Cuts at: 3830 4268 9633 3830 Size: 438 5365 6231 Fragments arranged by size: 6231 5365 438 MluI A'CGCG_T Cuts at: 11910 11910 Size: 12034 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2859 8286 8562 8752 8802 11148 11332 1305 Size: 1554 5427 276 190 50 2346 184 2007 Fragments arranged by size: 5427 2346 2007 1554 276 190 184 50 MscI TGG'CCA Cuts at: 10 64 1489 1832 2987 4835 5828 7070 8928 Size: 54 1425 343 1155 1848 993 1242 1858 Cuts at: 8928 10 Size: 3116 Fragments arranged by size: 3116 1858 1848 1425 1242 1155 993 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3258 3531 3564 4020 4728 6482 9185 Size: 648 2092 273 33 456 708 1754 2703 Cuts at: 9185 9992 10351 10510 518 Size: 807 359 159 2042 Fragments arranged by size: 2703 2092 2042 1754 807 708 648 456 359 273 159 33 MspA1I CmG'CkG Cuts at: 1606 2063 2143 3442 3628 5689 8260 8717 8785 Size: 457 80 1299 186 2061 2571 457 68 Cuts at: 8785 8881 9450 10127 11275 11520 1606 Size: 96 569 677 1148 245 2120 Fragments arranged by size: 2571 2120 2061 1299 1148 677 569 457 457 245 186 96 80 68 NaeI GCC'GGC Cuts at: 2044 2044 Size: 12034 NarI GG'CG_CC Cuts at: 7654 7654 Size: 12034 NcoI C'CATG_G Cuts at: 513 1065 2004 2067 3086 3526 7960 8329 513 Size: 552 939 63 1019 440 4434 369 4218 Fragments arranged by size: 4434 4218 1019 939 552 440 369 63 NdeI CA'TA_TG Cuts at: 387 2698 387 Size: 2311 9723 NgoMIV G'CCGG_C Cuts at: 2042 2042 Size: 12034 NheI G'CTAG_C Cuts at: 1052 5171 9325 1052 Size: 4119 4154 3761 Fragments arranged by size: 4154 4119 3761 NotI GC'GGCC_GC Cuts at: 9481 9481 Size: 12034 NruI TCG'CGA Cuts at: 2027 2027 Size: 12034 NsiI A_TGCA'T Cuts at: 3183 5530 3183 Size: 2347 9687 NspI r_CATG'y Cuts at: 1132 1229 2740 3385 3599 5766 7284 7602 8437 Size: 97 1511 645 214 2167 1518 318 835 Cuts at: 8437 8686 9325 9392 10937 1132 Size: 249 639 67 1545 2229 Fragments arranged by size: 2229 2167 1545 1518 1511 835 645 639 318 249 214 97 67 PasI CC'CwG_GG Cuts at: 1348 3376 8754 1348 Size: 2028 5378 4628 Fragments arranged by size: 5378 4628 2028 PciI A'CATG_T Cuts at: 2736 10933 2736 Size: 8197 3837 PflMI CCAn_nnn'nTGG Cuts at: 1108 4452 1108 Size: 3344 8690 PfoI T'CCnGG_A Cuts at: 3093 3229 5655 8638 3093 Size: 136 2426 2983 6489 Fragments arranged by size: 6489 2983 2426 136 PmeI GTTT'AAAC Cuts at: 9338 9338 Size: 12034 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6221 6253 10103 10135 11616 11648 6221 Size: 32 3850 32 1481 32 6607 Fragments arranged by size: 6607 3850 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2930 7984 9292 2930 Size: 5054 1308 5672 Fragments arranged by size: 5672 5054 1308 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 12034 PsiI TTA'TAA Cuts at: 6699 7716 9604 6699 Size: 1017 1888 9129 Fragments arranged by size: 9129 1888 1017 PspOMI G'GGCC_C Cuts at: 2248 2248 Size: 12034 PstI C_TGCA'G Cuts at: 830 2014 2633 5010 6056 6123 7047 7748 8907 Size: 1184 619 2377 1046 67 924 701 1159 Cuts at: 8907 9386 830 Size: 479 3478 Fragments arranged by size: 3478 2377 1184 1159 1046 924 701 619 479 67 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 12034 PvuII CAG'CTG Cuts at: 2143 3442 3628 5689 8260 8717 8785 2143 Size: 1299 186 2061 2571 457 68 5392 Fragments arranged by size: 5392 2571 2061 1299 457 186 68 SacI G_AGCT'C Cuts at: 8358 8358 Size: 12034 SacII CC_GC'GG Cuts at: 2064 2064 Size: 12034 SalI G'TCGA_C Cuts at: 1936 5124 7977 9376 1936 Size: 3188 2853 1399 4594 Fragments arranged by size: 4594 3188 2853 1399 SapI GCTCTTCn'nnn_ Cuts at: 3647 3741 6728 3647 Size: 94 2987 8953 Fragments arranged by size: 8953 2987 94 SbfI CC_TGCA'GG Cuts at: 9386 9386 Size: 12034 ScaI AGT'ACT Cuts at: 2565 6185 10282 11994 2565 Size: 3620 4097 1712 2605 Fragments arranged by size: 4097 3620 2605 1712 SexAI A'CCwGG_T Cuts at: 5562 5562 Size: 12034 SfcI C'TryA_G Cuts at: 826 1045 2010 2401 2629 3200 3417 3996 4371 Size: 219 965 391 228 571 217 579 375 Cuts at: 4371 5006 5517 5580 5892 6052 6119 6833 6994 Size: 635 511 63 312 160 67 714 161 Cuts at: 6994 7043 7744 8420 8903 9382 10517 11198 11389 Size: 49 701 676 483 479 1135 681 191 Cuts at: 11389 826 Size: 1471 Fragments arranged by size: 1471 1135 965 714 701 681 676 635 579 571 511 483 479 391 375 312 228 219 217 191 161 160 67 63 49 SfoI GGC'GCC Cuts at: 7655 7655 Size: 12034 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 12034 SgrAI Cr'CCGG_yG Cuts at: 1700 2042 1700 Size: 342 11692 SmaI CCC'GGG Cuts at: 1872 2135 9361 1872 Size: 263 7226 4545 Fragments arranged by size: 7226 4545 263 SmlI C'TyrA_G Cuts at: 820 1017 1943 2799 3495 4157 4274 5801 6211 Size: 197 926 856 696 662 117 1527 410 Cuts at: 6211 6554 7826 8135 8768 8801 10138 11039 11301 Size: 343 1272 309 633 33 1337 901 262 Cuts at: 11301 820 Size: 1553 Fragments arranged by size: 1553 1527 1337 1272 926 901 856 696 662 633 410 343 309 262 197 117 33 SnaBI TAC'GTA Cuts at: 493 9825 493 Size: 9332 2702 SpeI A'CTAG_T Cuts at: 152 7487 152 Size: 7335 4699 SphI G_CATG'C Cuts at: 1132 3385 9392 1132 Size: 2253 6007 3774 Fragments arranged by size: 6007 3774 2253 SspI AAT'ATT Cuts at: 5 52 9958 5 Size: 47 9906 2081 Fragments arranged by size: 9906 2081 47 StuI AGG'CCT Cuts at: 1517 4179 8114 9023 1517 Size: 2662 3935 909 4528 Fragments arranged by size: 4528 3935 2662 909 StyI C'CwwG_G Cuts at: 513 1065 2004 2067 3086 3397 3526 3977 5099 Size: 552 939 63 1019 311 129 451 1122 Cuts at: 5099 7335 7374 7731 7960 8007 8329 8844 9473 Size: 2236 39 357 229 47 322 515 629 Cuts at: 9473 513 Size: 3074 Fragments arranged by size: 3074 2236 1122 1019 939 629 552 515 451 357 322 311 229 129 63 47 39 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2895 6171 6335 7305 7439 7803 8109 Size: 1255 1315 3276 164 970 134 364 306 Cuts at: 8109 10061 10078 10231 10416 10861 325 Size: 1952 17 153 185 445 1498 Fragments arranged by size: 3276 1952 1498 1315 1255 970 445 364 306 185 164 153 134 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2563 3137 4012 Size: 274 80 33 51 1443 586 574 875 Cuts at: 4012 5871 6183 10280 11992 96 Size: 1859 312 4097 1712 138 Fragments arranged by size: 4097 1859 1712 1443 875 586 574 312 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3704 3736 3837 3869 6794 6826 11994 12026 3704 Size: 32 101 32 2925 32 5168 32 3712 Fragments arranged by size: 5168 3712 2925 101 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 9182 1316 Size: 7866 4168 XbaI T'CTAG_A Cuts at: 9370 9370 Size: 12034 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3237 6253 7433 7918 3237 Size: 3016 1180 485 7353 Fragments arranged by size: 7353 3016 1180 485 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 12034 XmaI C'CCGG_G Cuts at: 1870 2133 9359 1870 Size: 263 7226 4545 Fragments arranged by size: 7226 4545 263 XmnI GAAnn'nnTTC Cuts at: 2916 10163 2916 Size: 7247 4787 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 9842 276 Size: 53 83 186 936 8308 2468 Fragments arranged by size: 8308 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BsiWI BsmBI BstZ17I FseI FspAI PacI PmlI PspXI PsrI RsrII SanDI SfiI SrfI SwaI