Restriction map for
pFN21AA1503
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# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
AgeI AocI Aor51HI AscI AseI
AsnI Asp700I Asp718I AviII AvrII
BamHI BbrPI BcgI BfrI BlnI
BsaBI BsiEI BsiWI BsmBI BspDI
BspLU11I BsrBI BsrFI BssSI Bst1107I
BstEII BstPI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI EagI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV FseI FspI HindIII HpaI
KpnI KspI MamI MfeI MluI
MunI NaeI NcoI NdeI NgoMIV
NheI NotI NruI PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SgrAI SnaBI SpeI Sse8387I SspI
StuI SwaI XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 415 GTCTAC GT^[AC][GT]AC
AccIII 1633 TCCGGA T^CCGGA
AhdI 1806 GACATCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 492 GGGCCC GGGCC^C
AspEI 1806 GACATCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1675 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 945 GGCGCC GGCGC^C
BbsI 1363 GAAGAC GAAGAC(2/6)
BbvCI 1427 CCTCAGC CCTCAGC(-5/-2)
BglII 545 AGATCT A^GATCT
BlpI 142 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 142 GCTGAGC GC^T[ACGT]AGC
BpuAI 1363 GAAGAC GAAGAC(2/6)
BsaAI 1618 TACGTG [CT]AC^GT[GA]
BsaWI 1633 TCCGGA [AT]^CCGG[AT]
BseAI 1633 TCCGGA T^CCGGA
Bsp1407I 393 TGTACA T^GTACA
BspEI 1633 TCCGGA T^CCGGA
BspMI 1687 ACCTGC ACCTGC(4/8)
BsrDI 1850 CATTGC GCAATG(2/0)
BsrGI 393 TGTACA T^GTACA
BssHII 1642 GCGCGC G^CGCGC
BstBI 1784 TTCGAA TT^CGAA
BstXI 2176 CCAGCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 142 GCTGAGC GC^T[ACGT]AGC
DrdI 1008 GACCTTTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 169 CCACGG C^C[GA][CT]GG
Eam1105I 1806 GACATCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1481 CCTTCCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 852 ATGCAT ATGCA^T
HaeII 945 GGCGCC [GA]GCGC^[CT]
HgaI 1665 GCGTC GACGC(5/10)
KasI 945 GGCGCC G^GCGCC
MaeIII 1175 GTGAC ^GT[ACGT]AC
MroI 1633 TCCGGA T^CCGGA
MslI 681 CATGAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 945 GGCGCC GG^CGCC
NsiI 852 ATGCAT ATGCA^T
NspV 1784 TTCGAA TT^CGAA
PflFI 1675 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 880 CAGCTG CAG^CTG
SacI 145 GAGCTC GAGCT^C
SapI 1158 GCTCTTC GCTCTTC(1/4)
SfoI 945 GGCGCC GGC^GCC
SfuI 1784 TTCGAA TT^CGAA
SmaI 2350 CCCGGG CCC^GGG
SspBI 393 TGTACA T^GTACA
Tsp45I 1175 GTGAC ^GT[GC]AC
Tth111I 1675 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1769 CCATTGATGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 2350 CCCGGG C^CCGGG
XmaI 2350 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 32 enzymes) #
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AcsI 810 AAATTT [GA]^AATT[CT]
1960 GAATTT
AcyI 945 GGCGCC G[GA]^CG[CT]C
1664 GGCGTC
Alw44I 1651 GTGCAC G^TGCAC
1741 GTGCAC
AlwNI 1414 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
2255 CAGTCTCTG
ApaLI 1651 GTGCAC G^TGCAC
1741 GTGCAC
ApoI 810 AAATTT [GA]^AATT[CT]
1960 GAATTT
BalI 89 TGGCCA TGG^CCA
1600 TGGCCA
BanI 945 GGCGCC G^G[CT][GA]CC
1240 GGTGCC
BciVI 1842 GGATAC GTATCC(6/5)
1331 GTATCC
BclI 1091 TGATCA T^GATCA
1421 TGATCA
BglI 73 GCCACTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2349 GCCCGGGAGGC
BsaHI 945 GGCGCC G[GA]^CG[CT]C
1664 GGCGTC
BsaI 736 GAGACC GGTCTC(1/5)
2307 GGTCTC
BsmI 108 GAATGC GAATGC(1/-1)
1829 GCATTC
BspHI 1052 TCATGA T^CATGA
1682 TCATGA
DraIII 191 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
1459 CACGGCGTG
EcoT14I 152 CCAAGG C^C[AT][AT]GG
1061 CCAAGG
FbaI 1091 TGATCA T^GATCA
1421 TGATCA
Hin1I 945 GGCGCC G[GA]^CG[CT]C
1664 GGCGTC
HincII 1117 GTCAAC GT[CT]^[GA]AC
1891 GTCAAC
HindII 1117 GTCAAC GT[CT]^[GA]AC
1891 GTCAAC
MluNI 89 TGGCCA TGG^CCA
1600 TGGCCA
MscI 89 TGGCCA TGG^CCA
1600 TGGCCA
MseI 2099 TTAA T^TAA
2117 TTAA
MspA1I 880 CAGCTG C[AC]G^C[GT]G
1197 CCGCTG
PflMI 443 CCATCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1038 CCAGGAGTTGG
RcaI 1052 TCATGA T^CATGA
1682 TCATGA
SmlI 185 CTCAAG C^T[CT][GA]AG
2209 CTTGAG
SphI 386 GCATGC GCATG^C
1880 GCATGC
StyI 152 CCAAGG C^C[AT][AT]GG
1061 CCAAGG
Tru9I 2099 TTAA T^TAA
2117 TTAA
Van91I 443 CCATCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1038 CCAGGAGTTGG
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# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaI AvaII BanII BbvI
BcnI BfaI BmyI BpmI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MboI MboII MflI MnlI MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI TfiI TseI
Tsp509I TspRI TthHB8I XhoII