Restriction map for
pFN21AA1207
(Linear) (Six-base) MAPSORT of: KIAA1207.seq Check: 26 from: 1 to: 6183
KIAA1207.orf
With 182 enzymes: SgfI *
July 18, 2007 13:35 ..
AatII G_ACGT'C
Cuts at: 0 2646 6183
Size: 2646 3537
AccI GT'mk_AC
Cuts at: 0 168 3407 6183
Size: 168 3239 2776
Fragments arranged by size:
3239 2776 168
AclI AA'CG_TT
Cuts at: 0 1492 6183
Size: 1492 4691
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 1264 1280 2095 2315 2473 3230 5189
Size: 283 981 16 815 220 158 757 1959
Cuts at: 5189 5263 6183
Size: 74 920
Fragments arranged by size:
1959 981 920 815 757 283 220 158
74 16
AflII C'TTAA_G
Cuts at: 0 2523 3936 4036 6183
Size: 2523 1413 100 2147
Fragments arranged by size:
2523 2147 1413 100
AflIII A'CryG_T
Cuts at: 0 2111 6183
Size: 2111 4072
AleI CACnn'nnGTG
Cuts at: 0 671 2236 3358 6183
Size: 671 1565 1122 2825
Fragments arranged by size:
2825 1565 1122 671
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 310 6183
Size: 310 5873
AlwNI CAG_nnn'CTG
Cuts at: 0 3224 5208 6183
Size: 3224 1984 975
Fragments arranged by size:
3224 1984 975
ApaI G_GGCC'C
Cuts at: 0 413 490 497 1905 5166 6183
Size: 413 77 7 1408 3261 1017
Fragments arranged by size:
3261 1408 1017 413 77 7
ApaLI G'TGCA_C
Cuts at: 0 4173 6183
Size: 4173 2010
ApoI r'AATT_y
Cuts at: 0 46 835 2042 2369 2689 3178 3673 4366
Size: 46 789 1207 327 320 489 495 693
Cuts at: 4366 4468 4856 5125 5526 5626 6183
Size: 102 388 269 401 100 557
Fragments arranged by size:
1207 789 693 557 495 489 401 388
327 320 269 102 100 46
AseI AT'TA_AT
Cuts at: 0 238 2281 6183
Size: 238 2043 3902
Fragments arranged by size:
3902 2043 238
AvaI C'yCGr_G
Cuts at: 0 2824 4886 5158 6183
Size: 2824 2062 272 1025
Fragments arranged by size:
2824 2062 1025 272
BamHI G'GATC_C
Cuts at: 0 60 1185 6183
Size: 60 1125 4998
Fragments arranged by size:
4998 1125 60
BanI G'GyrC_C
Cuts at: 0 2487 3286 3749 3858 5872 6062 6183
Size: 2487 799 463 109 2014 190 121
Fragments arranged by size:
2487 2014 799 463 190 121 109
BanII G_rGCy'C
Cuts at: 0 413 490 497 1667 1905 4567 4694 5166
Size: 413 77 7 1170 238 2662 127 472
Cuts at: 5166 6183
Size: 1017
Fragments arranged by size:
2662 1170 1017 472 413 238 127 77
7
BbeI G_GCGC'C
Cuts at: 0 3862 5876 6066 6183
Size: 3862 2014 190 117
Fragments arranged by size:
3862 2014 190 117
BbsI GAAGACnn'nnnn_
Cuts at: 0 467 1645 2105 4048 4304 4769 6183
Size: 467 1178 460 1943 256 465 1414
Fragments arranged by size:
1943 1414 1178 467 465 460 256
BbvCI CC'TCA_GC
Cuts at: 0 1100 6183
Size: 1100 5083
BciVI GTATCCnnnnn_n'
Cuts at: 0 2024 2468 6183
Size: 2024 444 3715
Fragments arranged by size:
3715 2024 444
BclI T'GATC_A
Cuts at: 0 3003 4025 4121 5375 5648 6183
Size: 3003 1022 96 1254 273 535
Fragments arranged by size:
3003 1254 1022 535 273 96
BfrBI ATG'CAT
Cuts at: 0 2413 2935 6183
Size: 2413 522 3248
Fragments arranged by size:
3248 2413 522
BglI GCCn_nnn'nGGC
Cuts at: 0 501 3295 6183
Size: 501 2794 2888
Fragments arranged by size:
2888 2794 501
BglII A'GATC_T
Cuts at: 0 5506 6183
Size: 5506 677
BlpI GC'TnA_GC
Cuts at: 0 527 6183
Size: 527 5656
Bme1580I G_kGCm'C
Cuts at: 0 413 490 497 1905 3291 4177 5166 6183
Size: 413 77 7 1408 1386 886 989 1017
Fragments arranged by size:
1408 1386 1017 989 886 413 77 7
BmgBI CAC'GTC
Cuts at: 0 669 6183
Size: 669 5514
BmrI ACTGGGnnnn_n'
Cuts at: 0 350 1341 1445 1947 3748 3974 4753 4792
Size: 350 991 104 502 1801 226 779 39
Cuts at: 4792 6183
Size: 1391
Fragments arranged by size:
1801 1391 991 779 502 350 226 104
39
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2894 3246 3352 4678 5006 5628 6183
Size: 2894 352 106 1326 328 622 555
Fragments arranged by size:
2894 1326 622 555 352 328 106
Bpu10I CC'TnA_GC
Cuts at: 0 403 1100 5216 6183
Size: 403 697 4116 967
Fragments arranged by size:
4116 967 697 403
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 180 1654 1681 2665 4417 4823 6183
Size: 180 1474 27 984 1752 406 1360
Fragments arranged by size:
1752 1474 1360 984 406 180 27
BsaI GGTCTCn'nnnn_
Cuts at: 0 2189 5007 6183
Size: 2189 2818 1176
Fragments arranged by size:
2818 2189 1176
BsaAI yAC'GTr
Cuts at: 0 3554 5223 6183
Size: 3554 1669 960
Fragments arranged by size:
3554 1669 960
BsaHI Gr'CG_yC
Cuts at: 0 2643 3859 5873 6063 6183
Size: 2643 1216 2014 190 120
Fragments arranged by size:
2643 2014 1216 190 120
BsaWI w'CCGG_w
Cuts at: 0 973 1064 5772 6183
Size: 973 91 4708 411
Fragments arranged by size:
4708 973 411 91
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2527 2557 2892 2922 2982 3012 3995 4025
Size: 2527 30 335 30 60 30 983 30
Cuts at: 4025 4087 4117 6183
Size: 62 30 2066
Fragments arranged by size:
2527 2066 983 335 62 60 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 446 977 3243 4016 4984 6121 6183
Size: 446 531 2266 773 968 1137 62
Fragments arranged by size:
2266 1137 968 773 531 446 62
BseYI C'CCAG_C
Cuts at: 0 412 2711 2890 2975 3149 4597 5887 6183
Size: 412 2299 179 85 174 1448 1290 296
Fragments arranged by size:
2299 1448 1290 412 296 179 174 85
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3281 5131 6183
Size: 3281 1850 1052
Fragments arranged by size:
3281 1850 1052
BsiEI CG_ry'CG
Cuts at: 0 2846 6183
Size: 2846 3337
BsiHKAI G_wGCw'C
Cuts at: 0 4177 4694 5222 6131 6183
Size: 4177 517 528 909 52
Fragments arranged by size:
4177 909 528 517 52
BsmI GAATG_Cn'
Cuts at: 0 2415 4660 6183
Size: 2415 2245 1523
Fragments arranged by size:
2415 2245 1523
BsmBI CGTCTCn'nnnn_
Cuts at: 0 990 6183
Size: 990 5193
Bsp1286I G_dGCh'C
Cuts at: 0 413 490 497 1667 1905 3291 4177 4567
Size: 413 77 7 1170 238 1386 886 390
Cuts at: 4567 4694 5166 5222 6131 6183
Size: 127 472 56 909 52
Fragments arranged by size:
1386 1170 909 886 472 413 390 238
127 77 56 52 7
BspEI T'CCGG_A
Cuts at: 0 5772 6183
Size: 5772 411
BspHI T'CATG_A
Cuts at: 0 884 4073 6183
Size: 884 3189 2110
Fragments arranged by size:
3189 2110 884
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1691 2764 5375 5747 6183
Size: 1691 1073 2611 372 436
Fragments arranged by size:
2611 1691 1073 436 372
BsrBI CCG'CTC
Cuts at: 0 407 2959 5175 6183
Size: 407 2552 2216 1008
Fragments arranged by size:
2552 2216 1008 407
BsrDI GCAATG_nn'
Cuts at: 0 1055 1167 5475 6183
Size: 1055 112 4308 708
Fragments arranged by size:
4308 1055 708 112
BsrFI r'CCGG_y
Cuts at: 0 563 2841 6183
Size: 563 2278 3342
