(Circular) (Six-base) MAPSORT of: pFN21AA0671.seq Check: 8329 from: 1 to: 6313 pFN21AA0671 6313 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3474 3474 Size: 6313 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4123 278 Size: 53 83 186 936 2587 2468 Fragments arranged by size: 2587 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3656 1937 Size: 1719 4594 Acc65I G'GTAC_C Cuts at: 3634 3634 Size: 6313 AclI AA'CG_TT Cuts at: 1563 4440 4813 1563 Size: 2877 373 3063 Fragments arranged by size: 3063 2877 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3222 4376 5760 6028 1632 Size: 1590 1154 1384 268 1917 Fragments arranged by size: 1917 1590 1384 1154 268 AflII C'TTAA_G Cuts at: 820 1017 2179 820 Size: 197 1162 4954 Fragments arranged by size: 4954 1162 197 AflIII A'CryG_T Cuts at: 1203 5212 6189 1203 Size: 4009 977 1327 Fragments arranged by size: 4009 1327 977 AgeI A'CCGG_T Cuts at: 5144 5144 Size: 6313 AhdI GACnn_n'nnGTC Cuts at: 5042 5042 Size: 6313 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6313 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3230 1184 Size: 2046 4267 AlwNI CAG_nnn'CTG Cuts at: 2720 4056 5628 2720 Size: 1336 1572 3405 Fragments arranged by size: 3405 1572 1336 ApaLI G'TGCA_C Cuts at: 3487 4370 5526 3487 Size: 883 1156 4274 Fragments arranged by size: 4274 1156 883 ApoI r'AATT_y Cuts at: 1493 2224 3622 3848 4000 6109 1493 Size: 731 1398 226 152 2109 1697 Fragments arranged by size: 2109 1697 1398 731 226 152 AseI AT'TA_AT Cuts at: 160 1032 2642 4867 160 Size: 872 1610 2225 1606 Fragments arranged by size: 2225 1610 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6313 AvaI C'yCGr_G Cuts at: 1870 1943 3638 1870 Size: 73 1695 4545 Fragments arranged by size: 4545 1695 73 BamHI G'GATC_C Cuts at: 3643 4010 3643 Size: 367 5946 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3634 5089 618 Size: 325 217 627 1847 1455 1842 Fragments arranged by size: 1847 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2729 3516 3632 1391 Size: 1338 787 116 4072 Fragments arranged by size: 4072 1338 787 116 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5342 BbvCI CC'TCA_GC Cuts at: 2160 2160 Size: 6313 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4504 4538 1903 Size: 34 2567 34 3678 Fragments arranged by size: 3678 2567 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2545 2977 4205 5415 2545 Size: 432 1228 1210 3443 Fragments arranged by size: 3443 1228 1210 432 BclI T'GATC_A Cuts at: 1548 1548 Size: 6313 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4924 136 Size: 107 122 71 631 605 3252 1525 Fragments arranged by size: 3252 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6308 6308 Size: 6313 BlpI GC'TnA_GC Cuts at: 3730 3730 Size: 6313 Bme1580I G_kGCm'C Cuts at: 1790 3491 4374 5530 1790 Size: 1701 883 1156 2573 Fragments arranged by size: 2573 1701 1156 883 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6313 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2213 2909 3160 Size: 416 53 83 399 24 796 696 251 Cuts at: 3160 3384 4044 5002 442 Size: 224 660 958 1753 Fragments arranged by size: 1753 958 796 696 660 416 399 251 224 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6313 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3057 4973 1239 Size: 1818 1916 2579 Fragments arranged by size: 2579 1916 1818 Bpu10I CC'TnA_GC Cuts at: 2160 3218 2160 Size: 1058 5255 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2669 4438 5303 5601 2669 Size: 1769 865 298 3381 Fragments arranged by size: 3381 1769 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4976 882 Size: 617 3477 2219 Fragments arranged by size: 3477 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3495 4104 493 Size: 711 2291 609 2702 Fragments arranged by size: 2702 2291 711 609 BsaBI GATnn'nnATC Cuts at: 6307 6307 Size: 6313 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3324 4120 4502 5137 Size: 53 83 186 936 1791 796 382 635 Cuts at: 5137 275 Size: 1451 Fragments arranged by size: 1791 1451 936 796 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4746 5144 5418 5565 5976 4746 Size: 398 274 147 411 5083 Fragments arranged by size: 5083 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2087 2117 1178 Size: 30 165 30 684 30 5374 Fragments arranged by size: 5374 684 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2148 3340 1186 Size: 962 1192 4159 Fragments arranged by size: 4159 1192 962 BseYI C'CCAG_C Cuts at: 5516 6012 5516 Size: 496 5817 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2859 1163 Size: 573 1123 4617 Fragments arranged by size: 4617 1123 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3763 4524 5552 1540 Size: 275 186 1762 761 1028 2301 Fragments arranged by size: 2301 1762 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3491 4374 4459 5530 3491 Size: 883 85 1071 4274 Fragments arranged by size: 4274 1071 883 85 BsmI GAATG_Cn' Cuts at: 3824 3917 3824 Size: 93 6220 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2729 3491 3516 3632 4374 4459 5530 Size: 399 939 762 25 116 742 85 1071 Cuts at: 5530 1391 Size: 2174 Fragments arranged by size: 2174 1071 939 762 742 399 116 85 25 BspEI T'CCGG_A Cuts at: 5976 5976 Size: 6313 BspHI T'CATG_A Cuts at: 2065 4202 5932 2065 Size: 2137 1730 2446 Fragments arranged by size: 2446 2137 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3474 3668 844 Size: 2630 194 3489 Fragments arranged by size: 3489 2630 194 BsrBI CCG'CTC Cuts at: 1394 1823 4200 1394 Size: 429 2377 3507 Fragments arranged by size: 3507 2377 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3413 4808 4982 65 Size: 1183 2165 1395 174 1396 Fragments arranged by size: 2165 1396 1395 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4957 5144 1700 Size: 110 3147 187 2869 Fragments arranged by size: 3147 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2485 96 Size: 2389 3924 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6313 BssSI C'ACGA_G Cuts at: 3110 4373 5385 3110 Size: 1263 1012 4038 Fragments arranged by size: 4038 1263 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6313 BstBI TT'CG_AA Cuts at: 2219 5117 2219 Size: 2898 3415 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6313 BstYI r'GATC_y Cuts at: 1972 3275 3385 3643 4010 4395 4412 5864 5950 Size: 1303 110 258 367 385 17 1452 86 Cuts at: 5950 5962 6308 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1303 385 367 346 258 110 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2360 513 Size: 552 939 356 4466 Fragments arranged by size: 4466 939 552 356 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5574 Fragments arranged by size: 5574 683 56 BtsI GCAGTG_nn' Cuts at: 3103 3305 3829 4623 4643 3103 Size: 202 524 794 20 4773 Fragments arranged by size: 4773 794 524 202 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6313 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6278 DraI TTT'AAA Cuts at: 3546 3617 3546 Size: 71 6242 DraIII CAC_nnn'GTG Cuts at: 2997 2997 Size: 6313 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5320 809 Size: 507 4004 1802 Fragments arranged by size: 4004 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3760 4649 6278 Size: 54 1425 50 275 18 1930 889 1629 Cuts at: 6278 8 Size: 43 Fragments arranged by size: 1930 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3760 1537 Size: 275 1948 4090 Fragments arranged by size: 4090 1948 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2063 2329 2973 3251 4243 1353 Size: 710 266 644 278 992 3423 Fragments arranged by size: 3423 992 710 644 278 266 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3251 4900 5274 5420 387 Size: 1064 138 1662 1649 374 146 1280 Fragments arranged by size: 1662 1649 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3057 3222 4376 4973 5760 6028 1239 Size: 393 1425 165 1154 597 787 268 1524 Fragments arranged by size: 1524 1425 1154 787 597 393 268 165 EcoRI G'AATT_C Cuts at: 2224 3622 4000 2224 Size: 1398 378 4537 Fragments arranged by size: 4537 1398 378 EcoRV GAT'ATC Cuts at: 3033 3033 Size: 6313 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2623 2810 2623 Size: 187 6126 FspI TGC'GCA Cuts at: 3231 4819 3231 Size: 1588 4725 HaeII r_GCGC'y Cuts at: 1678 2470 5460 1678 Size: 792 2990 2531 Fragments arranged by size: 2990 2531 792 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2087 2119 2330 2362 3585 Size: 32 135 32 515 32 211 32 1223 Cuts at: 3585 3617 4954 4986 5028 5060 1373 Size: 32 1337 32 42 32 2626 Fragments arranged by size: 2626 1337 1223 515 211 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3657 3903 669 Size: 988 281 1719 246 3079 Fragments arranged by size: 3079 1719 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6313 HpaI GTT'AAC Cuts at: 3903 3903 Size: 6313 KpnI G_GTAC'C Cuts at: 3638 3638 Size: 6313 MfeI C'AATT_G Cuts at: 3912 3912 Size: 6313 MluI A'CGCG_T Cuts at: 6189 6189 Size: 6313 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2130 3567 5427 5611 1305 Size: 825 1437 1860 184 2007 Fragments arranged by size: 2007 1860 1437 825 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4491 Fragments arranged by size: 4491 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3051 4271 4630 4789 518 Size: 648 1885 1220 359 159 2042 Fragments arranged by size: 2042 1885 1220 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3729 4406 5554 5799 1606 Size: 2123 677 1148 245 2120 Fragments arranged by size: 2123 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2360 513 Size: 552 939 356 4466 Fragments arranged by size: 4466 939 552 356 NdeI CA'TA_TG Cuts at: 387 387 Size: 6313 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6313 NotI GC'GGCC_GC Cuts at: 3760 3760 Size: 6313 NspI r_CATG'y Cuts at: 1132 1229 2357 3287 3403 3671 5216 1132 Size: 97 1128 930 116 268 1545 2229 Fragments arranged by size: 2229 1545 1128 930 268 116 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6313 PciI A'CATG_T Cuts at: 5212 5212 Size: 6313 PflMI CCAn_nnn'nTGG Cuts at: 1108 2366 1108 Size: 1258 5055 PfoI T'CCnGG_A Cuts at: 2917 2917 Size: 6313 PmeI GTTT'AAAC Cuts at: 3617 3617 Size: 6313 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4382 4414 5895 5927 4382 Size: 32 1481 32 4768 Fragments arranged by size: 4768 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6313 PsiI TTA'TAA Cuts at: 3381 3561 3883 3381 Size: 180 322 5811 Fragments arranged by size: 5811 322 180 PstI C_TGCA'G Cuts at: 830 3459 3485 3665 830 Size: 2629 26 180 3478 Fragments arranged by size: 3478 2629 180 26 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6313 SalI G'TCGA_C Cuts at: 1936 3655 1936 Size: 1719 4594 SbfI CC_TGCA'GG Cuts at: 3665 3665 Size: 6313 ScaI AGT'ACT Cuts at: 4561 6273 4561 Size: 1712 4601 SfcI C'TryA_G Cuts at: 826 1045 2517 2772 3455 3481 3661 4796 5477 Size: 219 1472 255 683 26 180 1135 681 Cuts at: 5477 5668 826 Size: 191 1471 Fragments arranged by size: 1472 1471 1135 683 681 255 219 191 180 26 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6313 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6313 SmaI CCC'GGG Cuts at: 1872 3640 1872 Size: 1768 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2179 2648 4417 5318 5580 820 Size: 197 926 236 469 1769 901 262 1553 Fragments arranged by size: 1769 1553 926 901 469 262 236 197 SnaBI TAC'GTA Cuts at: 493 3495 4104 493 Size: 3002 609 2702 Fragments arranged by size: 3002 2702 609 SpeI A'CTAG_T Cuts at: 152 152 Size: 6313 SphI G_CATG'C Cuts at: 1132 3287 3671 1132 Size: 2155 384 3774 Fragments arranged by size: 3774 2155 384 SspI AAT'ATT Cuts at: 5 52 2191 4237 5 Size: 47 2139 2046 2081 Fragments arranged by size: 2139 2081 2046 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6313 StyI C'CwwG_G Cuts at: 513 1065 2004 2360 3752 513 Size: 552 939 356 1392 3074 Fragments arranged by size: 3074 1392 939 552 356 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2051 4340 4357 4510 4695 5140 325 Size: 1255 471 2289 17 153 185 445 1498 Fragments arranged by size: 2289 1498 1255 471 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2485 4559 6271 Size: 274 80 33 51 1443 508 2074 1712 Cuts at: 6271 96 Size: 138 Fragments arranged by size: 2074 1712 1443 508 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6273 6305 6273 Size: 32 6281 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6313 XbaI T'CTAG_A Cuts at: 3649 3649 Size: 6313 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 6313 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6313 XmaI C'CCGG_G Cuts at: 1870 3638 1870 Size: 1768 4545 XmnI GAAnn'nnTTC Cuts at: 2033 2057 2338 3183 4442 2033 Size: 24 281 845 1259 3904 Fragments arranged by size: 3904 1259 845 281 24 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4121 276 Size: 53 83 186 936 2587 2468 Fragments arranged by size: 2587 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI