(Circular) (Six-base) MAPSORT of: pFN21AF0405.seq Check: 8545 from: 1 to: 5911 pFN21AF0405 5911 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2582 2582 Size: 5911 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3721 278 Size: 53 83 186 936 2185 2468 Fragments arranged by size: 2468 2185 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2373 3254 1937 Size: 436 881 4594 Fragments arranged by size: 4594 881 436 Acc65I G'GTAC_C Cuts at: 3232 3232 Size: 5911 AclI AA'CG_TT Cuts at: 1563 2101 4038 4411 1563 Size: 538 1937 373 3063 Fragments arranged by size: 3063 1937 538 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2292 2329 2565 2645 3974 5358 5626 1632 Size: 660 37 236 80 1329 1384 268 1917 Fragments arranged by size: 1917 1384 1329 660 268 236 80 37 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5714 AflIII A'CryG_T Cuts at: 1203 4810 5787 1203 Size: 3607 977 1327 Fragments arranged by size: 3607 1327 977 AgeI A'CCGG_T Cuts at: 4742 4742 Size: 5911 AhdI GACnn_n'nnGTC Cuts at: 4640 4640 Size: 5911 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5911 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5911 AlwNI CAG_nnn'CTG Cuts at: 2623 3654 5226 2623 Size: 1031 1572 3308 Fragments arranged by size: 3308 1572 1031 ApaLI G'TGCA_C Cuts at: 2381 3968 5124 2381 Size: 1587 1156 3168 Fragments arranged by size: 3168 1587 1156 ApoI r'AATT_y Cuts at: 1493 2725 3220 3446 3598 5707 1493 Size: 1232 495 226 152 2109 1697 Fragments arranged by size: 2109 1697 1232 495 226 152 AseI AT'TA_AT Cuts at: 160 1032 4465 160 Size: 872 3433 1606 Fragments arranged by size: 3433 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5911 AvaI C'yCGr_G Cuts at: 1870 1943 3236 1870 Size: 73 1293 4545 Fragments arranged by size: 4545 1293 73 BamHI G'GATC_C Cuts at: 3241 3608 3241 Size: 367 5544 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3232 4687 618 Size: 325 217 627 1445 1455 1842 Fragments arranged by size: 1842 1455 1445 627 325 217 BanII G_rGCy'C Cuts at: 1391 2015 3230 1391 Size: 624 1215 4072 Fragments arranged by size: 4072 1215 624 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2535 3006 928 Size: 971 636 471 3833 Fragments arranged by size: 3833 971 636 471 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2341 2375 4102 4136 1903 Size: 34 404 34 1727 34 3678 Fragments arranged by size: 3678 1727 404 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3803 5013 3803 Size: 1210 4701 BclI T'GATC_A Cuts at: 1548 1548 Size: 5911 BfrBI ATG'CAT Cuts at: 2195 2954 2195 Size: 759 5152 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2804 4522 136 Size: 107 122 71 631 605 1132 1718 1525 Fragments arranged by size: 1718 1525 1132 631 605 122 107 71 BglII A'GATC_T Cuts at: 5906 5906 Size: 5911 BlpI GC'TnA_GC Cuts at: 3328 3328 Size: 5911 Bme1580I G_kGCm'C Cuts at: 1790 2385 3972 5128 1790 Size: 595 1587 1156 2573 Fragments arranged by size: 2573 1587 1156 595 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5911 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3642 4600 442 Size: 416 53 83 399 24 2225 958 1753 Fragments arranged by size: 2225 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5911 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2133 2133 Size: 5911 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2482 2489 4571 1239 Size: 1243 7 2082 2579 Fragments arranged by size: 2579 2082 1243 7 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4036 4901 5199 4036 Size: 865 298 4748 Fragments arranged by size: 4748 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4574 882 Size: 617 3075 2219 Fragments arranged by size: 3075 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2734 2803 3702 493 Size: 711 1530 69 899 2702 Fragments arranged by size: 2702 1530 899 711 69 BsaBI GATnn'nnATC Cuts at: 5905 5905 Size: 5911 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3718 4100 4735 275 Size: 53 83 186 936 2185 382 635 1451 Fragments arranged by size: 2185 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4344 4742 5016 5163 5574 4344 Size: 398 274 147 411 4681 Fragments arranged by size: 4681 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2749 2779 2849 2879 1178 Size: 30 165 30 1346 30 70 30 4210 Fragments arranged by size: 4210 1346 165 70 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2751 2754 2757 1186 Size: 1565 3 3 4340 Fragments arranged by size: 4340 1565 3 3 BseYI C'CCAG_C Cuts at: 2282 3197 5114 5610 2282 Size: 915 1917 496 2583 Fragments arranged by size: 2583 1917 915 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2439 1163 Size: 573 703 4635 Fragments arranged by size: 4635 703 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3361 4122 5150 1540 Size: 275 186 1360 761 1028 2301 Fragments arranged by size: 2301 1360 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2385 3972 4057 5128 2385 Size: 1587 85 1071 3168 Fragments arranged by size: 3168 1587 1071 85 BsmI GAATG_Cn' Cuts at: 3143 3422 3515 3143 Size: 279 93 5539 Fragments arranged by size: 5539 279 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2046 2046 Size: 5911 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2015 2385 3230 3972 4057 5128 1391 Size: 399 225 370 845 742 85 1071 2174 Fragments arranged by size: 2174 1071 845 742 399 370 225 85 BspEI T'CCGG_A Cuts at: 5574 5574 Size: 5911 BspHI T'CATG_A Cuts at: 2770 3800 5530 2770 Size: 1030 1730 3151 Fragments arranged by size: 3151 1730 1030 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2231 2582 3266 844 Size: 1387 351 684 3489 Fragments arranged by size: 3489 1387 684 351 BsrBI CCG'CTC Cuts at: 1394 1823 3798 1394 Size: 429 1975 3507 Fragments arranged by size: 3507 1975 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4406 4580 65 Size: 1183 3158 174 1396 Fragments arranged by size: 3158 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4555 4742 1700 Size: 110 2745 187 2869 Fragments arranged by size: 2869 2745 187 110 BsrGI T'GTAC_A Cuts at: 96 3031 96 Size: 2935 2976 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5911 BssSI C'ACGA_G Cuts at: 3971 4983 3971 Size: 1012 4899 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5911 BstBI TT'CG_AA Cuts at: 2242 4715 2242 Size: 2473 3438 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5911 BstXI CCAn_nnnn'nTGG Cuts at: 2681 2681 Size: 5911 BstYI r'GATC_y Cuts at: 1972 2493 3241 3608 3993 4010 5462 5548 5560 Size: 521 748 367 385 17 1452 86 12 Cuts at: 5560 5906 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 748 521 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3019 513 Size: 552 1954 3405 Fragments arranged by size: 3405 1954 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3140 487 Size: 683 56 1914 3258 Fragments arranged by size: 3258 1914 683 56 BtsI GCAGTG_nn' Cuts at: 2427 3427 4221 4241 2427 Size: 1000 794 20 4097 Fragments arranged by size: 4097 1000 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5911 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5876 DraI TTT'AAA Cuts at: 2413 2635 3215 2413 Size: 222 580 5109 Fragments arranged by size: 5109 580 222 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4918 809 Size: 507 3602 1802 Fragments arranged by size: 3602 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3022 3358 4247 Size: 54 1425 50 275 18 1192 336 889 Cuts at: 4247 5876 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1192 889 336 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3358 1537 Size: 275 1546 4090 Fragments arranged by size: 4090 1546 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2271 2348 3841 1353 Size: 918 77 1493 3423 Fragments arranged by size: 3423 1493 918 77 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4498 4872 5018 387 Size: 1064 138 2909 374 146 1280 Fragments arranged by size: 2909 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2292 2329 2482 2489 2565 2645 3974 Size: 393 660 37 153 7 76 80 1329 Cuts at: 3974 4571 5358 5626 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1329 787 660 597 393 268 153 80 76 37 7 EcoRI G'AATT_C Cuts at: 3220 3598 3220 Size: 378 5533 FspI TGC'GCA Cuts at: 4417 4417 Size: 5911 HaeII r_GCGC'y Cuts at: 1678 5058 1678 Size: 3380 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2849 2881 4552 4584 4626 Size: 32 135 32 1277 32 1671 32 42 Cuts at: 4626 4658 1373 Size: 32 2626 Fragments arranged by size: 2626 1671 1277 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3255 3501 669 Size: 988 281 1317 246 3079 Fragments arranged by size: 3079 1317 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5911 HpaI GTT'AAC Cuts at: 3501 3501 Size: 5911 KpnI G_GTAC'C Cuts at: 3236 3236 Size: 5911 MfeI C'AATT_G Cuts at: 3510 3510 Size: 5911 MluI A'CGCG_T Cuts at: 5787 5787 Size: 5911 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2662 5025 5209 1305 Size: 1357 2363 184 2007 Fragments arranged by size: 2363 2007 1357 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4089 Fragments arranged by size: 4089 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3869 4228 4387 518 Size: 648 2703 359 159 2042 Fragments arranged by size: 2703 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2439 3201 3327 4004 5152 5397 1606 Size: 833 762 126 677 1148 245 2120 Fragments arranged by size: 2120 1148 833 762 677 245 126 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5359 NdeI CA'TA_TG Cuts at: 387 387 Size: 5911 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5911 NotI GC'GGCC_GC Cuts at: 3358 3358 Size: 5911 NruI TCG'CGA Cuts at: 3159 3159 Size: 5911 NsiI A_TGCA'T Cuts at: 2197 2956 2197 Size: 759 5152 NspI r_CATG'y Cuts at: 1132 1229 3269 4814 1132 Size: 97 2040 1545 2229 Fragments arranged by size: 2229 2040 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5911 PciI A'CATG_T Cuts at: 4810 4810 Size: 5911 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5911 PfoI T'CCnGG_A Cuts at: 2957 2957 Size: 5911 PmeI GTTT'AAAC Cuts at: 3215 3215 Size: 5911 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2270 2302 3980 4012 5493 5525 2270 Size: 32 1678 32 1481 32 2656 Fragments arranged by size: 2656 1678 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5911 PsiI TTA'TAA Cuts at: 3481 3481 Size: 5911 PstI C_TGCA'G Cuts at: 830 3263 830 Size: 2433 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5911 PvuII CAG'CTG Cuts at: 2439 3201 2439 Size: 762 5149 SalI G'TCGA_C Cuts at: 1936 3253 1936 Size: 1317 4594 SapI GCTCTTCn'nnn_ Cuts at: 2348 2348 Size: 5911 SbfI CC_TGCA'GG Cuts at: 3263 3263 Size: 5911 ScaI AGT'ACT Cuts at: 4159 5871 4159 Size: 1712 4199 SfcI C'TryA_G Cuts at: 826 1045 2884 3259 4394 5075 5266 826 Size: 219 1839 375 1135 681 191 1471 Fragments arranged by size: 1839 1471 1135 681 375 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5911 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5911 SmaI CCC'GGG Cuts at: 1872 3238 1872 Size: 1366 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4015 4916 5178 820 Size: 197 926 2072 901 262 1553 Fragments arranged by size: 2072 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3702 493 Size: 3209 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5911 SphI G_CATG'C Cuts at: 1132 3269 1132 Size: 2137 3774 SspI AAT'ATT Cuts at: 5 52 3835 5 Size: 47 3783 2081 Fragments arranged by size: 3783 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5911 StyI C'CwwG_G Cuts at: 513 1065 2874 3350 513 Size: 552 1809 476 3074 Fragments arranged by size: 3074 1809 552 476 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2896 3938 3955 4108 4293 4738 325 Size: 1255 1316 1042 17 153 185 445 1498 Fragments arranged by size: 1498 1316 1255 1042 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2232 3031 4157 Size: 274 80 33 51 1443 255 799 1126 Cuts at: 4157 5869 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1126 799 274 255 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2711 2743 2914 2946 5871 5903 2711 Size: 32 171 32 2925 32 2719 Fragments arranged by size: 2925 2719 171 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5911 XbaI T'CTAG_A Cuts at: 3247 3247 Size: 5911 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2955 3032 2955 Size: 77 5834 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5911 XmaI C'CCGG_G Cuts at: 1870 3236 1870 Size: 1366 4545 XmnI GAAnn'nnTTC Cuts at: 2613 4040 2613 Size: 1427 4484 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3719 276 Size: 53 83 186 936 2185 2468 Fragments arranged by size: 2468 2185 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI