(Circular) (Six-base) MAPSORT of: pFN21ASDA1950.seq Check: 3436 from: 1 to: 6223 pFN21ASDA1950 6223 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3298 3298 Size: 6223 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4033 278 Size: 53 83 186 936 2497 2468 Fragments arranged by size: 2497 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2035 3566 1937 Size: 98 1531 4594 Fragments arranged by size: 4594 1531 98 Acc65I G'GTAC_C Cuts at: 2964 3544 2964 Size: 580 5643 AclI AA'CG_TT Cuts at: 1563 4350 4723 1563 Size: 2787 373 3063 Fragments arranged by size: 3063 2787 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2584 2746 4286 5670 5938 1632 Size: 952 162 1540 1384 268 1917 Fragments arranged by size: 1917 1540 1384 952 268 162 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6026 AflIII A'CryG_T Cuts at: 1203 5122 6099 1203 Size: 3919 977 1327 Fragments arranged by size: 3919 1327 977 AgeI A'CCGG_T Cuts at: 5054 5054 Size: 6223 AhdI GACnn_n'nnGTC Cuts at: 4952 4952 Size: 6223 AleI CACnn'nnGTG Cuts at: 1166 3110 1166 Size: 1944 4279 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6223 AlwNI CAG_nnn'CTG Cuts at: 2113 2748 3274 3966 5538 2113 Size: 635 526 692 1572 2798 Fragments arranged by size: 2798 1572 692 635 526 ApaI G_GGCC'C Cuts at: 2064 2679 2755 3221 2064 Size: 615 76 466 5066 Fragments arranged by size: 5066 615 466 76 ApaLI G'TGCA_C Cuts at: 4280 5436 4280 Size: 1156 5067 ApoI r'AATT_y Cuts at: 1493 2660 3532 3758 3910 6019 1493 Size: 1167 872 226 152 2109 1697 Fragments arranged by size: 2109 1697 1167 872 226 152 AseI AT'TA_AT Cuts at: 160 1032 4777 160 Size: 872 3745 1606 Fragments arranged by size: 3745 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6223 AvaI C'yCGr_G Cuts at: 1870 1943 2064 2234 2243 2723 3070 3548 1870 Size: 73 121 170 9 480 347 478 4545 Fragments arranged by size: 4545 480 478 347 170 121 73 9 BamHI G'GATC_C Cuts at: 3553 3920 3553 Size: 367 5856 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2964 3104 3544 4999 618 Size: 325 217 627 1177 140 440 1455 1842 Fragments arranged by size: 1842 1455 1177 627 440 325 217 140 BanII G_rGCy'C Cuts at: 1391 2064 2679 2755 2811 3102 3123 3221 3542 Size: 673 615 76 56 291 21 98 321 Cuts at: 3542 1391 Size: 4072 Fragments arranged by size: 4072 673 615 321 291 98 76 56 21 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5252 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2194 2228 4414 4448 1903 Size: 34 257 34 2186 34 3678 Fragments arranged by size: 3678 2186 257 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4115 5325 4115 Size: 1210 5013 BclI T'GATC_A Cuts at: 1548 1548 Size: 6223 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3197 4834 136 Size: 107 122 71 631 605 1525 1637 1525 Fragments arranged by size: 1637 1525 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6218 6218 Size: 6223 BlpI GC'TnA_GC Cuts at: 2870 3176 3640 2870 Size: 306 464 5453 Fragments arranged by size: 5453 464 306 Bme1580I G_kGCm'C Cuts at: 1790 2064 2622 2679 2755 2770 3221 3463 4284 Size: 274 558 57 76 15 451 242 821 Cuts at: 4284 5440 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 821 558 451 274 242 76 57 15 BmgBI CAC'GTC Cuts at: 1318 3006 1318 Size: 1688 4535 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3954 4912 442 Size: 416 53 83 399 24 2537 958 1753 Fragments arranged by size: 2537 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6223 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3109 3109 Size: 6223 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2465 2608 4883 1239 Size: 1226 143 2275 2579 Fragments arranged by size: 2579 2275 1226 143 Bpu10I CC'TnA_GC Cuts at: 2225 2330 2225 Size: 105 6118 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4348 5213 5511 4348 Size: 865 298 5060 Fragments arranged by size: 5060 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2092 4886 882 Size: 617 593 2794 2219 Fragments arranged by size: 2794 2219 617 593 BsaAI yAC'GTr Cuts at: 493 1204 2799 3520 4014 493 Size: 711 1595 721 494 2702 Fragments arranged by size: 2702 1595 721 711 494 BsaBI GATnn'nnATC Cuts at: 6217 6217 Size: 6223 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2631 3327 4030 4412 Size: 53 83 186 936 1098 696 703 382 Cuts at: 4412 5047 275 Size: 635 1451 Fragments arranged by size: 1451 1098 936 703 696 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2280 4656 5054 5328 5475 5886 2280 Size: 2376 398 274 147 411 2617 Fragments arranged by size: 2617 2376 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3419 3449 1178 Size: 30 165 30 2016 30 3952 Fragments arranged by size: 3952 2016 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2351 2440 3308 3427 3460 1186 Size: 1165 89 868 119 33 3949 Fragments arranged by size: 3949 1165 868 119 89 33 BseYI C'CCAG_C Cuts at: 2459 2548 2678 2748 3011 3339 3386 3421 5426 Size: 89 130 70 263 328 47 35 2005 Cuts at: 5426 5922 2459 Size: 496 2760 Fragments arranged by size: 2760 2005 496 328 263 130 89 70 47 35 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2173 2345 2362 2392 3326 1163 Size: 573 437 172 17 30 934 4060 Fragments arranged by size: 4060 934 573 437 172 30 17 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3337 3673 4434 5462 1540 Size: 275 186 1336 336 761 1028 2301 Fragments arranged by size: 2301 1336 1028 761 336 275 186 BsiHKAI G_wGCw'C Cuts at: 2811 4284 4369 5440 2811 Size: 1473 85 1071 3594 Fragments arranged by size: 3594 1473 1071 85 BsmI GAATG_Cn' Cuts at: 3734 3827 3734 Size: 93 6130 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2064 2622 2679 2755 2770 2811 3102 Size: 399 274 558 57 76 15 41 291 Cuts at: 3102 3123 3221 3463 3542 4284 4369 5440 1391 Size: 21 98 242 79 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 558 399 291 274 242 98 85 79 76 57 41 21 15 BspEI T'CCGG_A Cuts at: 5886 5886 Size: 6223 BspHI T'CATG_A Cuts at: 4112 5842 4112 Size: 1730 4493 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3298 3578 844 Size: 2454 280 3489 Fragments arranged by size: 3489 2454 280 BsrBI CCG'CTC Cuts at: 1394 1823 2143 4110 1394 Size: 429 320 1967 3507 Fragments arranged by size: 3507 1967 429 320 BsrDI GCAATG_nn' Cuts at: 65 1248 4718 4892 65 Size: 1183 3470 174 1396 Fragments arranged by size: 3470 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2341 4867 5054 1700 Size: 110 531 2526 187 2869 Fragments arranged by size: 2869 2526 531 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6223 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6223 BssSI C'ACGA_G Cuts at: 2804 4283 5295 2804 Size: 1479 1012 3732 Fragments arranged by size: 3732 1479 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6223 BstBI TT'CG_AA Cuts at: 2007 5027 2007 Size: 3020 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6223 BstYI r'GATC_y Cuts at: 1972 3553 3920 4305 4322 5774 5860 5872 6218 Size: 1581 367 385 17 1452 86 12 346 Cuts at: 6218 1972 Size: 1977 Fragments arranged by size: 1977 1581 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2921 3157 3443 2921 Size: 236 286 5701 Fragments arranged by size: 5701 286 236 BtgI C'CryG_G Cuts at: 513 1065 3321 513 Size: 552 2256 3415 Fragments arranged by size: 3415 2256 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5484 Fragments arranged by size: 5484 683 56 BtsI GCAGTG_nn' Cuts at: 2036 3739 4533 4553 2036 Size: 1703 794 20 3706 Fragments arranged by size: 3706 1703 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6223 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6188 DraI TTT'AAA Cuts at: 3527 3527 Size: 6223 DraIII CAC_nnn'GTG Cuts at: 2626 2626 Size: 6223 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2084 5230 809 Size: 507 768 3146 1802 Fragments arranged by size: 3146 1802 768 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2154 3255 3366 Size: 54 1425 50 275 18 324 1101 111 Cuts at: 3366 3670 4559 6188 8 Size: 304 889 1629 43 Fragments arranged by size: 1629 1425 1101 889 324 304 275 111 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3670 1537 Size: 275 1858 4090 Fragments arranged by size: 4090 1858 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2153 2189 3504 4153 1353 Size: 800 36 1315 649 3423 Fragments arranged by size: 3423 1315 800 649 36 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2113 4810 5184 5330 387 Size: 1064 138 524 2697 374 146 1280 Fragments arranged by size: 2697 1280 1064 524 374 146 138 EcoICRI GAG'CTC Cuts at: 2809 2809 Size: 6223 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2465 2584 2608 2746 4286 4883 5670 Size: 393 833 119 24 138 1540 597 787 Cuts at: 5670 5938 1239 Size: 268 1524 Fragments arranged by size: 1540 1524 833 787 597 393 268 138 119 24 EcoNI CCTnn'n_nnAGG Cuts at: 2156 2333 2702 2858 2879 3305 3442 2156 Size: 177 369 156 21 426 137 4937 Fragments arranged by size: 4937 426 369 177 156 137 21 EcoO109I rG'GnC_Cy Cuts at: 2061 2675 2728 2752 2884 3217 3218 3271 3298 Size: 614 53 24 132 333 1 53 27 Cuts at: 3298 3379 2061 Size: 81 4905 Fragments arranged by size: 4905 614 333 132 81 53 53 27 24 1 EcoRI G'AATT_C Cuts at: 2660 3532 3910 2660 Size: 872 378 4973 Fragments arranged by size: 4973 872 378 FspI TGC'GCA Cuts at: 3413 4729 3413 Size: 1316 4907 HaeII r_GCGC'y Cuts at: 1678 2685 3066 5370 1678 Size: 1007 381 2304 2531 Fragments arranged by size: 2531 2304 1007 381 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3419 3451 4864 4896 4938 Size: 32 135 32 1847 32 1413 32 42 Cuts at: 4938 4970 1373 Size: 32 2626 Fragments arranged by size: 2626 1847 1413 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3567 3813 669 Size: 988 281 1629 246 3079 Fragments arranged by size: 3079 1629 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6223 HpaI GTT'AAC Cuts at: 3813 3813 Size: 6223 KpnI G_GTAC'C Cuts at: 2968 3548 2968 Size: 580 5643 MfeI C'AATT_G Cuts at: 3822 3822 Size: 6223 MluI A'CGCG_T Cuts at: 6099 6099 Size: 6223 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3096 5337 5521 1305 Size: 1791 2241 184 2007 Fragments arranged by size: 2241 2007 1791 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3257 10 Size: 54 1425 343 1425 2976 Fragments arranged by size: 2976 1425 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3110 4181 4540 4699 518 Size: 648 1944 1071 359 159 2042 Fragments arranged by size: 2042 1944 1071 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2113 2368 2376 2445 2841 2850 3205 3280 Size: 507 255 8 69 396 9 355 75 Cuts at: 3280 3339 3639 4316 5464 5709 1606 Size: 59 300 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 507 396 355 300 255 245 75 69 59 9 8 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5671 NdeI CA'TA_TG Cuts at: 387 387 Size: 6223 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6223 NotI GC'GGCC_GC Cuts at: 3670 3670 Size: 6223 NspI r_CATG'y Cuts at: 1132 1229 2939 3079 3581 5126 1132 Size: 97 1710 140 502 1545 2229 Fragments arranged by size: 2229 1710 1545 502 140 97 PasI CC'CwG_GG Cuts at: 1348 2770 3498 1348 Size: 1422 728 4073 Fragments arranged by size: 4073 1422 728 PciI A'CATG_T Cuts at: 5122 5122 Size: 6223 PflMI CCAn_nnn'nTGG Cuts at: 1108 2821 1108 Size: 1713 4510 PmeI GTTT'AAAC Cuts at: 3527 3527 Size: 6223 PmlI CAC'GTG Cuts at: 2799 2799 Size: 6223 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4292 4324 5805 5837 4292 Size: 32 1481 32 4678 Fragments arranged by size: 4678 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3298 3379 3298 Size: 81 6142 PshAI GACnn'nnGTC Cuts at: 1613 2907 1613 Size: 1294 4929 PsiI TTA'TAA Cuts at: 3793 3793 Size: 6223 PspOMI G'GGCC_C Cuts at: 2060 2675 2751 3217 2060 Size: 615 76 466 5066 Fragments arranged by size: 5066 615 466 76 PstI C_TGCA'G Cuts at: 830 2031 2109 2277 2639 2739 2934 3279 3575 Size: 1201 78 168 362 100 195 345 296 Cuts at: 3575 830 Size: 3478 Fragments arranged by size: 3478 1201 362 345 296 195 168 100 78 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6223 PvuII CAG'CTG Cuts at: 2113 2368 2376 2113 Size: 255 8 5960 Fragments arranged by size: 5960 255 8 SacI G_AGCT'C Cuts at: 2811 2811 Size: 6223 SalI G'TCGA_C Cuts at: 1936 3565 1936 Size: 1629 4594 SapI GCTCTTCn'nnn_ Cuts at: 2153 2189 2153 Size: 36 6187 SbfI CC_TGCA'GG Cuts at: 2739 3575 2739 Size: 836 5387 ScaI AGT'ACT Cuts at: 4471 6183 4471 Size: 1712 4511 SexAI A'CCwGG_T Cuts at: 3300 3300 Size: 6223 SfcI C'TryA_G Cuts at: 826 1045 2027 2105 2273 2315 2635 2735 2930 Size: 219 982 78 168 42 320 100 195 Cuts at: 2930 3275 3571 4706 5387 5578 826 Size: 345 296 1135 681 191 1471 Fragments arranged by size: 1471 1135 982 681 345 320 296 219 195 191 168 100 78 42 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6223 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6223 SmaI CCC'GGG Cuts at: 1872 2066 3550 1872 Size: 194 1484 4545 Fragments arranged by size: 4545 1484 194 SmlI C'TyrA_G Cuts at: 820 1017 1943 4327 5228 5490 820 Size: 197 926 2384 901 262 1553 Fragments arranged by size: 2384 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3520 4014 493 Size: 3027 494 2702 Fragments arranged by size: 3027 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6223 SphI G_CATG'C Cuts at: 1132 3581 1132 Size: 2449 3774 SspI AAT'ATT Cuts at: 5 52 4147 5 Size: 47 4095 2081 Fragments arranged by size: 4095 2081 47 StuI AGG'CCT Cuts at: 1517 2162 2387 2585 1517 Size: 645 225 198 5155 Fragments arranged by size: 5155 645 225 198 StyI C'CwwG_G Cuts at: 513 1065 3662 513 Size: 552 2597 3074 Fragments arranged by size: 3074 2597 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3400 4250 4267 4420 4605 5050 325 Size: 1255 1820 850 17 153 185 445 1498 Fragments arranged by size: 1820 1498 1255 850 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4469 6181 96 Size: 274 80 33 51 1443 2492 1712 138 Fragments arranged by size: 2492 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2525 2557 3442 3474 6183 6215 2525 Size: 32 885 32 2709 32 2533 Fragments arranged by size: 2709 2533 885 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6223 XbaI T'CTAG_A Cuts at: 3559 3559 Size: 6223 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6223 XmaI C'CCGG_G Cuts at: 1870 2064 3548 1870 Size: 194 1484 4545 Fragments arranged by size: 4545 1484 194 XmnI GAAnn'nnTTC Cuts at: 4352 4352 Size: 6223 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4031 276 Size: 53 83 186 936 2497 2468 Fragments arranged by size: 2497 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI XcmI