Restriction map for
pFN21ASDA2008
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# NO CUT ENZYMES ( 99 enzymes) #
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AccIII AclI AcsI AflII AhdI
Alw44I AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I AspEI
AspI BbsI BbvCI BcgI BciVI
BfrI BglII BlpI Bpu1102I BpuAI
BsaBI BseAI BsiEI BsiWI BsmI
Bsp1407I BspDI BspEI BspLU11I BsrDI
BsrGI BssHII Bst1107I BstBI BstZ17I
Bsu36I CelII ClaI CpoI DraI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoRI EcoRV EcoT22I HindIII
HpaI MamI MfeI MluI MroI
MseI MunI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PflFI PmeI PshAI PshBI Psp1406I
PvuI RsrII SbfI ScaI SexAI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tru9I Tsp509I Tth111I XbaI
XcmI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AatII 114 GACGTC GACGT^C
Acc65I 1035 GGTACC G^GTACC
AgeI 1154 ACCGGT A^CCGGT
Asp718I 1035 GGTACC G^GTACC
AvrII 930 CCTAGG C^CTAGG
BbeI 143 GGCGCC GGCGC^C
BbrPI 1777 CACGTG CAC^GTG
BclI 2 TGATCA T^GATCA
BfaI 931 CTAG C^TAG
BlnI 930 CCTAGG C^CTAGG
BsaAI 1777 CACGTG [CT]AC^GT[GA]
BsaI 57 GAGACC GGTCTC(1/5)
BstEII 48 GGTCACC G^GT[ACGT]ACC
BstPI 48 GGTCACC G^GT[ACGT]ACC
BstXI 1376 CCAGCAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 879 GACGGAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 48 GGTCACC G^GT[ACGT]ACC
FbaI 2 TGATCA T^GATCA
FseI 342 GGCCGGCC GGCCGG^CC
KasI 143 GGCGCC G^GCGCC
KpnI 1035 GGTACC GGTAC^C
KspI 201 CCGCGG CCGC^GG
MaeI 931 CTAG C^TAG
NarI 143 GGCGCC GG^CGCC
PaeR7I 2008 CTCGAG C^TCGAG
PinAI 1154 ACCGGT A^CCGGT
PmaCI 1777 CACGTG CAC^GTG
PmlI 1777 CACGTG CAC^GTG
PpuMI 911 GGGTCCT [GA]G^G[AT]CC[CT]
SacII 201 CCGCGG CCGC^GG
SalI 1789 GTCGAC G^TCGAC
SapI 2280 GCTCTTC GCTCTTC(1/4)
SfiI 2070 GGCCTGGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 143 GGCGCC GGC^GCC
TfiI 1163 GAATC G^A[AT]TC
XhoI 2008 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AccI 1789 GTCGAC GT^[AC][GT]AC
1849 GTAGAC
AflIII 896 ACGTGT A^C[GA][CT]GT
1778 ACGTGT
AviII 212 TGCGCA TGC^GCA
1571 TGCGCA
BalI 263 TGGCCA TGG^CCA
2247 TGGCCA
BamHI 359 GGATCC G^GATCC
1898 GGATCC
BsaWI 1154 ACCGGT [AT]^CCGG[AT]
1235 ACCGGA
BspHI 758 TCATGA T^CATGA
1277 TCATGA
BssSI 1207 CACGAG CACGAG(-5/-1)
1696 CACGAG
DraIII 476 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
576 CACCGAGTG
EarI 1585 CTCTTC CTCTTC(1/4)
2281 CTCTTC
FspI 212 TGCGCA TGC^GCA
1571 TGCGCA
HincII 10 GTCAAC GT[CT]^[GA]AC
1789 GTCGAC
HindII 10 GTCAAC GT[CT]^[GA]AC
1789 GTCGAC
Ksp632I 1585 CTCTTC CTCTTC(1/4)
2281 CTCTTC
MluNI 263 TGGCCA TGG^CCA
2247 TGGCCA
MscI 263 TGGCCA TGG^CCA
2247 TGGCCA
NcoI 984 CCATGG C^CATGG
1473 CCATGG
PflMI 714 CCACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
807 CCAGCGCCTGG
PvuII 1564 CAGCTG CAG^CTG
2286 CAGCTG
RcaI 758 TCATGA T^CATGA
1277 TCATGA
SacI 1333 GAGCTC GAGCT^C
1934 GAGCTC
SmlI 2008 CTCGAG C^T[CT][GA]AG
2176 CTCAAG
Van91I 714 CCACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
807 CCAGCGCCTGG
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# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BanI BanII BbvI BcnI
BglI BmyI BpmI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TseI Tsp45I TspRI TthHB8I XhoII