Restriction map for
pFN21ASDA1950
(Linear) (Six-base) MAPSORT of: KIAA1950.seq Check: 5781 from: 1 to: 1494
KIAA1950.orf
With 182 enzymes: SgfI *
February 28, 2007 11:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1275 1494
Size: 1275 219
AccI GT'mk_AC
Cuts at: 0 12 1494
Size: 12 1482
Acc65I G'GTAC_C
Cuts at: 0 941 1494
Size: 941 553
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 561 723 1494
Size: 561 162 771
Fragments arranged by size:
771 561 162
AleI CACnn'nnGTG
Cuts at: 0 1087 1494
Size: 1087 407
AlwNI CAG_nnn'CTG
Cuts at: 0 90 725 1251 1494
Size: 90 635 526 243
Fragments arranged by size:
635 526 243 90
ApaI G_GGCC'C
Cuts at: 0 41 656 732 1198 1494
Size: 41 615 76 466 296
Fragments arranged by size:
615 466 296 76 41
ApoI r'AATT_y
Cuts at: 0 637 1494
Size: 637 857
AvaI C'yCGr_G
Cuts at: 0 41 211 220 700 1047 1494
Size: 41 170 9 480 347 447
Fragments arranged by size:
480 447 347 170 41 9
BanI G'GyrC_C
Cuts at: 0 941 1081 1494
Size: 941 140 413
Fragments arranged by size:
941 413 140
BanII G_rGCy'C
Cuts at: 0 41 656 732 788 1079 1100 1198 1494
Size: 41 615 76 56 291 21 98 296
Fragments arranged by size:
615 296 291 98 76 56 41 21
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 171 205 1494
Size: 171 34 1289
Fragments arranged by size:
1289 171 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1174 1494
Size: 1174 320
BlpI GC'TnA_GC
Cuts at: 0 847 1153 1494
Size: 847 306 341
Fragments arranged by size:
847 341 306
Bme1580I G_kGCm'C
Cuts at: 0 41 599 656 732 747 1198 1440 1494
Size: 41 558 57 76 15 451 242 54
Fragments arranged by size:
558 451 242 76 57 54 41 15
BmgBI CAC'GTC
Cuts at: 0 983 1494
Size: 983 511
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1086 1494
Size: 1086 408
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 442 585 1494
Size: 442 143 909
Fragments arranged by size:
909 442 143
Bpu10I CC'TnA_GC
Cuts at: 0 202 307 1494
Size: 202 105 1187
Fragments arranged by size:
1187 202 105
BsaI GGTCTCn'nnnn_
Cuts at: 0 69 1494
Size: 69 1425
BsaAI yAC'GTr
Cuts at: 0 776 1494
Size: 776 718
BsaHI Gr'CG_yC
Cuts at: 0 608 1304 1494
Size: 608 696 190
Fragments arranged by size:
696 608 190
BsaWI w'CCGG_w
Cuts at: 0 257 1494
Size: 257 1237
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1396 1426 1494
Size: 1396 30 68
Fragments arranged by size:
1396 68 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 328 417 1285 1404 1437 1494
Size: 328 89 868 119 33 57
Fragments arranged by size:
868 328 119 89 57 33
BseYI C'CCAG_C
Cuts at: 0 436 525 655 725 988 1316 1363 1398
Size: 436 89 130 70 263 328 47 35
Cuts at: 1398 1494
Size: 96
Fragments arranged by size:
436 328 263 130 96 89 70 47
35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 150 322 339 369 1303 1494
Size: 150 172 17 30 934 191
Fragments arranged by size:
934 191 172 150 30 17
BsiEI CG_ry'CG
Cuts at: 0 1314 1494
Size: 1314 180
BsiHKAI G_wGCw'C
Cuts at: 0 788 1494
Size: 788 706
Bsp1286I G_dGCh'C
Cuts at: 0 41 599 656 732 747 788 1079 1100
Size: 41 558 57 76 15 41 291 21
Cuts at: 1100 1198 1440 1494
Size: 98 242 54
Fragments arranged by size:
558 291 242 98 76 57 54 41
41 21 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1275 1494
Size: 1275 219
BsrBI CCG'CTC
Cuts at: 0 120 1494
Size: 120 1374
BsrFI r'CCGG_y
Cuts at: 0 318 1494
Size: 318 1176
BssSI C'ACGA_G
Cuts at: 0 781 1494
Size: 781 713
Bsu36I CC'TnA_GG
Cuts at: 0 898 1134 1420 1494
Size: 898 236 286 74
Fragments arranged by size:
898 286 236 74
BtgI C'CryG_G
Cuts at: 0 1298 1494
Size: 1298 196
BtsI GCAGTG_nn'
Cuts at: 0 13 1494
Size: 13 1481
DraIII CAC_nnn'GTG
Cuts at: 0 603 1494
Size: 603 891
DrdI GACnn_nn'nnGTC
Cuts at: 0 61 1494
Size: 61 1433
EaeI y'GGCC_r
Cuts at: 0 131 1232 1343 1494
Size: 131 1101 111 151
Fragments arranged by size:
1101 151 131 111
EarI CTCTTCn'nnn_
Cuts at: 0 130 166 1481 1494
Size: 130 36 1315 13
Fragments arranged by size:
1315 130 36 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 90 1494
Size: 90 1404
EcoICRI GAG'CTC
Cuts at: 0 786 1494
Size: 786 708
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 442 561 585 723 1494
Size: 442 119 24 138 771
Fragments arranged by size:
771 442 138 119 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 133 310 679 835 856 1282 1419 1494
Size: 133 177 369 156 21 426 137 75
Fragments arranged by size:
426 369 177 156 137 133 75 21
EcoO109I rG'GnC_Cy
Cuts at: 0 38 652 705 729 861 1194 1195 1248
Size: 38 614 53 24 132 333 1 53
Cuts at: 1248 1275 1356 1494
Size: 27 81 138
Fragments arranged by size:
614 333 138 132 81 53 53 38
27 24 1
EcoRI G'AATT_C
Cuts at: 0 637 1494
Size: 637 857
FspI TGC'GCA
Cuts at: 0 1390 1494
Size: 1390 104
HaeII r_GCGC'y
Cuts at: 0 662 1043 1494
Size: 662 381 451
Fragments arranged by size:
662 451 381
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1396 1428 1494
Size: 1396 32 66
Fragments arranged by size:
1396 66 32
KpnI G_GTAC'C
Cuts at: 0 945 1494
Size: 945 549
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1073 1494
Size: 1073 421
MscI TGG'CCA
Cuts at: 0 1234 1494
Size: 1234 260
MslI CAynn'nnrTG
Cuts at: 0 1087 1494
Size: 1087 407
MspA1I CmG'CkG
Cuts at: 0 90 345 353 422 818 827 1182 1257
Size: 90 255 8 69 396 9 355 75
Cuts at: 1257 1316 1494
Size: 59 178
Fragments arranged by size:
396 355 255 178 90 75 69 59
9 8
NspI r_CATG'y
Cuts at: 0 916 1056 1494
Size: 916 140 438
Fragments arranged by size:
916 438 140
PasI CC'CwG_GG
Cuts at: 0 747 1475 1494
Size: 747 728 19
Fragments arranged by size:
747 728 19
PflMI CCAn_nnn'nTGG
Cuts at: 0 798 1494
Size: 798 696
PmlI CAC'GTG
Cuts at: 0 776 1494
Size: 776 718
PpuMI rG'GwC_Cy
Cuts at: 0 1275 1356 1494
Size: 1275 81 138
Fragments arranged by size:
1275 138 81
PshAI GACnn'nnGTC
Cuts at: 0 884 1494
Size: 884 610
PspOMI G'GGCC_C
Cuts at: 0 37 652 728 1194 1494
Size: 37 615 76 466 300
Fragments arranged by size:
615 466 300 76 37
PstI C_TGCA'G
Cuts at: 0 8 86 254 616 716 911 1256 1494
Size: 8 78 168 362 100 195 345 238
Fragments arranged by size:
362 345 238 195 168 100 78 8
PvuII CAG'CTG
Cuts at: 0 90 345 353 1494
Size: 90 255 8 1141
Fragments arranged by size:
1141 255 90 8
SacI G_AGCT'C
Cuts at: 0 788 1494
Size: 788 706
SapI GCTCTTCn'nnn_
Cuts at: 0 130 166 1494
Size: 130 36 1328
Fragments arranged by size:
1328 130 36
SbfI CC_TGCA'GG
Cuts at: 0 716 1494
Size: 716 778
SexAI A'CCwGG_T
Cuts at: 0 1277 1494
Size: 1277 217
SfcI C'TryA_G
Cuts at: 0 4 82 250 292 612 712 907 1252
Size: 4 78 168 42 320 100 195 345
Cuts at: 1252 1494
Size: 242
Fragments arranged by size:
345 320 242 195 168 100 78 42
4
SmaI CCC'GGG
Cuts at: 0 43 1494
Size: 43 1451
StuI AGG'CCT
Cuts at: 0 139 364 562 1494
Size: 139 225 198 932
Fragments arranged by size:
932 225 198 139
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1377 1494
Size: 1377 117
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 502 534 1419 1451 1494
Size: 502 32 885 32 43
Fragments arranged by size:
885 502 43 32 32
XmaI C'CCGG_G
Cuts at: 0 41 1494
Size: 41 1453
Enzymes that do cut:
AarI AccI Acc65I AcuI AleI AlwNI ApaI ApoI
AvaI BanI BanII BcgI BglI BlpI Bme1580I BmgBI
BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI
BsrFI BssSI Bsu36I BtgI BtsI DraIII DrdI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I KpnI MmeI MscI MslI MspA1I NspI
PasI PflMI PmlI PpuMI PshAI PspOMI PstI PvuII
SacI SapI SbfI SexAI SfcI SmaI StuI TaqII
TstI XmaI
Enzymes that do not cut:
AatII AclI AfeI AflII AflIII AgeI AhdI AlfI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbsI BbvCI BciVI BclI BfrBI BglII BmrI
BmtI BpuEI BsaBI BsiWI BsmI BsmBI BspEI BspHI
BsrDI BsrGI BssHII BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgZI ClaI CspCI DraI EagI EcoRV FalI
FseI FspAI HincII HindIII HpaI KasI MfeI MluI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PfoI PmeI PpiI PsiI PspXI
PsrI PvuI RsrII SacII SalI SanDI ScaI SfiI
SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SspI StyI SwaI TatI Tth111I XbaI XcmI XhoI
XmnI ZraI