(Circular) (Six-base) MAPSORT of: pFN21AA1375.seq Check: 5894 from: 1 to: 7309 pFN21AA1375 7309 bp With 182 enzymes: SgfI * February 27, 2009 14:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4186 4186 Size: 7309 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5119 278 Size: 53 83 186 936 3583 2468 Fragments arranged by size: 3583 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4652 1937 Size: 2715 4594 Acc65I G'GTAC_C Cuts at: 4630 4630 Size: 7309 AclI AA'CG_TT Cuts at: 1563 3427 5436 5809 1563 Size: 1864 2009 373 3063 Fragments arranged by size: 3063 2009 1864 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3472 5372 6756 7024 1632 Size: 1840 1900 1384 268 1917 Fragments arranged by size: 1917 1900 1840 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2515 3946 820 Size: 197 1498 1431 4183 Fragments arranged by size: 4183 1498 1431 197 AflIII A'CryG_T Cuts at: 1203 6208 7185 1203 Size: 5005 977 1327 Fragments arranged by size: 5005 1327 977 AgeI A'CCGG_T Cuts at: 6140 6140 Size: 7309 AhdI GACnn_n'nnGTC Cuts at: 2565 6038 2565 Size: 3473 3836 AleI CACnn'nnGTG Cuts at: 1166 3014 3563 1166 Size: 1848 549 4912 Fragments arranged by size: 4912 1848 549 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2784 1184 Size: 1600 5709 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2232 2264 2232 Size: 32 7277 AlwNI CAG_nnn'CTG Cuts at: 2422 2552 3587 5052 6624 2422 Size: 130 1035 1465 1572 3107 Fragments arranged by size: 3107 1572 1465 1035 130 ApaLI G'TGCA_C Cuts at: 5366 6522 5366 Size: 1156 6153 ApoI r'AATT_y Cuts at: 1493 2246 2810 3053 3416 3733 3908 3967 4618 Size: 753 564 243 363 317 175 59 651 Cuts at: 4618 4844 4996 7105 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 753 651 564 363 317 243 226 175 152 59 AseI AT'TA_AT Cuts at: 160 1032 5863 160 Size: 872 4831 1606 Fragments arranged by size: 4831 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7309 AvaI C'yCGr_G Cuts at: 1870 1943 2198 4543 4634 1870 Size: 73 255 2345 91 4545 Fragments arranged by size: 4545 2345 255 91 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4166 4199 4496 4529 4166 Size: 33 297 33 6946 Fragments arranged by size: 6946 297 33 33 BamHI G'GATC_C Cuts at: 4639 5006 4639 Size: 367 6942 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2477 4630 6085 618 Size: 325 217 627 690 2153 1455 1842 Fragments arranged by size: 2153 1842 1455 690 627 325 217 BanII G_rGCy'C Cuts at: 1391 2142 4309 4628 1391 Size: 751 2167 319 4072 Fragments arranged by size: 4072 2167 751 319 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4106 928 Size: 971 2207 4131 Fragments arranged by size: 4131 2207 971 BbvCI CC'TCA_GC Cuts at: 4575 4575 Size: 7309 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5500 5534 1903 Size: 34 3563 34 3678 Fragments arranged by size: 3678 3563 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5201 6411 5201 Size: 1210 6099 BclI T'GATC_A Cuts at: 1548 2221 3304 1548 Size: 673 1083 5553 Fragments arranged by size: 5553 1083 673 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5920 136 Size: 107 122 71 631 605 4248 1525 Fragments arranged by size: 4248 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2268 2986 3269 7304 2268 Size: 718 283 4035 2273 Fragments arranged by size: 4035 2273 718 283 BlpI GC'TnA_GC Cuts at: 2141 4378 4726 2141 Size: 2237 348 4724 Fragments arranged by size: 4724 2237 348 Bme1580I G_kGCm'C Cuts at: 1790 2926 5370 6526 1790 Size: 1136 2444 1156 2573 Fragments arranged by size: 2573 2444 1156 1136 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7309 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2110 5040 5998 Size: 416 53 83 399 24 693 2930 958 Cuts at: 5998 442 Size: 1753 Fragments arranged by size: 2930 