Restriction map for
pFN21AA1207
(Circular) (Six-base) MAPSORT of: pFN21AA1207.seq Check: 7323 from: 1 to: 10888
pFN21AA1207 10888 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4651 8698 278
Size: 53 83 186 936 3115 4047 2468
Fragments arranged by size:
4047 3115 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2173 5412 8231 1937
Size: 236 3239 2819 4594
Fragments arranged by size:
4594 3239 2819 236
Acc65I G'GTAC_C
Cuts at: 8209 8209
Size: 10888
AclI AA'CG_TT
Cuts at: 1563 3497 9015 9388 1563
Size: 1934 5518 373 3063
Fragments arranged by size:
5518 3063 1934 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2288 3269 3285 4100 4320 4478 5235 7194
Size: 656 981 16 815 220 158 757 1959
Cuts at: 7194 7268 8951 10335 10603 1632
Size: 74 1683 1384 268 1917
Fragments arranged by size:
1959 1917 1683 1384 981 815 757 656
268 220 158 74 16
AflII C'TTAA_G
Cuts at: 820 1017 4528 5941 6041 820
Size: 197 3511 1413 100 5667
Fragments arranged by size:
5667 3511 1413 197 100
AflIII A'CryG_T
Cuts at: 1203 4116 9787 10764 1203
Size: 2913 5671 977 1327
Fragments arranged by size:
5671 2913 1327 977
AgeI A'CCGG_T
Cuts at: 9719 9719
Size: 10888
AhdI GACnn_n'nnGTC
Cuts at: 9617 9617
Size: 10888
AleI CACnn'nnGTG
Cuts at: 1166 2676 4241 5363 1166
Size: 1510 1565 1122 6691
Fragments arranged by size:
6691 1565 1510 1122
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2315 1184
Size: 1131 9757
AlwNI CAG_nnn'CTG
Cuts at: 5229 7213 8631 10203 5229
Size: 1984 1418 1572 5914
Fragments arranged by size:
5914 1984 1572 1418
ApaI G_GGCC'C
Cuts at: 2418 2495 2502 3910 7171 2418
Size: 77 7 1408 3261 6135
Fragments arranged by size:
6135 3261 1408 77 7
ApaLI G'TGCA_C
Cuts at: 6178 8945 10101 6178
Size: 2767 1156 6965
Fragments arranged by size:
6965 2767 1156
ApoI r'AATT_y
Cuts at: 1493 2051 2840 4047 4374 4694 5183 5678 6371
Size: 558 789 1207 327 320 489 495 693
Cuts at: 6371 6473 6861 7130 7531 7631 8197 8423 8575
Size: 102 388 269 401 100 566 226 152
Cuts at: 8575 10684 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1207 789 693 566 558 495
489 401 388 327 320 269 226 152
102 100
AseI AT'TA_AT
Cuts at: 160 1032 2243 4286 9442 160
Size: 872 1211 2043 5156 1606
Fragments arranged by size:
5156 2043 1606 1211 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10888
AvaI C'yCGr_G
Cuts at: 1870 1943 4829 6891 7163 8213 1870
Size: 73 2886 2062 272 1050 4545
Fragments arranged by size:
4545 2886 2062 1050 272 73
BamHI G'GATC_C
Cuts at: 2065 3190 8218 8585 2065
Size: 1125 5028 367 4368
Fragments arranged by size:
5028 4368 1125 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4492 5291 5754 5863 7877
Size: 325 217 627 2705 799 463 109 2014
Cuts at: 7877 8067 8209 9664 618
Size: 190 142 1455 1842
Fragments arranged by size:
2705 2014 1842 1455 799 627 463 325
217 190 142 109
BanII G_rGCy'C
Cuts at: 1391 2418 2495 2502 3672 3910 6572 6699 7171
Size: 1027 77 7 1170 238 2662 127 472
Cuts at: 7171 8207 1391
Size: 1036 4072
Fragments arranged by size:
4072 2662 1170 1036 1027 472 238 127
77 7
BbeI G_GCGC'C
Cuts at: 5867 7881 8071 5867
Size: 2014 190 8684
Fragments arranged by size:
8684 2014 190
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2472 3650 4110 6053 6309 6774 928
Size: 971 573 1178 460 1943 256 465 5042
Fragments arranged by size:
5042 1943 1178 971 573 465 460 256
BbvCI CC'TCA_GC
Cuts at: 3105 3105
Size: 10888
