################################## # NO CUT ENZYMES ( 40 enzymes) # ################################## AclI AflII AseI AsnI AvrII BciVI BfrI BlnI BsaBI BsiWI Bsp1407I BsrBI BsrGI Bst1107I BstZ17I CpoI DraI HpaI MamI MfeI MluI MunI NdeI NheI NotI NruI PacI PmeI PshBI Psp1406I PvuI RsrII SapI SbfI ScaI SnaBI SpeI Sse8387I SspBI SwaI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AatII 2809 GACGTC GACGT^C AccIII 1938 TCCGGA T^CCGGA AcsI 1021 GAATTC [GA]^AATT[CT] AhdI 2926 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 6181 AGCGCT AGC^GCT ApoI 1021 GAATTC [GA]^AATT[CT] AscI 260 GGCGCGCC GG^CGCGCC Asp700I 4470 GAACTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 2926 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbrPI 1843 CACGTG CAC^GTG BclI 7803 TGATCA T^GATCA BglII 2285 AGATCT A^GATCT BsaAI 1843 CACGTG [CT]AC^GT[GA] BseAI 1938 TCCGGA T^CCGGA BsmBI 4483 GAGACG CGTCTC(1/5) BspDI 3340 ATCGAT AT^CGAT BspEI 1938 TCCGGA T^CCGGA BspHI 6479 TCATGA T^CATGA BssHII 261 GCGCGC G^CGCGC BssSI 607 CTCGTG CACGAG(-5/-1) ClaI 3340 ATCGAT AT^CGAT DrdI 6781 GACCTCTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 2926 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 6181 AGCGCT AGC^GCT EcoRI 1021 GAATTC G^AATTC FbaI 7803 TGATCA T^GATCA FseI 8180 GGCCGGCC GGCCGG^CC HindIII 6089 AAGCTT A^AGCTT MroI 1938 TCCGGA T^CCGGA PaeR7I 5842 CTCGAG C^TCGAG PmaCI 1843 CACGTG CAC^GTG PmlI 1843 CACGTG CAC^GTG RcaI 6479 TCATGA T^CATGA SalI 2299 GTCGAC G^TCGAC SexAI 8084 ACCAGGT A^CC[AT]GGT SgrAI 3988 CACCGGTG C[GA]^CCGG[CT]G SspI 1195 AATATT AAT^ATT Tsp509I 1022 AATT ^AATT XhoI 5842 CTCGAG C^TCGAG XmnI 4470 GAACTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AccI 1348 GTAGAC GT^[AC][GT]AC 2299 GTCGAC AgeI 3530 ACCGGT A^CCGGT 3989 ACCGGT AspI 1384 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC 4732 GACTGCGTC AviII 1847 TGCGCA TGC^GCA 4224 TGCGCA BbvCI 5799 CCTCAGC CCTCAGC(-5/-2) 7075 GCTGAGG BstBI 3949 TTCGAA TT^CGAA 6442 TTCGAA BstEII 2781 GGTCACC G^GT[ACGT]ACC 3652 GGTCACC BstPI 2781 GGTCACC G^GT[ACGT]ACC 3652 GGTCACC EcoO65I 2781 GGTCACC G^GT[ACGT]ACC 3652 GGTCACC EcoRV 3082 GATATC GAT^ATC 5905 GATATC EcoT22I 3972 ATGCAT ATGCA^T 8254 ATGCAT FspI 1847 TGCGCA TGC^GCA 4224 TGCGCA MseI 3512 TTAA T^TAA 4382 TTAA NsiI 3972 ATGCAT ATGCA^T 8254 ATGCAT NspV 3949 TTCGAA TT^CGAA 6442 TTCGAA PflFI 1384 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC 4732 GACTGCGTC PinAI 3530 ACCGGT A^CCGGT 3989 ACCGGT PshAI 2586 GACGGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 3088 GACGAGTGTC SfuI 3949 TTCGAA TT^CGAA 6442 TTCGAA Tru9I 3512 TTAA T^TAA 4382 TTAA Tth111I 1384 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC 4732 GACTGCGTC XbaI 2412 TCTAGA T^CTAGA 8196 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 156 enzymes) # ######################################### Acc65I AccII AciI AcyI AfaI AflIII AluI Alw44I AlwI AlwNI AocI ApaI ApaLI Asp718I AspHI AvaI AvaII BalI BamHI BanI BanII BbeI BbsI BbvI BcgI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspLU11I BspMI BsrDI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EagI EarI EclXI Eco52I Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KasI KpnI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SfiI SfoI SmaI SmlI SphI StuI StyI TaqI TfiI TseI Tsp45I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI