(Circular) (Six-base) MAPSORT of: pFN21ASDA2008.seq Check: 8979 from: 1 to: 7105 pFN21ASDA2008 7105 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3908 4139 4198 3908 Size: 231 59 6815 Fragments arranged by size: 6815 231 59 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2141 4915 278 Size: 53 83 186 936 605 2774 2468 Fragments arranged by size: 2774 2468 936 605 186 83 53 AccI GT'mk_AC Cuts at: 1937 3813 3873 4448 1937 Size: 1876 60 575 4594 Fragments arranged by size: 4594 1876 575 60 Acc65I G'GTAC_C Cuts at: 3058 4426 3058 Size: 1368 5737 AclI AA'CG_TT Cuts at: 1563 5232 5605 1563 Size: 3669 373 3063 Fragments arranged by size: 3669 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2960 3053 3676 4291 5168 6552 6820 1632 Size: 1328 93 623 615 877 1384 268 1917 Fragments arranged by size: 1917 1384 1328 877 623 615 268 93 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6908 AflIII A'CryG_T Cuts at: 1203 2919 3801 6004 6981 1203 Size: 1716 882 2203 977 1327 Fragments arranged by size: 2203 1716 1327 977 882 AgeI A'CCGG_T Cuts at: 3177 5936 3177 Size: 2759 4346 AhdI GACnn_n'nnGTC Cuts at: 5834 5834 Size: 7105 AleI CACnn'nnGTG Cuts at: 1166 2201 1166 Size: 1035 6070 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2327 3695 1184 Size: 1143 1368 4594 Fragments arranged by size: 4594 1368 1143 AlwNI CAG_nnn'CTG Cuts at: 2437 2836 2848 2881 3589 3592 3968 4848 6420 Size: 399 12 33 708 3 376 880 1572 Cuts at: 6420 2437 Size: 3122 Fragments arranged by size: 3122 1572 880 708 399 376 33 12 3 ApaI G_GGCC'C Cuts at: 2216 2952 3641 2216 Size: 736 689 5680 Fragments arranged by size: 5680 736 689 ApaLI G'TGCA_C Cuts at: 5162 6318 5162 Size: 1156 5949 ApoI r'AATT_y Cuts at: 1493 4414 4640 4792 6901 1493 Size: 2921 226 152 2109 1697 Fragments arranged by size: 2921 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5659 160 Size: 872 4627 1606 Fragments arranged by size: 4627 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7105 AvaI C'yCGr_G Cuts at: 1870 1943 2216 2931 3530 4031 4430 1870 Size: 73 273 715 599 501 399 4545 Fragments arranged by size: 4545 715 599 501 399 273 73 AvrII C'CTAG_G Cuts at: 2953 2953 Size: 7105 BamHI G'GATC_C Cuts at: 2382 3921 4435 4802 2382 Size: 1539 514 367 4685 Fragments arranged by size: 4685 1539 514 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2166 2497 2597 2686 3058 Size: 325 217 627 379 331 100 89 372 Cuts at: 3058 3180 3500 3535 4210 4426 5881 618 Size: 122 320 35 675 216 1455 1842 Fragments arranged by size: 1842 1455 675 627 379 372 331 325 320 217 216 122 100 89 35 BanII G_rGCy'C Cuts at: 1391 2216 2952 3360 3641 3961 4001 4042 4161 Size: 825 736 408 281 320 40 41 119 Cuts at: 4161 4424 1391 Size: 263 4072 Fragments arranged by size: 4072 825 736 408 320 281 263 119 41 40 BbeI G_GCGC'C Cuts at: 2170 2170 Size: 7105 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6134 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5296 5330 1903 Size: 34 3359 34 3678 Fragments arranged by size: 3678 3359 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4997 6207 4997 Size: 1210 5895 BclI T'GATC_A Cuts at: 1548 2025 1548 Size: 477 6628 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2684 2816 3851 Size: 107 122 71 631 605 1012 132 1035 Cuts at: 3851 4091 4100 5716 136 Size: 240 9 1616 1525 Fragments arranged by size: 1616 1525 1035 1012 631 605 240 132 122 107 71 9 BglII A'GATC_T Cuts at: 7100 7100 Size: 7105 BlpI GC'TnA_GC Cuts at: 4522 4522 Size: 7105 Bme1580I G_kGCm'C Cuts at: 1790 2216 2554 2689 2952 3641 4215 5166 6322 Size: 426 338 135 263 689 574 951 1156 Cuts at: 6322 1790 Size: 2573 Fragments arranged by size: 2573 1156 951 689 574 426 338 263 135 BmgBI CAC'GTC Cuts at: 1318 2920 1318 Size: 1602 5503 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2496 2806 3277 Size: 416 53 83 399 24 1079 310 471 Cuts at: 3277 4836 5794 442 Size: 1559 958 1753 Fragments arranged by size: 1753 1559 1079 958 471 416 399 310 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7105 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3775 3775 Size: 7105 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2276 2315 2680 3431 3671 3730 3935 3945 Size: 1037 39 365 751 240 59 205 10 Cuts at: 3945 5765 1239 Size: 1820 2579 Fragments arranged by size: 2579 1820 1037 751 365 240 205 59 39 10 Bpu10I CC'TnA_GC Cuts at: 2527 2527 Size: 7105 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4184 5230 6095 6393 4184 Size: 1046 865 298 4896 Fragments arranged by size: 4896 1046 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2074 5768 882 Size: 617 575 3694 2219 Fragments arranged by size: 3694 2219 617 575 BsaAI yAC'GTr Cuts at: 493 1204 3802 4402 4896 493 Size: 711 2598 600 494 2702 Fragments arranged by size: 2702 2598 711 600 494 BsaBI GATnn'nnATC Cuts at: 7099 7099 Size: 7105 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2138 2167 2229 4191 Size: 53 83 186 936 605 29 62 1962 Cuts at: 4191 4912 5294 5929 275 Size: 721 382 635 1451 Fragments arranged by size: 1962 1451 936 721 635 605 382 186 83 62 53 29 BsaWI w'CCGG_w Cuts at: 3177 3258 5538 5936 6210 6357 6768 3177 Size: 81 2280 398 274 147 411 3514 Fragments arranged by size: 3514 2280 411 398 274 147 81 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6880 Fragments arranged by size: 6880 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2921 3248 3368 3845 1186 Size: 1735 327 120 477 4446 Fragments arranged by size: 4446 1735 477 327 120 BseYI C'CCAG_C Cuts at: 2829 2848 3762 4263 6308 6804 2829 Size: 19 914 501 2045 496 3130 Fragments arranged by size: 3130 2045 914 501 496 19 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2297 2318 2441 2973 2987 3233 4105 Size: 573 561 21 123 532 14 246 872 Cuts at: 4105 1163 Size: 4163 Fragments arranged by size: 4163 872 573 561 532 246 123 21 14 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4555 5316 6344 1540 Size: 275 186 2554 761 1028 2301 Fragments arranged by size: 2554 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2609 3360 3961 5166 5251 6322 2609 Size: 751 601 1205 85 1071 3392 Fragments arranged by size: 3392 1205 1071 751 601 85 BsmI GAATG_Cn' Cuts at: 4616 4709 4616 Size: 93 7012 BsmBI CGTCTCn'nnnn_ Cuts at: 2145 2410 3359 2145 Size: 265 949 5891 Fragments arranged by size: 5891 949 265 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2216 2554 2609 2689 2952 3360 3641 Size: 399 426 338 55 80 263 408 281 Cuts at: 3641 3961 4001 4042 4161 4215 4424 5166 5251 Size: 320 40 41 119 54 209 742 85 Cuts at: 5251 6322 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 426 408 399 338 320 281 263 209 119 85 80 55 54 41 40 BspEI T'CCGG_A Cuts at: 6768 6768 Size: 7105 BspHI T'CATG_A Cuts at: 2781 3300 4994 6724 2781 Size: 519 1694 1730 3162 Fragments arranged by size: 3162 1730 1694 519 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2259 2340 3226 3908 4139 4198 4293 4460 Size: 1415 81 886 682 231 59 95 167 Cuts at: 4460 844 Size: 3489 Fragments arranged by size: 3489 1415 886 682 231 167 95 81 59 BsrBI CCG'CTC Cuts at: 1394 1823 2380 3085 3919 4992 1394 Size: 429 557 705 834 1073 3507 Fragments arranged by size: 3507 1073 834 705 557 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5600 5774 65 Size: 1183 4352 174 1396 Fragments arranged by size: 4352 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2321 2366 2478 3177 4316 4338 5749 Size: 110 511 45 112 699 1139 22 1411 Cuts at: 5749 5936 1700 Size: 187 2869 Fragments arranged by size: 2869 1411 1139 699 511 187 112 110 45 22 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7105 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7105 BssSI C'ACGA_G Cuts at: 3230 3719 5165 6177 3230 Size: 489 1446 1012 4158 Fragments arranged by size: 4158 1446 1012 489 BstAPI GCAn_nnn'nTGC Cuts at: 794 2604 3592 4087 794 Size: 1810 988 495 3812 Fragments arranged by size: 3812 1810 988 495 BstBI TT'CG_AA Cuts at: 2007 5909 2007 Size: 3902 3203 BstEII G'GTnAC_C Cuts at: 1184 2071 1184 Size: 887 6218 BstXI CCAn_nnnn'nTGG Cuts at: 3406 3406 Size: 7105 BstYI r'GATC_y Cuts at: 1972 2382 2433 2877 3492 3921 4435 4802 5187 Size: 410 51 444 615 429 514 367 385 Cuts at: 5187 5204 6656 6742 6754 7100 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 615 514 444 429 410 385 367 346 86 51 17 12 BtgI C'CryG_G Cuts at: 513 1065 2224 3007 3127 3411 3496 3556 4141 Size: 552 1159 783 120 284 85 60 585 Cuts at: 4141 513 Size: 3477 Fragments arranged by size: 3477 1159 783 585 552 284 120 85 60 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2398 3643 487 Size: 683 56 1172 1245 3949 Fragments arranged by size: 3949 1245 1172 683 56 BtsI GCAGTG_nn' Cuts at: 2311 2975 4621 5415 5435 2311 Size: 664 1646 794 20 3981 Fragments arranged by size: 3981 1646 794 664 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7105 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7070 DraI TTT'AAA Cuts at: 4409 4409 Size: 7105 DraIII CAC_nnn'GTG Cuts at: 2504 2604 2504 Size: 100 7005 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2908 6112 809 Size: 507 1592 3204 1802 Fragments arranged by size: 3204 1802 1592 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2286 3408 4101 Size: 54 1425 50 275 18 456 1122 693 Cuts at: 4101 4270 4552 5441 7070 8 Size: 169 282 889 1629 43 Fragments arranged by size: 1629 1425 1122 889 693 456 282 275 169 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4552 1537 Size: 275 2740 4090 Fragments arranged by size: 4090 2740 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3614 4310 5035 1353 Size: 2261 696 725 3423 Fragments arranged by size: 3423 2261 725 696 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2990 5692 6066 6212 387 Size: 1064 138 1401 2702 374 146 1280 Fragments arranged by size: 2702 1401 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3358 3959 3358 Size: 601 6504 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2276 2315 2680 2960 3053 3431 3671 Size: 393 644 39 365 280 93 378 240 Cuts at: 3671 3676 3730 3935 3945 4291 5168 5765 6552 Size: 5 54 205 