Restriction map for
pFN21AA1776
(Linear) (Six-base) MAPSORT of: KIAA1776.seq Check: 6603 from: 1 to: 8427
KIAA1776.orf
With 182 enzymes: SgfI *
July 18, 2007 13:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 70 394 956 1864 2134 2371 2440 2605
Size: 70 324 562 908 270 237 69 165
Cuts at: 2605 4867 5676 7414 7741 8427
Size: 2262 809 1738 327 686
Fragments arranged by size:
2262 1738 908 809 686 562 327 324
270 237 165 70 69
AatII G_ACGT'C
Cuts at: 0 2813 8427
Size: 2813 5614
AccI GT'mk_AC
Cuts at: 0 1349 2300 8427
Size: 1349 951 6127
Fragments arranged by size:
6127 1349 951
Acc65I G'GTAC_C
Cuts at: 0 2401 2998 4151 6688 8427
Size: 2401 597 1153 2537 1739
Fragments arranged by size:
2537 2401 1739 1153 597
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2458 2461 7944 8030 8425 8427
Size: 2458 3 5483 86 395 2
Fragments arranged by size:
5483 2458 395 86 3 2
AfeI AGC'GCT
Cuts at: 0 6183 8427
Size: 6183 2244
AflIII A'CryG_T
Cuts at: 0 129 3703 4742 4978 5483 8427
Size: 129 3574 1039 236 505 2944
Fragments arranged by size:
3574 2944 1039 505 236 129
AgeI A'CCGG_T
Cuts at: 0 3530 3989 8427
Size: 3530 459 4438
Fragments arranged by size:
4438 3530 459
AhdI GACnn_n'nnGTC
Cuts at: 0 2931 8427
Size: 2931 5496
AleI CACnn'nnGTG
Cuts at: 0 1379 1843 2170 3869 4787 6404 7118 8264
Size: 1379 464 327 1699 918 1617 714 1146
Cuts at: 8264 8427
Size: 163
Fragments arranged by size:
1699 1617 1379 1146 918 714 464 327
163
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3658 5832 8427
Size: 3658 2174 2595
Fragments arranged by size:
3658 2595 2174
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2735 2767 8427
Size: 2735 32 5660
Fragments arranged by size:
5660 2735 32
AlwNI CAG_nnn'CTG
Cuts at: 0 124 971 1032 1047 1370 1794 1856 2004
Size: 124 847 61 15 323 424 62 148
Cuts at: 2004 5103 5529 7328 8427
Size: 3099 426 1799 1099
Fragments arranged by size:
3099 1799 1099 847 426 424 323 148
124 62 61 15
ApaI G_GGCC'C
Cuts at: 0 34 491 1183 6896 7001 7604 7692 8427
Size: 34 457 692 5713 105 603 88 735
Fragments arranged by size:
5713 735 692 603 457 105 88 34
ApaLI G'TGCA_C
Cuts at: 0 300 612 3502 5370 6567 8427
Size: 300 312 2890 1868 1197 1860
Fragments arranged by size:
2890 1868 1860 1197 312 300
ApoI r'AATT_y
Cuts at: 0 1021 8427
Size: 1021 7406
AscI GG'CGCG_CC
Cuts at: 0 261 8427
Size: 261 8166
AvaI C'yCGr_G
Cuts at: 0 577 1007 1425 2512 2567 2628 2869 4460
Size: 577 430 418 1087 55 61 241 1591
Cuts at: 4460 4544 4638 5121 5375 5842 6242 6659 7295
Size: 84 94 483 254 467 400 417 636
Cuts at: 7295 7515 7813 8048 8427
Size: 220 298 235 379
Fragments arranged by size:
1591 1087 636 577 483 467 430 418
417 400 379 298 254 241 235 220
94 84 61 55
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 4143 4176 8427
Size: 4143 33 4251
Fragments arranged by size:
4251 4143 33
BamHI G'GATC_C
Cuts at: 0 1099 1166 2064 2865 6313 8207 8427
Size: 1099 67 898 801 3448 1894 220
Fragments arranged by size:
3448 1894 1099 898 801 220 67
BanI G'GyrC_C
Cuts at: 0 382 427 584 877 1402 1689 2146 2216
Size: 382 45 157 293 525 287 457 70
Cuts at: 2216 2401 2428 2998 3124 3400 3920 4151 4855
Size: 185 27 570 126 276 520 231 704
Cuts at: 4855 5947 6017 6081 6688 7027 7396 7489 8427
Size: 1092 70 64 607 339 369 93 938
Fragments arranged by size:
1092 938 704 607 570 525 520 457
382 369 339 293 287 276 231 185
157 126 93 70 70 64 45 27
BanII G_rGCy'C
Cuts at: 0 34 491 805 1015 1183 1565 1777 1882
Size: 34 457 314 210 168 382 212 105
Cuts at: 1882 1961 2533 5759 5851 5930 6200 6559 6896
Size: 79 572 3226 92 79 270 359 337
Cuts at: 6896 7001 7013 7604 7692 8177 8427
Size: 105 12 591 88 485 250
Fragments arranged by size:
3226 591 572 485 457 382 359 337
314 270 250 212 210 168 105 105
92 88 79 79 34 12
BbeI G_GCGC'C
Cuts at: 0 881 1693 7400 8427
Size: 881 812 5707 1027
Fragments arranged by size:
5707 1027 881 812
BbsI GAAGACnn'nnnn_
Cuts at: 0 869 4088 4691 6254 8427
Size: 869 3219 603 1563 2173
Fragments arranged by size:
3219 2173 1563 869 603
BbvCI CC'TCA_GC
Cuts at: 0 5800 7076 8427
Size: 5800 1276 1351
Fragments arranged by size:
5800 1351 1276
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2280 2314 3066 3100 4535 4569 5889 5923
Size: 2280 34 752 34 1435 34 1320 34
Cuts at: 5923 8427
Size: 2504
Fragments arranged by size:
2504 2280 1435 1320 752 34 34 34
34
BclI T'GATC_A
Cuts at: 0 7803 8427
Size: 7803 624
BfrBI ATG'CAT
Cuts at: 0 3974 8256 8427
Size: 3974 4282 171
Fragments arranged by size:
4282 3974 171
BglI GCCn_nnn'nGGC
Cuts at: 0 61 112 572 701 779 1070 1091 1449
Size: 61 51 460 129 78 291 21 358
Cuts at: 1449 1574 2258 3251 3425 3929 4775 5249 5699
Size: 125 684 993 174 504 846 474 450
Cuts at: 5699 5747 5825 5945 5993 6209 7058 7544 7619
Size: 48 78 120 48 216 849 486 75
Cuts at: 7619 7790 8370 8427
Size: 171 580 57
Fragments arranged by size:
993 849 846 684 580 504 486 474
460 450 358 291 216 174 171 129
125 120 78 78 75 61 57 51
48 48 21
BglII A'GATC_T
Cuts at: 0 2285 8427
Size: 2285 6142
BlpI GC'TnA_GC
Cuts at: 0 1564 6773 7467 8427
Size: 1564 5209 694 960
Fragments arranged by size:
5209 1564 960 694
Bme1580I G_kGCm'C
Cuts at: 0 34 304 385 491 616 1183 1890 2187
Size: 34 270 81 106 125 567 707 297
Cuts at: 2187 2431 2568 2899 3127 3325 3506 4603 4639
Size: 244 137 331 228 198 181 1097 36
Cuts at: 4639 4771 5245 5374 6020 6086 6571 6896 7001
Size: 132 474 129 646 66 485 325 105
Cuts at: 7001 7054 7494 7502 7604 7692 7719 7940 8073
Size: 53 440 8 102 88 27 221 133
Cuts at: 8073 8427
Size: 354
Fragments arranged by size:
1097 707 646 567 485 474 440 354
331 325 297 270 244 228 221 198
181 137 133 132 129 125 106 105
102 88 81 66 53 36 34 27
8
BmgBI CAC'GTC
Cuts at: 0 130 1326 2172 3213 3501 3588 6252 8427
Size: 130 1196 846 1041 288 87 2664 2175
Fragments arranged by size:
2664 2175 1196 1041 846 288 130 87
BmrI ACTGGGnnnn_n'
Cuts at: 0 4348 7677 7686 8427
Size: 4348 3329 9 741
Fragments arranged by size:
4348 3329 741 9
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1896 8019 8427
Size: 1896 6123 408
Fragments arranged by size:
6123 1896 408
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 234 935 2899 4501 7330 8191 8209
Size: 28 206 701 1964 1602 2829 861 18
Cuts at: 8209 8344 8427
Size: 135 83
Fragments arranged by size:
2829 1964 1602 861 701 206 135 83
28 18
Bpu10I CC'TnA_GC
Cuts at: 0 992 5800 7062 7076 7563 8427
Size: 992 4808 1262 14 487 864
Fragments arranged by size:
4808 1262 992 864 487 14
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 7895 8418 8427
Size: 7895 523 9
Fragments arranged by size:
7895 523 9
BsaI GGTCTCn'nnnn_
Cuts at: 0 911 6143 6935 8427
Size: 911 5232 792 1492
Fragments arranged by size:
5232 1492 911 792
BsaAI yAC'GTr
Cuts at: 0 1845 8427
Size: 1845 6582
BsaHI Gr'CG_yC
Cuts at: 0 397 878 1690 1732 2333 2708 2810 6278
Size: 397 481 812 42 601 375 102 3468
Cuts at: 6278 6293 7397 8270 8427
Size: 15 1104 873 157
Fragments arranged by size:
3468 1104 873 812 601 481 397 375
157 102 42 15
BsaWI w'CCGG_w
Cuts at: 0 545 1938 2801 3530 3989 6824 7185 8427
Size: 545 1393 863 729 459 2835 361 1242
Fragments arranged by size:
2835 1393 1242 863 729 545 459 361
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1 31 225 255 309 339 2230 2260
Size: 1 30 194 30 54 30 1891 30
Cuts at: 2260 3976 4006 4523 4553 4810 4840 6493 6523
Size: 1716 30 517 30 257 30 1653 30
Cuts at: 6523 7930 7960 8427
Size: 1407 30 467
Fragments arranged by size:
1891 1716 1653 1407 517 467 257 194
54 30 30 30 30 30 30 30
30 30 1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 995 5938 7327 8008 8427
Size: 995 4943 1389 681 419
Fragments arranged by size:
4943 1389 995 681 419
BseYI C'CCAG_C
Cuts at: 0 95 287 328 971 1040 1171 2665 2907
Size: 95 192 41 643 69 131 1494 242
Cuts at: 2907 3798 3932 4435 6067 6143 6853 6992 7430
Size: 891 134 503 1632 76 710 139 438
Cuts at: 7430 7472 7673 7927 8286 8427
Size: 42 201 254 359 141
Fragments arranged by size:
1632 1494 891 710 643 503 438 359
254 242 201 192 141 139 134 131
95 76 69 42 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 378 662 2028 2148 2973 3060 3555 3602
Size: 378 284 1366 120 825 87 495 47
Cuts at: 3602 3618 4014 4023 4311 4367 5905 6267 6284
Size: 16 396 9 288 56 1538 362 17
Cuts at: 6284 6653 6732 7245 7305 8427
Size: 369 79 513 60 1122
Fragments arranged by size:
1538 1366 1122 825 513 495 396 378
369 362 288 284 120 87 79 60
56 47 17 16 9
BsiEI CG_ry'CG
Cuts at: 0 6798 8182 8302 8427
Size: 6798 1384 120 125
Fragments arranged by size:
6798 1384 125 120
BsiHKAI G_wGCw'C
Cuts at: 0 304 616 1565 2061 3352 3415 3439 3506
Size: 304 312 949 496 1291 63 24 67
Cuts at: 3506 3989 4796 5374 5759 5920 6079 6200 6391
Size: 483 807 578 385 161 159 121 191
Cuts at: 6391 6571 7013 7125 7468 8177 8427
Size: 180 442 112 343 709 250
Fragments arranged by size:
1291 949 807 709 578 496 483 442
385 343 312 304 250 191 180 161
159 121 112 67 63 24
BsmI GAATG_Cn'
Cuts at: 0 1360 2311 2971 3478 3853 3976 4828 5334
Size: 1360 951 660 507 375 123 852 506
Cuts at: 5334 6511 6769 7102 7351 7540 8029 8427
Size: 1177 258 333 249 189 489 398
Fragments arranged by size:
1360 1177 951 852 660 507 506 489
398 375 333 258 249 189 123
BsmBI CGTCTCn'nnnn_
Cuts at: 0 4477 8427
Size: 4477 3950
Bsp1286I G_dGCh'C
Cuts at: 0 34 304 385 491 616 805 1015 1183
Size: 34 270 81 106 125 189 210 168
Cuts at: 1183 1565 1777 1882 1890 1961 2061 2187 2431
Size: 382 212 105 8 71 100 126 244
Cuts at: 2431 2533 2568 2899 3127 3325 3352 3415 3439
Size: 102 35 331 228 198 27 63 24
Cuts at: 3439 3506 3989 4603 4639 4771 4796 5245 5374
Size: 67 483 614 36 132 25 449 129
Cuts at: 5374 5759 5851 5920 5930 6020 6079 6086 6200
Size: 385 92 69 10 90 59 7 114
Cuts at: 6200 6391 6559 6571 6896 7001 7013 7054 7125
Size: 191 168 12 325 105 12 41 71
Cuts at: 7125 7468 7494 7502 7604 7692 7719 7940 8073
Size: 343 26 8 102 88 27 221 133
Cuts at: 8073 8177 8427
Size: 104 250
Fragments arranged by size:
614 483 449 385 382 343 331 325
270 250 244 228 221 212 210 198
191 189 168 168 133 132 129 126
125 114 106 105 105 104 102 102
100 92 90 88 81 71 71 69
67 63 59 41 36 35 34 27
27 26 25 24 12 12 10 8
8 7
BspEI T'CCGG_A
Cuts at: 0 1938 8427
Size: 1938 6489
BspHI T'CATG_A
Cuts at: 0 6479 8427
Size: 6479 1948
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 70 109 394 956 1864 2134 2371 2440
Size: 70 39 285 562 908 270 237 69
Cuts at: 2440 2605 3010 4271 4867 5587 5676 5824 6496
Size: 165 405 1261 596 720 89 148 672
Cuts at: 6496 7213 7313 7336 7414 7741 8427
Size: 717 100 23 78 327 686
Fragments arranged by size:
1261 908 720 717 686 672 596 562
405 327 285 270 237 165 148 100
89 78 70 69 39 23
BsrDI GCAATG_nn'
Cuts at: 0 810 1554 4296 5703 8427
Size: 810 744 2742 1407 2724
Fragments arranged by size:
2742 2724 1407 810 744
BsrFI r'CCGG_y
Cuts at: 0 833 874 925 1415 2350 2384 2717 3424
Size: 833 41 51 490 935 34 333 707
Cuts at: 3424 3530 3989 5555 6580 6926 8054 8181 8318
Size: 106 459 1566 1025 346 1128 127 137
Cuts at: 8318 8427
Size: 109
Fragments arranged by size:
1566 1128 1025 935 833 707 490 459
346 333 137 127 109 106 51 41
34
BssHII G'CGCG_C
Cuts at: 0 261 8427
Size: 261 8166
BssSI C'ACGA_G
Cuts at: 0 607 8427
Size: 607 7820
BstAPI GCAn_nnn'nTGC
Cuts at: 0 947 1370 1619 1856 2162 4514 4931 6671
Size: 947 423 249 237 306 2352 417 1740
Cuts at: 6671 7328 8427
Size: 657 1099
Fragments arranged by size:
2352 1740 1099 947 657 423 417 306
249 237
BstBI TT'CG_AA
Cuts at: 0 3950 6443 8427
Size: 3950 2493 1984
Fragments arranged by size:
3950 2493 1984
BstEII G'GTnAC_C
Cuts at: 0 2781 3652 8427
Size: 2781 871 4775
Fragments arranged by size:
4775 2781 871
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1110 1951 3490 4443 4918 6406 8384 8427
Size: 1110 841 1539 953 475 1488 1978 43
Fragments arranged by size:
1978 1539 1488 1110 953 841 475 43
BstYI r'GATC_y
Cuts at: 0 1099 1166 2064 2285 2444 2865 5099 5180
Size: 1099 67 898 221 159 421 2234 81
Cuts at: 5180 5432 6313 8207 8427
Size: 252 881 1894 220
Fragments arranged by size:
2234 1894 1099 898 881 421 252 221
220 159 81 67
Bsu36I CC'TnA_GG
Cuts at: 0 2069 2151 2712 8427
Size: 2069 82 561 5715
Fragments arranged by size:
5715 2069 561 82
BtgI C'CryG_G
Cuts at: 0 456 699 1146 1377 1944 2030 2899 3375
Size: 456 243 447 231 567 86 869 476
Cuts at: 3375 3750 5451 5499 6213 6812 7056 7494 7569
Size: 375 1701 48 714 599 244 438 75
Cuts at: 7569 8226 8265 8383 8427
Size: 657 39 118 44
Fragments arranged by size:
1701 869 714 657 599 567 476 456
447 438 375 244 243 231 118 86
75 48 44 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2615 2772 8427
Size: 2615 157 5655
Fragments arranged by size:
5655 2615 157
BtsI GCAGTG_nn'
Cuts at: 0 366 952 1202 1410 2019 2660 3027 3323
Size: 366 586 250 208 609 641 367 296
Cuts at: 3323 3630 4135 4524 5730 5895 6951 7070 7500
Size: 307 505 389 1206 165 1056 119 430
Cuts at: 7500 8073 8427
Size: 573 354
Fragments arranged by size:
1206 1056 641 609 586 573 505 430
389 367 366 354 307 296 250 208
165 119
ClaI AT'CG_AT
Cuts at: 0 3341 8427
Size: 3341 5086
DraIII CAC_nnn'GTG
Cuts at: 0 434 1619 8088 8427
Size: 434 1185 6469 339
Fragments arranged by size:
6469 1185 434 339
DrdI GACnn_nn'nnGTC
Cuts at: 0 6787 8427
Size: 6787 1640
EaeI y'GGCC_r
Cuts at: 0 84 564 927 1062 1263 1698 1752 1821
Size: 84 480 363 135 201 435 54 69
Cuts at: 1821 2348 2937 3117 3314 3378 3683 4607 5040
Size: 527 589 180 197 64 305 924 433
Cuts at: 5040 5700 6210 6396 6795 6984 8056 8096 8179
Size: 660 510 186 399 189 1072 40 83
Cuts at: 8179 8299 8427
Size: 120 128
Fragments arranged by size:
1072 924 660 589 527 510 480 435
433 399 363 305 201 197 189 186
180 135 128 120 84 83 69 64
54 40
EagI C'GGCC_G
Cuts at: 0 6795 8179 8299 8427
Size: 6795 1384 120 128
Fragments arranged by size:
6795 1384 128 120
EarI CTCTTCn'nnn_
Cuts at: 0 1081 3596 4942 8427
Size: 1081 2515 1346 3485
Fragments arranged by size:
3485 2515 1346 1081
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 8307 8427
Size: 8307 120
EcoICRI GAG'CTC
Cuts at: 0 1563 5757 6198 7011 8175 8427
Size: 1563 4194 441 813 1164 252
Fragments arranged by size:
4194 1563 1164 813 441 252
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 234 935 2458 2461 2899 4501 7330
Size: 28 206 701 1523 3 438 1602 2829
Cuts at: 7330 7944 8030 8191 8209 8344 8425 8427
Size: 614 86 161 18 135 81 2
Fragments arranged by size:
2829 1602 1523 701 614 438 206 161
135 86 81 28 18 3 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 772 5740 6595 7309 8427
Size: 772 4968 855 714 1118
Fragments arranged by size:
4968 1118 855 772 714
EcoO109I rG'GnC_Cy
Cuts at: 0 30 31 104 121 523 553 574 654
Size: 30 1 73 17 402 30 21 80
Cuts at: 654 728 1114 1180 2694 3231 5118 5576 6892
Size: 74 386 66 1514 537 1887 458 1316
Cuts at: 6892 6893 6997 6998 7233 7325 7601 7809 8115
Size: 1 104 1 235 92 276 208 306
Cuts at: 8115 8305 8347 8427
Size: 190 42 80
Fragments arranged by size:
1887 1514 1316 537 458 402 386 306
276 235 208 190 104 92 80 80
74 73 66 42 30 30 21 17
1 1 1
EcoRI G'AATT_C
Cuts at: 0 1021 8427
Size: 1021 7406
EcoRV GAT'ATC
Cuts at: 0 3084 5907 8427
Size: 3084 2823 2520
Fragments arranged by size:
3084 2823 2520
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3795 8427
Size: 3795 4632
FseI GG_CCGG'CC
Cuts at: 0 8185 8427
Size: 8185 242
FspI TGC'GCA
Cuts at: 0 1849 4226 8427
Size: 1849 2377 4201
Fragments arranged by size:
4201 2377 1849
HaeII r_GCGC'y
Cuts at: 0 854 881 1693 6185 7400 7745 8427
Size: 854 27 812 4492 1215 345 682
Fragments arranged by size:
4492 1215 854 812 682 345 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 309 341 821 853 2605 2637 2857 2889
Size: 309 32 480 32 1752 32 220 32
Cuts at: 2889 2917 2949 3334 3366 4523 4555 4810 4842
Size: 28 32 385 32 1157 32 255 32
Cuts at: 4842 5902 5934 6373 6405 6493 6525 7450 7482
Size: 1060 32 439 32 88 32 925 32
Cuts at: 7482 8427
Size: 945
Fragments arranged by size:
1752 1157 1060 945 925 480 439 385
309 255 220 88 32 32 32 32
32 32 32 32 32 32 32 32
28
HincII GTy'rAC
Cuts at: 0 792 1392 1518 1626 2301 2658 3135 4740
Size: 792 600 126 108 675 357 477 1605
Cuts at: 4740 6303 6792 6807 7386 8427
Size: 1563 489 15 579 1041
Fragments arranged by size:
1605 1563 1041 792 675 600 579 489
477 357 126 108 15
HindIII A'AGCT_T
Cuts at: 0 6089 8427
Size: 6089 2338
KasI G'GCGC_C
Cuts at: 0 877 1689 7396 8427
Size: 877 812 5707 1031
Fragments arranged by size:
5707 1031 877 812
KpnI G_GTAC'C
Cuts at: 0 2405 3002 4155 6692 8427
Size: 2405 597 1153 2537 1735
Fragments arranged by size:
2537 2405 1735 1153 597
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 807 2320 3837 8427
Size: 807 1513 1517 4590
Fragments arranged by size:
4590 1517 1513 807
MscI TGG'CCA
Cuts at: 0 86 566 3380 3685 4609 5042 5702 6212
Size: 86 480 2814 305 924 433 660 510
Cuts at: 6212 8098 8427
Size: 1886 329
Fragments arranged by size:
2814 1886 924 660 510 480 433 329
305 86
MslI CAynn'nnrTG
Cuts at: 0 1379 1744 1843 2170 3193 3211 3640 3869
Size: 1379 365 99 327 1023 18 429 229
Cuts at: 3869 4787 4939 6404 7118 7574 8264 8427
Size: 918 152 1465 714 456 690 163
Fragments arranged by size:
1465 1379 1023 918 714 690 456 429
365 327 229 163 152 99 18
MspA1I CmG'CkG
Cuts at: 0 95 332 458 497 597 701 785 818
Size: 95 237 126 39 100 104 84 33
Cuts at: 818 908 979 1044 1053 1268 1295 1511 1784
Size: 90 71 65 9 215 27 216 273
Cuts at: 1784 1990 2117 2243 2459 2678 2901 3237 3539
Size: 206 127 126 216 219 223 336 302
Cuts at: 3539 3659 3798 3914 4037 4157 4280 4409 4439
Size: 120 139 116 123 120 123 129 30
Cuts at: 4439 5234 5363 5719 5764 5858 6113 6323 6522
Size: 795 129 356 45 94 255 210 199
Cuts at: 6522 6539 6800 6814 7004 7160 7289 7412 7529
Size: 17 261 14 190 156 129 123 117
Cuts at: 7529 7610 7649 7655 7775 7871 8228 8286 8415
Size: 81 39 6 120 96 357 58 129
Cuts at: 8415 8427
Size: 12
Fragments arranged by size:
795 357 356 336 302 273 261 255
237 223 219 216 216 215 210 206
199 190 156 139 129 129 129 129
127 126 126 123 123 123 120 120
120 117 116 104 100 96 95 94
90 84 81 71 65 58 45 39
39 33 30 27 17 14 12 9
6
NaeI GCC'GGC
Cuts at: 0 876 927 2352 2386 2719 3426 6582 8183
Size: 876 51 1425 34 333 707 3156 1601
Cuts at: 8183 8427
Size: 244
Fragments arranged by size:
3156 1601 1425 876 707 333 244 51
34
NarI GG'CG_CC
Cuts at: 0 878 1690 7397 8427
Size: 878 812 5707 1030
Fragments arranged by size:
5707 1030 878 812
NcoI C'CATG_G
Cuts at: 0 1146 3375 3750 5451 7056 7569 8383 8427
Size: 1146 2229 375 1701 1605 513 814 44
Fragments arranged by size:
2229 1701 1605 1146 814 513 375 44
NgoMIV G'CCGG_C
Cuts at: 0 874 925 2350 2384 2717 3424 6580 8181
Size: 874 51 1425 34 333 707 3156 1601
Cuts at: 8181 8427
Size: 246
Fragments arranged by size:
3156 1601 1425 874 707 333 246 51
34
NsiI A_TGCA'T
Cuts at: 0 3976 8258 8427
Size: 3976 4282 169
Fragments arranged by size:
4282 3976 169
NspI r_CATG'y
Cuts at: 0 860 1460 3581 3645 3707 4982 5487 6422
Size: 860 600 2121 64 62 1275 505 935
Cuts at: 6422 6720 8256 8427
Size: 298 1536 171
Fragments arranged by size:
2121 1536 1275 935 860 600 505 298
171 64 62
PasI CC'CwG_GG
Cuts at: 0 3298 3901 4566 4872 5965 7662 8351 8427
Size: 3298 603 665 306 1093 1697 689 76
Fragments arranged by size:
3298 1697 1093 689 665 603 306 76
PciI A'CATG_T
Cuts at: 0 3703 4978 5483 8427
Size: 3703 1275 505 2944
Fragments arranged by size:
3703 2944 1275 505
PflMI CCAn_nnn'nTGG
Cuts at: 0 741 1794 4212 7698 7830 8427
Size: 741 1053 2418 3486 132 597
Fragments arranged by size:
3486 2418 1053 741 597 132
PfoI T'CCnGG_A
Cuts at: 0 123 2447 4478 8168 8427
Size: 123 2324 2031 3690 259
Fragments arranged by size:
3690 2324 2031 259 123
PmlI CAC'GTG
Cuts at: 0 1845 8427
Size: 1845 6582
PpuMI rG'GwC_Cy
Cuts at: 0 121 523 553 1114 3231 5576 7233 7325
Size: 121 402 30 561 2117 2345 1657 92
Cuts at: 7325 8347 8427
Size: 1022 80
Fragments arranged by size:
2345 2117 1657 1022 561 402 121 92
80 30
PshAI GACnn'nnGTC
Cuts at: 0 2590 3092 8427
Size: 2590 502 5335
Fragments arranged by size:
5335 2590 502
PspOMI G'GGCC_C
Cuts at: 0 30 487 1179 6892 6997 7600 7688 8427
Size: 30 457 692 5713 105 603 88 739
Fragments arranged by size:
5713 739 692 603 457 105 88 30
PspXI vC'TCGA_Gb
Cuts at: 0 5842 8427
Size: 5842 2585
PstI C_TGCA'G
Cuts at: 0 120 3007 4408 4519 4902 5520 6124 6917
Size: 120 2887 1401 111 383 618 604 793
Cuts at: 6917 7654 8285 8420 8427
Size: 737 631 135 7
Fragments arranged by size:
2887 1401 793 737 631 618 604 383
135 120 111 7
PvuII CAG'CTG
Cuts at: 0 332 597 785 979 2243 3539 3798 3914
Size: 332 265 188 194 1264 1296 259 116
Cuts at: 3914 4409 5719 5764 7289 7655 7871 8286 8415
Size: 495 1310 45 1525 366 216 415 129
Cuts at: 8415 8427
Size: 12
Fragments arranged by size:
1525 1310 1296 1264 495 415 366 332
265 259 216 194 188 129 116 45
12
SacI G_AGCT'C
Cuts at: 0 1565 5759 6200 7013 8177 8427
Size: 1565 4194 441 813 1164 250
Fragments arranged by size:
4194 1565 1164 813 441 250
SacII CC_GC'GG
Cuts at: 0 459 702 2902 6815 8229 8427
Size: 459 243 2200 3913 1414 198
Fragments arranged by size:
3913 2200 1414 459 243 198
SalI G'TCGA_C
Cuts at: 0 2299 8427
Size: 2299 6128
SanDI GG'GwC_CC
Cuts at: 0 553 1114 3231 8427
Size: 553 561 2117 5196
Fragments arranged by size:
5196 2117 561 553
SexAI A'CCwGG_T
Cuts at: 0 8084 8427
Size: 8084 343
SfcI C'TryA_G
Cuts at: 0 116 255 2238 3003 3915 4404 4515 4898
Size: 116 139 1983 765 912 489 111 383
Cuts at: 4898 5445 5516 6120 6342 6913 7038 7650 7860
Size: 547 71 604 222 571 125 612 210
Cuts at: 7860 7866 8281 8416 8427
Size: 6 415 135 11
Fragments arranged by size:
1983 912 765 612 604 571 547 489
415 383 222 210 139 135 125 116
111 71 11 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 61 572 1070 8427
Size: 61 511 498 7357
Fragments arranged by size:
7357 511 498 61
SfoI GGC'GCC
Cuts at: 0 879 1691 7398 8427
Size: 879 812 5707 1029
Fragments arranged by size:
5707 1029 879 812
SgrAI Cr'CCGG_yG
Cuts at: 0 3989 8427
Size: 3989 4438
SmaI CCC'GGG
Cuts at: 0 1427 2569 2630 4640 5123 6661 7517 8427
Size: 1427 1142 61 2010 483 1538 856 910
Fragments arranged by size:
2010 1538 1427 1142 910 856 483 61
SmlI C'TyrA_G
Cuts at: 0 5842 7910 8397 8427
Size: 5842 2068 487 30
Fragments arranged by size:
5842 2068 487 30
SphI G_CATG'C
Cuts at: 0 1460 6720 8256 8427
Size: 1460 5260 1536 171
Fragments arranged by size:
5260 1536 1460 171
SspI AAT'ATT
Cuts at: 0 1197 8427
Size: 1197 7230
StuI AGG'CCT
Cuts at: 0 767 2857 5047 5068 5512 6132 7444 8395
Size: 767 2090 2190 21 444 620 1312 951
Cuts at: 8395 8427
Size: 32
Fragments arranged by size:
2190 2090 1312 951 767 620 444 32
21
StyI C'CwwG_G
Cuts at: 0 87 107 617 1146 2423 3317 3375 3624
Size: 87 20 510 529 1277 894 58 249
Cuts at: 3624 3750 5451 7056 7146 7542 7569 8126 8383
Size: 126 1701 1605 90 396 27 557 257
Cuts at: 8383 8427
Size: 44
Fragments arranged by size:
1701 1605 1277 894 557 529 510 396
257 249 126 90 87 58 44 27
20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1333 1855 3482 3924 4325 4508 7444 7687
Size: 1333 522 1627 442 401 183 2936 243
Cuts at: 7687 8427
Size: 740
Fragments arranged by size:
2936 1627 1333 740 522 442 401 243
183
TatI w'GTAC_w
Cuts at: 0 4622 5668 8427
Size: 4622 1046 2759
Fragments arranged by size:
4622 2759 1046
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 225 257 2574 2606 3976 4008 4224 4256
Size: 225 32 2317 32 1370 32 216 32
Cuts at: 4256 5763 5795 7930 7962 8427
Size: 1507 32 2135 32 465
Fragments arranged by size:
2317 2135 1507 1370 465 225 216 32
32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1387 4735 8427
Size: 1387 3348 3692
Fragments arranged by size:
3692 3348 1387
XbaI T'CTAG_A
Cuts at: 0 2412 8196 8427
Size: 2412 5784 231
Fragments arranged by size:
5784 2412 231
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2227 3295 4918 5104 8427
Size: 2227 1068 1623 186 3323
Fragments arranged by size:
3323 2227 1623 1068 186
XhoI C'TCGA_G
Cuts at: 0 5842 8427
Size: 5842 2585
XmaI C'CCGG_G
Cuts at: 0 1425 2567 2628 4638 5121 6659 7515 8427
Size: 1425 1142 61 2010 483 1538 856 912
Fragments arranged by size:
2010 1538 1425 1142 912 856 483 61
XmnI GAAnn'nnTTC
Cuts at: 0 4474 8427
Size: 4474 3953
ZraI GAC'GTC
Cuts at: 0 2811 8427
Size: 2811 5616
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI HaeII Hin4I HincII HindIII KasI
KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PasI PciI PflMI PfoI PmlI
PpuMI PshAI PspOMI PspXI PstI PvuII SacI SacII
SalI SanDI SexAI SfcI SfiI SfoI SgrAI SmaI
SmlI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AclI AflII AseI AsiSI AvrII BciVI BmtI BsaBI
BsiWI BsrBI BsrGI BstZ17I CspCI DraI FspAI HpaI
MfeI MluI NdeI NheI NotI NruI PacI PmeI
PpiI PsiI PsrI PvuI RsrII SapI SbfI ScaI
SgfI SnaBI SpeI SrfI SwaI