(Circular) (Six-base) MAPSORT of: pFN21AA0800.seq Check: 1419 from: 1 to: 9226 pFN21AA0800 9226 bp With 182 enzymes: SgfI * May 17, 2016 16:10 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 5087 5303 5348 5087 Size: 216 45 8965 Fragments arranged by size: 8965 216 45 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7036 278 Size: 53 83 186 936 5500 2468 Fragments arranged by size: 5500 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6569 1937 Size: 4632 4594 Acc65I G'GTAC_C Cuts at: 2098 6547 2098 Size: 4449 4777 AclI AA'CG_TT Cuts at: 1563 7353 7726 1563 Size: 5790 373 3063 Fragments arranged by size: 5790 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2163 3110 3351 3617 3789 4034 5375 7289 Size: 531 947 241 266 172 245 1341 1914 Cuts at: 7289 8673 8941 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1914 1384 1341 947 531 268 266 245 241 172 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9029 AflIII A'CryG_T Cuts at: 1203 2023 3939 8125 9102 1203 Size: 820 1916 4186 977 1327 Fragments arranged by size: 4186 1916 1327 977 820 AgeI A'CCGG_T Cuts at: 3236 8057 3236 Size: 4821 4405 AhdI GACnn_n'nnGTC Cuts at: 7955 7955 Size: 9226 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 9226 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3590 4229 1184 Size: 2406 639 6181 Fragments arranged by size: 6181 2406 639 AlwNI CAG_nnn'CTG Cuts at: 4353 4369 4676 6184 6301 6969 8541 4353 Size: 16 307 1508 117 668 1572 5038 Fragments arranged by size: 5038 1572 1508 668 307 117 16 ApaI G_GGCC'C Cuts at: 2413 4273 2413 Size: 1860 7366 ApaLI G'TGCA_C Cuts at: 3510 7283 8439 3510 Size: 3773 1156 4297 Fragments arranged by size: 4297 3773 1156 ApoI r'AATT_y Cuts at: 1493 2506 3161 3173 3493 3722 4032 4106 6535 Size: 1013 655 12 320 229 310 74 2429 Cuts at: 6535 6761 6913 9022 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2429 2109 1697 1013 655 320 310 229 226 152 74 12 AseI AT'TA_AT Cuts at: 160 1032 5829 7780 160 Size: 872 4797 1951 1606 Fragments arranged by size: 4797 1951 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9226 AvaI C'yCGr_G Cuts at: 1870 1943 2412 6551 1870 Size: 73 469 4139 4545 Fragments arranged by size: 4545 4139 469 73 BamHI G'GATC_C Cuts at: 2161 6556 6923 2161 Size: 4395 367 4464 Fragments arranged by size: 4464 4395 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2098 2942 3901 4763 4949 Size: 325 217 627 311 844 959 862 186 Cuts at: 4949 5223 5336 6547 8002 618 Size: 274 113 1211 1455 1842 Fragments arranged by size: 1842 1455 1211 959 862 844 627 325 311 274 217 186 113 BanII G_rGCy'C Cuts at: 1391 2046 2301 2413 3432 4273 6545 1391 Size: 655 255 112 1019 841 2272 4072 Fragments arranged by size: 4072 2272 1019 841 655 255 112 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2542 3443 3842 6432 928 Size: 971 643 901 399 2590 3722 Fragments arranged by size: 3722 2590 971 901 643 399 BbvCI CC'TCA_GC Cuts at: 4002 4797 4002 Size: 795 8431 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7417 7451 1903 Size: 34 5480 34 3678 Fragments arranged by size: 5480 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7118 8328 7118 Size: 1210 8016 BclI T'GATC_A Cuts at: 1548 3471 5823 1548 Size: 1923 2352 4951 Fragments arranged by size: 4951 2352 1923 BfrBI ATG'CAT Cuts at: 2276 3301 2276 Size: 1025 8201 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2417 7837 136 Size: 107 122 71 631 605 745 5420 1525 Fragments arranged by size: 5420 1525 745 631 605 122 107 71 BglII A'GATC_T Cuts at: 2718 5656 9221 2718 Size: 2938 3565 2723 Fragments arranged by size: 3565 2938 2723 BlpI GC'TnA_GC Cuts at: 2040 6643 2040 Size: 4603 4623 Bme1580I G_kGCm'C Cuts at: 1790 2413 3514 4273 7287 8443 1790 Size: 623 1101 759 3014 1156 2573 Fragments arranged by size: 3014 2573 1156 1101 759 623 BmgBI CAC'GTC Cuts at: 1318 3940 1318 Size: 2622 6604 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2586 3561 4516 Size: 416 53 83 399 24 1169 975 955 Cuts at: 4516 4664 6957 7915 442 Size: 148 2293 958 1753 Fragments arranged by size: 2293 1753 1169 975 958 955 416 399 148 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3543 4826 6389 1056 Size: 2487 1283 1563 3893 Fragments arranged by size: 3893 2487 1563 1283 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2078 2727 3902 5837 6323 7886 1239 Size: 839 649 1175 1935 486 1563 2579 Fragments arranged by size: 2579 1935 1563 1175 839 649 486 Bpu10I CC'TnA_GC Cuts at: 4002 4797 5118 4002 Size: 795 321 8110 Fragments arranged by size: 8110 795 321 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 7351 8216 8514 7351 Size: 865 298 8063 Fragments arranged by size: 8063 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2682 7889 882 Size: 617 1183 5207 2219 Fragments arranged by size: 5207 2219 1183 617 BsaAI yAC'GTr Cuts at: 493 1204 7017 493 Size: 711 5813 2702 Fragments arranged by size: 5813 2702 711 BsaBI GATnn'nnATC Cuts at: 2184 2880 4058 4244 9220 2184 Size: 696 1178 186 4976 2190 Fragments arranged by size: 4976 2190 1178 696 186 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5727 7033 7415 8050 Size: 53 83 186 936 4194 1306 382 635 Cuts at: 8050 275 Size: 1451 Fragments arranged by size: 4194 1451 1306 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3236 7659 8057 8331 8478 8889 3236 Size: 4423 398 274 147 411 3573 Fragments arranged by size: 4423 3573 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2979 3009 6296 6326 1178 Size: 30 165 30 1576 30 3287 30 4078 Fragments arranged by size: 4078 3287 1576 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2651 4113 5094 5399 6221 6332 6395 6416 Size: 1465 1462 981 305 822 111 63 21 Cuts at: 6416 6458 1186 Size: 42 3954 Fragments arranged by size: 3954 1465 1462 981 822 305 111 63 42 21 BseYI C'CCAG_C Cuts at: 2215 3742 4966 8429 8925 2215 Size: 1527 1224 3463 496 2516 Fragments arranged by size: 3463 2516 1527 1224 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 5326 5472 5733 1163 Size: 573 3590 146 261 4656 Fragments arranged by size: 4656 3590 573 261 146 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6676 7437 8465 1540 Size: 275 186 4675 761 1028 2301 Fragments arranged by size: 4675 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2046 3432 3514 4164 4218 7287 7372 8443 2046 Size: 1386 82 650 54 3069 85 1071 2829 Fragments arranged by size: 3069 2829 1386 1071 650 85 82 54 BsmI GAATG_Cn' Cuts at: 2266 2278 3651 5303 5524 6737 6830 2266 Size: 12 1373 1652 221 1213 93 4662 Fragments arranged by size: 4662 1652 1373 1213 221 93 12 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2046 2301 2413 3432 3514 4164 4218 Size: 399 256 255 112 1019 82 650 54 Cuts at: 4218 4273 6545 7287 7372 8443 1391 Size: 55 2272 742 85 1071 2174 Fragments arranged by size: 2272 2174 1071 1019 742 650 399 256 255 112 85 82 55 54 BspEI T'CCGG_A Cuts at: 8889 8889 Size: 9226 BspHI T'CATG_A Cuts at: 2256 3697 7115 8845 2256 Size: 1441 3418 1730 2637 Fragments arranged by size: 3418 2637 1730 1441 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3572 4638 5087 5303 5348 6581 844 Size: 2728 1066 449 216 45 1233 3489 Fragments arranged by size: 3489 2728 1233 1066 449 216 45 BsrBI CCG'CTC Cuts at: 1394 1823 5320 5745 5882 7113 1394 Size: 429 3497 425 137 1231 3507 Fragments arranged by size: 3507 3497 1231 429 425 137 BsrDI GCAATG_nn' Cuts at: 65 1248 2481 4728 4912 5598 7721 7895 65 Size: 1183 1233 2247 184 686 2123 174 1396 Fragments arranged by size: 2247 2123 1396 1233 1183 686 184 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3236 7870 8057 1700 Size: 110 1426 4634 187 2869 Fragments arranged by size: 4634 2869 1426 187 110 BsrGI T'GTAC_A Cuts at: 96 6077 96 Size: 5981 3245 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9226 BssSI C'ACGA_G Cuts at: 7286 8298 7286 Size: 1012 8214 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 9226 BstBI TT'CG_AA Cuts at: 2533 5851 8030 2533 Size: 3318 2179 3729 Fragments arranged by size: 3729 3318 2179 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9226 BstXI CCAn_nnnn'nTGG Cuts at: 3610 5810 3610 Size: 2200 7026 BstYI r'GATC_y Cuts at: 1972 2161 2718 3956 4359 4530 5656 6556 6923 Size: 189 557 1238 403 171 1126 900 367 Cuts at: 6923 7308 7325 8777 8863 8875 9221 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1238 1126 900 557 403 385 367 346 189 171 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2781 2781 Size: 9226 BtgI C'CryG_G Cuts at: 513 1065 2597 3282 4802 4945 513 Size: 552 1532 685 1520 143 4794 Fragments arranged by size: 4794 1532 1520 685 552 143 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8487 Fragments arranged by size: 8487 683 56 BtsI GCAGTG_nn' Cuts at: 2069 2089 2310 4162 4408 4674 6742 7536 7556 Size: 20 221 1852 246 266 2068 794 20 Cuts at: 7556 2069 Size: 3739 Fragments arranged by size: 3739 2068 1852 794 266 246 221 20 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9226 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3157 3192 529 Size: 35 2593 35 6563 Fragments arranged by size: 6563 2593 35 35 DraI TTT'AAA Cuts at: 2307 6530 2307 Size: 4223 5003 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2190 8233 809 Size: 507 874 6043 1802 Fragments arranged by size: 6043 1802 874 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3600 4170 6673 Size: 54 1425 50 275 18 1770 570 2503 Cuts at: 6673 7562 9191 8 Size: 889 1629 43 Fragments arranged by size: 2503 1770 1629 1425 889 570 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6673 1537 Size: 275 4861 4090 Fragments arranged by size: 4861 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2139 2243 2614 5967 6198 6213 7156 1353 Size: 786 104 371 3353 231 15 943 3423 Fragments arranged by size: 3423 3353 943 786 371 231 104 15 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5223 6323 7813 8187 8333 387 Size: 1064 138 3634 1100 1490 374 146 1280 Fragments arranged by size: 3634 1490 1280 1100 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2044 3430 2044 Size: 1386 7840 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2078 2163 2727 3110 3351 3617 3789 Size: 393 446 85 564 383 241 266 172 Cuts at: 3789 3902 4034 5375 5837 6323 7289 7886 8673 Size: 113 132 1341 462 486 966 597 787 Cuts at: 8673 8941 1239 Size: 268 1524 Fragments arranged by size: 1524 1341 966 787 597 564 486 462 446 393 383 268 266 241 172 132 113 85 EcoNI CCTnn'n_nnAGG Cuts at: 6398 6398 Size: 9226 EcoO109I rG'GnC_Cy Cuts at: 4270 5445 5845 4270 Size: 1175 400 7651 Fragments arranged by size: 7651 1175 400 EcoRI G'AATT_C Cuts at: 6535 6913 6535 Size: 378 8848 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4571 5545 4571 Size: 974 8252 FspI TGC'GCA Cuts at: 3183 3578 7732 3183 Size: 395 4154 4677 Fragments arranged by size: 4677 4154 395 HaeII r_GCGC'y Cuts at: 1678 5144 8373 1678 Size: 3466 3229 2531 Fragments arranged by size: 3466 3229 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2979 3011 3941 3948 3973 Size: 32 135 32 1407 32 930 7 25 Cuts at: 3973 3980 4880 4912 5729 5761 6413 6445 7867 Size: 7 900 32 817 32 652 32 1422 Cuts at: 7867 7899 7941 7973 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1422 1407 930 900 817 652 135 42 32 32 32 32 32 32 32 32 25 7 7 HincII GTy'rAC Cuts at: 669 1657 1938 5640 6570 6816 669 Size: 988 281 3702 930 246 3079 Fragments arranged by size: 3702 3079 988 930 281 246 HindIII A'AGCT_T Cuts at: 748 2700 748 Size: 1952 7274 HpaI GTT'AAC Cuts at: 6816 6816 Size: 9226 KpnI G_GTAC'C Cuts at: 2102 6551 2102 Size: 4449 4777 MfeI C'AATT_G Cuts at: 2123 3829 4063 6825 2123 Size: 1706 234 2762 4524 Fragments arranged by size: 4524 2762 1706 234 MluI A'CGCG_T Cuts at: 9102 9102 Size: 9226 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3924 4221 4920 6294 8340 8524 1305 Size: 2619 297 699 1374 2046 184 2007 Fragments arranged by size: 2619 2046 2007 1374 699 297 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3602 4172 10 Size: 54 1425 343 1770 570 5064 Fragments arranged by size: 5064 1770 1425 570 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2082 3044 4211 4238 5610 5808 6050 Size: 648 916 962 1167 27 1372 198 242 Cuts at: 6050 7184 7543 7702 518 Size: 1134 359 159 2042 Fragments arranged by size: 2042 1372 1167 1134 962 916 648 359 242 198 159 27 MspA1I CmG'CkG Cuts at: 1606 2515 3767 4353 4369 4984 5353 6642 7319 Size: 909 1252 586 16 615 369 1289 677 Cuts at: 7319 8467 8712 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1289 1252 1148 909 677 615 586 369 245 16 NcoI C'CATG_G Cuts at: 513 1065 3282 4945 513 Size: 552 2217 1663 4794 Fragments arranged by size: 4794 2217 1663 552 NdeI CA'TA_TG Cuts at: 387 2278 387 Size: 1891 7335 NheI G'CTAG_C Cuts at: 1052 3539 4822 6385 1052 Size: 2487 1283 1563 3893 Fragments arranged by size: 3893 2487 1563 1283 NotI GC'GGCC_GC Cuts at: 6673 6673 Size: 9226 NsiI A_TGCA'T Cuts at: 2278 3303 2278 Size: 1025 8201 NspI r_CATG'y Cuts at: 1132 1229 2027 2890 3301 3687 5054 6584 8129 Size: 97 798 863 411 386 1367 1530 1545 Cuts at: 8129 1132 Size: 2229 Fragments arranged by size: 2229 1545 1530 1367 863 798 411 386 97 PasI CC'CwG_GG Cuts at: 1348 2931 4813 1348 Size: 1583 1882 5761 Fragments arranged by size: 5761 1882 1583 PciI A'CATG_T Cuts at: 2023 8125 2023 Size: 6102 3124 PflMI CCAn_nnn'nTGG Cuts at: 1108 4179 5192 6301 1108 Size: 3071 1013 1109 4033 Fragments arranged by size: 4033 3071 1109 1013 PfoI T'CCnGG_A Cuts at: 5262 5262 Size: 9226 PmeI GTTT'AAAC Cuts at: 6530 6530 Size: 9226 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3995 4027 7295 7327 8808 8840 3995 Size: 32 3268 32 1481 32 4381 Fragments arranged by size: 4381 3268 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 5445 5845 5445 Size: 400 8826 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9226 PsiI TTA'TAA Cuts at: 6796 6796 Size: 9226 PspOMI G'GGCC_C Cuts at: 2409 4269 2409 Size: 1860 7366 PstI C_TGCA'G Cuts at: 830 2317 4392 4503 6578 830 Size: 1487 2075 111 2075 3478 Fragments arranged by size: 3478 2075 2075 1487 111 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9226 PvuII CAG'CTG Cuts at: 2515 3767 4353 4369 4984 5353 2515 Size: 1252 586 16 615 369 6388 Fragments arranged by size: 6388 1252 615 586 369 16 SacI G_AGCT'C Cuts at: 2046 3432 2046 Size: 1386 7840 SalI G'TCGA_C Cuts at: 1936 6568 1936 Size: 4632 4594 SbfI CC_TGCA'GG Cuts at: 6578 6578 Size: 9226 ScaI AGT'ACT Cuts at: 3478 7474 9186 3478 Size: 3996 1712 3518 Fragments arranged by size: 3996 3518 1712 SfcI C'TryA_G Cuts at: 826 1045 2010 2313 2494 2549 2555 2670 3960 Size: 219 965 303 181 55 6 115 1290 Cuts at: 3960 3972 4388 4499 4954 5700 6036 6574 7709 Size: 12 416 111 455 746 336 538 1135 Cuts at: 7709 8390 8581 826 Size: 681 191 1471 Fragments arranged by size: 1471 1290 1135 965 746 681 538 455 416 336 303 219 191 181 115 111 55 12 6 SfiI GGCCn_nnn'nGGCC Cuts at: 2417 2417 Size: 9226 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9226 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9226 SmaI CCC'GGG Cuts at: 1872 6553 1872 Size: 4681 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 7330 8231 8493 820 Size: 197 926 5387 901 262 1553 Fragments arranged by size: 5387 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 7017 493 Size: 6524 2702 SpeI A'CTAG_T Cuts at: 152 2750 152 Size: 2598 6628 SphI G_CATG'C Cuts at: 1132 3301 3687 6584 1132 Size: 2169 386 2897 3774 Fragments arranged by size: 3774 2897 2169 386 SspI AAT'ATT Cuts at: 5 52 3002 3534 7150 5 Size: 47 2950 532 3616 2081 Fragments arranged by size: 3616 2950 2081 532 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 9226 StyI C'CwwG_G Cuts at: 513 1065 3282 4945 6665 513 Size: 552 2217 1663 1720 3074 Fragments arranged by size: 3074 2217 1720 1663 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2923 3678 4751 7253 7270 7423 7608 Size: 1255 1343 755 1073 2502 17 153 185 Cuts at: 7608 8053 325 Size: 445 1498 Fragments arranged by size: 2502 1498 1343 1255 1073 755 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3193 3476 6077 Size: 274 80 33 51 1443 1216 283 2601 Cuts at: 6077 7472 9184 96 Size: 1395 1712 138 Fragments arranged by size: 2601 1712 1443 1395 1216 283 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3631 3663 5680 5712 5742 5774 5805 5837 9186 Size: 32 2017 32 30 32 31 32 3349 Cuts at: 9186 9218 3631 Size: 32 3639 Fragments arranged by size: 3639 3349 2017 32 32 32 32 32 31 30 Tth111I GACn'n_nGTC Cuts at: 1316 4409 1316 Size: 3093 6133 XbaI T'CTAG_A Cuts at: 6397 6562 6397 Size: 165 9061 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2854 2854 Size: 9226 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9226 XmaI C'CCGG_G Cuts at: 1870 6551 1870 Size: 4681 4545 XmnI GAAnn'nnTTC Cuts at: 2399 7355 2399 Size: 4956 4270 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7034 276 Size: 53 83 186 936 5500 2468 Fragments arranged by size: 5500 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SfoI SrfI SwaI