Restriction map for
pFN21AA0520
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# NO CUT ENZYMES ( 63 enzymes) #
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Acc65I AccIII AscI Asp700I Asp718I
BamHI BcgI BciVI BsaBI BseAI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI EcoO65I
EcoT22I FseI HindIII HpaI KpnI
KspI MamI MfeI MroI MunI
NdeI NheI NotI NsiI NspV
PacI PmeI PvuI RcaI RsrII
SacII SbfI ScaI SfiI SfuI
SnaBI SpeI Sse8387I SspBI SspI
SwaI XbaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AatII 2539 GACGTC GACGT^C
AclI 4042 AACGTT AA^CGTT
AflII 1879 CTTAAG C^TTAAG
AgeI 1622 ACCGGT A^CCGGT
AocI 1799 CCTCAGG CC^T[ACGT]AGG
Aor51HI 317 AGCGCT AGC^GCT
AseI 2239 ATTAAT AT^TAAT
AsnI 2239 ATTAAT AT^TAAT
AvrII 1807 CCTAGG C^CTAGG
BalI 1547 TGGCCA TGG^CCA
BbeI 1930 GGCGCC GGCGC^C
BbrPI 3115 CACGTG CAC^GTG
BclI 1406 TGATCA T^GATCA
BfrI 1879 CTTAAG C^TTAAG
BglII 2081 AGATCT A^GATCT
BlnI 1807 CCTAGG C^CTAGG
Bsu36I 1799 CCTCAGG CC^T[ACGT]AGG
DraI 1513 TTTAAA TTT^AAA
DraIII 2179 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3781 GACATCCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 317 AGCGCT AGC^GCT
Eco81I 1799 CCTCAGG CC^T[ACGT]AGG
EcoRI 1065 GAATTC G^AATTC
FbaI 1406 TGATCA T^GATCA
KasI 1930 GGCGCC G^GCGCC
MluI 515 ACGCGT A^CGCGT
MluNI 1547 TGGCCA TGG^CCA
MscI 1547 TGGCCA TGG^CCA
NarI 1930 GGCGCC GG^CGCC
NcoI 3504 CCATGG C^CATGG
NruI 2991 TCGCGA TCG^CGA
PaeR7I 1089 CTCGAG C^TCGAG
PinAI 1622 ACCGGT A^CCGGT
PmaCI 3115 CACGTG CAC^GTG
PmlI 3115 CACGTG CAC^GTG
PshAI 585 GACGCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2239 ATTAAT AT^TAAT
Psp1406I 4042 AACGTT AA^CGTT
SalI 2379 GTCGAC G^TCGAC
SapI 357 GCTCTTC GCTCTTC(1/4)
SexAI 3839 ACCTGGT A^CC[AT]GGT
SfoI 1930 GGCGCC GGC^GCC
SgrAI 3597 CACCGGCG C[GA]^CCGG[CT]G
SmaI 1361 CCCGGG CCC^GGG
XcmI 2555 CCAAGCGCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1089 CTCGAG C^TCGAG
XmaCI 1361 CCCGGG C^CCGGG
XmaI 1361 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AccI 2379 GTCGAC GT^[AC][GT]AC
2638 GTCTAC
AflIII 515 ACGCGT A^C[GA][CT]GT
1523 ACGTGT
AhdI 422 GACTGATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1522 GACGTGTGGTC
ApaI 824 GGGCCC GGGCC^C
3508 GGGCCC
AspEI 422 GACTGATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1522 GACGTGTGGTC
AviII 218 TGCGCA TGC^GCA
567 TGCGCA
BglI 2455 GCCGCCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2927 GCCTCATCGGC
BsaAI 3115 CACGTG [CT]AC^GT[GA]
3336 CACGTA
BsaWI 347 TCCGGT [AT]^CCGG[AT]
1622 ACCGGT
BsmBI 2808 CGTCTC CGTCTC(1/5)
3029 GAGACG
BstAPI 21 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3233 GCAAGGAGTGC
EagI 2699 CGGCCG C^GGCCG
3518 CGGCCG
Eam1105I 422 GACTGATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1522 GACGTGTGGTC
EclXI 2699 CGGCCG C^GGCCG
3518 CGGCCG
Eco52I 2699 CGGCCG C^GGCCG
3518 CGGCCG
EcoRV 1195 GATATC GAT^ATC
1710 GATATC
FspI 218 TGCGCA TGC^GCA
567 TGCGCA
SacI 3259 GAGCTC GAGCT^C
3280 GAGCTC
SphI 1080 GCATGC GCATG^C
3068 GCATGC
StuI 305 AGGCCT AGG^CCT
1754 AGGCCT
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# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI Alw44I AlwI AlwNI ApaLI
ApoI AspHI AspI AvaI AvaII
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV NspI PflFI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XhoII