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 9079 9113 1903
Size: 34 50 34 7058 34 3678
Fragments arranged by size:
7058 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4029 4473 8780 9990 4029
Size: 444 4307 1210 4927
Fragments arranged by size:
4927 4307 1210 444
BclI T'GATC_A
Cuts at: 1548 5008 6030 6126 7380 7653 1548
Size: 3460 1022 96 1254 273 4783
Fragments arranged by size:
4783 3460 1254 1022 273 96
BfrBI ATG'CAT
Cuts at: 4418 4940 4418
Size: 522 10366
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2506 5300 9499
Size: 107 122 71 631 605 834 2794 4199
Cuts at: 9499 136
Size: 1525
Fragments arranged by size:
4199 2794 1525 834 631 605 122 107
71
BglII A'GATC_T
Cuts at: 7511 10883 7511
Size: 3372 7516
BlpI GC'TnA_GC
Cuts at: 2532 8305 2532
Size: 5773 5115
Bme1580I G_kGCm'C
Cuts at: 1790 2418 2495 2502 3910 5296 6182 7171 8949
Size: 628 77 7 1408 1386 886 989 1778
Cuts at: 8949 10105 1790
Size: 1156 2573
Fragments arranged by size:
2573 1778 1408 1386 1156 989 886 628
77 7
BmgBI CAC'GTC
Cuts at: 1318 2674 1318
Size: 1356 9532
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2355 3346 3450
Size: 416 53 83 399 24 938 991 104
Cuts at: 3450 3952 5753 5979 6758 6797 8619 9577 442
Size: 502 1801 226 779 39 1822 958 1753
Fragments arranged by size:
1822 1801 1753 991 958 938 779 502
416 399 226 104 83 53 39 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10888
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4899 5251 5357 6683 7011 7633 9548 1239
Size: 3660 352 106 1326 328 622 1915 2579
Fragments arranged by size:
3660 2579 1915 1326 622 352 328 106
Bpu10I CC'TnA_GC
Cuts at: 2408 3105 7221 2408
Size: 697 4116 6075
Fragments arranged by size:
6075 4116 697
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2185 3659 3686 4670 6422 6828 9013 9878 10176
Size: 1474 27 984 1752 406 2185 865 298
Cuts at: 10176 2185
Size: 2897
Fragments arranged by size:
2897 2185 1752 1474 984 865 406 298
27
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4194 7012 9551 882
Size: 617 2695 2818 2539 2219
Fragments arranged by size:
2818 2695 2539 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5559 7228 8679 493
Size: 711 4355 1669 1451 2702
Fragments arranged by size:
4355 2702 1669 1451 711
BsaBI GATnn'nnATC
Cuts at: 10882 10882
Size: 10888
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4648 5864 7878 8068
Size: 53 83 186 936 3115 1216 2014 190
Cuts at: 8068 8695 9077 9712 275
Size: 627 382 635 1451
Fragments arranged by size:
3115 2014 1451 1216 936 635 627 382
190 186 83 53
BsaWI w'CCGG_w
Cuts at: 2978 3069 7777 9321 9719 9993 10140 10551 2978
Size: 91 4708 1544 398 274 147 411 3315
Fragments arranged by size:
4708 3315 1544 411 398 274 147 91
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4532 4562 4897 4927 4987
Size: 30 165 30 3129 30 335 30 60
Cuts at: 4987 5017 6000 6030 6092 6122 1178
Size: 30 983 30 62 30 5944
Fragments arranged by size:
5944 3129 983 335 165 62 60 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2451 2982 5248 6021 6989 8126 1186
Size: 1265 531 2266 773 968 1137 3948
Fragments arranged by size:
3948 2266 1265 1137 968 773 531
BseYI C'CCAG_C
Cuts at: 2417 4716 4895 4980 5154 6602 7892 10091 10587
Size: 2299 179 85 174 1448 1290 2199 496
Cuts at: 10587 2417
Size: 2718
Fragments arranged by size:
2718 2299 2199 1448 1290 496 179 174
85
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 5286 7136 1163
Size: 573 3550 1850 4915
Fragments arranged by size:
4915 3550 1850 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4851 8338 9099 10127 1540
Size: 275 186 2850 3487 761 1028 2301
Fragments arranged by size:
3487 2850 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 6182 6699 7227 8136 8949 9034 10105 6182
Size: 517 528 909 813 85 1071 6965
Fragments arranged by size:
6965 1071 909 813 528 517 85
BsmI GAATG_Cn'
Cuts at: 4420 6665 8399 8492 4420
Size: 2245 1734 93 6816
Fragments arranged by size:
6816 2245 1734 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2995 2995
Size: 10888
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2418 2495 2502 3672 3910 5296 6182
Size: 399 628 77 7 1170 238 1386 886
Cuts at: 6182 6572 6699 7171 7227 8136 8207 8949 9034
Size: 390 127 472 56 909 71 742 85
Cuts at: 9034 10105 1391
Size: 1071 2174
Fragments arranged by size:
2174 1386 1170 1071 909 886 742 628
472 399 390 238 127 85 77 71
56 7
BspEI T'CCGG_A
Cuts at: 7777 10551 7777
Size: 2774 8114
BspHI T'CATG_A
Cuts at: 2889 6078 8777 10507 2889
Size: 3189 2699 1730 3270
Fragments arranged by size:
3270 3189 2699 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3696 4769 7380 7752 8243 844
Size: 2852 1073 2611 372 491 3489
Fragments arranged by size:
3489 2852 2611 1073 491 372
BsrBI CCG'CTC
Cuts at: 1394 1823 2412 4964 7180 8775 1394
Size: 429 589 2552 2216 1595 3507
Fragments arranged by size:
3507 2552 2216 1595 589 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3060 3172 7480 9383 9557 65
Size: 1183 1812 112 4308 1903 174 1396
Fragments arranged by size:
4308 1903 1812 1396 1183 174 112
BsrFI r'CCGG_y
Cuts at: 1700 1810 2568 4846 9532 9719 1700
Size: 110 758 2278 4686 187 2869
Fragments arranged by size:
4686 2869 2278 758 187 110
BsrGI T'GTAC_A
Cuts at: 96 3230 5874 6108 96
Size: 3134 2644 234 4876
Fragments arranged by size:
4876 3134 2644 234
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10888
BssSI C'ACGA_G
Cuts at: 3185 4108 8948 9960 3185
Size: 923 4840 1012 4113
Fragments arranged by size:
4840 4113 1012 923
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 10888
BstBI TT'CG_AA
Cuts at: 7636 9692 7636
Size: 2056 8832
BstEII G'GTnAC_C
Cuts at: 1184 2282 3076 1184
Size: 1098 794 8996
Fragments arranged by size:
8996 1098 794
BstXI CCAn_nnnn'nTGG
Cuts at: 2362 4716 7315 2362
Size: 2354 2599 5935
Fragments arranged by size:
5935 2599 2354
BstYI r'GATC_y
Cuts at: 1972 2065 2294 2604 3190 3612 4167 5485 6541
Size: 93 229 310 586 422 555 1318 1056
Cuts at: 6541 6579 7511 8218 8585 8970 8987 10439 10525
Size: 38 932 707 367 385 17 1452 86
Cuts at: 10525 10537 10883 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1318 1056 932 707 586 555
422 385 367 346 310 229 93 86
38 17 12
BstZ17I GTA'TAC
Cuts at: 2174 2174
Size: 10888
Bsu36I CC'TnA_GG
Cuts at: 6385 7199 6385
Size: 814 10074
BtgI C'CryG_G
Cuts at: 513 1065 5127 513
Size: 552 4062 6274
Fragments arranged by size:
6274 4062 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3944 487
Size: 683 56 2718 7431
Fragments arranged by size:
7431 2718 683 56
BtsI GCAGTG_nn'
Cuts at: 5913 6958 8404 9198 9218 5913
Size: 1045 1446 794 20 7583
Fragments arranged by size:
7583 1446 1045 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10888
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3619 3654 529
Size: 35 3055 35 7763
Fragments arranged by size:
7763 3055 35 35
DraI TTT'AAA
Cuts at: 5281 8192 5281
Size: 2911 7977
DraIII CAC_nnn'GTG
Cuts at: 5264 5264
Size: 10888
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2483 7451 9895 809
Size: 507 1167 4968 2444 1802
Fragments arranged by size:
4968 2444 1802 1167 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2680 2878 4848
Size: 54 1425 50 275 18 850 198 1970
Cuts at: 4848 6172 7853 8127 8335 9224 10853 8
Size: 1324 1681 274 208 889 1629 43
Fragments arranged by size:
1970 1681 1629 1425 1324 889 850 275
274 208 198 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4848 8335 1537
Size: 275 3036 3487 4090
Fragments arranged by size:
4090 3487 3036 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4296 4349 5324 6080 7170 7194 8126 8818
Size: 2943 53 975 756 1090 24 932 692
Cuts at: 8818 1353
Size: 3423
Fragments arranged by size:
3423 2943 1090 975 932 756 692 53
24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2370 2587 3733 5207 6172 9475
Size: 1064 138 781 217 1146 1474 965 3303
Cuts at: 9475 9849 9995 387
Size: 374 146 1280
Fragments arranged by size:
3303 1474 1280 1146 1064 965 781 374
217 146 138
EcoICRI GAG'CTC
Cuts at: 6697 6697
Size: 10888
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2288 3269 3285 4100 4320 4478 4899
Size: 393 656 981 16 815 220 158 421
Cuts at: 4899 5235 5251 5357 6683 7011 7194 7268 7633
Size: 336 16 106 1326 328 183 74 365
Cuts at: 7633 8951 9548 10335 10603 1239
Size: 1318 597 787 268 1524
Fragments arranged by size:
1524 1326 1318 981 815 787 656 597
421 393 365 336 328 268 220 183
158 106 74 16 16
EcoNI CCTnn'n_nnAGG
Cuts at: 4710 6219 4710
Size: 1509 9379
EcoO109I rG'GnC_Cy
Cuts at: 2272 2491 2492 6481 6954 7167 7168 7328 2272
Size: 219 1 3989 473 213 1 160 5832
Fragments arranged by size:
5832 3989 473 219 213 160 1 1
EcoRI G'AATT_C
Cuts at: 4374 8197 8575 4374
Size: 3823 378 6687
Fragments arranged by size:
6687 3823 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2552 5312 5494 6275 6589 7199 8011 2552
Size: 2760 182 781 314 610 812 5429
Fragments arranged by size:
5429 2760 812 781 610 314 182
FspI TGC'GCA
Cuts at: 9394 9394
Size: 10888
HaeII r_GCGC'y
Cuts at: 1678 5867 6193 7881 8071 10035 1678
Size: 4189 326 1688 190 1964 2531
Fragments arranged by size:
4189 2531 1964 1688 326 190
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2174 2206 2992 3024 3119
Size: 32 135 32 602 32 786 32 95
Cuts at: 3119 3151 3413 3445 3597 3629 4232 4264 4403
Size: 32 262 32 152 32 603 32 139
Cuts at: 4403 4435 4500 4532 4729 4761 4897 4928 4929
Size: 32 65 32 197 32 136 31 1
Cuts at: 4929 4960 5400 5432 6571 6603 7820 7852 9529
Size: 31 440 32 1139 32 1217 32 1677
Cuts at: 9529 9561 9603 9635 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1677 1217 1139 786 603 602 440
262 197 152 139 136 135 95 65
42 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 31 31 1
HincII GTy'rAC
Cuts at: 669 1657 1938 2752 4905 8232 8478 669
Size: 988 281 814 2153 3327 246 3079
Fragments arranged by size:
3327 3079 2153 988 814 281 246
HindIII A'AGCT_T
Cuts at: 748 3662 4756 748
Size: 2914 1094 6880
Fragments arranged by size:
6880 2914 1094
HpaI GTT'AAC
Cuts at: 8478 8478
Size: 10888
KasI G'GCGC_C
Cuts at: 5863 7877 8067 5863
Size: 2014 190 8684
Fragments arranged by size:
8684 2014 190
KpnI G_GTAC'C
Cuts at: 8213 8213
Size: 10888
MfeI C'AATT_G
Cuts at: 2226 8487 2226
Size: 6261 4627
MluI A'CGCG_T
Cuts at: 10764 10764
Size: 10888
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2454 2505 2836 3346 3518 4209 5859 6507
Size: 1149 51 331 510 172 691 1650 648
Cuts at: 6507 6535 7746 10002 10186 1305
Size: 28 1211 2256 184 2007
Fragments arranged by size:
2256 2007 1650 1211 1149 691 648 510
331 184 172 51 28
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2682 2880 7855 10
Size: 54 1425 343 850 198 4975 3043
Fragments arranged by size:
4975 3043 1425 850 343 198 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2400 2676 4241 4337 5363 6083 6658
Size: 648 1234 276 1565 96 1026 720 575
Cuts at: 6658 8846 9205 9364 518
Size: 2188 359 159 2042
Fragments arranged by size:
2188 2042 1565 1234 1026 720 648 575
359 276 159 96
MspA1I CmG'CkG
Cuts at: 1606 4063 4294 5270 7030 8304 8981 10129 10374
Size: 2457 231 976 1760 1274 677 1148 245
Cuts at: 10374 1606
Size: 2120
Fragments arranged by size:
2457 2120 1760 1274 1148 976 677 245
231
NaeI GCC'GGC
Cuts at: 2570 4848 2570
Size: 2278 8610
NarI GG'CG_CC
Cuts at: 5864 7878 8068 5864
Size: 2014 190 8684
Fragments arranged by size:
8684 2014 190
NcoI C'CATG_G
Cuts at: 513 1065 5127 513
Size: 552 4062 6274
Fragments arranged by size:
6274 4062 552
NdeI CA'TA_TG
Cuts at: 387 5491 387
Size: 5104 5784
NgoMIV G'CCGG_C
Cuts at: 2568 4846 2568
Size: 2278 8610
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10888
NotI GC'GGCC_GC
Cuts at: 8335 8335
Size: 10888
NsiI A_TGCA'T
Cuts at: 4420 4942 4420
Size: 522 10366
NspI r_CATG'y
Cuts at: 1132 1229 2965 4321 6115 8246 9791 1132
Size: 97 1736 1356 1794 2131 1545 2229
Fragments arranged by size:
2229 2131 1794 1736 1545 1356 97
PasI CC'CwG_GG
Cuts at: 1348 2495 5887 7273 1348
Size: 1147 3392 1386 4963
Fragments arranged by size:
4963 3392 1386 1147
PciI A'CATG_T
Cuts at: 9787 9787
Size: 10888
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3500 5025 6809 1108
Size: 2392 1525 1784 5187
Fragments arranged by size:
5187 2392 1784 1525
PmeI GTTT'AAAC
Cuts at: 8192 8192
Size: 10888
PmlI CAC'GTG
Cuts at: 5559 7228 5559
Size: 1669 9219
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8957 8989 10470 10502 8957
Size: 32 1481 32 9343
Fragments arranged by size:
9343 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2272 6954 2272
Size: 4682 6206
PshAI GACnn'nnGTC
Cuts at: 1613 7722 1613
Size: 6109 4779
PsiI TTA'TAA
Cuts at: 2802 8458 2802
Size: 5656 5232
PspOMI G'GGCC_C
Cuts at: 2414 2491 2498 3906 7167 2414
Size: 77 7 1408 3261 6135
Fragments arranged by size:
6135 3261 1408 77 7
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3474 3506 3474
Size: 32 10856
PstI C_TGCA'G
Cuts at: 830 5806 8240 830
Size: 4976 2434 3478
Fragments arranged by size:
4976 3478 2434
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10888
PvuII CAG'CTG
Cuts at: 4063 4294 5270 7030 4063
Size: 231 976 1760 7921
Fragments arranged by size:
7921 1760 976 231
SacI G_AGCT'C
Cuts at: 6699 6699
Size: 10888
SalI G'TCGA_C
Cuts at: 1936 8230 1936
Size: 6294 4594
SanDI GG'GwC_CC
Cuts at: 6954 6954
Size: 10888
SapI GCTCTTCn'nnn_
Cuts at: 4296 5324 7170 7194 8126 4296
Size: 1028 1846 24 932 7058
Fragments arranged by size:
7058 1846 1028 932 24
SbfI CC_TGCA'GG
Cuts at: 8240 8240
Size: 10888
ScaI AGT'ACT
Cuts at: 6980 9136 10848 6980
Size: 2156 1712 7020
Fragments arranged by size:
7020 2156 1712
SfcI C'TryA_G
Cuts at: 826 1045 2259 3601 5618 5802 7117 8236 9371
Size: 219 1214 1342 2017 184 1315 1119 1135
Cuts at: 9371 10052 10243 826
Size: 681 191 1471
Fragments arranged by size:
2017 1471 1342 1315 1214 1135 1119 681
219 191 184
SfiI GGCCn_nnn'nGGCC
Cuts at: 2506 2506
Size: 10888
SfoI GGC'GCC
Cuts at: 5865 7879 8069 5865
Size: 2014 190 8684
Fragments arranged by size:
8684 2014 190
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10888
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10888
SmaI CCC'GGG
Cuts at: 1872 4831 6893 8215 1872
Size: 2959 2062 1322 4545
Fragments arranged by size:
4545 2959 2062 1322
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2164 3638 3665 4528 4685 5941
Size: 197 926 221 1474 27 863 157 1256
Cuts at: 5941 6041 6437 6843 8992 9893 10155 820
Size: 100 396 406 2149 901 262 1553
Fragments arranged by size:
2149 1553 1474 1256 926 901 863 406
396 262 221 197 157 100 27
SnaBI TAC'GTA
Cuts at: 493 8679 493
Size: 8186 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10888
SphI G_CATG'C
Cuts at: 1132 4321 8246 1132
Size: 3189 3925 3774
Fragments arranged by size:
3925 3774 3189
SspI AAT'ATT
Cuts at: 5 52 3156 8812 5
Size: 47 3104 5656 2081
Fragments arranged by size:
5656 3104 2081 47
StuI AGG'CCT
Cuts at: 1517 2656 5899 1517
Size: 1139 3243 6506
Fragments arranged by size:
6506 3243 1139
StyI C'CwwG_G
Cuts at: 513 1065 2197 2502 3902 5127 5296 6096 7395
Size: 552 1132 305 1400 1225 169 800 1299
Cuts at: 7395 8327 513
Size: 932 3074
Fragments arranged by size:
3074 1400 1299 1225 1132 932 800 552
305 169
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3064 4909 5644 5755 5772 6067 6786
Size: 1255 1484 1845 735 111 17 295 719
Cuts at: 6786 7282 7660 8915 8932 9085 9270 9715 325
Size: 496 378 1255 17 153 185 445 1498
Fragments arranged by size:
1845 1498 1484 1255 1255 735 719 496
445 378 295 185 153 111 17 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3230 3540 3723
Size: 274 80 33 51 1443 1253 310 183
Cuts at: 3723 3858 4395 5874 6108 6978 9134 10846 96
Size: 135 537 1479 234 870 2156 1712 138
Fragments arranged by size:
2156 1712 1479 1443 1253 870 537 310
274 234 183 138 135 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2583 2615 4718 4750 4987 5019 6000 6032 7056
Size: 32 2103 32 237 32 981 32 1024
Cuts at: 7056 7088 7720 7752 10848 10880 2583
Size: 32 632 32 3096 32 2591
Fragments arranged by size:
3096 2591 2103 1024 981 632 237 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2521 5408 6236 6647 1316
Size: 1205 2887 828 411 5557
Fragments arranged by size:
5557 2887 1205 828 411
XbaI T'CTAG_A
Cuts at: 8224 8224
Size: 10888
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2359 4923 5455 5638 5992 6149 6190 6316 6713
Size: 2564 532 183 354 157 41 126 397
Cuts at: 6713 2359
Size: 6534
Fragments arranged by size:
6534 2564 532 397 354 183 157 126
41
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 10888
XmaI C'CCGG_G
Cuts at: 1870 4829 6891 8213 1870
Size: 2959 2062 1322 4545
Fragments arranged by size:
4545 2959 2062 1322
XmnI GAAnn'nnTTC
Cuts at: 4698 5957 6086 9017 4698
Size: 1259 129 2931 6569
Fragments arranged by size:
6569 2931 1259 129
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4649 8696 276
Size: 53 83 186 936 3115 4047 2468
Fragments arranged by size:
4047 3115 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI
PstI PvuI PvuII SacI SalI SanDI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BplI BsiWI
EcoRV FseI FspAI NruI PacI PfoI PspXI RsrII
SacII SexAI SrfI SwaI