Restriction map for
pFN21AA1555
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# NO CUT ENZYMES ( 55 enzymes) #
##################################
AccI AccIII AgeI AhdI Aor51HI
AscI AseI AsnI AspEI AspI
AviII AvrII BciVI BclI BlnI
BseAI BsiWI BsmBI BspEI BspHI
BssHII Bst1107I BstZ17I DrdI Eam1105I
Eco47III EcoRV FbaI FseI FspI
HpaI MfeI MluI MroI MunI
NheI NotI NruI PacI PflFI
PinAI PmeI PshBI PvuI RcaI
SalI SbfI SexAI SgrAI SnaBI
Sse8387I SspI SwaI Tth111I XbaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AatII 5332 GACGTC GACGT^C
Acc65I 6169 GGTACC G^GTACC
AclI 2992 AACGTT AA^CGTT
AflII 1101 CTTAAG C^TTAAG
Alw44I 7004 GTGCAC G^TGCAC
ApaLI 7004 GTGCAC G^TGCAC
Asp700I 6519 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 6169 GGTACC G^GTACC
BalI 4991 TGGCCA TGG^CCA
BbeI 5640 GGCGCC GGCGC^C
BfrI 1101 CTTAAG C^TTAAG
BsaWI 2344 TCCGGT [AT]^CCGG[AT]
BsmI 307 GCATTC GAATGC(1/-1)
BspDI 1123 ATCGAT AT^CGAT
BstBI 5197 TTCGAA TT^CGAA
ClaI 1123 ATCGAT AT^CGAT
CpoI 1282 CGGACCG CG^G[AT]CCG
DraIII 3281 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoT22I 2095 ATGCAT ATGCA^T
HindIII 4586 AAGCTT A^AGCTT
KasI 5640 GGCGCC G^GCGCC
KpnI 6169 GGTACC GGTAC^C
KspI 5244 CCGCGG CCGC^GG
MluNI 4991 TGGCCA TGG^CCA
MscI 4991 TGGCCA TGG^CCA
NarI 5640 GGCGCC GG^CGCC
NdeI 1938 CATATG CA^TATG
NsiI 2095 ATGCAT ATGCA^T
NspV 5197 TTCGAA TT^CGAA
PshAI 5900 GACCGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2992 AACGTT AA^CGTT
RsrII 1282 CGGACCG CG^G[AT]CCG
SacII 5244 CCGCGG CCGC^GG
SfiI 6699 GGCCCGGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 5640 GGCGCC GGC^GCC
SfuI 5197 TTCGAA TT^CGAA
SpeI 862 ACTAGT A^CTAGT
XmnI 6519 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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BbrPI 1732 CACGTG CAC^GTG
6040 CACGTG
BglII 4019 AGATCT A^GATCT
5100 AGATCT
BsaAI 1732 CACGTG [CT]AC^GT[GA]
6040 CACGTG
BsaBI 5187 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
6579 GATTCCCATC
Bsp1407I 4001 TGTACA T^GTACA
4901 TGTACA
BspLU11I 2921 ACATGT A^CATGT
6147 ACATGT
BsrBI 2832 CCGCTC CCGCTC(-3/-3)
796 GAGCGG
BsrDI 2807 GCAATG GCAATG(2/0)
3997 GCAATG
BsrGI 4001 TGTACA T^GTACA
4901 TGTACA
BssSI 5738 CACGAG CACGAG(-5/-1)
3451 CTCGTG
BstAPI 3447 GCATCTCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5420 GCAAAAAGTGC
BstEII 877 GGTCACC G^GT[ACGT]ACC
4026 GGTCACC
BstPI 877 GGTCACC G^GT[ACGT]ACC
4026 GGTCACC
DraI 4824 TTTAAA TTT^AAA
5371 TTTAAA
EcoO65I 877 GGTCACC G^GT[ACGT]ACC
4026 GGTCACC
EcoRI 3481 GAATTC G^AATTC
5279 GAATTC
MamI 5187 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
6579 GATTCCCATC
PmaCI 1732 CACGTG CAC^GTG
6040 CACGTG
PmlI 1732 CACGTG CAC^GTG
6040 CACGTG
ScaI 584 AGTACT AGT^ACT
1162 AGTACT
SspBI 4001 TGTACA T^GTACA
4901 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 144 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApaI ApoI AspHI AvaI AvaII
BamHI BanI BanII BbsI BbvCI
BbvI BcgI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco52I Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PaeR7I PflMI PleI PpuMI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI SphI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoI XhoII XmaCI XmaI