Restriction map for
pFN21ASDA1950
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# NO CUT ENZYMES ( 121 enzymes) #
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AatII AccIII AclI AflII AflIII
AgeI AhdI Alw44I AlwI Aor51HI
ApaLI AscI AseI AsnI Asp700I
AspEI AspI AvrII BamHI BbeI
BbsI BbvCI BciVI BclI BfaI
BfrI BglII BlnI BpuAI BsaBI
BseAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstYI BstZ17I
ClaI CpoI DraI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRV
EcoT14I EcoT22I FbaI FseI HincII
HindII HindIII HpaI KasI KspI
MaeI MamI MfeI MflI MluI
MroI MseI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
ScaI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI SspBI
SspI StyI SwaI TfiI Tru9I
Tth111I XbaI XcmI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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Acc65I 941 GGTACC G^GTACC
AccI 11 GTAGAC GT^[AC][GT]AC
AcsI 637 GAATTC [GA]^AATT[CT]
ApoI 637 GAATTC [GA]^AATT[CT]
Asp718I 941 GGTACC G^GTACC
AspHI 784 GAGCTC G[AT]GC[AT]^C
AviII 1388 TGCGCA TGC^GCA
BalI 1232 TGGCCA TGG^CCA
BbrPI 774 CACGTG CAC^GTG
BcgI 182 GCACGGGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1168 GCCTCAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 774 CACGTG [CT]AC^GT[GA]
BsaI 63 GGTCTC GGTCTC(1/5)
BsaWI 257 ACCGGA [AT]^CCGG[AT]
BsiEI 1311 CGACCG CG[GA][CT]^CG
BsiHKAI 784 GAGCTC G[AT]GC[AT]^C
BspMI 1284 GCAGGT ACCTGC(4/8)
BsrBI 118 GAGCGG CCGCTC(-3/-3)
BsrFI 318 GCCGGT [GA]^CCGG[CT]
BssSI 781 CACGAG CACGAG(-5/-1)
Cfr10I 318 GCCGGT [GA]^CCGG[CT]
DpnII 667 GATC ^GATC
DraIII 598 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 55 GACGCTCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1298 CCGTGG C^C[GA][CT]GG
EcoRI 637 GAATTC G^AATTC
FspI 1388 TGCGCA TGC^GCA
KpnI 941 GGTACC GGTAC^C
MboI 667 GATC ^GATC
MluNI 1232 TGGCCA TGG^CCA
MscI 1232 TGGCCA TGG^CCA
MslI 1083 CACCAGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII 667 GATC ^GATC
PflMI 792 CCACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 774 CACGTG CAC^GTG
PmlI 774 CACGTG CAC^GTG
PshAI 880 GACCTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 784 GAGCTC GAGCT^C
Sau3AI 667 GATC ^GATC
SbfI 711 CCTGCAGG CCTGCA^GG
SexAI 1277 ACCTGGT A^CC[AT]GGT
SmaI 41 CCCGGG CCC^GGG
Sse8387I 711 CCTGCAGG CCTGCA^GG
Tsp509I 638 AATT ^AATT
Van91I 792 CCACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 41 CCCGGG C^CCGGG
XmaI 41 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcyI 607 GGCGTC G[GA]^CG[CT]C
1303 GACGCC
AfaI 549 GTAC GT^AC
942 GTAC
BanI 941 GGTACC G^G[CT][GA]CC
1081 GGCACC
BlpI 846 GCTCAGC GC^T[ACGT]AGC
1152 GCTGAGC
BpmI 457 CTCCAG CTGGAG(16/14)
564 CTGGAG
Bpu1102I 846 GCTCAGC GC^T[ACGT]AGC
1152 GCTGAGC
BsaHI 607 GGCGTC G[GA]^CG[CT]C
1303 GACGCC
BsmAI 948 GAGAC GTCTC(1/5)
64 GTCTC
BsrI 194 ACTGG ACTGG(1/-1)
1085 CCAGT
CelII 846 GCTCAGC GC^T[ACGT]AGC
1152 GCTGAGC
HaeII 658 AGCGCC [GA]GCGC^[CT]
1039 AGCGCC
Hin1I 607 GGCGTC G[GA]^CG[CT]C
1303 GACGCC
HphI 572 TCACC GGTGA(8/7)
1103 TCACC
MaeII 775 ACGT A^CGT
982 ACGT
NspI 912 GCATGT [GA]CATG^[CT]
1052 GCATGT
PpuMI 1274 AGGACCT [GA]G^G[AT]CC[CT]
1355 AGGACCC
RsaI 549 GTAC GT^AC
942 GTAC
SapI 123 GCTCTTC GCTCTTC(1/4)
159 GCTCTTC
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# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AccII AciI AluI AlwNI AocI
ApaI AvaI AvaII BanII BbvI
BcnI BmyI BsaJI BseRI BsgI
BsiYI BslI BsmFI BsoBI Bsp1286I
BssKI BstF5I BstNI BstUI Bsu36I
Cac8I CfoI Cfr13I DdeI DraII
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII ItaI Ksp632I MaeIII MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NlaIII NlaIV PleI
PstI PvuII Sau96I ScrFI SfaNI
SfcI StuI TaqI TseI Tsp45I
TspRI TthHB8I