10 346 877 597 787 Cuts at: 6552 6820 1239 Size: 268 1524 Fragments arranged by size: 1524 877 787 644 597 393 378 365 346 280 268 240 205 93 54 39 10 5 EcoNI CCTnn'n_nnAGG Cuts at: 2627 2903 3977 4289 2627 Size: 276 1074 312 5443 Fragments arranged by size: 5443 1074 312 276 EcoO109I rG'GnC_Cy Cuts at: 2212 2213 2935 2949 3637 3638 4065 4116 2212 Size: 1 722 14 688 1 427 51 5201 Fragments arranged by size: 5201 722 688 427 51 14 1 1 EcoRI G'AATT_C Cuts at: 4414 4792 4414 Size: 378 6727 FseI GG_CCGG'CC Cuts at: 2370 2370 Size: 7105 FspI TGC'GCA Cuts at: 2237 3596 5611 2237 Size: 1359 2015 3731 Fragments arranged by size: 3731 2015 1359 HaeII r_GCGC'y Cuts at: 1678 2170 2822 2836 6252 1678 Size: 492 652 14 3416 2531 Fragments arranged by size: 3416 2531 652 492 14 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2055 2087 2125 2157 5746 Size: 32 135 32 483 32 38 32 3589 Cuts at: 5746 5778 5820 5852 1373 Size: 32 42 32 2626 Fragments arranged by size: 3589 2626 483 135 42 38 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2035 3814 4449 4695 669 Size: 988 281 97 1779 635 246 3079 Fragments arranged by size: 3079 1779 988 635 281 246 97 HindIII A'AGCT_T Cuts at: 748 748 Size: 7105 HpaI GTT'AAC Cuts at: 4695 4695 Size: 7105 KasI G'GCGC_C Cuts at: 2166 2166 Size: 7105 KpnI G_GTAC'C Cuts at: 3062 4430 3062 Size: 1368 5737 MfeI C'AATT_G Cuts at: 4704 4704 Size: 7105 MluI A'CGCG_T Cuts at: 6981 6981 Size: 7105 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2433 4212 4347 6219 6403 1305 Size: 1128 1779 135 1872 184 2007 Fragments arranged by size: 2007 1872 1779 1128 184 135 MscI TGG'CCA Cuts at: 10 64 1489 1832 2288 4272 10 Size: 54 1425 343 456 1984 2843 Fragments arranged by size: 2843 1984 1425 456 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2201 2417 3305 3410 5063 5422 5581 Size: 648 1035 216 888 105 1653 359 159 Cuts at: 5581 518 Size: 2042 Fragments arranged by size: 2042 1653 1035 888 648 359 216 159 105 MspA1I CmG'CkG Cuts at: 1606 2226 2556 2653 2760 3523 3589 4311 4521 Size: 620 330 97 107 763 66 722 210 Cuts at: 4521 5198 6346 6591 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 763 722 677 620 330 245 210 107 97 66 NaeI GCC'GGC Cuts at: 2323 2368 2480 4318 4340 2323 Size: 45 112 1838 22 5088 Fragments arranged by size: 5088 1838 112 45 22 NarI GG'CG_CC Cuts at: 2167 2167 Size: 7105 NcoI C'CATG_G Cuts at: 513 1065 3007 3496 513 Size: 552 1942 489 4122 Fragments arranged by size: 4122 1942 552 489 NdeI CA'TA_TG Cuts at: 387 387 Size: 7105 NgoMIV G'CCGG_C Cuts at: 2321 2366 2478 4316 4338 2321 Size: 45 112 1838 22 5088 Fragments arranged by size: 5088 1838 112 45 22 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7105 NotI GC'GGCC_GC Cuts at: 4552 4552 Size: 7105 NspI r_CATG'y Cuts at: 1132 1229 4463 6008 1132 Size: 97 3234 1545 2229 Fragments arranged by size: 3234 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2360 1348 Size: 1012 6093 PciI A'CATG_T Cuts at: 6004 6004 Size: 7105 PflMI CCAn_nnn'nTGG Cuts at: 1108 2743 2836 1108 Size: 1635 93 5377 Fragments arranged by size: 5377 1635 93 PmeI GTTT'AAAC Cuts at: 4409 4409 Size: 7105 PmlI CAC'GTG Cuts at: 3802 3802 Size: 7105 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5174 5206 6687 6719 5174 Size: 32 1481 32 5560 Fragments arranged by size: 5560 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2935 2935 Size: 7105 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7105 PsiI TTA'TAA Cuts at: 4675 4675 Size: 7105 PspOMI G'GGCC_C Cuts at: 2212 2948 3637 2212 Size: 736 689 5680 Fragments arranged by size: 5680 736 689 PspXI vC'TCGA_Gb Cuts at: 4031 4031 Size: 7105 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4232 4241 4264 4273 4232 Size: 9 23 9 7064 Fragments arranged by size: 7064 23 9 9 PstI C_TGCA'G Cuts at: 830 3045 3138 3684 3956 4209 4290 4457 830 Size: 2215 93 546 272 253 81 167 3478 Fragments arranged by size: 3478 2215 546 272 253 167 93 81 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7105 PvuII CAG'CTG Cuts at: 3589 4311 3589 Size: 722 6383 SacI G_AGCT'C Cuts at: 3360 3961 3360 Size: 601 6504 SacII CC_GC'GG Cuts at: 2227 2227 Size: 7105 SalI G'TCGA_C Cuts at: 1936 3812 4447 1936 Size: 1876 635 4594 Fragments arranged by size: 4594 1876 635 SapI GCTCTTCn'nnn_ Cuts at: 4310 4310 Size: 7105 SbfI CC_TGCA'GG Cuts at: 4457 4457 Size: 7105 ScaI AGT'ACT Cuts at: 5353 7065 5353 Size: 1712 5393 SfcI C'TryA_G Cuts at: 826 1045 3041 3134 3680 3952 4205 4286 4453 Size: 219 1996 93 546 272 253 81 167 Cuts at: 4453 5588 6269 6460 826 Size: 1135 681 191 1471 Fragments arranged by size: 1996 1471 1135 681 546 272 253 219 191 167 93 81 SfiI GGCCn_nnn'nGGCC Cuts at: 4100 4100 Size: 7105 SfoI GGC'GCC Cuts at: 2168 2168 Size: 7105 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7105 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7105 SmaI CCC'GGG Cuts at: 1872 4432 1872 Size: 2560 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4031 4199 5209 6110 6372 820 Size: 197 926 2088 168 1010 901 262 1553 Fragments arranged by size: 2088 1553 1010 926 901 262 197 168 SnaBI TAC'GTA Cuts at: 493 4402 4896 493 Size: 3909 494 2702 Fragments arranged by size: 3909 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7105 SphI G_CATG'C Cuts at: 1132 4463 1132 Size: 3331 3774 SspI AAT'ATT Cuts at: 5 52 5029 5 Size: 47 4977 2081 Fragments arranged by size: 4977 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7105 StyI C'CwwG_G Cuts at: 513 1065 2953 3007 3496 4544 513 Size: 552 1888 54 489 1048 3074 Fragments arranged by size: 3074 1888 1048 552 489 54 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2515 2704 2873 4040 5132 5149 5302 Size: 1255 935 189 169 1167 1092 17 153 Cuts at: 5302 5487 5932 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1167 1092 935 445 189 185 169 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3507 3894 5351 Size: 274 80 33 51 1443 1530 387 1457 Cuts at: 5351 7063 96 Size: 1712 138 Fragments arranged by size: 1712 1530 1457 1443 387 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2982 3014 7065 7097 2982 Size: 32 4051 32 2990 Fragments arranged by size: 4051 2990 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7105 XbaI T'CTAG_A Cuts at: 4441 4441 Size: 7105 XhoI C'TCGA_G Cuts at: 1943 4031 1943 Size: 2088 5017 XmaI C'CCGG_G Cuts at: 1870 4430 1870 Size: 2560 4545 XmnI GAAnn'nnTTC Cuts at: 5234 5234 Size: 7105 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2139 4913 276 Size: 53 83 186 936 605 2774 2468 Fragments arranged by size: 2774 2468 936 605 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BbvCI BfrBI BsiWI BstZ17I Bsu36I EcoRV FalI FspAI NruI NsiI PacI PfoI RsrII SanDI SexAI SrfI SwaI XcmI