1753 958 693 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2400 3194 1056 Size: 1344 794 5171 Fragments arranged by size: 5171 1344 794 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4133 4133 Size: 7309 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2529 3252 3731 4212 4314 5969 1239 Size: 1290 723 479 481 102 1655 2579 Fragments arranged by size: 2579 1655 1290 723 481 479 102 Bpu10I CC'TnA_GC Cuts at: 2350 2392 3588 4575 2350 Size: 42 1196 987 5084 Fragments arranged by size: 5084 1196 987 42 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2574 3756 5434 6299 6597 2574 Size: 1182 1678 865 298 3286 Fragments arranged by size: 3286 1678 1182 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3901 5972 882 Size: 617 2402 2071 2219 Fragments arranged by size: 2402 2219 2071 617 BsaAI yAC'GTr Cuts at: 493 1204 5100 493 Size: 711 3896 2702 Fragments arranged by size: 3896 2702 711 BsaBI GATnn'nnATC Cuts at: 2991 7303 2991 Size: 4312 2997 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5116 5498 6133 275 Size: 53 83 186 936 3583 382 635 1451 Fragments arranged by size: 3583 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5742 6140 6414 6561 6972 5742 Size: 398 274 147 411 6079 Fragments arranged by size: 6079 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2313 2343 3225 3255 1178 Size: 30 165 30 910 30 882 30 5232 Fragments arranged by size: 5232 910 882 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2150 4209 4374 4377 1186 Size: 964 2059 165 3 4118 Fragments arranged by size: 4118 2059 964 165 3 BseYI C'CCAG_C Cuts at: 4260 4267 4340 4551 6512 7008 4260 Size: 7 73 211 1961 496 4561 Fragments arranged by size: 4561 1961 496 211 73 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2045 2406 3533 1163 Size: 573 309 361 1127 4939 Fragments arranged by size: 4939 1127 573 361 309 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2170 2532 4759 5520 6548 1540 Size: 275 186 169 362 2227 761 1028 2301 Fragments arranged by size: 2301 2227 1028 761 362 275 186 169 BsiHKAI G_wGCw'C Cuts at: 2142 4162 4309 5370 5455 6526 2142 Size: 2020 147 1061 85 1071 2925 Fragments arranged by size: 2925 2020 1071 1061 147 85 BsmI GAATG_Cn' Cuts at: 3601 4820 4913 3601 Size: 1219 93 5997 Fragments arranged by size: 5997 1219 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2195 2492 2195 Size: 297 7012 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2142 2926 4162 4309 4628 5370 5455 Size: 399 352 784 1236 147 319 742 85 Cuts at: 5455 6526 1391 Size: 1071 2174 Fragments arranged by size: 2174 1236 1071 784 742 399 352 319 147 85 BspEI T'CCGG_A Cuts at: 6972 6972 Size: 7309 BspHI T'CATG_A Cuts at: 2962 5198 6928 2962 Size: 2236 1730 3343 Fragments arranged by size: 3343 2236 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4186 4597 4664 844 Size: 3342 411 67 3489 Fragments arranged by size: 3489 3342 411 67 BsrBI CCG'CTC Cuts at: 1394 1823 5196 1394 Size: 429 3373 3507 Fragments arranged by size: 3507 3373 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4140 5804 5978 65 Size: 1183 2892 1664 174 1396 Fragments arranged by size: 2892 1664 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5953 6140 1700 Size: 110 4143 187 2869 Fragments arranged by size: 4143 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7309 BssHII G'CGCG_C Cuts at: 1925 2193 1925 Size: 268 7041 BssSI C'ACGA_G Cuts at: 2186 5369 6381 2186 Size: 3183 1012 3114 Fragments arranged by size: 3183 3114 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7309 BstBI TT'CG_AA Cuts at: 6113 6113 Size: 7309 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7309 BstXI CCAn_nnnn'nTGG Cuts at: 2065 3175 4459 4526 2065 Size: 1110 1284 67 4848 Fragments arranged by size: 4848 