(Circular) (Six-base) MAPSORT of: pFN21AA1113.seq Check: 6896 from: 1 to: 8086 pFN21AA1113 8086 bp With 182 enzymes: SgfI * February 27, 2009 14:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2384 3217 2384 Size: 833 7253 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5896 278 Size: 53 83 186 936 4360 2468 Fragments arranged by size: 4360 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5429 1937 Size: 3492 4594 Acc65I G'GTAC_C Cuts at: 5407 5407 Size: 8086 AclI AA'CG_TT Cuts at: 1563 6213 6586 1563 Size: 4650 373 3063 Fragments arranged by size: 4650 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2634 4368 5018 6149 7533 7801 1632 Size: 1002 1734 650 1131 1384 268 1917 Fragments arranged by size: 1917 1734 1384 1131 1002 650 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7889 AflIII A'CryG_T Cuts at: 1203 2723 2873 2888 6985 7962 1203 Size: 1520 150 15 4097 977 1327 Fragments arranged by size: 4097 1520 1327 977 150 15 AgeI A'CCGG_T Cuts at: 6917 6917 Size: 8086 AhdI GACnn_n'nnGTC Cuts at: 6815 6815 Size: 8086 AleI CACnn'nnGTG Cuts at: 1166 2123 4220 1166 Size: 957 2097 5032 Fragments arranged by size: 5032 2097 957 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8086 AlwNI CAG_nnn'CTG Cuts at: 2798 3455 3629 5829 7401 2798 Size: 657 174 2200 1572 3483 Fragments arranged by size: 3483 2200 1572 657 174 ApaI G_GGCC'C Cuts at: 2091 2190 2091 Size: 99 7987 ApaLI G'TGCA_C Cuts at: 6143 7299 6143 Size: 1156 6930 ApoI r'AATT_y Cuts at: 1493 2750 4946 5395 5621 5773 7882 1493 Size: 1257 2196 449 226 152 2109 1697 Fragments arranged by size: 2196 2109 1697 1257 449 226 152 AseI AT'TA_AT Cuts at: 160 1032 3123 5166 6640 160 Size: 872 2091 2043 1474 1606 Fragments arranged by size: 2091 2043 1606 1474 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8086 AvaI C'yCGr_G Cuts at: 1870 1943 2218 2468 2500 5411 1870 Size: 73 275 250 32 2911 4545 Fragments arranged by size: 4545 2911 275 250 73 32 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2912 2945 2912 Size: 33 8053 BamHI G'GATC_C Cuts at: 5416 5783 5416 Size: 367 7719 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2544 5407 6862 618 Size: 325 217 627 757 2863 1455 1842 Fragments arranged by size: 2863 1842 1455 757 627 325 217 BanII G_rGCy'C Cuts at: 1391 2091 2190 2225 2424 3938 4566 5405 1391 Size: 700 99 35 199 1514 628 839 4072 Fragments arranged by size: 4072 1514 839 700 628 199 99 35 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2432 2673 4298 4671 4901 5331 928 Size: 971 533 241 1625 373 230 430 3683 Fragments arranged by size: 3683 1625 971 533 430 373 241 230 BbvCI CC'TCA_GC Cuts at: 2169 2169 Size: 8086 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6277 6311 1903 Size: 34 4340 34 3678 Fragments arranged by size: 4340 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3801 5978 7188 3801 Size: 2177 1210 4699 Fragments arranged by size: 4699 2177 1210 BclI T'GATC_A Cuts at: 1548 2769 3711 4315 1548 Size: 1221 942 604 5319 Fragments arranged by size: 5319 1221 942 604 BfrBI ATG'CAT Cuts at: 4493 4493 Size: 8086 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6697 136 Size: 107 122 71 631 605 5025 1525 Fragments arranged by size: 5025 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4696 5241 8081 4696 Size: 545 2840 4701 Fragments arranged by size: 4701 2840 545 BlpI GC'TnA_GC Cuts at: 2486 4565 5503 2486 Size: 2079 938 5069 Fragments arranged by size: 5069 2079 938 Bme1580I G_kGCm'C Cuts at: 1790 2091 2190 2264 2496 2549 6147 7303 1790 Size: 301 99 74 232 53 3598 1156 2573 Fragments arranged by size: 3598 2573 1156 301 232 99 74 53 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8086 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2543 5817 6775 Size: 416 53 83 399 24 1126 3274 958 Cuts at: 6775 442 Size: 1753 Fragments arranged by size: 3274 1753 1126 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8086 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3798 4715 5263 3798 Size: 917 548 6621 Fragments arranged by size: 6621 917 548 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2283 2286 2289 3477 3536 4149 6746 1239 Size: 1044 3 3 1188 59 613 2597 2579 Fragments arranged by size: 2597 2579 1188 1044 613 59 3 3 Bpu10I CC'TnA_GC Cuts at: 2169 5193 2169 Size: 3024 5062 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3017 4066 6211 7076 7374 3017 Size: 1049 2145 865 298 3729 Fragments arranged by size: 3729 2145 1049 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4219 5362 6749 882 Size: 617 2720 1143 1387 2219 Fragments arranged by size: 2720 2219 1387 1143 617 BsaAI yAC'GTr Cuts at: 493 1204 5877 493 Size: 711 4673 2702 Fragments arranged by size: 4673 2702 711 BsaBI GATnn'nnATC Cuts at: 8080 8080 Size: 8086 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2291 5893 6275 6910 Size: 53 83 186 936 758 3602 382 635 Cuts at: 6910 275 Size: 1451 Fragments arranged by size: 3602 1451 936 758 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6519 6917 7191 7338 7749 6519 Size: 398 274 147 411 6856 Fragments arranged by size: 6856 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2281 2311 1178 Size: 30 165 30 878 30 6953 Fragments arranged by size: 6953 878 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2150 2153 2156 2159 2206 3693 4423 4644 Size: 964 3 3 3 47 1487 730 221 Cuts at: 4644 4707 1186 Size: 63 4565 Fragments arranged by size: 4565 1487 964 730 221 63 47 3 3 3 BseYI C'CCAG_C Cuts at: 7289 7785 7289 Size: 496 7590 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2700 3056 1163 Size: 573 964 356 6193 Fragments arranged by size: 6193 964 573 356 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2184 5536 6297 7325 1540 Size: 275 186 183 3352 761 1028 2301 Fragments arranged by size: 3352 2301 1028 761 275 186 183 BsiHKAI G_wGCw'C Cuts at: 6147 6232 7303 6147 Size: 85 1071 6930 Fragments arranged by size: 6930 1071 85 BsmI GAATG_Cn' Cuts at: 2570 3793 5597 5690 2570 Size: 1223 1804 93 4966 Fragments arranged by size: 4966 1804 1223 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2091 2190 2225 2264 2424 2496 2549 Size: 399 301 99 35 39 160 72 53 Cuts at: 2549 3938 4566 5405 6147 6232 7303 1391 Size: 1389 628 839 742 85 1071 2174 Fragments arranged by size: 2174 1389 1071 839 742 628 399 301 160 99 85 72 53 39 35 BspEI T'CCGG_A Cuts at: 7749 7749 Size: 8086 BspHI T'CATG_A Cuts at: 5975 7705 5975 Size: 1730 6356 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2384 3217 4476 4537 5441 844 Size: 1540 833 1259 61 904 3489 Fragments arranged by size: 3489 1540 1259 904 833 61 BsrBI CCG'CTC Cuts at: 1394 1823 2040 2321 5973 1394 Size: 429 217 281 3652 3507 Fragments arranged by size: 3652 3507 429 281 217 BsrDI GCAATG_nn' Cuts at: 65 1248 6581 6755 65 Size: 1183 5333 174 1396 Fragments arranged by size: 5333 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2065 2162 2293 2406 6730 6917 1700 Size: 110 255 97 131 113 4324 187 2869 Fragments arranged by size: 4324 2869 255 187 131 113 110 97 BsrGI T'GTAC_A Cuts at: 96 2836 96 Size: 2740 5346 BssHII G'CGCG_C Cuts at: 1925 2061 1925 Size: 136 7950 BssSI C'ACGA_G Cuts at: 6146 7158 6146 Size: 1012 7074 BstAPI GCAn_nnn'nTGC Cuts at: 794 2398 3727 794 Size: 1604 1329 5153 Fragments arranged by size: 5153 1604 1329 BstBI TT'CG_AA Cuts at: 4597 6890 4597 Size: 2293 5793 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8086 BstXI CCAn_nnnn'nTGG Cuts at: 2531 4066 2531 Size: 1535 6551 BstYI r'GATC_y Cuts at: 1972 4696 5241 5416 5783 6168 6185 7637 7723 Size: 2724 545 175 367 385 17 1452 86 Cuts at: 7723 7735 8081 1972 Size: 12 346 1977 Fragments arranged by size: 2724 1977 1452 545 385 367 346 175 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2121 2208 4884 513 Size: 552 939 117 87 2676 3715 Fragments arranged by size: 3715 2676 939 552 117 87 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4023 487 Size: 683 56 2797 4550 Fragments arranged by size: 4550 2797 683 56 BtsI GCAGTG_nn' Cuts at: 5602 6396 6416 5602 Size: 794 20 7272 Fragments arranged by size: 7272 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8086 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8051 DraI TTT'AAA Cuts at: 5390 5390 Size: 8086 DraIII CAC_nnn'GTG Cuts at: 3344 3344 Size: 8086 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7093 809 Size: 507 5777 1802 Fragments arranged by size: 5777 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2181 2205 3096 Size: 54 1425 50 275 18 351 24 891 Cuts at: 3096 5079 5533 6422 8051 8 Size: 1983 454 889 1629 43 Fragments arranged by size: 1983 1629 1425 891 889 454 351 275 54 50 43 24 18 EagI C'GGCC_G Cuts at: 1537 1812 2181 5533 1537 Size: 275 369 3352 4090 Fragments arranged by size: 4090 3352 369 275 EarI CTCTTCn'nnn_ Cuts at: 1353 5283 6016 1353 Size: 3930 733 3423 Fragments arranged by size: 3930 3423 733 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 2120 2432 6673 7047 7193 Size: 1064 138 435 96 312 4241 374 146 Cuts at: 7193 387 Size: 1280 Fragments arranged by size: 4241 1280 1064 435 374 312 146 138 96 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2283 2286 2289 2634 3477 3536 4149 Size: 393 651 3 3 345 843 59 613 Cuts at: 4149 4368 5018 6149 6746 7533 7801 1239 Size: 219 650 1131 597 787 268 1524 Fragments arranged by size: 1524 1131 843 787 651 650 613 597 393 345 268 219 59 3 3 EcoNI CCTnn'n_nnAGG Cuts at: 4164 4164 Size: 8086 EcoO109I rG'GnC_Cy Cuts at: 2327 3971 4169 5256 2327 Size: 1644 198 1087 5157 Fragments arranged by size: 5157 1644 1087 198 EcoRI G'AATT_C Cuts at: 4946 5395 5773 4946 Size: 449 378 7259 Fragments arranged by size: 7259 449 378 FspI TGC'GCA Cuts at: 4869 6592 4869 Size: 1723 6363 HaeII r_GCGC'y Cuts at: 1678 2169 2482 7233 1678 Size: 491 313 4751 2531 Fragments arranged by size: 4751 2531 491 313 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2281 2313 2624 2656 4064 Size: 32 135 32 709 32 311 32 1408 Cuts at: 4064 4096 4438 4470 6727 6759 6801 6833 1373 Size: 32 342 32 2257 32 42 32 2626 Fragments arranged by size: 2626 2257 1408 709 342 311 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2816 5430 5676 669 Size: 988 281 878 2614 246 3079 Fragments arranged by size: 3079 2614 988 878 281 246 HindIII A'AGCT_T Cuts at: 748 2946 5030 748 Size: 2198 2084 3804 Fragments arranged by size: 3804 2198 2084 HpaI GTT'AAC Cuts at: 5676 5676 Size: 8086 KpnI G_GTAC'C Cuts at: 5411 5411 Size: 8086 MfeI C'AATT_G Cuts at: 2970 3261 5685 2970 Size: 291 2424 5371 Fragments arranged by size: 5371 2424 291 MluI A'CGCG_T Cuts at: 7962 7962 Size: 8086 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2782 4047 4775 7200 7384 1305 Size: 1477 1265 728 2425 184 2007 Fragments arranged by size: 2425 2007 1477 1265 728 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3098 10 Size: 54 1425 343 1266 4998 Fragments arranged by size: 4998 1425 1266 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2123 4220 6044 6403 6562 518 Size: 648 957 2097 1824 359 159 2042 Fragments arranged by size: 2097 2042 1824 957 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2055 2123 2210 3822 4263 5502 6179 7327 Size: 449 68 87 1612 441 1239 677 1148 Cuts at: 7327 7572 1606 Size: 245 2120 Fragments arranged by size: 2120 1612 1239 1148 677 449 441 245 87 68 NaeI GCC'GGC Cuts at: 2164 2295 2408 2164 Size: 131 113 7842 Fragments arranged by size: 7842 131 113 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6595 Fragments arranged by size: 6595 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8086 NgoMIV G'CCGG_C Cuts at: 2162 2293 2406 2162 Size: 131 113 7842 Fragments arranged by size: 7842 131 113 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8086 NotI GC'GGCC_GC Cuts at: 5533 5533 Size: 8086 NsiI A_TGCA'T Cuts at: 4495 4495 Size: 8086 NspI r_CATG'y Cuts at: 1132 1229 2727 2877 2892 3239 3562 3884 4423 Size: 97 1498 150 15 347 323 322 539 Cuts at: 4423 5444 6989 1132 Size: 1021 1545 2229 Fragments arranged by size: 2229 1545 1498 1021 539 347 323 322 150 97 15 PasI CC'CwG_GG Cuts at: 1348 3875 1348 Size: 2527 5559 PciI A'CATG_T Cuts at: 2723 2873 2888 6985 2723 Size: 150 15 4097 3824 Fragments arranged by size: 4097 3824 150 15 PflMI CCAn_nnn'nTGG Cuts at: 1108 3399 3455 1108 Size: 2291 56 5739 Fragments arranged by size: 5739 2291 56 PfoI T'CCnGG_A Cuts at: 2347 2367 4186 4950 5064 2347 Size: 20 1819 764 114 5369 Fragments arranged by size: 5369 1819 764 114 20 PmeI GTTT'AAAC Cuts at: 5390 5390 Size: 8086 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6155 6187 7668 7700 6155 Size: 32 1481 32 6541 Fragments arranged by size: 6541 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2327 3971 4169 5256 2327 Size: 1644 198 1087 5157 Fragments arranged by size: 5157 1644 1087 198 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8086 PsiI TTA'TAA Cuts at: 4994 5656 4994 Size: 662 7424 PspOMI G'GGCC_C Cuts at: 2087 2186 2087 Size: 99 7987 PstI C_TGCA'G Cuts at: 830 4534 5438 830 Size: 3704 904 3478 Fragments arranged by size: 3704 3478 904 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8086 PvuII CAG'CTG Cuts at: 4263 4263 Size: 8086 SacII CC_GC'GG Cuts at: 2124 2211 2124 Size: 87 7999 SalI G'TCGA_C Cuts at: 1936 5428 1936 Size: 3492 4594 SanDI GG'GwC_CC Cuts at: 2327 2327 Size: 8086 SbfI CC_TGCA'GG Cuts at: 5438 5438 Size: 8086 ScaI AGT'ACT Cuts at: 4237 4246 6334 8046 4237 Size: 9 2088 1712 4277 Fragments arranged by size: 4277 2088 1712 9 SfcI C'TryA_G Cuts at: 826 1045 3191 3942 4530 5434 6569 7250 7441 Size: 219 2146 751 588 904 1135 681 191 Cuts at: 7441 826 Size: 1471 Fragments arranged by size: 2146 1471 1135 904 751 681 588 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8086 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8086 SmaI CCC'GGG Cuts at: 1872 2502 5413 1872 Size: 630 2911 4545 Fragments arranged by size: 4545 2911 630 SmlI C'TyrA_G Cuts at: 820 1017 1943 2996 4081 6190 7091 7353 820 Size: 197 926 1053 1085 2109 901 262 1553 Fragments arranged by size: 2109 1553 1085 1053 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5877 493 Size: 5384 2702 SpeI A'CTAG_T Cuts at: 152 2657 152 Size: 2505 5581 SphI G_CATG'C Cuts at: 1132 3884 5444 1132 Size: 2752 1560 3774 Fragments arranged by size: 3774 2752 1560 SspI AAT'ATT Cuts at: 5 52 3833 6010 5 Size: 47 3781 2177 2081 Fragments arranged by size: 3781 2177 2081 47 StuI AGG'CCT Cuts at: 1517 3216 1517 Size: 1699 6387 StyI C'CwwG_G Cuts at: 513 1065 2004 5525 513 Size: 552 939 3521 3074 Fragments arranged by size: 3521 3074 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4072 6113 6130 6283 6468 6913 325 Size: 1255 2492 2041 17 153 185 445 1498 Fragments arranged by size: 2492 2041 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2654 2836 4235 Size: 274 80 33 51 1443 677 182 1399 Cuts at: 4235 4244 5217 5374 6332 8044 96 Size: 9 973 157 958 1712 138 Fragments arranged by size: 1712 1443 1399 973 958 677 274 182 157 138 80 51 33 9 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4141 4173 8046 8078 4141 Size: 32 3873 32 4149 Fragments arranged by size: 4149 3873 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8086 XbaI T'CTAG_A Cuts at: 4474 5422 4474 Size: 948 7138 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4665 4776 4665 Size: 111 7975 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8086 XmaI C'CCGG_G Cuts at: 1870 2500 5411 1870 Size: 630 2911 4545 Fragments arranged by size: 4545 2911 630 XmnI GAAnn'nnTTC Cuts at: 2861 2949 4032 4908 6215 2861 Size: 88 1083 876 1307 4732 Fragments arranged by size: 4732 1307 1083 876 88 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5894 276 Size: 53 83 186 936 4360 2468 Fragments arranged by size: 4360 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI PacI PmlI PspXI PsrI RsrII SacI SapI SexAI SfiI SfoI SrfI SwaI