(Circular) (Six-base) MAPSORT of: pFN21AA0520.seq Check: 1231 from: 1 to: 8764 pFN21AA0520 8764 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4424 5415 4424 Size: 991 7773 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4548 6574 278 Size: 53 83 186 936 3012 2026 2468 Fragments arranged by size: 3012 2468 2026 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4385 4644 6107 1937 Size: 2448 259 1463 4594 Fragments arranged by size: 4594 2448 1463 259 Acc65I G'GTAC_C Cuts at: 6085 6085 Size: 8764 AclI AA'CG_TT Cuts at: 1563 6048 6891 7264 1563 Size: 4485 843 373 3063 Fragments arranged by size: 4485 3063 843 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3948 4342 4445 4459 5156 5188 6827 8211 Size: 2316 394 103 14 697 32 1639 1384 Cuts at: 8211 8479 1632 Size: 268 1917 Fragments arranged by size: 2316 1917 1639 1384 697 394 268 103 32 14 AfeI AGC'GCT Cuts at: 2324 2324 Size: 8764 AflII C'TTAA_G Cuts at: 820 1017 3884 820 Size: 197 2867 5700 Fragments arranged by size: 5700 2867 197 AflIII A'CryG_T Cuts at: 1203 2520 3528 7663 8640 1203 Size: 1317 1008 4135 977 1327 Fragments arranged by size: 4135 1327 1317 1008 977 AgeI A'CCGG_T Cuts at: 3627 7595 3627 Size: 3968 4796 AhdI GACnn_n'nnGTC Cuts at: 2432 3532 7493 2432 Size: 1100 3961 3703 Fragments arranged by size: 3961 3703 1100 AleI CACnn'nnGTG Cuts at: 1166 2051 4781 5606 1166 Size: 885 2730 825 4324 Fragments arranged by size: 4324 2730 885 825 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2102 2708 2876 1184 Size: 918 606 168 7072 Fragments arranged by size: 7072 918 606 168 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2270 2302 2270 Size: 32 8732 AlwNI CAG_nnn'CTG Cuts at: 2029 2032 2271 4279 4755 5416 6507 8079 2029 Size: 3 239 2008 476 661 1091 1572 2714 Fragments arranged by size: 2714 2008 1572 1091 661 476 239 3 ApaI G_GGCC'C Cuts at: 2833 5517 2833 Size: 2684 6080 ApaLI G'TGCA_C Cuts at: 3473 4724 5737 6821 7977 3473 Size: 1251 1013 1084 1156 4260 Fragments arranged by size: 4260 1251 1156 1084 1013 ApoI r'AATT_y Cuts at: 1493 3070 3521 4867 5000 6073 6299 6451 8560 Size: 1577 451 1346 133 1073 226 152 2109 Cuts at: 8560 1493 Size: 1697 Fragments arranged by size: 2109 1697 1577 1346 1073 451 226 152 133 AseI AT'TA_AT Cuts at: 160 1032 4245 7318 160 Size: 872 3213 3073 1606 Fragments arranged by size: 3213 3073 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8764 AvaI C'yCGr_G Cuts at: 1870 1943 3094 3359 3366 6089 1870 Size: 73 1151 265 7 2723 4545 Fragments arranged by size: 4545 2723 1151 265 73 7 AvrII C'CTAG_G Cuts at: 3812 3812 Size: 8764 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2714 2747 2714 Size: 33 8731 BamHI G'GATC_C Cuts at: 6094 6461 6094 Size: 367 8397 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3482 3935 4903 5537 5848 Size: 325 217 627 1695 453 968 634 311 Cuts at: 5848 6085 7540 618 Size: 237 1455 1842 Fragments arranged by size: 1842 1695 1455 968 634 627 453 325 311 237 217 BanII G_rGCy'C Cuts at: 1391 2833 3101 3366 4032 5268 5289 5517 5949 Size: 1442 268 265 666 1236 21 228 432 Cuts at: 5949 6083 1391 Size: 134 4072 Fragments arranged by size: 4072 1442 1236 666 432 268 265 228 134 21 BbeI G_GCGC'C Cuts at: 3939 3939 Size: 8764 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3114 3710 4212 4343 4465 4951 5723 Size: 971 1215 596 502 131 122 486 772 Cuts at: 5723 928 Size: 3969 Fragments arranged by size: 3969 1215 971 772 596 502 486 131 122 BbvCI CC'TCA_GC Cuts at: 4034 4148 5099 4034 Size: 114 951 7699 Fragments arranged by size: 7699 951 114 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6955 6989 1903 Size: 34 5018 34 3678 Fragments arranged by size: 5018 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6656 7866 6656 Size: 1210 7554 BclI T'GATC_A Cuts at: 1548 3411 1548 Size: 1863 6901 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4466 4938 7375 Size: 107 122 71 631 605 2794 472 2437 Cuts at: 7375 136 Size: 1525 Fragments arranged by size: 2794 2437 1525 631 605 472 122 107 71 BglII A'GATC_T Cuts at: 4086 8759 4086 Size: 4673 4091 BlpI GC'TnA_GC Cuts at: 2849 4748 5127 6181 2849 Size: 1899 379 1054 5432 Fragments arranged by size: 5432 1899 1054 379 Bme1580I G_kGCm'C Cuts at: 1790 2833 3477 3485 4728 4826 5517 5741 6825 Size: 1043 644 8 1243 98 691 224 1084 Cuts at: 6825 7981 1790 Size: 1156 2573 Fragments arranged by size: 2573 1243 1156 1084 1043 691 644 224 98 8 BmgBI CAC'GTC Cuts at: 1318 3529 4598 5038 1318 Size: 2211 1069 440 5044 Fragments arranged by size: 5044 2211 1069 440 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2848 4682 6495 Size: 416 53 83 399 24 1431 1834 1813 Cuts at: 6495 7453 442 Size: 958 1753 Fragments arranged by size: 1834 1813 1753 1431 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8764 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2278 2278 Size: 8764 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2050 2248 2324 2339 4020 4301 4514 4559 Size: 811 198 76 15 1681 281 213 45 Cuts at: 4559 4592 7424 1239 Size: 33 2832 2579 Fragments arranged by size: 2832 2579 1681 811 281 213 198 76 45 33 15 Bpu10I CC'TnA_GC Cuts at: 4034 4148 5099 5291 4034 Size: 114 951 192 7507 Fragments arranged by size: 7507 951 192 114 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2646 2921 5671 6889 7754 8052 2646 Size: 275 2750 1218 865 298 3358 Fragments arranged by size: 3358 2750 1218 865 298 275 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2768 3260 3293 4664 5826 5926 7427 Size: 617 1269 492 33 1371 1162 100 1501 Cuts at: 7427 882 Size: 2219 Fragments arranged by size: 2219 1501 1371 1269 1162 617 492 100 33 BsaAI yAC'GTr Cuts at: 493 1204 5122 5343 6555 493 Size: 711 3918 221 1212 2702 Fragments arranged by size: 3918 2702 1212 711 221 BsaBI GATnn'nnATC Cuts at: 8758 8758 Size: 8764 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2190 2591 3936 4179 Size: 53 83 186 936 657 401 1345 243 Cuts at: 4179 4545 5354 6571 6953 7588 275 Size: 366 809 1217 382 635 1451 Fragments arranged by size: 1451 1345 1217 936 809 657 635 401 382 366 243 186 83 53 BsaWI w'CCGG_w Cuts at: 2352 3627 7197 7595 7869 8016 8427 2352 Size: 1275 3570 398 274 147 411 2689 Fragments arranged by size: 3570 2689 1275 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2138 2168 3290 3320 3901 Size: 30 165 30 735 30 1122 30 581 Cuts at: 3901 3931 4432 4462 4487 4517 5559 5589 1178 Size: 30 501 30 25 30 1042 30 4353 Fragments arranged by size: 4353 1122 1042 735 581 501 165 30 30 30 30 30 30 30 30 25 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2146 2321 3074 3311 3526 3960 4022 4064 Size: 960 175 753 237 215 434 62 42 Cuts at: 4064 5234 1186 Size: 1170 4716 Fragments arranged by size: 4716 1170 960 753 434 237 215 175 62 42 BseYI C'CCAG_C Cuts at: 2244 2340 3571 3664 7967 8463 2244 Size: 96 1231 93 4303 496 2545 Fragments arranged by size: 4303 2545 1231 496 96 93 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2819 3774 4500 4540 1163 Size: 573 1083 955 726 40 5387 Fragments arranged by size: 5387 1083 955 726 573 40 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3277 3286 4707 5526 6214 6975 Size: 275 186 1276 9 1421 819 688 761 Cuts at: 6975 8003 1540 Size: 1028 2301 Fragments arranged by size: 2301 1421 1276 1028 819 761 688 275 186 9 BsiHKAI G_wGCw'C Cuts at: 2697 2930 3477 3952 4728 5268 5289 5406 5741 Size: 233 547 475 776 540 21 117 335 Cuts at: 5741 6825 6910 7981 2697 Size: 1084 85 1071 3480 Fragments arranged by size: 3480 1084 1071 776 547 540 475 335 233 117 85 21 BsmI GAATG_Cn' Cuts at: 6275 6368 6275 Size: 93 8671 BsmBI CGTCTCn'nnnn_ Cuts at: 4819 5028 4819 Size: 209 8555 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2697 2833 2930 3101 3366 3477 3485 Size: 399 907 136 97 171 265 111 8 Cuts at: 3485 3952 4032 4728 4826 5268 5289 5406 5517 Size: 467 80 696 98 442 21 117 111 Cuts at: 5517 5741 5949 6083 6825 6910 7981 1391 Size: 224 208 134 742 85 1071 2174 Fragments arranged by size: 2174 1071 907 742 696 467 442 399 265 224 208 171 136 134 117 111 111 98 97 85 80 21 8 BspEI T'CCGG_A Cuts at: 8427 8427 Size: 8764 BspHI T'CATG_A Cuts at: 6653 8383 6653 Size: 1730 7034 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3899 4424 5415 6119 844 Size: 3055 525 991 704 3489 Fragments arranged by size: 3489 3055 991 704 525 BsrBI CCG'CTC Cuts at: 1394 1823 6651 1394 Size: 429 4828 3507 Fragments arranged by size: 4828 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 7259 7433 65 Size: 1183 6011 174 1396 Fragments arranged by size: 6011 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2217 2346 3581 3627 3932 4583 5603 Size: 110 407 129 1235 46 305 651 1020 Cuts at: 5603 7408 7595 1700 Size: 1805 187 2869 Fragments arranged by size: 2869 1805 1235 1020 651 407 305 187 129 110 46 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8764 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8764 BssSI C'ACGA_G Cuts at: 6824 7836 6824 Size: 1012 7752 BstAPI GCAn_nnn'nTGC Cuts at: 794 2032 5244 794 Size: 1238 3212 4314 Fragments arranged by size: 4314 3212 1238 BstBI TT'CG_AA Cuts at: 7568 7568 Size: 8764 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8764 BstXI CCAn_nnnn'nTGG Cuts at: 3562 4525 4783 5347 5593 5608 5804 3562 Size: 963 258 564 246 15 196 6522 Fragments arranged by size: 6522 963 564 258 246 196 15 BstYI r'GATC_y Cuts at: 1972 2420 4086 5047 5064 5412 6094 6461 6846 Size: 448 1666 961 17 348 682 367 385 Cuts at: 6846 6863 8315 8401 8413 8759 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1666 1452 961 682 448 385 367 348 346 86 17 17 12 Bsu36I CC'TnA_GG Cuts at: 3805 3805 Size: 8764 BtgI C'CryG_G Cuts at: 513 1065 2004 2866 5029 5509 5936 513 Size: 552 939 862 2163 480 427 3341 Fragments arranged by size: 3341 2163 939 862 552 480 427 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 5566 487 Size: 683 56 4340 3685 Fragments arranged by size: 4340 3685 683 56 BtsI GCAGTG_nn' Cuts at: 2137 4600 6280 7074 7094 2137 Size: 2463 1680 794 20 3807 Fragments arranged by size: 3807 2463 1680 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8764 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 6042 6077 529 Size: 35 5478 35 3216 Fragments arranged by size: 5478 3216 35 35 DraI TTT'AAA Cuts at: 3520 6068 3520 Size: 2548 6216 DraIII CAC_nnn'GTG Cuts at: 4189 4189 Size: 8764 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5792 7771 809 Size: 507 4476 1979 1802 Fragments arranged by size: 4476 1979 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2628 3552 3579 Size: 54 1425 50 275 18 798 924 27 Cuts at: 3579 4437 4638 4704 4939 5523 6211 7100 8729 Size: 858 201 66 235 584 688 889 1629 Cuts at: 8729 8 Size: 43 Fragments arranged by size: 1629 1425 924 889 858 798 688 584 275 235 201 66 54 50 43 27 18 EagI C'GGCC_G Cuts at: 1537 1812 4704 5523 6211 1537 Size: 275 2892 819 688 4090 Fragments arranged by size: 4090 2892 819 688 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2369 4305 5628 6694 1353 Size: 1016 1936 1323 1066 3423 Fragments arranged by size: 3423 1936 1323 1066 1016 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2226 3140 7351 7725 7871 387 Size: 1064 138 637 914 4211 374 146 1280 Fragments arranged by size: 4211 1280 1064 914 637 374 146 138 EcoICRI GAG'CTC Cuts at: 5266 5287 5266 Size: 21 8743 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2050 2248 2324 2339 3948 4020 4301 Size: 393 418 198 76 15 1609 72 281 Cuts at: 4301 4342 4445 4459 4514 4559 4592 5156 5188 Size: 41 103 14 55 45 33 564 32 Cuts at: 5188 6827 7424 8211 8479 1239 Size: 1639 597 787 268 1524 Fragments arranged by size: 1639 1609 1524 787 597 564 418 393 281 268 198 103 76 72 55 45 41 33 32 15 14 EcoNI CCTnn'n_nnAGG Cuts at: 2306 2598 3293 4271 4759 2306 Size: 292 695 978 488 6311 Fragments arranged by size: 6311 978 695 488 292 EcoO109I rG'GnC_Cy Cuts at: 2259 2278 2829 2830 2968 3809 4004 4764 5254 Size: 19 551 1 138 841 195 760 490 Cuts at: 5254 5514 2259 Size: 260 5509 Fragments arranged by size: 5509 841 760 551 490 260 195 138 19 1 EcoRI G'AATT_C Cuts at: 3070 6073 6451 3070 Size: 3003 378 5383 Fragments arranged by size: 5383 3003 378 EcoRV GAT'ATC Cuts at: 3202 3717 3202 Size: 515 8249 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5066 5066 Size: 8764 FspI TGC'GCA Cuts at: 2225 2574 7270 2225 Size: 349 4696 3719 Fragments arranged by size: 4696 3719 349 HaeII r_GCGC'y Cuts at: 1678 2326 2347 3939 4567 7911 1678 Size: 648 21 1592 628 3344 2531 Fragments arranged by size: 3344 2531 1592 648 628 21 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2418 2450 2728 2760 3518 Size: 32 135 32 846 32 278 32 758 Cuts at: 3518 3550 3706 3738 4288 4320 4487 4519 5166 Size: 32 156 32 550 32 167 32 647 Cuts at: 5166 5198 5559 5591 5861 5893 7405 7437 7479 Size: 32 361 32 270 32 1512 32 42 Cuts at: 7479 7511 1373 Size: 32 2626 Fragments arranged by size: 2626 1512 846 758 647 550 361 278 270 167 156 135 42 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4386 5200 5578 6108 6354 669 Size: 988 281 2448 814 378 530 246 3079 Fragments arranged by size: 3079 2448 988 814 530 378 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8764 HpaI GTT'AAC Cuts at: 6354 6354 Size: 8764 KasI G'GCGC_C Cuts at: 3935 3935 Size: 8764 KpnI G_GTAC'C Cuts at: 6089 6089 Size: 8764 MfeI C'AATT_G Cuts at: 6363 6363 Size: 8764 MluI A'CGCG_T Cuts at: 2520 8640 2520 Size: 6120 2644 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2145 2643 3561 7878 8062 1305 Size: 840 498 918 4317 184 2007 Fragments arranged by size: 4317 2007 918 840 498 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3554 10 Size: 54 1425 343 1722 5220 Fragments arranged by size: 5220 1722 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2051 2690 3218 3395 4523 4619 4781 Size: 648 885 639 528 177 1128 96 162 Cuts at: 4781 5345 5606 5624 6722 7081 7240 518 Size: 564 261 18 1098 359 159 2042 Fragments arranged by size: 2042 1128 1098 885 648 639 564 528 359 261 177 162 159 96 18 MspA1I CmG'CkG Cuts at: 1606 2029 2070 2113 2248 2271 3571 3750 4147 Size: 423 41 43 135 23 1300 179 397 Cuts at: 4147 4798 5134 5172 5770 6180 6857 8005 8250 Size: 651 336 38 598 410 677 1148 245 Cuts at: 8250 1606 Size: 2120 Fragments arranged by size: 2120 1300 1148 677 651 598 423 410 397 336 245 179 135 43 41 38 23 NaeI GCC'GGC Cuts at: 2219 3583 3934 4585 2219 Size: 1364 351 651 6398 Fragments arranged by size: 6398 1364 651 351 NarI GG'CG_CC Cuts at: 3936 3936 Size: 8764 NcoI C'CATG_G Cuts at: 513 1065 2004 5509 513 Size: 552 939 3505 3768 Fragments arranged by size: 3768 3505 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8764 NgoMIV G'CCGG_C Cuts at: 2217 3581 3932 4583 2217 Size: 1364 351 651 6398 Fragments arranged by size: 6398 1364 651 351 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8764 NotI GC'GGCC_GC Cuts at: 6211 6211 Size: 8764 NruI TCG'CGA Cuts at: 4998 4998 Size: 8764 NspI r_CATG'y Cuts at: 1132 1229 3089 5077 5473 6122 7667 1132 Size: 97 1860 1988 396 649 1545 2229 Fragments arranged by size: 2229 1988 1860 1545 649 396 97 PasI CC'CwG_GG Cuts at: 1348 3325 1348 Size: 1977 6787 PciI A'CATG_T Cuts at: 7663 7663 Size: 8764 PflMI CCAn_nnn'nTGG Cuts at: 1108 2271 3247 3676 4189 4948 5866 1108 Size: 1163 976 429 513 759 918 4006 Fragments arranged by size: 4006 1163 976 918 759 513 429 PfoI T'CCnGG_A Cuts at: 2793 2921 4164 4266 4368 4536 4754 2793 Size: 128 1243 102 102 168 218 6803 Fragments arranged by size: 6803 1243 218 168 128 102 102 PmeI GTTT'AAAC Cuts at: 6068 6068 Size: 8764 PmlI CAC'GTG Cuts at: 5122 5122 Size: 8764 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6833 6865 8346 8378 6833 Size: 32 1481 32 7219 Fragments arranged by size: 7219 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2278 2968 4004 4764 5254 2278 Size: 690 1036 760 490 5788 Fragments arranged by size: 5788 1036 760 690 490 PshAI GACnn'nnGTC Cuts at: 1613 2594 1613 Size: 981 7783 PsiI TTA'TAA Cuts at: 6334 6334 Size: 8764 PspOMI G'GGCC_C Cuts at: 2829 5513 2829 Size: 2684 6080 PspXI vC'TCGA_Gb Cuts at: 3094 3094 Size: 8764 PstI C_TGCA'G Cuts at: 830 2168 3749 5412 6116 830 Size: 1338 1581 1663 704 3478 Fragments arranged by size: 3478 1663 1581 1338 704 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8764 PvuII CAG'CTG Cuts at: 2029 2113 2248 3571 3750 5134 5770 2029 Size: 84 135 1323 179 1384 636 5023 Fragments arranged by size: 5023 1384 1323 636 179 135 84 SacI G_AGCT'C Cuts at: 5268 5289 5268 Size: 21 8743 SalI G'TCGA_C Cuts at: 1936 4384 6106 1936 Size: 2448 1722 4594 Fragments arranged by size: 4594 2448 1722 SanDI GG'GwC_CC Cuts at: 2278 2278 Size: 8764 SapI GCTCTTCn'nnn_ Cuts at: 2369 2369 Size: 8764 SbfI CC_TGCA'GG Cuts at: 6116 6116 Size: 8764 ScaI AGT'ACT Cuts at: 7012 8724 7012 Size: 1712 7052 SexAI A'CCwGG_T Cuts at: 5844 5844 Size: 8764 SfcI C'TryA_G Cuts at: 826 1045 2164 3141 3745 4291 5305 5408 5677 Size: 219 1119 977 604 546 1014 103 269 Cuts at: 5677 6112 7247 7928 8119 826 Size: 435 1135 681 191 1471 Fragments arranged by size: 1471 1135 1119 1014 977 681 604 546 435 269 219 191 103 SfoI GGC'GCC Cuts at: 3937 3937 Size: 8764 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8764 SgrAI Cr'CCGG_yG Cuts at: 1700 5603 1700 Size: 3903 4861 SmaI CCC'GGG Cuts at: 1872 3368 6091 1872 Size: 1496 2723 4545 Fragments arranged by size: 4545 2723 1496 SmlI C'TyrA_G Cuts at: 820 1017 1943 2661 2936 3094 3884 5650 6868 Size: 197 926 718 275 158 790 1766 1218 Cuts at: 6868 7769 8031 820 Size: 901 262 1553 Fragments arranged by size: 1766 1553 1218 926 901 790 718 275 262 197 158 SnaBI TAC'GTA Cuts at: 493 6555 493 Size: 6062 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8764 SphI G_CATG'C Cuts at: 1132 3089 5077 6122 1132 Size: 1957 1988 1045 3774 Fragments arranged by size: 3774 1988 1957 1045 SspI AAT'ATT Cuts at: 5 52 6688 5 Size: 47 6636 2081 Fragments arranged by size: 6636 2081 47 StuI AGG'CCT Cuts at: 1517 2312 3761 1517 Size: 795 1449 6520 Fragments arranged by size: 6520 1449 795 StyI C'CwwG_G Cuts at: 513 1065 2004 3732 3812 3990 5430 5509 5907 Size: 552 939 1728 80 178 1440 79 398 Cuts at: 5907 6203 513 Size: 296 3074 Fragments arranged by size: 3074 1728 1440 939 552 398 296 178 80 79 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3263 6791 6808 6961 7146 7591 325 Size: 1255 1683 3528 17 153 185 445 1498 Fragments arranged by size: 3528 1683 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4861 7010 8722 Size: 274 80 33 51 1443 2884 2149 1712 Cuts at: 8722 96 Size: 138 Fragments arranged by size: 2884 2149 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2405 2437 3290 3322 3832 3864 3901 3933 4432 Size: 32 853 32 510 32 37 32 499 Cuts at: 4432 4464 5756 5788 8724 8756 2405 Size: 32 1292 32 2936 32 2413 Fragments arranged by size: 2936 2413 1292 853 510 499 37 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3829 4405 5573 1316 Size: 2513 576 1168 4507 Fragments arranged by size: 4507 2513 1168 576 XbaI T'CTAG_A Cuts at: 6100 6100 Size: 8764 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4567 4567 Size: 8764 XhoI C'TCGA_G Cuts at: 1943 3094 1943 Size: 1151 7613 XmaI C'CCGG_G Cuts at: 1870 3366 6089 1870 Size: 1496 2723 4545 Fragments arranged by size: 4545 2723 1496 XmnI GAAnn'nnTTC Cuts at: 6893 6893 Size: 8764 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4546 6572 276 Size: 53 83 186 936 3012 2026 2468 Fragments arranged by size: 3012 2468 2026 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI BfrBI BsiWI BstZ17I FseI FspAI NsiI PacI PsrI RsrII SacII SfiI SrfI SwaI