(Circular) (Six-base) MAPSORT of: pFN21AB7824.seq Check: 9830 from: 1 to: 6517 pFN21AB7824 6517 bp With 182 enzymes: SgfI * October 20, 2009 10:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4327 278 Size: 53 83 186 936 2791 2468 Fragments arranged by size: 2791 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2909 3552 3860 1937 Size: 972 643 308 4594 Fragments arranged by size: 4594 972 643 308 Acc65I G'GTAC_C Cuts at: 3838 3838 Size: 6517 AclI AA'CG_TT Cuts at: 1563 4644 5017 1563 Size: 3081 373 3063 Fragments arranged by size: 3081 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2485 2610 2887 3139 4580 5964 6232 1632 Size: 853 125 277 252 1441 1384 268 1917 Fragments arranged by size: 1917 1441 1384 853 277 268 252 125 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6320 AflIII A'CryG_T Cuts at: 1203 5416 6393 1203 Size: 4213 977 1327 Fragments arranged by size: 4213 1327 977 AgeI A'CCGG_T Cuts at: 5348 5348 Size: 6517 AhdI GACnn_n'nnGTC Cuts at: 5246 5246 Size: 6517 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6517 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6517 AlwNI CAG_nnn'CTG Cuts at: 2633 4260 5832 2633 Size: 1627 1572 3318 Fragments arranged by size: 3318 1627 1572 ApaLI G'TGCA_C Cuts at: 4574 5730 4574 Size: 1156 5361 ApoI r'AATT_y Cuts at: 1493 2276 2484 3826 4052 4204 6313 1493 Size: 783 208 1342 226 152 2109 1697 Fragments arranged by size: 2109 1697 1342 783 226 208 152 AseI AT'TA_AT Cuts at: 160 1032 5071 160 Size: 872 4039 1606 Fragments arranged by size: 4039 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6517 AvaI C'yCGr_G Cuts at: 1870 1943 2860 3842 1870 Size: 73 917 982 4545 Fragments arranged by size: 4545 982 917 73 BamHI G'GATC_C Cuts at: 3847 4214 3847 Size: 367 6150 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3264 3838 5293 618 Size: 325 217 627 1477 574 1455 1842 Fragments arranged by size: 1842 1477 1455 627 574 325 217 BanII G_rGCy'C Cuts at: 1391 3107 3836 1391 Size: 1716 729 4072 Fragments arranged by size: 4072 1716 729 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3805 928 Size: 971 1906 3640 Fragments arranged by size: 3640 1906 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3221 3255 4708 4742 1903 Size: 34 1284 34 1453 34 3678 Fragments arranged by size: 3678 1453 1284 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4409 5619 4409 Size: 1210 5307 BclI T'GATC_A Cuts at: 1548 1548 Size: 6517 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5128 136 Size: 107 122 71 631 605 3456 1525 Fragments arranged by size: 3456 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2361 6512 2361 Size: 4151 2366 BlpI GC'TnA_GC Cuts at: 3934 3934 Size: 6517 Bme1580I G_kGCm'C Cuts at: 1790 2717 3234 3305 3389 3473 4578 5734 1790 Size: 927 517 71 84 84 1105 1156 2573 Fragments arranged by size: 2573 1156 1105 927 517 84 84 71 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6517 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2516 2672 3301 Size: 416 53 83 399 24 1099 156 629 Cuts at: 3301 4248 5206 442 Size: 947 958 1753 Fragments arranged by size: 1753 1099 958 947 629 416 399 156 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6517 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2121 2121 Size: 6517 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2156 2222 2655 3077 5177 1239 Size: 917 66 433 422 2100 2579 Fragments arranged by size: 2579 2100 917 433 422 66 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3158 4642 5507 5805 3158 Size: 1484 865 298 3870 Fragments arranged by size: 3870 1484 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2161 5180 882 Size: 617 662 3019 2219 Fragments arranged by size: 3019 2219 662 617 BsaAI yAC'GTr Cuts at: 493 1204 2648 3550 4308 493 Size: 711 1444 902 758 2702 Fragments arranged by size: 2702 1444 902 758 711 BsaBI GATnn'nnATC Cuts at: 6511 6511 Size: 6517 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2812 4324 4706 5341 Size: 53 83 186 936 1279 1512 382 635 Cuts at: 5341 275 Size: 1451 Fragments arranged by size: 1512 1451 1279 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4950 5348 5622 5769 6180 4950 Size: 398 274 147 411 5287 Fragments arranged by size: 5287 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2001 2031 2858 2888 3698 Size: 30 165 30 598 30 827 30 810 Cuts at: 3698 3728 1178 Size: 30 3967 Fragments arranged by size: 3967 827 810 598 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2243 2315 3047 3080 3161 3497 3551 3554 Size: 1057 72 732 33 81 336 54 3 Cuts at: 3554 1186 Size: 4149 Fragments arranged by size: 4149 1057 732 336 81 72 54 33 3 BseYI C'CCAG_C Cuts at: 5720 6216 5720 Size: 496 6021 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2902 1163 Size: 573 1166 4778 Fragments arranged by size: 4778 1166 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3967 4728 5756 1540 Size: 275 186 1966 761 1028 2301 Fragments arranged by size: 2301 1966 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3107 4578 4663 5734 3107 Size: 1471 85 1071 3890 Fragments arranged by size: 3890 1471 1071 85 BsmI GAATG_Cn' Cuts at: 2726 4028 4121 2726 Size: 1302 93 5122 Fragments arranged by size: 5122 1302 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2717 3107 3234 3305 3389 3473 3836 Size: 399 927 390 127 71 84 84 363 Cuts at: 3836 4578 4663 5734 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 927 742 399 390 363 127 85 84 84 71 BspEI T'CCGG_A Cuts at: 6180 6180 Size: 6517 BspHI T'CATG_A Cuts at: 4406 6136 4406 Size: 1730 4787 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3872 844 Size: 3028 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4404 1394 Size: 429 2581 3507 Fragments arranged by size: 3507 2581 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5012 5186 65 Size: 1183 3764 174 1396 Fragments arranged by size: 3764 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5161 5348 1700 Size: 110 3351 187 2869 Fragments arranged by size: 3351 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2118 96 Size: 2022 4495 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6517 BssSI C'ACGA_G Cuts at: 4577 5589 4577 Size: 1012 5505 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6517 BstBI TT'CG_AA Cuts at: 5321 5321 Size: 6517 BstEII G'GTnAC_C Cuts at: 1184 2516 3334 3418 1184 Size: 1332 818 84 4283 Fragments arranged by size: 4283 1332 818 84 BstXI CCAn_nnnn'nTGG Cuts at: 2529 2529 Size: 6517 BstYI r'GATC_y Cuts at: 1972 2361 3847 4214 4599 4616 6068 6154 6166 Size: 389 1486 367 385 17 1452 86 12 Cuts at: 6166 6512 1972 Size: 346 1977 Fragments arranged by size: 1977 1486 1452 389 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2910 2910 Size: 6517 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5965 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3654 487 Size: 683 56 2428 3350 Fragments arranged by size: 3350 2428 683 56 BtsI GCAGTG_nn' Cuts at: 4033 4827 4847 4033 Size: 794 20 5703 Fragments arranged by size: 5703 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6517 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6482 DraI TTT'AAA Cuts at: 3821 3821 Size: 6517 DraIII CAC_nnn'GTG Cuts at: 2302 2302 Size: 6517 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5524 809 Size: 507 4208 1802 Fragments arranged by size: 4208 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2347 3577 3964 Size: 54 1425 50 275 18 517 1230 387 Cuts at: 3964 4853 6482 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1230 889 517 387 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3964 1537 Size: 275 2152 4090 Fragments arranged by size: 4090 2152 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4447 1353 Size: 3094 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5104 5478 5624 387 Size: 1064 138 3515 374 146 1280 Fragments arranged by size: 3515 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3105 3105 Size: 6517 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2156 2222 2485 2610 2655 2887 3077 Size: 393 524 66 263 125 45 232 190 Cuts at: 3077 3139 4580 5177 5964 6232 1239 Size: 62 1441 597 787 268 1524 Fragments arranged by size: 1524 1441 787 597 524 393 268 263 232 190 125 66 62 45 EcoO109I rG'GnC_Cy Cuts at: 2112 2450 2955 3123 2112 Size: 338 505 168 5506 Fragments arranged by size: 5506 505 338 168 EcoRI G'AATT_C Cuts at: 2276 3826 4204 2276 Size: 1550 378 4589 Fragments arranged by size: 4589 1550 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3775 3775 Size: 6517 FspI TGC'GCA Cuts at: 5023 5023 Size: 6517 HaeII r_GCGC'y Cuts at: 1678 5664 1678 Size: 3986 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5158 5190 5232 5264 1373 Size: 32 135 32 3586 32 42 32 2626 Fragments arranged by size: 3586 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3861 4107 669 Size: 988 281 1923 246 3079 Fragments arranged by size: 3079 1923 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6517 HpaI GTT'AAC Cuts at: 4107 4107 Size: 6517 KpnI G_GTAC'C Cuts at: 3842 3842 Size: 6517 MfeI C'AATT_G Cuts at: 4116 4116 Size: 6517 MluI A'CGCG_T Cuts at: 6393 6393 Size: 6517 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2419 3706 5631 5815 1305 Size: 1114 1287 1925 184 2007 Fragments arranged by size: 2007 1925 1287 1114 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2349 3579 10 Size: 54 1425 343 517 1230 2948 Fragments arranged by size: 2948 1425 1230 517 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2514 2794 4475 4834 4993 518 Size: 648 1348 280 1681 359 159 2042 Fragments arranged by size: 2042 1681 1348 648 359 280 159 MspA1I CmG'CkG Cuts at: 1606 2200 3691 3933 4610 5758 6003 1606 Size: 594 1491 242 677 1148 245 2120 Fragments arranged by size: 2120 1491 1148 677 594 245 242 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5965 NdeI CA'TA_TG Cuts at: 387 387 Size: 6517 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6517 NotI GC'GGCC_GC Cuts at: 3964 3964 Size: 6517 NspI r_CATG'y Cuts at: 1132 1229 3875 5420 1132 Size: 97 2646 1545 2229 Fragments arranged by size: 2646 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2161 1348 Size: 813 5704 PciI A'CATG_T Cuts at: 5416 5416 Size: 6517 PflMI CCAn_nnn'nTGG Cuts at: 1108 2302 1108 Size: 1194 5323 PfoI T'CCnGG_A Cuts at: 2052 2052 Size: 6517 PmeI GTTT'AAAC Cuts at: 3821 3821 Size: 6517 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4586 4618 6099 6131 4586 Size: 32 1481 32 4972 Fragments arranged by size: 4972 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2112 2112 Size: 6517 PshAI GACnn'nnGTC Cuts at: 1613 2253 1613 Size: 640 5877 PsiI TTA'TAA Cuts at: 4087 4087 Size: 6517 PstI C_TGCA'G Cuts at: 830 2383 3725 3869 830 Size: 1553 1342 144 3478 Fragments arranged by size: 3478 1553 1342 144 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6517 PvuII CAG'CTG Cuts at: 2200 2200 Size: 6517 SacI G_AGCT'C Cuts at: 3107 3107 Size: 6517 SalI G'TCGA_C Cuts at: 1936 3859 1936 Size: 1923 4594 SbfI CC_TGCA'GG Cuts at: 3869 3869 Size: 6517 ScaI AGT'ACT Cuts at: 4765 6477 4765 Size: 1712 4805 SexAI A'CCwGG_T Cuts at: 2298 3183 2298 Size: 885 5632 SfcI C'TryA_G Cuts at: 826 1045 2143 2379 2656 3721 3865 5000 5681 Size: 219 1098 236 277 1065 144 1135 681 Cuts at: 5681 5872 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1098 1065 681 277 236 219 191 144 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6517 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6517 SmaI CCC'GGG Cuts at: 1872 3844 1872 Size: 1972 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3137 4621 5522 5784 820 Size: 197 926 1194 1484 901 262 1553 Fragments arranged by size: 1553 1484 1194 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3550 4308 493 Size: 3057 758 2702 Fragments arranged by size: 3057 2702 758 SpeI A'CTAG_T Cuts at: 152 152 Size: 6517 SphI G_CATG'C Cuts at: 1132 3875 1132 Size: 2743 3774 SspI AAT'ATT Cuts at: 5 52 4441 5 Size: 47 4389 2081 Fragments arranged by size: 4389 2081 47 StuI AGG'CCT Cuts at: 1517 2831 1517 Size: 1314 5203 StyI C'CwwG_G Cuts at: 513 1065 2219 3956 513 Size: 552 1154 1737 3074 Fragments arranged by size: 3074 1737 1154 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2090 4544 4561 4714 4899 5344 325 Size: 1255 510 2454 17 153 185 445 1498 Fragments arranged by size: 2454 1498 1255 510 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2118 4763 6475 Size: 274 80 33 51 1443 141 2645 1712 Cuts at: 6475 96 Size: 138 Fragments arranged by size: 2645 1712 1443 274 141 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2001 2033 2349 2381 6477 6509 2001 Size: 32 316 32 4096 32 2009 Fragments arranged by size: 4096 2009 316 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3781 1316 Size: 2465 4052 XbaI T'CTAG_A Cuts at: 3853 3853 Size: 6517 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6517 XmaI C'CCGG_G Cuts at: 1870 3842 1870 Size: 1972 4545 XmnI GAAnn'nnTTC Cuts at: 2241 3041 4646 2241 Size: 800 1605 4112 Fragments arranged by size: 4112 1605 800 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4325 276 Size: 53 83 186 936 2791 2468 Fragments arranged by size: 2791 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI Bsu36I EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI