################################## # NO CUT ENZYMES ( 73 enzymes) # ################################## AatII AccIII AclI AflII AflIII AgeI AocI AscI AseI AsnI Asp700I AvrII BciVI BclI BfrI BlnI BsaBI BseAI BsiWI BsmBI BspDI BspEI BspHI BspLU11I BsrDI BssHII Bst1107I BstAPI BstBI BstZ17I Bsu36I ClaI CpoI DraI DrdI Eco81I EcoRV EcoT22I FbaI HincII HindII HpaI KspI MamI MfeI MluI MroI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I RcaI RsrII SacII SalI SfuI SgrAI SpeI SspI SwaI XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 26 enzymes) # ################################### Acc65I 265 GGTACC G^GTACC AccI 523 GTCTAC GT^[AC][GT]AC AhdI 382 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp718I 265 GGTACC G^GTACC AspEI 382 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 3536 TGCGCA TGC^GCA BbvCI 3254 CCTCAGC CCTCAGC(-5/-2) BcgI 3874 CGACAGCGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 2186 AGATCT A^GATCT BsmI 3061 GCATTC GAATGC(1/-1) Eam1105I 382 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 2082 GAAGAG CTCTTC(1/4) EcoRI 1279 GAATTC G^AATTC FspI 3536 TGCGCA TGC^GCA HindIII 4180 AAGCTT A^AGCTT KpnI 265 GGTACC GGTAC^C Ksp632I 2082 GAAGAG CTCTTC(1/4) MseI 752 TTAA T^TAA PvuI 3282 CGATCG CGAT^CG SacI 3385 GAGCTC GAGCT^C SapI 2082 GAAGAGC GCTCTTC(1/4) ScaI 1733 AGTACT AGT^ACT SexAI 1247 ACCTGGT A^CC[AT]GGT SnaBI 3664 TACGTA TAC^GTA SphI 4085 GCATGC GCATG^C Tru9I 752 TTAA T^TAA ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcsI 1279 GAATTC [GA]^AATT[CT] 1353 GAATTT ApoI 1279 GAATTC [GA]^AATT[CT] 1353 GAATTT AspI 1246 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1438 GACTGTGTC BamHI 2253 GGATCC G^GATCC 2927 GGATCC BbrPI 2532 CACGTG CAC^GTG 2559 CACGTG BbsI 1609 GAAGAC GAAGAC(2/6) 127 GTCTTC BfaI 2605 CTAG C^TAG 3197 CTAG BpuAI 1609 GAAGAC GAAGAC(2/6) 127 GTCTTC Bsp1407I 1805 TGTACA T^GTACA 2783 TGTACA BsrGI 1805 TGTACA T^GTACA 2783 TGTACA BstXI 1114 CCAGACAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3261 CCACTTCACTGG EcoNI 3119 CCTCGCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3513 CCTCACGCAGG FseI 10 GGCCGGCC GGCCGG^CC 14 GGCCGGCC MaeI 2605 CTAG C^TAG 3197 CTAG PflFI 1246 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1438 GACTGTGTC PmaCI 2532 CACGTG CAC^GTG 2559 CACGTG PmlI 2532 CACGTG CAC^GTG 2559 CACGTG PpuMI 494 AGGACCT [GA]G^G[AT]CC[CT] 1754 AGGACCT SbfI 1185 CCTGCAGG CCTGCA^GG 1749 CCTGCAGG Sse8387I 1185 CCTGCAGG CCTGCA^GG 1749 CCTGCAGG SspBI 1805 TGTACA T^GTACA 2783 TGTACA Tth111I 1246 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1438 GACTGTGTC ######################################### # 3 OR MORE CUT ENZYMES ( 137 enzymes) # ######################################### AccII AciI AcyI AfaI AluI Alw44I AlwI AlwNI Aor51HI ApaI ApaLI AspHI AvaI AvaII BalI BanI BanII BbeI BbvI BcnI BglI BlpI BmyI BpmI Bpu1102I BsaAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BssSI BstEII BstF5I BstNI BstPI BstUI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EagI EclXI Eco47III Eco52I EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfiI SfoI SmaI SmlI StuI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI