(Circular) (Six-base) MAPSORT of: pFN21AB7814.seq Check: 6716 from: 1 to: 6466 pFN21AB7814 6466 bp With 182 enzymes: SgfI * October 20, 2009 10:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3634 3634 Size: 6466 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3332 4276 278 Size: 53 83 186 936 1796 944 2468 Fragments arranged by size: 2468 1796 944 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2424 3009 3809 1937 Size: 487 585 800 4594 Fragments arranged by size: 4594 800 585 487 Acc65I G'GTAC_C Cuts at: 3787 3787 Size: 6466 AclI AA'CG_TT Cuts at: 1563 4593 4966 1563 Size: 3030 373 3063 Fragments arranged by size: 3063 3030 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3137 3280 4529 5913 6181 1632 Size: 1505 143 1249 1384 268 1917 Fragments arranged by size: 1917 1505 1384 1249 268 143 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6269 AflIII A'CryG_T Cuts at: 1203 2697 3318 5365 6342 1203 Size: 1494 621 2047 977 1327 Fragments arranged by size: 2047 1494 1327 977 621 AgeI A'CCGG_T Cuts at: 5297 5297 Size: 6466 AhdI GACnn_n'nnGTC Cuts at: 5195 5195 Size: 6466 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6466 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6466 AlwNI CAG_nnn'CTG Cuts at: 2543 4209 5781 2543 Size: 1666 1572 3228 Fragments arranged by size: 3228 1666 1572 ApaI G_GGCC'C Cuts at: 2737 2737 Size: 6466 ApaLI G'TGCA_C Cuts at: 2705 4523 5679 2705 Size: 1818 1156 3492 Fragments arranged by size: 3492 1818 1156 ApoI r'AATT_y Cuts at: 1493 3775 4001 4153 6262 1493 Size: 2282 226 152 2109 1697 Fragments arranged by size: 2282 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5020 160 Size: 872 3988 1606 Fragments arranged by size: 3988 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6466 AvaI C'yCGr_G Cuts at: 1870 1943 2619 3791 1870 Size: 73 676 1172 4545 Fragments arranged by size: 4545 1172 676 73 BamHI G'GATC_C Cuts at: 3796 4163 3796 Size: 367 6099 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2810 3464 3787 5242 618 Size: 325 217 627 1023 654 323 1455 1842 Fragments arranged by size: 1842 1455 1023 654 627 325 323 217 BanII G_rGCy'C Cuts at: 1391 2737 3785 1391 Size: 1346 1048 4072 Fragments arranged by size: 4072 1346 1048 BbeI G_GCGC'C Cuts at: 3468 3468 Size: 6466 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5495 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3552 3586 4657 4691 1903 Size: 34 1615 34 1071 34 3678 Fragments arranged by size: 3678 1615 1071 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4358 5568 4358 Size: 1210 5256 BclI T'GATC_A Cuts at: 1548 1548 Size: 6466 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2126 5077 136 Size: 107 122 71 631 605 454 2951 1525 Fragments arranged by size: 2951 1525 631 605 454 122 107 71 BglII A'GATC_T Cuts at: 2377 6461 2377 Size: 4084 2382 BlpI GC'TnA_GC Cuts at: 3883 3883 Size: 6466 Bme1580I G_kGCm'C Cuts at: 1790 2709 2737 4527 5683 1790 Size: 919 28 1790 1156 2573 Fragments arranged by size: 2573 1790 1156 919 28 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6466 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2781 4197 5155 Size: 416 53 83 399 24 1364 1416 958 Cuts at: 5155 442 Size: 1753 Fragments arranged by size: 1753 1416 1364 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3311 1056 Size: 2255 4211 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2324 5126 1239 Size: 1085 2802 2579 Fragments arranged by size: 2802 2579 1085 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2564 4591 5456 5754 2564 Size: 2027 865 298 3276 Fragments arranged by size: 3276 2027 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5129 882 Size: 617 3630 2219 Fragments arranged by size: 3630 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2677 4257 493 Size: 711 1473 1580 2702 Fragments arranged by size: 2702 1580 1473 711 BsaBI GATnn'nnATC Cuts at: 6460 6460 Size: 6466 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2118 2943 3285 3329 Size: 53 83 186 936 585 825 342 44 Cuts at: 3329 3465 4273 4655 5290 275 Size: 136 808 382 635 1451 Fragments arranged by size: 1451 936 825 808 635 585 382 342 186 136 83 53 44 BsaWI w'CCGG_w Cuts at: 2190 4899 5297 5571 5718 6129 2190 Size: 2709 398 274 147 411 2527 Fragments arranged by size: 2709 2527 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6241 Fragments arranged by size: 6241 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2069 3505 3684 3687 1186 Size: 883 1436 179 3 3965 Fragments arranged by size: 3965 1436 883 179 3 BseYI C'CCAG_C Cuts at: 2062 3398 3411 5669 6165 2062 Size: 1336 13 2258 496 2363 Fragments arranged by size: 2363 2258 1336 496 13 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2506 3249 3691 1163 Size: 573 770 743 442 3938 Fragments arranged by size: 3938 770 743 573 442 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2130 3916 4677 5705 1540 Size: 275 186 129 1786 761 1028 2301 Fragments arranged by size: 2301 1786 1028 761 275 186 129 BsiHKAI G_wGCw'C Cuts at: 2709 2995 4527 4612 5683 2709 Size: 286 1532 85 1071 3492 Fragments arranged by size: 3492 1532 1071 286 85 BsmI GAATG_Cn' Cuts at: 2611 3159 3977 4070 2611 Size: 548 818 93 5007 Fragments arranged by size: 5007 818 548 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3320 3320 Size: 6466 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2709 2737 2995 3785 4527 4612 5683 Size: 399 919 28 258 790 742 85 1071 Cuts at: 5683 1391 Size: 2174 Fragments arranged by size: 2174 1071 919 790 742 399 258 85 28 BspEI T'CCGG_A Cuts at: 2190 6129 2190 Size: 3939 2527 BspHI T'CATG_A Cuts at: 4355 6085 4355 Size: 1730 4736 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3634 3821 844 Size: 2790 187 3489 Fragments arranged by size: 3489 2790 187 BsrBI CCG'CTC Cuts at: 1394 1823 2139 3191 4353 1394 Size: 429 316 1052 1162 3507 Fragments arranged by size: 3507 1162 1052 429 316 BsrDI GCAATG_nn' Cuts at: 65 1248 4961 5135 65 Size: 1183 3713 174 1396 Fragments arranged by size: 3713 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2245 2490 5110 5297 1700 Size: 110 435 245 2620 187 2869 Fragments arranged by size: 2869 2620 435 245 187 110 BsrGI T'GTAC_A Cuts at: 96 2349 3213 96 Size: 2253 864 3349 Fragments arranged by size: 3349 2253 864 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6466 BssSI C'ACGA_G Cuts at: 2089 2474 4526 5538 2089 Size: 385 2052 1012 3017 Fragments arranged by size: 3017 2052 1012 385 BstAPI GCAn_nnn'nTGC Cuts at: 794 2493 794 Size: 1699 4767 BstBI TT'CG_AA Cuts at: 5270 5270 Size: 6466 BstEII G'GTnAC_C Cuts at: 1184 3096 3574 1184 Size: 1912 478 4076 Fragments arranged by size: 4076 1912 478 BstYI r'GATC_y Cuts at: 1972 2377 3240 3796 4163 4548 4565 6017 6103 Size: 405 863 556 367 385 17 1452 86 Cuts at: 6103 6115 6461 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 863 556 405 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2802 3468 3558 3672 513 Size: 552 1737 666 90 114 3307 Fragments arranged by size: 3307 1737 666 552 114 90 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3184 487 Size: 683 56 1958 3769 Fragments arranged by size: 3769 1958 683 56 BtsI GCAGTG_nn' Cuts at: 3651 3982 4776 4796 3651 Size: 331 794 20 5321 Fragments arranged by size: 5321 794 331 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6466 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6431 DraI TTT'AAA Cuts at: 3770 3770 Size: 6466 DraIII CAC_nnn'GTG Cuts at: 2215 2766 2215 Size: 551 5915 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5473 809 Size: 507 4157 1802 Fragments arranged by size: 4157 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2127 2671 2682 Size: 54 1425 50 275 18 297 544 11 Cuts at: 2682 3471 3913 4802 6431 8 Size: 789 442 889 1629 43 Fragments arranged by size: 1629 1425 889 789 544 442 297 275 54 50 43 18 11 EagI C'GGCC_G Cuts at: 1537 1812 2127 3913 1537 Size: 275 315 1786 4090 Fragments arranged by size: 4090 1786 315 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3574 3650 4396 1353 Size: 2221 76 746 3423 Fragments arranged by size: 3423 2221 746 76 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3143 3209 5053 5427 5573 387 Size: 1064 138 1554 66 1844 374 146 1280 Fragments arranged by size: 1844 1554 1280 1064 374 146 138 66 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2324 3137 3280 4529 5126 5913 6181 Size: 393 692 813 143 1249 597 787 268 Cuts at: 6181 1239 Size: 1524 Fragments arranged by size: 1524 1249 813 787 692 597 393 268 143 EcoO109I rG'GnC_Cy Cuts at: 3484 3484 Size: 6466 EcoRI G'AATT_C Cuts at: 3775 4153 3775 Size: 378 6088 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2283 3679 2283 Size: 1396 5070 FspI TGC'GCA Cuts at: 4972 4972 Size: 6466 HaeII r_GCGC'y Cuts at: 1678 2225 3004 3212 3468 3663 3717 5613 1678 Size: 547 779 208 256 195 54 1896 2531 Fragments arranged by size: 2531 1896 779 547 256 208 195 54 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3378 3410 5107 5139 5181 Size: 32 135 32 1806 32 1697 32 42 Cuts at: 5181 5213 1373 Size: 32 2626 Fragments arranged by size: 2626 1806 1697 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2662 3289 3316 3810 4056 669 Size: 988 281 724 627 27 494 246 3079 Fragments arranged by size: 3079 988 724 627 494 281 246 27 HindIII A'AGCT_T Cuts at: 748 748 Size: 6466 HpaI GTT'AAC Cuts at: 4056 4056 Size: 6466 KasI G'GCGC_C Cuts at: 3464 3464 Size: 6466 KpnI G_GTAC'C Cuts at: 3791 3791 Size: 6466 MfeI C'AATT_G Cuts at: 4065 4065 Size: 6466 MluI A'CGCG_T Cuts at: 6342 6342 Size: 6466 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2208 5580 5764 1305 Size: 903 3372 184 2007 Fragments arranged by size: 3372 2007 903 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3473 10 Size: 54 1425 343 1641 3003 Fragments arranged by size: 3003 1641 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2702 3081 3275 3443 4424 4783 4942 Size: 648 1536 379 194 168 981 359 159 Cuts at: 4942 518 Size: 2042 Fragments arranged by size: 2042 1536 981 648 379 359 194 168 159 MspA1I CmG'CkG Cuts at: 1606 2062 2070 3882 4559 5707 5952 1606 Size: 456 8 1812 677 1148 245 2120 Fragments arranged by size: 2120 1812 1148 677 456 245 8 NarI GG'CG_CC Cuts at: 3465 3465 Size: 6466 NcoI C'CATG_G Cuts at: 513 1065 3468 3558 513 Size: 552 2403 90 3421 Fragments arranged by size: 3421 2403 552 90 NdeI CA'TA_TG Cuts at: 387 387 Size: 6466 NheI G'CTAG_C Cuts at: 1052 3307 1052 Size: 2255 4211 NotI GC'GGCC_GC Cuts at: 3913 3913 Size: 6466 NspI r_CATG'y Cuts at: 1132 1229 2701 3824 5369 1132 Size: 97 1472 1123 1545 2229 Fragments arranged by size: 2229 1545 1472 1123 97 PasI CC'CwG_GG Cuts at: 1348 3250 1348 Size: 1902 4564 PciI A'CATG_T Cuts at: 2697 5365 2697 Size: 2668 3798 PflMI CCAn_nnn'nTGG Cuts at: 1108 2680 1108 Size: 1572 4894 PmeI GTTT'AAAC Cuts at: 3770 3770 Size: 6466 PmlI CAC'GTG Cuts at: 2677 2677 Size: 6466 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4535 4567 6048 6080 4535 Size: 32 1481 32 4921 Fragments arranged by size: 4921 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3484 3484 Size: 6466 PshAI GACnn'nnGTC Cuts at: 1613 2657 1613 Size: 1044 5422 PsiI TTA'TAA Cuts at: 4036 4036 Size: 6466 PspOMI G'GGCC_C Cuts at: 2733 2733 Size: 6466 PstI C_TGCA'G Cuts at: 830 3818 830 Size: 2988 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6466 SalI G'TCGA_C Cuts at: 1936 3808 1936 Size: 1872 4594 SanDI GG'GwC_CC Cuts at: 3484 3484 Size: 6466 SapI GCTCTTCn'nnn_ Cuts at: 3650 3650 Size: 6466 SbfI CC_TGCA'GG Cuts at: 3818 3818 Size: 6466 ScaI AGT'ACT Cuts at: 3406 4714 6426 3406 Size: 1308 1712 3446 Fragments arranged by size: 3446 1712 1308 SexAI A'CCwGG_T Cuts at: 3132 3132 Size: 6466 SfcI C'TryA_G Cuts at: 826 1045 3355 3592 3814 4949 5630 5821 826 Size: 219 2310 237 222 1135 681 191 1471 Fragments arranged by size: 2310 1471 1135 681 237 222 219 191 SfoI GGC'GCC Cuts at: 3466 3466 Size: 6466 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6466 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6466 SmaI CCC'GGG Cuts at: 1872 3793 1872 Size: 1921 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2579 2619 4570 5471 5733 820 Size: 197 926 636 40 1951 901 262 1553 Fragments arranged by size: 1951 1553 926 901 636 262 197 40 SnaBI TAC'GTA Cuts at: 493 4257 493 Size: 3764 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6466 SphI G_CATG'C Cuts at: 1132 3824 1132 Size: 2692 3774 SspI AAT'ATT Cuts at: 5 52 2026 4390 5 Size: 47 1974 2364 2081 Fragments arranged by size: 2364 2081 1974 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6466 StyI C'CwwG_G Cuts at: 513 1065 3102 3468 3558 3905 513 Size: 552 2037 366 90 347 3074 Fragments arranged by size: 3074 2037 552 366 347 90 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3562 4493 4510 4663 4848 5293 325 Size: 1255 1982 931 17 153 185 445 1498 Fragments arranged by size: 1982 1498 1255 931 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2349 3213 3404 Size: 274 80 33 51 1443 372 864 191 Cuts at: 3404 4712 6424 96 Size: 1308 1712 138 Fragments arranged by size: 1712 1443 1308 864 372 274 191 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2699 2731 6426 6458 2699 Size: 32 3695 32 2707 Fragments arranged by size: 3695 2707 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3347 1316 Size: 2031 4435 XbaI T'CTAG_A Cuts at: 3802 3802 Size: 6466 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3556 3556 Size: 6466 XhoI C'TCGA_G Cuts at: 1943 2619 1943 Size: 676 5790 XmaI C'CCGG_G Cuts at: 1870 3791 1870 Size: 1921 4545 XmnI GAAnn'nnTTC Cuts at: 4595 4595 Size: 6466 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3330 4274 276 Size: 53 83 186 936 1796 944 2468 Fragments arranged by size: 2468 1796 944 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PspXI PsrI PvuII RsrII SacI SacII SfiI SrfI SwaI