Fragments arranged by size:
3342 2278 563
BsrGI T'GTAC_A
Cuts at: 0 1225 3869 4103 6183
Size: 1225 2644 234 2080
Fragments arranged by size:
2644 2080 1225 234
BssSI C'ACGA_G
Cuts at: 0 1180 2103 6183
Size: 1180 923 4080
Fragments arranged by size:
4080 1180 923
BstBI TT'CG_AA
Cuts at: 0 5631 6183
Size: 5631 552
BstEII G'GTnAC_C
Cuts at: 0 277 1071 6183
Size: 277 794 5112
Fragments arranged by size:
5112 794 277
BstXI CCAn_nnnn'nTGG
Cuts at: 0 357 2711 5310 6183
Size: 357 2354 2599 873
Fragments arranged by size:
2599 2354 873 357
BstYI r'GATC_y
Cuts at: 0 60 289 599 1185 1607 2162 3480 4536
Size: 60 229 310 586 422 555 1318 1056
Cuts at: 4536 4574 5506 6183
Size: 38 932 677
Fragments arranged by size:
1318 1056 932 677 586 555 422 310
229 60 38
BstZ17I GTA'TAC
Cuts at: 0 169 6183
Size: 169 6014
Bsu36I CC'TnA_GG
Cuts at: 0 4380 5194 6183
Size: 4380 814 989
Fragments arranged by size:
4380 989 814
BtgI C'CryG_G
Cuts at: 0 3122 6183
Size: 3122 3061
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1939 6183
Size: 1939 4244
BtsI GCAGTG_nn'
Cuts at: 0 3908 4953 6183
Size: 3908 1045 1230
Fragments arranged by size:
3908 1230 1045
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1614 1649 6183
Size: 1614 35 4534
Fragments arranged by size:
4534 1614 35
DraI TTT'AAA
Cuts at: 0 3276 6183
Size: 3276 2907
DraIII CAC_nnn'GTG
Cuts at: 0 3259 6183
Size: 3259 2924
DrdI GACnn_nn'nnGTC
Cuts at: 0 478 5446 6183
Size: 478 4968 737
Fragments arranged by size:
4968 737 478
EaeI y'GGCC_r
Cuts at: 0 675 873 2843 4167 5848 6122 6183
Size: 675 198 1970 1324 1681 274 61
Fragments arranged by size:
1970 1681 1324 675 274 198 61
EagI C'GGCC_G
Cuts at: 0 2843 6183
Size: 2843 3340
EarI CTCTTCn'nnn_
Cuts at: 0 2291 2344 3319 4075 5165 5189 6121 6183
Size: 2291 53 975 756 1090 24 932 62
Fragments arranged by size:
2291 1090 975 932 756 62 53 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 365 582 1728 3202 4167 6183
Size: 365 217 1146 1474 965 2016
Fragments arranged by size:
2016 1474 1146 965 365 217
EcoICRI GAG'CTC
Cuts at: 0 4692 6183
Size: 4692 1491
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 1264 1280 2095 2315 2473 2894 3230
Size: 283 981 16 815 220 158 421 336
Cuts at: 3230 3246 3352 4678 5006 5189 5263 5628 6183
Size: 16 106 1326 328 183 74 365 555
Fragments arranged by size:
1326 981 815 555 421 365 336 328
283 220 183 158 106 74 16 16
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2705 4214 6183
Size: 2705 1509 1969
Fragments arranged by size:
2705 1969 1509
EcoO109I rG'GnC_Cy
Cuts at: 0 267 486 487 4476 4949 5162 5163 5323
Size: 267 219 1 3989 473 213 1 160
Cuts at: 5323 6183
Size: 860
Fragments arranged by size:
3989 860 473 267 219 213 160 1
1
EcoRI G'AATT_C
Cuts at: 0 2369 6183
Size: 2369 3814
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 547 3307 3489 4270 4584 5194 6006 6183
Size: 547 2760 182 781 314 610 812 177
Fragments arranged by size:
2760 812 781 610 547 314 182 177
HaeII r_GCGC'y
Cuts at: 0 3862 4188 5876 6066 6183
Size: 3862 326 1688 190 117
Fragments arranged by size:
3862 1688 326 190 117
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 169 201 987 1019 1114 1146 1408 1440
Size: 169 32 786 32 95 32 262 32
Cuts at: 1440 1592 1624 2227 2259 2398 2430 2495 2527
Size: 152 32 603 32 139 32 65 32
Cuts at: 2527 2724 2756 2892 2923 2924 2955 3395 3427
Size: 197 32 136 31 1 31 440 32
Cuts at: 3427 4566 4598 5815 5847 6183
Size: 1139 32 1217 32 336
Fragments arranged by size:
1217 1139 786 603 440 336 262 197
169 152 139 136 95 65 32 32
32 32 32 32 32 32 32 32
32 32 31 31 1
HincII GTy'rAC
Cuts at: 0 747 2900 6183
Size: 747 2153 3283
Fragments arranged by size:
3283 2153 747
HindIII A'AGCT_T
Cuts at: 0 1657 2751 6183
Size: 1657 1094 3432
Fragments arranged by size:
3432 1657 1094
KasI G'GCGC_C
Cuts at: 0 3858 5872 6062 6183
Size: 3858 2014 190 121
Fragments arranged by size:
3858 2014 190 121
MfeI C'AATT_G
Cuts at: 0 221 6183
Size: 221 5962
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 449 500 831 1341 1513 2204 3854 4502
Size: 449 51 331 510 172 691 1650 648
Cuts at: 4502 4530 5741 6183
Size: 28 1211 442
Fragments arranged by size:
1650 1211 691 648 510 449 442 331
172 51 28
MscI TGG'CCA
Cuts at: 0 677 875 5850 6183
Size: 677 198 4975 333
Fragments arranged by size:
4975 677 333 198
MslI CAynn'nnrTG
Cuts at: 0 395 671 2236 2332 3358 4078 4653 6183
Size: 395 276 1565 96 1026 720 575 1530
Fragments arranged by size:
1565 1530 1026 720 575 395 276 96
MspA1I CmG'CkG
Cuts at: 0 2058 2289 3265 5025 6183
Size: 2058 231 976 1760 1158
Fragments arranged by size:
2058 1760 1158 976 231
NaeI GCC'GGC
Cuts at: 0 565 2843 6183
Size: 565 2278 3340
Fragments arranged by size:
3340 2278 565
NarI GG'CG_CC
Cuts at: 0 3859 5873 6063 6183
Size: 3859 2014 190 120
Fragments arranged by size:
3859 2014 190 120
NcoI C'CATG_G
Cuts at: 0 3122 6183
Size: 3122 3061
NdeI CA'TA_TG
Cuts at: 0 3486 6183
Size: 3486 2697
NgoMIV G'CCGG_C
Cuts at: 0 563 2841 6183
Size: 563 2278 3342
Fragments arranged by size:
3342 2278 563
NsiI A_TGCA'T
Cuts at: 0 2415 2937 6183
Size: 2415 522 3246
Fragments arranged by size:
3246 2415 522
NspI r_CATG'y
Cuts at: 0 960 2316 4110 6183
Size: 960 1356 1794 2073
Fragments arranged by size:
2073 1794 1356 960
PasI CC'CwG_GG
Cuts at: 0 490 3882 5268 6183
Size: 490 3392 1386 915
Fragments arranged by size:
3392 1386 915 490
PflMI CCAn_nnn'nTGG
Cuts at: 0 1495 3020 4804 6183
Size: 1495 1525 1784 1379
Fragments arranged by size:
1784 1525 1495 1379
PmlI CAC'GTG
Cuts at: 0 3554 5223 6183
Size: 3554 1669 960
Fragments arranged by size:
3554 1669 960
PpuMI rG'GwC_Cy
Cuts at: 0 267 4949 6183
Size: 267 4682 1234
Fragments arranged by size:
4682 1234 267
PshAI GACnn'nnGTC
Cuts at: 0 5717 6183
Size: 5717 466
PsiI TTA'TAA
Cuts at: 0 797 6183
Size: 797 5386
PspOMI G'GGCC_C
Cuts at: 0 409 486 493 1901 5162 6183
Size: 409 77 7 1408 3261 1021
Fragments arranged by size:
3261 1408 1021 409 77 7
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1469 1501 6183
Size: 1469 32 4682
Fragments arranged by size:
4682 1469 32
PstI C_TGCA'G
Cuts at: 0 3801 6183
Size: 3801 2382
PvuII CAG'CTG
Cuts at: 0 2058 2289 3265 5025 6183
Size: 2058 231 976 1760 1158
Fragments arranged by size:
2058 1760 1158 976 231
SacI G_AGCT'C
Cuts at: 0 4694 6183
Size: 4694 1489
SanDI GG'GwC_CC
Cuts at: 0 4949 6183
Size: 4949 1234
SapI GCTCTTCn'nnn_
Cuts at: 0 2291 3319 5165 5189 6121 6183
Size: 2291 1028 1846 24 932 62
Fragments arranged by size:
2291 1846 1028 932 62 24
ScaI AGT'ACT
Cuts at: 0 4975 6183
Size: 4975 1208
SfcI C'TryA_G
Cuts at: 0 254 1596 3613 3797 5112 6183
Size: 254 1342 2017 184 1315 1071
Fragments arranged by size:
2017 1342 1315 1071 254 184
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 501 6183
Size: 501 5682
SfoI GGC'GCC
Cuts at: 0 3860 5874 6064 6183
Size: 3860 2014 190 119
Fragments arranged by size:
3860 2014 190 119
SmaI CCC'GGG
Cuts at: 0 2826 4888 6183
Size: 2826 2062 1295
Fragments arranged by size:
2826 2062 1295
SmlI C'TyrA_G
Cuts at: 0 159 1633 1660 2523 2680 3936 4036 4432
Size: 159 1474 27 863 157 1256 100 396
Cuts at: 4432 4838 6183
Size: 406 1345
Fragments arranged by size:
1474 1345 1256 863 406 396 159 157
100 27
SphI G_CATG'C
Cuts at: 0 2316 6183
Size: 2316 3867
SspI AAT'ATT
Cuts at: 0 1151 6183
Size: 1151 5032
StuI AGG'CCT
Cuts at: 0 651 3894 6183
Size: 651 3243 2289
Fragments arranged by size:
3243 2289 651
StyI C'CwwG_G
Cuts at: 0 192 497 1897 3122 3291 4091 5390 6183
Size: 192 305 1400 1225 169 800 1299 793
Fragments arranged by size:
1400 1299 1225 800 793 305 192 169
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1059 2904 3639 3750 3767 4062 4781 5277
Size: 1059 1845 735 111 17 295 719 496
Cuts at: 5277 5655 6183
Size: 378 528
Fragments arranged by size:
1845 1059 735 719 528 496 378 295
111 17
TatI w'GTAC_w
Cuts at: 0 1225 1535 1718 1853 2390 3869 4103 4973
Size: 1225 310 183 135 537 1479 234 870
Cuts at: 4973 6183
Size: 1210
Fragments arranged by size:
1479 1225 1210 870 537 310 234 183
135
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 578 610 2713 2745 2982 3014 3995 4027
Size: 578 32 2103 32 237 32 981 32
Cuts at: 4027 5051 5083 5715 5747 6183
Size: 1024 32 632 32 436
Fragments arranged by size:
2103 1024 981 632 578 436 237 32
32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 516 3403 4231 4642 6183
Size: 516 2887 828 411 1541
Fragments arranged by size:
2887 1541 828 516 411
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 354 2918 3450 3633 3987 4144 4185 4311
Size: 354 2564 532 183 354 157 41 126
Cuts at: 4311 4708 6183
Size: 397 1475
Fragments arranged by size:
2564 1475 532 397 354 354 183 157
126 41
XmaI C'CCGG_G
Cuts at: 0 2824 4886 6183
Size: 2824 2062 1297
Fragments arranged by size:
2824 2062 1297
XmnI GAAnn'nnTTC
Cuts at: 0 2693 3952 4081 6183
Size: 2693 1259 129 2102
Fragments arranged by size:
2693 2102 1259 129
ZraI GAC'GTC
Cuts at: 0 2644 6183
Size: 2644 3539
Enzymes that do cut:
AatII AccI AclI AcuI AflII AflIII AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII
KasI MfeI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NsiI NspI PasI PflMI PmlI
PpuMI PshAI PsiI PspOMI PsrI PstI PvuII SacI
SanDI SapI ScaI SfcI SfiI SfoI SmaI SmlI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AfeI AgeI AhdI AloI AscI AsiSI
AvrII BaeI BcgI BmtI BplI BsaBI BsiWI BssHII
BstAPI ClaI EcoRV FseI FspI FspAI HpaI KpnI
MluI NheI NotI NruI PacI PciI PfoI PmeI
PpiI PspXI PvuI RsrII SacII SalI SbfI SexAI
SgfI SgrAI SnaBI SpeI SrfI SwaI XbaI XhoI