Restriction map for
pFN21AA0520
(Linear) (Six-base) MAPSORT of: KIAA0520.seq Check: 5100 from: 1 to: 4059
KIAA0520.orf
With 182 enzymes: SgfI *
July 18, 2007 13:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2419 3410 4059
Size: 2419 991 649
Fragments arranged by size:
2419 991 649
AatII G_ACGT'C
Cuts at: 0 2543 4059
Size: 2543 1516
AccI GT'mk_AC
Cuts at: 0 2380 2639 4059
Size: 2380 259 1420
Fragments arranged by size:
2380 1420 259
AclI AA'CG_TT
Cuts at: 0 4043 4059
Size: 4043 16
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1943 2337 2440 2454 3151 3183 4059
Size: 1943 394 103 14 697 32 876
Fragments arranged by size:
1943 876 697 394 103 32 14
AfeI AGC'GCT
Cuts at: 0 319 4059
Size: 319 3740
AflII C'TTAA_G
Cuts at: 0 1879 4059
Size: 1879 2180
AflIII A'CryG_T
Cuts at: 0 515 1523 4059
Size: 515 1008 2536
Fragments arranged by size:
2536 1008 515
AgeI A'CCGG_T
Cuts at: 0 1622 4059
Size: 1622 2437
AhdI GACnn_n'nnGTC
Cuts at: 0 427 1527 4059
Size: 427 1100 2532
Fragments arranged by size:
2532 1100 427
AleI CACnn'nnGTG
Cuts at: 0 46 2776 3601 4059
Size: 46 2730 825 458
Fragments arranged by size:
2730 825 458 46
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 97 703 871 4059
Size: 97 606 168 3188
Fragments arranged by size:
3188 606 168 97
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 265 297 4059
Size: 265 32 3762
Fragments arranged by size:
3762 265 32
AlwNI CAG_nnn'CTG
Cuts at: 0 24 27 266 2274 2750 3411 4059
Size: 24 3 239 2008 476 661 648
Fragments arranged by size:
2008 661 648 476 239 24 3
ApaI G_GGCC'C
Cuts at: 0 828 3512 4059
Size: 828 2684 547
Fragments arranged by size:
2684 828 547
ApaLI G'TGCA_C
Cuts at: 0 1468 2719 3732 4059
Size: 1468 1251 1013 327
Fragments arranged by size:
1468 1251 1013 327
ApoI r'AATT_y
Cuts at: 0 1065 1516 2862 2995 4059
Size: 1065 451 1346 133 1064
Fragments arranged by size:
1346 1065 1064 451 133
AseI AT'TA_AT
Cuts at: 0 2240 4059
Size: 2240 1819
AvaI C'yCGr_G
Cuts at: 0 1089 1354 1361 4059
Size: 1089 265 7 2698
Fragments arranged by size:
2698 1089 265 7
AvrII C'CTAG_G
Cuts at: 0 1807 4059
Size: 1807 2252
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 709 742 4059
Size: 709 33 3317
Fragments arranged by size:
3317 709 33
BanI G'GyrC_C
Cuts at: 0 1477 1930 2898 3532 3843 4059
Size: 1477 453 968 634 311 216
Fragments arranged by size:
1477 968 634 453 311 216
BanII G_rGCy'C
Cuts at: 0 828 1096 1361 2027 3263 3284 3512 3944
Size: 828 268 265 666 1236 21 228 432
Cuts at: 3944 4059
Size: 115
Fragments arranged by size:
1236 828 666 432 268 265 228 115
21
BbeI G_GCGC'C
Cuts at: 0 1934 4059
Size: 1934 2125
BbsI GAAGACnn'nnnn_
Cuts at: 0 1109 1705 2207 2338 2460 2946 3718 4059
Size: 1109 596 502 131 122 486 772 341
Fragments arranged by size:
1109 772 596 502 486 341 131 122
BbvCI CC'TCA_GC
Cuts at: 0 2029 2143 3094 4059
Size: 2029 114 951 965
Fragments arranged by size:
2029 965 951 114
BclI T'GATC_A
Cuts at: 0 1406 4059
Size: 1406 2653
BglI GCCn_nnn'nGGC
Cuts at: 0 2461 2933 4059
Size: 2461 472 1126
Fragments arranged by size:
2461 1126 472
BglII A'GATC_T
Cuts at: 0 2081 4059
Size: 2081 1978
BlpI GC'TnA_GC
Cuts at: 0 844 2743 3122 4059
Size: 844 1899 379 937
Fragments arranged by size:
1899 937 844 379
Bme1580I G_kGCm'C
Cuts at: 0 828 1472 1480 2723 2821 3512 3736 4059
Size: 828 644 8 1243 98 691 224 323
Fragments arranged by size:
1243 828 691 644 323 224 98 8
BmgBI CAC'GTC
Cuts at: 0 1524 2593 3033 4059
Size: 1524 1069 440 1026
Fragments arranged by size:
1524 1069 1026 440
BmrI ACTGGGnnnn_n'
Cuts at: 0 843 2677 4059
Size: 843 1834 1382
Fragments arranged by size:
1834 1382 843
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 273 4059
Size: 273 3786
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 45 243 319 334 2015 2296 2509 2554
Size: 45 198 76 15 1681 281 213 45
Cuts at: 2554 2587 4059
Size: 33 1472
Fragments arranged by size:
1681 1472 281 213 198 76 45 45
33 15
Bpu10I CC'TnA_GC
Cuts at: 0 2029 2143 3094 3286 4059
Size: 2029 114 951 192 773
Fragments arranged by size:
2029 951 773 192 114
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 641 916 3666 4059
Size: 641 275 2750 393
Fragments arranged by size:
2750 641 393 275
BsaI GGTCTCn'nnnn_
Cuts at: 0 763 1255 1288 2659 3821 3921 4059
Size: 763 492 33 1371 1162 100 138
Fragments arranged by size:
1371 1162 763 492 138 100 33
BsaAI yAC'GTr
Cuts at: 0 3117 3338 4059
Size: 3117 221 721
Fragments arranged by size:
3117 721 221
BsaHI Gr'CG_yC
Cuts at: 0 185 586 1931 2174 2540 3349 4059
Size: 185 401 1345 243 366 809 710
Fragments arranged by size:
1345 809 710 401 366 243 185
BsaWI w'CCGG_w
Cuts at: 0 347 1622 4059
Size: 347 1275 2437
Fragments arranged by size:
2437 1275 347
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 133 163 1285 1315 1896 1926 2427 2457
Size: 133 30 1122 30 581 30 501 30
Cuts at: 2457 2482 2512 3554 3584 4059
Size: 25 30 1042 30 475
Fragments arranged by size:
1122 1042 581 501 475 133 30 30
30 30 30 30 25
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 141 316 1069 1306 1521 1955 2017 2059
Size: 141 175 753 237 215 434 62 42
Cuts at: 2059 3229 4059
Size: 1170 830
Fragments arranged by size:
1170 830 753 434 237 215 175 141
62 42
BseYI C'CCAG_C
Cuts at: 0 239 335 1566 1659 4059
Size: 239 96 1231 93 2400
Fragments arranged by size:
2400 1231 239 96 93
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 814 1769 2495 2535 4059
Size: 814 955 726 40 1524
Fragments arranged by size:
1524 955 814 726 40
BsiEI CG_ry'CG
Cuts at: 0 1272 1281 2702 3521 4059
Size: 1272 9 1421 819 538
Fragments arranged by size:
1421 1272 819 538 9
BsiHKAI G_wGCw'C
Cuts at: 0 692 925 1472 1947 2723 3263 3284 3401
Size: 692 233 547 475 776 540 21 117
Cuts at: 3401 3736 4059
Size: 335 323
Fragments arranged by size:
776 692 547 540 475 335 323 233
117 21
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2814 3023 4059
Size: 2814 209 1036
Fragments arranged by size:
2814 1036 209
Bsp1286I G_dGCh'C
Cuts at: 0 692 828 925 1096 1361 1472 1480 1947
Size: 692 136 97 171 265 111 8 467
Cuts at: 1947 2027 2723 2821 3263 3284 3401 3512 3736
Size: 80 696 98 442 21 117 111 224
Cuts at: 3736 3944 4059
Size: 208 115
Fragments arranged by size:
696 692 467 442 265 224 208 171
136 117 115 111 111 98 97 80
21 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1894 2419 3410 4059
Size: 1894 525 991 649
Fragments arranged by size:
1894 991 649 525
BsrFI r'CCGG_y
Cuts at: 0 212 341 1576 1622 1927 2578 3598 4059
Size: 212 129 1235 46 305 651 1020 461
Fragments arranged by size:
1235 1020 651 461 305 212 129 46
BstAPI GCAn_nnn'nTGC
Cuts at: 0 27 3239 4059
Size: 27 3212 820
Fragments arranged by size:
3212 820 27
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1557 2520 2778 3342 3588 3603 3799 4059
Size: 1557 963 258 564 246 15 196 260
Fragments arranged by size:
1557 963 564 260 258 246 196 15
BstYI r'GATC_y
Cuts at: 0 415 2081 3042 3059 3407 4059
Size: 415 1666 961 17 348 652
Fragments arranged by size:
1666 961 652 415 348 17
Bsu36I CC'TnA_GG
Cuts at: 0 1800 4059
Size: 1800 2259
BtgI C'CryG_G
Cuts at: 0 861 3024 3504 3931 4059
Size: 861 2163 480 427 128
Fragments arranged by size:
2163 861 480 427 128
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3561 4059
Size: 3561 498
BtsI GCAGTG_nn'
Cuts at: 0 132 2595 4059
Size: 132 2463 1464
Fragments arranged by size:
2463 1464 132
DraI TTT'AAA
Cuts at: 0 1515 4059
Size: 1515 2544
DraIII CAC_nnn'GTG
Cuts at: 0 2184 4059
Size: 2184 1875
DrdI GACnn_nn'nnGTC
Cuts at: 0 3787 4059
Size: 3787 272
EaeI y'GGCC_r
Cuts at: 0 623 1547 1574 2432 2633 2699 2934 3518
Size: 623 924 27 858 201 66 235 584
Cuts at: 3518 4059
Size: 541
Fragments arranged by size:
924 858 623 584 541 235 201 66
27
EagI C'GGCC_G
Cuts at: 0 2699 3518 4059
Size: 2699 819 541
Fragments arranged by size:
2699 819 541
EarI CTCTTCn'nnn_
Cuts at: 0 364 2300 3623 4059
Size: 364 1936 1323 436
Fragments arranged by size:
1936 1323 436 364
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 221 1135 4059
Size: 221 914 2924
Fragments arranged by size:
2924 914 221
EcoICRI GAG'CTC
Cuts at: 0 3261 3282 4059
Size: 3261 21 777
Fragments arranged by size:
3261 777 21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 45 243 319 334 1943 2015 2296 2337
Size: 45 198 76 15 1609 72 281 41
Cuts at: 2337 2440 2454 2509 2554 2587 3151 3183 4059
Size: 103 14 55 45 33 564 32 876
Fragments arranged by size:
1609 876 564 281 198 103 76 72
55 45 45 41 33 32 15 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 301 593 1288 2266 2754 4059
Size: 301 292 695 978 488 1305
Fragments arranged by size:
1305 978 695 488 301 292
EcoO109I rG'GnC_Cy
Cuts at: 0 254 273 824 825 963 1804 1999 2759
Size: 254 19 551 1 138 841 195 760
Cuts at: 2759 3249 3509 4059
Size: 490 260 550
Fragments arranged by size:
841 760 551 550 490 260 254 195
138 19 1
EcoRI G'AATT_C
Cuts at: 0 1065 4059
Size: 1065 2994
EcoRV GAT'ATC
Cuts at: 0 1197 1712 4059
Size: 1197 515 2347
Fragments arranged by size:
2347 1197 515
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3061 4059
Size: 3061 998
FspI TGC'GCA
Cuts at: 0 220 569 4059
Size: 220 349 3490
Fragments arranged by size:
3490 349 220
HaeII r_GCGC'y
Cuts at: 0 321 342 1934 2562 4059
Size: 321 21 1592 628 1497
Fragments arranged by size:
1592 1497 628 321 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 413 445 723 755 1513 1545 1701 1733
Size: 413 32 278 32 758 32 156 32
Cuts at: 1733 2283 2315 2482 2514 3161 3193 3554 3586
Size: 550 32 167 32 647 32 361 32
Cuts at: 3586 3856 3888 4059
Size: 270 32 171
Fragments arranged by size:
758 647 550 413 361 278 270 171
167 156 32 32 32 32 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 2381 3195 3573 4059
Size: 2381 814 378 486
Fragments arranged by size:
2381 814 486 378
KasI G'GCGC_C
Cuts at: 0 1930 4059
Size: 1930 2129
MluI A'CGCG_T
Cuts at: 0 515 4059
Size: 515 3544
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 140 638 1556 4059
Size: 140 498 918 2503
Fragments arranged by size:
2503 918 498 140
MscI TGG'CCA
Cuts at: 0 1549 4059
Size: 1549 2510
MslI CAynn'nnrTG
Cuts at: 0 46 685 1213 1390 2518 2614 2776 3340
Size: 46 639 528 177 1128 96 162 564
Cuts at: 3340 3601 3619 4059
Size: 261 18 440
Fragments arranged by size:
1128 639 564 528 440 261 177 162
96 46 18
MspA1I CmG'CkG
Cuts at: 0 24 65 108 243 266 1566 1745 2142
Size: 24 41 43 135 23 1300 179 397
Cuts at: 2142 2793 3129 3167 3765 4059
Size: 651 336 38 598 294
Fragments arranged by size:
1300 651 598 397 336 294 179 135
43 41 38 24 23
NaeI GCC'GGC
Cuts at: 0 214 1578 1929 2580 4059
Size: 214 1364 351 651 1479
Fragments arranged by size:
1479 1364 651 351 214
NarI GG'CG_CC
Cuts at: 0 1931 4059
Size: 1931 2128
NcoI C'CATG_G
Cuts at: 0 3504 4059
Size: 3504 555
NgoMIV G'CCGG_C
Cuts at: 0 212 1576 1927 2578 4059
Size: 212 1364 351 651 1481
Fragments arranged by size:
1481 1364 651 351 212
NruI TCG'CGA
Cuts at: 0 2993 4059
Size: 2993 1066
NspI r_CATG'y
Cuts at: 0 1084 3072 3468 4059
Size: 1084 1988 396 591
Fragments arranged by size:
1988 1084 591 396
PasI CC'CwG_GG
Cuts at: 0 1320 4059
Size: 1320 2739
PflMI CCAn_nnn'nTGG
Cuts at: 0 266 1242 1671 2184 2943 3861 4059
Size: 266 976 429 513 759 918 198
Fragments arranged by size:
976 918 759 513 429 266 198
PfoI T'CCnGG_A
Cuts at: 0 788 916 2159 2261 2363 2531 2749 4059
Size: 788 128 1243 102 102 168 218 1310
Fragments arranged by size:
1310 1243 788 218 168 128 102 102
PmlI CAC'GTG
Cuts at: 0 3117 4059
Size: 3117 942
PpuMI rG'GwC_Cy
Cuts at: 0 273 963 1999 2759 3249 4059
Size: 273 690 1036 760 490 810
Fragments arranged by size:
1036 810 760 690 490 273
PshAI GACnn'nnGTC
Cuts at: 0 589 4059
Size: 589 3470
PspOMI G'GGCC_C
Cuts at: 0 824 3508 4059
Size: 824 2684 551
Fragments arranged by size:
2684 824 551
PspXI vC'TCGA_Gb
Cuts at: 0 1089 4059
Size: 1089 2970
PstI C_TGCA'G
Cuts at: 0 163 1744 3407 4059
Size: 163 1581 1663 652
Fragments arranged by size:
1663 1581 652 163
PvuII CAG'CTG
Cuts at: 0 24 108 243 1566 1745 3129 3765 4059
Size: 24 84 135 1323 179 1384 636 294
Fragments arranged by size:
1384 1323 636 294 179 135 84 24
SacI G_AGCT'C
Cuts at: 0 3263 3284 4059
Size: 3263 21 775
Fragments arranged by size:
3263 775 21
SalI G'TCGA_C
Cuts at: 0 2379 4059
Size: 2379 1680
SanDI GG'GwC_CC
Cuts at: 0 273 4059
Size: 273 3786
SapI GCTCTTCn'nnn_
Cuts at: 0 364 4059
Size: 364 3695
SexAI A'CCwGG_T
Cuts at: 0 3839 4059
Size: 3839 220
SfcI C'TryA_G
Cuts at: 0 159 1136 1740 2286 3300 3403 3672 4059
Size: 159 977 604 546 1014 103 269 387
Fragments arranged by size:
1014 977 604 546 387 269 159 103
SfoI GGC'GCC
Cuts at: 0 1932 4059
Size: 1932 2127
SgrAI Cr'CCGG_yG
Cuts at: 0 3598 4059
Size: 3598 461
SmaI CCC'GGG
Cuts at: 0 1363 4059
Size: 1363 2696
SmlI C'TyrA_G
Cuts at: 0 656 931 1089 1879 3645 4059
Size: 656 275 158 790 1766 414
Fragments arranged by size:
1766 790 656 414 275 158
SphI G_CATG'C
Cuts at: 0 1084 3072 4059
Size: 1084 1988 987
Fragments arranged by size:
1988 1084 987
StuI AGG'CCT
Cuts at: 0 307 1756 4059
Size: 307 1449 2303
Fragments arranged by size:
2303 1449 307
StyI C'CwwG_G
Cuts at: 0 1727 1807 1985 3425 3504 3902 4059
Size: 1727 80 178 1440 79 398 157
Fragments arranged by size:
1727 1440 398 178 157 80 79
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1258 4059
Size: 1258 2801
TatI w'GTAC_w
Cuts at: 0 2856 4059
Size: 2856 1203
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 400 432 1285 1317 1827 1859 1896 1928
Size: 400 32 853 32 510 32 37 32
Cuts at: 1928 2427 2459 3751 3783 4059
Size: 499 32 1292 32 276
Fragments arranged by size:
1292 853 510 499 400 276 37 32
32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1824 2400 3568 4059
Size: 1824 576 1168 491
Fragments arranged by size:
1824 1168 576 491
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2562 4059
Size: 2562 1497
XhoI C'TCGA_G
Cuts at: 0 1089 4059
Size: 1089 2970
XmaI C'CCGG_G
Cuts at: 0 1361 4059
Size: 1361 2698
ZraI GAC'GTC
Cuts at: 0 2541 4059
Size: 2541 1518
Enzymes that do cut:
AarI AatII AccI AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AvaI AvrII BaeI BanI BanII BbeI
BbsI BbvCI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspMI BsrFI BstAPI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NruI NspI
PasI PflMI PfoI PmlI PpuMI PshAI PspOMI PspXI
PstI PvuII SacI SalI SanDI SapI SexAI SfcI
SfoI SgrAI SmaI SmlI SphI StuI StyI TaqII
TatI TstI Tth111I XcmI XhoI XmaI ZraI
Enzymes that do not cut:
Acc65I AscI AsiSI BamHI BcgI BciVI BfrBI BmtI
BsaBI BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrGI
BssHII BssSI BstBI BstEII BstZ17I ClaI CspCI FseI
FspAI HindIII HpaI KpnI MfeI NdeI NheI NotI
NsiI PacI PciI PmeI PpiI PsiI PsrI PvuI
RsrII SacII SbfI ScaI SfiI SgfI SnaBI SpeI
SrfI SspI SwaI XbaI XmnI