Restriction map for
pFN21ASDA2008
(Linear) (Six-base) MAPSORT of: KIAA2008.seq Check: 5028 from: 1 to: 2376
KIAA2008.orf
With 182 enzymes: SgfI *
February 28, 2007 11:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1885 2116 2175 2376
Size: 1885 231 59 201
Fragments arranged by size:
1885 231 201 59
AatII G_ACGT'C
Cuts at: 0 118 2376
Size: 118 2258
AccI GT'mk_AC
Cuts at: 0 1790 1850 2376
Size: 1790 60 526
Fragments arranged by size:
1790 526 60
Acc65I G'GTAC_C
Cuts at: 0 1035 2376
Size: 1035 1341
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 937 1030 1653 2268 2376
Size: 937 93 623 615 108
Fragments arranged by size:
937 623 615 108 93
AflIII A'CryG_T
Cuts at: 0 896 1778 2376
Size: 896 882 598
Fragments arranged by size:
896 882 598
AgeI A'CCGG_T
Cuts at: 0 1154 2376
Size: 1154 1222
AleI CACnn'nnGTG
Cuts at: 0 178 2376
Size: 178 2198
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 304 1672 2376
Size: 304 1368 704
Fragments arranged by size:
1368 704 304
AlwNI CAG_nnn'CTG
Cuts at: 0 414 813 825 858 1566 1569 1945 2376
Size: 414 399 12 33 708 3 376 431
Fragments arranged by size:
708 431 414 399 376 33 12 3
ApaI G_GGCC'C
Cuts at: 0 193 929 1618 2376
Size: 193 736 689 758
Fragments arranged by size:
758 736 689 193
AvaI C'yCGr_G
Cuts at: 0 193 908 1507 2008 2376
Size: 193 715 599 501 368
Fragments arranged by size:
715 599 501 368 193
AvrII C'CTAG_G
Cuts at: 0 930 2376
Size: 930 1446
BamHI G'GATC_C
Cuts at: 0 359 1898 2376
Size: 359 1539 478
Fragments arranged by size:
1539 478 359
BanI G'GyrC_C
Cuts at: 0 143 474 574 663 1035 1157 1477 1512
Size: 143 331 100 89 372 122 320 35
Cuts at: 1512 2187 2376
Size: 675 189
Fragments arranged by size:
675 372 331 320 189 143 122 100
89 35
BanII G_rGCy'C
Cuts at: 0 193 929 1337 1618 1938 1978 2019 2138
Size: 193 736 408 281 320 40 41 119
Cuts at: 2138 2376
Size: 238
Fragments arranged by size:
736 408 320 281 238 193 119 41
40
BbeI G_GCGC'C
Cuts at: 0 147 2376
Size: 147 2229
BclI T'GATC_A
Cuts at: 0 2 2376
Size: 2 2374
BglI GCCn_nnn'nGGC
Cuts at: 0 661 793 1828 2068 2077 2376
Size: 661 132 1035 240 9 299
Fragments arranged by size:
1035 661 299 240 132 9
Bme1580I G_kGCm'C
Cuts at: 0 193 531 666 929 1618 2192 2376
Size: 193 338 135 263 689 574 184
Fragments arranged by size:
689 574 338 263 193 184 135
BmgBI CAC'GTC
Cuts at: 0 897 2376
Size: 897 1479
BmrI ACTGGGnnnn_n'
Cuts at: 0 473 783 1254 2376
Size: 473 310 471 1122
Fragments arranged by size:
1122 473 471 310
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1752 2376
Size: 1752 624
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 292 657 1408 1648 1707 1912 1922
Size: 253 39 365 751 240 59 205 10
Cuts at: 1922 2376
Size: 454
Fragments arranged by size:
751 454 365 253 240 205 59 39
10
Bpu10I CC'TnA_GC
Cuts at: 0 504 2376
Size: 504 1872
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2161 2376
Size: 2161 215
BsaI GGTCTCn'nnnn_
Cuts at: 0 51 2376
Size: 51 2325
BsaAI yAC'GTr
Cuts at: 0 1779 2376
Size: 1779 597
BsaHI Gr'CG_yC
Cuts at: 0 115 144 206 2168 2376
Size: 115 29 62 1962 208
Fragments arranged by size:
1962 208 115 62 29
BsaWI w'CCGG_w
Cuts at: 0 1154 1235 2376
Size: 1154 81 1141
Fragments arranged by size:
1154 1141 81
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 898 1225 1345 1822 2376
Size: 898 327 120 477 554
Fragments arranged by size:
898 554 477 327 120
BseYI C'CCAG_C
Cuts at: 0 806 825 1739 2240 2376
Size: 806 19 914 501 136
Fragments arranged by size:
914 806 501 136 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 295 418 950 964 1210 2082 2376
Size: 274 21 123 532 14 246 872 294
Fragments arranged by size:
872 532 294 274 246 123 21 14
BsiHKAI G_wGCw'C
Cuts at: 0 586 1337 1938 2376
Size: 586 751 601 438
Fragments arranged by size:
751 601 586 438
BsmBI CGTCTCn'nnnn_
Cuts at: 0 122 387 1336 2376
Size: 122 265 949 1040
Fragments arranged by size:
1040 949 265 122
Bsp1286I G_dGCh'C
Cuts at: 0 193 531 586 666 929 1337 1618 1938
Size: 193 338 55 80 263 408 281 320
Cuts at: 1938 1978 2019 2138 2192 2376
Size: 40 41 119 54 184
Fragments arranged by size:
408 338 320 281 263 193 184 119
80 55 54 41 40
BspHI T'CATG_A
Cuts at: 0 758 1277 2376
Size: 758 519 1099
Fragments arranged by size:
1099 758 519
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 236 317 1203 1885 2116 2175 2270 2376
Size: 236 81 886 682 231 59 95 106
Fragments arranged by size:
886 682 236 231 106 95 81 59
BsrBI CCG'CTC
Cuts at: 0 357 1062 1896 2376
Size: 357 705 834 480
Fragments arranged by size:
834 705 480 357
BsrFI r'CCGG_y
Cuts at: 0 298 343 455 1154 2293 2315 2376
Size: 298 45 112 699 1139 22 61
Fragments arranged by size:
1139 699 298 112 61 45 22
BssSI C'ACGA_G
Cuts at: 0 1207 1696 2376
Size: 1207 489 680
Fragments arranged by size:
1207 680 489
BstAPI GCAn_nnn'nTGC
Cuts at: 0 581 1569 2064 2376
Size: 581 988 495 312
Fragments arranged by size:
988 581 495 312
BstEII G'GTnAC_C
Cuts at: 0 48 2376
Size: 48 2328
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1383 2376
Size: 1383 993
BstYI r'GATC_y
Cuts at: 0 359 410 854 1469 1898 2376
Size: 359 51 444 615 429 478
Fragments arranged by size:
615 478 444 429 359 51
BtgI C'CryG_G
Cuts at: 0 201 984 1104 1388 1473 1533 2118 2376
Size: 201 783 120 284 85 60 585 258
Fragments arranged by size:
783 585 284 258 201 120 85 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 375 1620 2376
Size: 375 1245 756
Fragments arranged by size:
1245 756 375
BtsI GCAGTG_nn'
Cuts at: 0 288 952 2376
Size: 288 664 1424
Fragments arranged by size:
1424 664 288
DraIII CAC_nnn'GTG
Cuts at: 0 481 581 2376
Size: 481 100 1795
Fragments arranged by size:
1795 481 100
DrdI GACnn_nn'nnGTC
Cuts at: 0 885 2376
Size: 885 1491
EaeI y'GGCC_r
Cuts at: 0 263 1385 2078 2247 2376
Size: 263 1122 693 169 129
Fragments arranged by size:
1122 693 263 169 129
EarI CTCTTCn'nnn_
Cuts at: 0 1591 2287 2376
Size: 1591 696 89
Fragments arranged by size:
1591 696 89
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 967 2376
Size: 967 1409
EcoICRI GAG'CTC
Cuts at: 0 1335 1936 2376
Size: 1335 601 440
Fragments arranged by size:
1335 601 440
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 292 657 937 1030 1408 1648 1653
Size: 253 39 365 280 93 378 240 5
Cuts at: 1653 1707 1912 1922 2268 2376
Size: 54 205 10 346 108
Fragments arranged by size:
378 365 346 280 253 240 205 108
93 54 39 10 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 604 880 1954 2266 2376
Size: 604 276 1074 312 110
Fragments arranged by size:
1074 604 312 276 110
EcoO109I rG'GnC_Cy
Cuts at: 0 189 190 912 926 1614 1615 2042 2093
Size: 189 1 722 14 688 1 427 51
Cuts at: 2093 2376
Size: 283
Fragments arranged by size:
722 688 427 283 189 51 14 1
1
FseI GG_CCGG'CC
Cuts at: 0 347 2376
Size: 347 2029
FspI TGC'GCA
Cuts at: 0 214 1573 2376
Size: 214 1359 803
Fragments arranged by size:
1359 803 214
HaeII r_GCGC'y
Cuts at: 0 147 799 813 2376
Size: 147 652 14 1563
Fragments arranged by size:
1563 652 147 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 32 64 102 134 2376
Size: 32 32 38 32 2242
Fragments arranged by size:
2242 38 32 32 32
HincII GTy'rAC
Cuts at: 0 12 1791 2376
Size: 12 1779 585
Fragments arranged by size:
1779 585 12
KasI G'GCGC_C
Cuts at: 0 143 2376
Size: 143 2233
KpnI G_GTAC'C
Cuts at: 0 1039 2376
Size: 1039 1337
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 410 2189 2324 2376
Size: 410 1779 135 52
Fragments arranged by size:
1779 410 135 52
MscI TGG'CCA
Cuts at: 0 265 2249 2376
Size: 265 1984 127
Fragments arranged by size:
1984 265 127
MslI CAynn'nnrTG
Cuts at: 0 178 394 1282 1387 2376
Size: 178 216 888 105 989
Fragments arranged by size:
989 888 216 178 105
MspA1I CmG'CkG
Cuts at: 0 203 533 630 737 1500 1566 2288 2376
Size: 203 330 97 107 763 66 722 88
Fragments arranged by size:
763 722 330 203 107 97 88 66
NaeI GCC'GGC
Cuts at: 0 300 345 457 2295 2317 2376
Size: 300 45 112 1838 22 59
Fragments arranged by size:
1838 300 112 59 45 22
NarI GG'CG_CC
Cuts at: 0 144 2376
Size: 144 2232
NcoI C'CATG_G
Cuts at: 0 984 1473 2376
Size: 984 489 903
Fragments arranged by size:
984 903 489
NgoMIV G'CCGG_C
Cuts at: 0 298 343 455 2293 2315 2376
Size: 298 45 112 1838 22 61
Fragments arranged by size:
1838 298 112 61 45 22
PasI CC'CwG_GG
Cuts at: 0 337 2376
Size: 337 2039
PflMI CCAn_nnn'nTGG
Cuts at: 0 720 813 2376
Size: 720 93 1563
Fragments arranged by size:
1563 720 93
PmlI CAC'GTG
Cuts at: 0 1779 2376
Size: 1779 597
PpuMI rG'GwC_Cy
Cuts at: 0 912 2376
Size: 912 1464
PspOMI G'GGCC_C
Cuts at: 0 189 925 1614 2376
Size: 189 736 689 762
Fragments arranged by size:
762 736 689 189
PspXI vC'TCGA_Gb
Cuts at: 0 2008 2376
Size: 2008 368
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2209 2218 2241 2250 2376
Size: 2209 9 23 9 126
Fragments arranged by size:
2209 126 23 9 9
PstI C_TGCA'G
Cuts at: 0 1022 1115 1661 1933 2186 2267 2376
Size: 1022 93 546 272 253 81 109
Fragments arranged by size:
1022 546 272 253 109 93 81
PvuII CAG'CTG
Cuts at: 0 1566 2288 2376
Size: 1566 722 88
Fragments arranged by size:
1566 722 88
SacI G_AGCT'C
Cuts at: 0 1337 1938 2376
Size: 1337 601 438
Fragments arranged by size:
1337 601 438
SacII CC_GC'GG
Cuts at: 0 204 2376
Size: 204 2172
SalI G'TCGA_C
Cuts at: 0 1789 2376
Size: 1789 587
SapI GCTCTTCn'nnn_
Cuts at: 0 2287 2376
Size: 2287 89
SfcI C'TryA_G
Cuts at: 0 1018 1111 1657 1929 2182 2263 2376
Size: 1018 93 546 272 253 81 113
Fragments arranged by size:
1018 546 272 253 113 93 81
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2077 2376
Size: 2077 299
SfoI GGC'GCC
Cuts at: 0 145 2376
Size: 145 2231
SmlI C'TyrA_G
Cuts at: 0 2008 2176 2376
Size: 2008 168 200
Fragments arranged by size:
2008 200 168
StyI C'CwwG_G
Cuts at: 0 930 984 1473 2376
Size: 930 54 489 903
Fragments arranged by size:
930 903 489 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 492 681 850 2017 2376
Size: 492 189 169 1167 359
Fragments arranged by size:
1167 492 359 189 169
TatI w'GTAC_w
Cuts at: 0 1484 1871 2376
Size: 1484 387 505
Fragments arranged by size:
1484 505 387
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 959 991 2376
Size: 959 32 1385
Fragments arranged by size:
1385 959 32
XhoI C'TCGA_G
Cuts at: 0 2008 2376
Size: 2008 368
ZraI GAC'GTC
Cuts at: 0 116 2376
Size: 116 2260
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AflIII AgeI AleI
AlfI AlwNI ApaI AvaI AvrII BamHI BanI BanII
BbeI BclI BglI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BseRI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI
BssSI BstAPI BstEII BstXI BstYI BtgI BtgZI BtsI
DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FseI FspI HaeII Hin4I HincII KasI KpnI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
PasI PflMI PmlI PpuMI PspOMI PspXI PsrI PstI
PvuII SacI SacII SalI SapI SfcI SfiI SfoI
SmlI StyI TaqII TatI TstI XhoI ZraI
Enzymes that do not cut:
AclI AfeI AflII AhdI AloI ApaLI ApoI AscI
AseI AsiSI BaeI BbsI BbvCI BcgI BciVI BfrBI
BglII BlpI BmtI BsaBI BsaXI BsiEI BsiWI BsmI
BspEI BsrDI BsrGI BssHII BstBI BstZ17I Bsu36I ClaI
CspCI DraI EagI EcoRI EcoRV FalI FspAI HindIII
HpaI MfeI MluI NdeI NheI NotI NruI NsiI
NspI PacI PciI PfoI PmeI PpiI PshAI PsiI
PvuI RsrII SanDI SbfI ScaI SexAI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
Tth111I XbaI XcmI XmaI XmnI