1284 1110 67 BstYI r'GATC_y Cuts at: 1972 2268 2986 2992 3269 4639 5006 5391 5408 Size: 296 718 6 277 1370 367 385 17 Cuts at: 5408 6860 6946 6958 7304 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1370 718 385 367 346 296 277 86 17 12 6 BtgI C'CryG_G Cuts at: 513 1065 2004 2532 4458 513 Size: 552 939 528 1926 3364 Fragments arranged by size: 3364 1926 939 552 528 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6570 Fragments arranged by size: 6570 683 56 BtsI GCAGTG_nn' Cuts at: 3646 4084 4825 5619 5639 3646 Size: 438 741 794 20 5316 Fragments arranged by size: 5316 794 741 438 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7309 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7274 DraI TTT'AAA Cuts at: 2607 4613 2607 Size: 2006 5303 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6316 809 Size: 507 5000 1802 Fragments arranged by size: 5000 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2055 4483 4756 Size: 54 1425 50 275 18 225 2428 273 Cuts at: 4756 5645 7274 8 Size: 889 1629 43 Fragments arranged by size: 2428 1629 1425 889 275 273 225 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4756 1537 Size: 275 2944 4090 Fragments arranged by size: 4090 2944 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2100 5239 1353 Size: 747 3139 3423 Fragments arranged by size: 3423 3139 747 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2147 5896 6270 6416 387 Size: 1064 138 558 3749 374 146 1280 Fragments arranged by size: 3749 1280 1064 558 374 146 138 EcoICRI GAG'CTC Cuts at: 2140 4307 2140 Size: 2167 5142 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2529 3252 3472 3731 4212 4314 5372 Size: 393 897 723 220 259 481 102 1058 Cuts at: 5372 5969 6756 7024 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1058 897 787 723 597 481 393 268 259 220 102 EcoO109I rG'GnC_Cy Cuts at: 2559 4547 2559 Size: 1988 5321 EcoRI G'AATT_C Cuts at: 4618 4996 4618 Size: 378 6931 EcoRV GAT'ATC Cuts at: 4430 4430 Size: 7309 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2330 3029 3815 2330 Size: 699 786 5824 Fragments arranged by size: 5824 786 699 FspI TGC'GCA Cuts at: 5815 5815 Size: 7309 HaeII r_GCGC'y Cuts at: 1678 4235 6456 1678 Size: 2557 2221 2531 Fragments arranged by size: 2557 2531 2221 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2430 2462 2551 2583 3289 Size: 32 135 32 858 32 89 32 706 Cuts at: 3289 3321 3710 3742 5950 5982 6024 6056 1373 Size: 32 389 32 2208 32 42 32 2626 Fragments arranged by size: 2626 2208 858 706 389 135 89 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3139 4044 4653 4899 669 Size: 988 281 1201 905 609 246 3079 Fragments arranged by size: 3079 1201 988 905 609 281 246 HindIII A'AGCT_T Cuts at: 748 2347 748 Size: 1599 5710 HpaI GTT'AAC Cuts at: 3139 4899 3139 Size: 1760 5549 KpnI G_GTAC'C Cuts at: 4634 4634 Size: 7309 MfeI C'AATT_G Cuts at: 2380 3147 3216 4908 2380 Size: 767 69 1692 4781 Fragments arranged by size: 4781 1692 767 69 MluI A'CGCG_T Cuts at: 7185 7185 Size: 7309 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3357 4247 6423 6607 1305 Size: 2052 890 2176 184 2007 Fragments arranged by size: 2176 2052 2007 890 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2057 4485 10 Size: 54 1425 343 225 2428 2834 Fragments arranged by size: 2834 2428 1425 343 225 54 MslI CAynn'nnrTG Cuts at: 518 1166 2021 3014 3563 4457 5267 5626 5785 Size: 648 855 993 549 894 810 359 159 Cuts at: 5785 518 Size: 2042 Fragments arranged by size: 2042 993 894 855 810 648 549 359 159 MspA1I CmG'CkG Cuts at: 1606 2029 2065 2176 4725 5402 6550 6795 1606 Size: 423 36 111 2549 677 1148 245 2120 Fragments arranged by size: 2549 2120 1148 677 423 245 111 36 NcoI C'CATG_G Cuts at: 513 1065 2004 4458 513 Size: 552 939 2454 3364 Fragments arranged by size: 3364 2454 939 552 NdeI CA'TA_TG Cuts at: 387 3982 387 Size: 3595 3714 NheI G'CTAG_C Cuts at: 1052 2396 3190 1052 Size: 1344 794 5171 Fragments arranged by size: 5171 1344 794 NotI GC'GGCC_GC Cuts at: 4756 4756 Size: 7309 NspI r_CATG'y Cuts at: 1132 1229 2843 4211 4667 6212 1132 Size: 97 1614 1368 456 1545 2229 Fragments arranged by size: 2229 1614 1545 1368 456 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7309 PciI A'CATG_T Cuts at: 6208 6208 Size: 7309 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7309 PfoI T'CCnGG_A Cuts at: 4423 4525 4423 Size: 102 7207 PmeI GTTT'AAAC Cuts at: 4613 4613 Size: 7309 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5378 5410 6891 6923 5378 Size: 32 1481 32 5764 Fragments arranged by size: 5764 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2559 4547 2559 Size: 1988 5321 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7309 PsiI TTA'TAA Cuts at: 4879 4879 Size: 7309 PstI C_TGCA'G Cuts at: 830 2668 2935 4661 830 Size: 1838 267 1726 3478 Fragments arranged by size: 3478 1838 1726 267 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7309 PvuII CAG'CTG Cuts at: 2029 2029 Size: 7309 SacI G_AGCT'C Cuts at: 2142 4309 2142 Size: 2167 5142 SalI G'TCGA_C Cuts at: 1936 4651 1936 Size: 2715 4594 SanDI GG'GwC_CC Cuts at: 2559 4547 2559 Size: 1988 5321 SapI GCTCTTCn'nnn_ Cuts at: 2100 2100 Size: 7309 SbfI CC_TGCA'GG Cuts at: 4661 4661 Size: 7309 ScaI AGT'ACT Cuts at: 2120 5557 7269 2120 Size: 3437 1712 2160 Fragments arranged by size: 3437 2160 1712 SfcI C'TryA_G Cuts at: 826 1045 2664 2931 4657 5792 6473 6664 826 Size: 219 1619 267 1726 1135 681 191 1471 Fragments arranged by size: 1726 1619 1471 1135 681 267 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7309 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7309 SmaI CCC'GGG Cuts at: 1872 4545 4636 1872 Size: 2673 91 4545 Fragments arranged by size: 4545 2673 91 SmlI C'TyrA_G Cuts at: 820 1017 1943 2198 2515 2589 3771 3946 5413 Size: 197 926 255 317 74 1182 175 1467 Cuts at: 5413 6314 6576 820 Size: 901 262 1553 Fragments arranged by size: 1553 1467 1182 926 901 317 262 255 197 175 74 SnaBI TAC'GTA Cuts at: 493 5100 493 Size: 4607 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7309 SphI G_CATG'C Cuts at: 1132 2843 4667 1132 Size: 1711 1824 3774 Fragments arranged by size: 3774 1824 1711 SspI AAT'ATT Cuts at: 5 52 3889 5233 5 Size: 47 3837 1344 2081 Fragments arranged by size: 3837 2081 1344 47 StuI AGG'CCT Cuts at: 1517 4276 1517 Size: 2759 4550 StyI C'CwwG_G Cuts at: 513 1065 2004 2718 4458 4748 513 Size: 552 939 714 1740 290 3074 Fragments arranged by size: 3074 1740 939 714 552 290 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2156 5336 5353 5506 5691 6136 325 Size: 1255 576 3180 17 153 185 445 1498 Fragments arranged by size: 3180 1498 1255 576 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2118 3091 5555 Size: 274 80 33 51 1443 141 973 2464 Cuts at: 5555 7267 96 Size: 1712 138 Fragments arranged by size: 2464 1712 1443 973 274 141 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7269 7301 7269 Size: 32 7277 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7309 XbaI T'CTAG_A Cuts at: 2989 4645 2989 Size: 1656 5653 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2471 2471 Size: 7309 XhoI C'TCGA_G Cuts at: 1943 2198 1943 Size: 255 7054 XmaI C'CCGG_G Cuts at: 1870 4543 4634 1870 Size: 2673 91 4545 Fragments arranged by size: 4545 2673 91 XmnI GAAnn'nnTTC Cuts at: 5438 5438 Size: 7309 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5117 276 Size: 53 83 186 936 3583 2468 Fragments arranged by size: 3583 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BbeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI