(Circular) (Six-base) MAPSORT of: pFN21ASDA1819.seq Check: 6550 from: 1 to: 8188 pFN21ASDA1819 8188 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2442 4993 2442 Size: 2551 5637 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4955 5998 278 Size: 53 83 186 936 3419 1043 2468 Fragments arranged by size: 3419 2468 1043 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4562 5531 1937 Size: 2625 969 4594 Fragments arranged by size: 4594 2625 969 Acc65I G'GTAC_C Cuts at: 5509 5509 Size: 8188 AclI AA'CG_TT Cuts at: 1563 6315 6688 1563 Size: 4752 373 3063 Fragments arranged by size: 4752 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3222 4055 4073 4650 6251 7635 7903 1632 Size: 1590 833 18 577 1601 1384 268 1917 Fragments arranged by size: 1917 1601 1590 1384 833 577 268 18 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7991 AflIII A'CryG_T Cuts at: 1203 3561 7087 8064 1203 Size: 2358 3526 977 1327 Fragments arranged by size: 3526 2358 1327 977 AgeI A'CCGG_T Cuts at: 7019 7019 Size: 8188 AhdI GACnn_n'nnGTC Cuts at: 6917 6917 Size: 8188 AleI CACnn'nnGTG Cuts at: 1166 5322 1166 Size: 4156 4032 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4470 1184 Size: 3286 4902 AlwNI CAG_nnn'CTG Cuts at: 2251 2434 2947 4345 5011 5423 5931 7503 2251 Size: 183 513 1398 666 412 508 1572 2936 Fragments arranged by size: 2936 1572 1398 666 513 508 412 183 ApaI G_GGCC'C Cuts at: 3149 5372 3149 Size: 2223 5965 ApaLI G'TGCA_C Cuts at: 2117 6245 7401 2117 Size: 4128 1156 2904 Fragments arranged by size: 4128 2904 1156 ApoI r'AATT_y Cuts at: 1493 2637 4828 5497 5723 5875 7984 1493 Size: 1144 2191 669 226 152 2109 1697 Fragments arranged by size: 2191 2109 1697 1144 669 226 152 AseI AT'TA_AT Cuts at: 160 1032 6742 160 Size: 872 5710 1606 Fragments arranged by size: 5710 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8188 AvaI C'yCGr_G Cuts at: 1870 1943 2110 5513 1870 Size: 73 167 3403 4545 Fragments arranged by size: 4545 3403 167 73 AvrII C'CTAG_G Cuts at: 3080 3080 Size: 8188 BamHI G'GATC_C Cuts at: 2929 5518 5885 2929 Size: 2589 367 5232 Fragments arranged by size: 5232 2589 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3594 5509 6964 618 Size: 325 217 627 1807 1915 1455 1842 Fragments arranged by size: 1915 1842 1807 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2088 2102 2149 2219 3149 3506 3675 3917 Size: 697 14 47 70 930 357 169 242 Cuts at: 3917 5372 5507 1391 Size: 1455 135 4072 Fragments arranged by size: 4072 1455 930 697 357 242 169 135 70 47 14 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 5420 928 Size: 971 3521 3696 Fragments arranged by size: 3696 3521 971 BbvCI CC'TCA_GC Cuts at: 3162 3167 3580 5345 3162 Size: 5 413 1765 6005 Fragments arranged by size: 6005 1765 413 5 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6379 6413 1903 Size: 34 4442 34 3678 Fragments arranged by size: 4442 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3322 4118 6080 7290 3322 Size: 796 1962 1210 4220 Fragments arranged by size: 4220 1962 1210 796 BclI T'GATC_A Cuts at: 1548 3009 3111 4881 1548 Size: 1461 102 1770 4855 Fragments arranged by size: 4855 1770 1461 102 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2078 3153 3301 Size: 107 122 71 631 605 406 1075 148 Cuts at: 3301 6799 136 Size: 3498 1525 Fragments arranged by size: 3498 1525 1075 631 605 406 148 122 107 71 BglII A'GATC_T Cuts at: 8183 8183 Size: 8188 BlpI GC'TnA_GC Cuts at: 3258 5174 5605 3258 Size: 1916 431 5841 Fragments arranged by size: 5841 1916 431 Bme1580I G_kGCm'C Cuts at: 1790 2121 3149 3597 5372 6249 7405 1790 Size: 331 1028 448 1775 877 1156 2573 Fragments arranged by size: 2573 1775 1156 1028 877 448 331 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8188 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4182 4511 4918 Size: 416 53 83 399 24 2765 329 407 Cuts at: 4918 5919 6877 442 Size: 1001 958 1753 Fragments arranged by size: 2765 1753 1001 958 416 407 399 329 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8188 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2674 3273 2674 Size: 599 7589 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2522 2934 3020 4316 4392 6848 1239 Size: 1283 412 86 1296 76 2456 2579 Fragments arranged by size: 2579 2456 1296 1283 412 86 76 Bpu10I CC'TnA_GC Cuts at: 2177 2252 2934 3162 3167 3580 4052 5345 2177 Size: 75 682 228 5 413 472 1293 5020 Fragments arranged by size: 5020 1293 682 472 413 228 75 5 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4902 6313 7178 7476 4902 Size: 1411 865 298 5614 Fragments arranged by size: 5614 1411 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4816 5086 6851 882 Size: 617 3317 270 1765 2219 Fragments arranged by size: 3317 2219 1765 617 270 BsaAI yAC'GTr Cuts at: 493 1204 5485 5979 493 Size: 711 4281 494 2702 Fragments arranged by size: 4281 2702 711 494 BsaBI GATnn'nnATC Cuts at: 8182 8182 Size: 8188 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4952 5261 5995 6377 Size: 53 83 186 936 3419 309 734 382 Cuts at: 6377 7012 275 Size: 635 1451 Fragments arranged by size: 3419 1451 936 734 635 382 309 186 83 53 BsaWI w'CCGG_w Cuts at: 3275 6621 7019 7293 7440 7851 3275 Size: 3346 398 274 147 411 3612 Fragments arranged by size: 3612 3346 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2320 2350 2761 2791 3211 Size: 30 165 30 917 30 411 30 420 Cuts at: 3211 3241 4118 4148 4226 4256 4314 4344 1178 Size: 30 877 30 78 30 58 30 5022 Fragments arranged by size: 5022 917 877 420 411 165 78 58 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3085 3150 3409 5049 1186 Size: 1899 65 259 1640 4325 Fragments arranged by size: 4325 1899 1640 259 65 BseYI C'CCAG_C Cuts at: 3321 3464 3661 4245 5264 7391 7887 3321 Size: 143 197 584 1019 2127 496 3622 Fragments arranged by size: 3622 2127 1019 584 496 197 143 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4884 1163 Size: 573 3148 4467 Fragments arranged by size: 4467 3148 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5638 6399 7427 1540 Size: 275 186 3637 761 1028 2301 Fragments arranged by size: 3637 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2121 2219 3077 3506 3917 6249 6334 7405 2121 Size: 98 858 429 411 2332 85 1071 2904 Fragments arranged by size: 2904 2332 1071 858 429 411 98 85 BsmI GAATG_Cn' Cuts at: 3191 4587 5699 5792 3191 Size: 1396 1112 93 5587 Fragments arranged by size: 5587 1396 1112 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2898 3231 2898 Size: 333 7855 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2088 2102 2121 2149 2219 3077 3149 Size: 399 298 14 19 28 70 858 72 Cuts at: 3149 3506 3597 3675 3917 5372 5507 6249 6334 Size: 357 91 78 242 1455 135 742 85 Cuts at: 6334 7405 1391 Size: 1071 2174 Fragments arranged by size: 2174 1455 1071 858 742 399 357 298 242 135 91 85 78 72 70 28 19 14 BspEI T'CCGG_A Cuts at: 3275 7851 3275 Size: 4576 3612 BspHI T'CATG_A Cuts at: 6077 7807 6077 Size: 1730 6458 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2442 4993 5543 844 Size: 1598 2551 550 3489 Fragments arranged by size: 3489 2551 1598 550 BsrBI CCG'CTC Cuts at: 1394 1823 6075 1394 Size: 429 4252 3507 Fragments arranged by size: 4252 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2791 4110 6683 6857 65 Size: 1183 1543 1319 2573 174 1396 Fragments arranged by size: 2573 1543 1396 1319 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3572 6832 7019 1700 Size: 110 1762 3260 187 2869 Fragments arranged by size: 3260 2869 1762 187 110 BsrGI T'GTAC_A Cuts at: 96 2260 3564 96 Size: 2164 1304 4720 Fragments arranged by size: 4720 2164 1304 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8188 BssSI C'ACGA_G Cuts at: 6248 7260 6248 Size: 1012 7176 BstAPI GCAn_nnn'nTGC Cuts at: 794 2434 4342 794 Size: 1640 1908 4640 Fragments arranged by size: 4640 1908 1640 BstBI TT'CG_AA Cuts at: 2007 6992 2007 Size: 4985 3203 BstEII G'GTnAC_C Cuts at: 1184 4056 1184 Size: 2872 5316 BstYI r'GATC_y Cuts at: 1972 2929 3628 4796 5518 5885 6270 6287 7739 Size: 957 699 1168 722 367 385 17 1452 Cuts at: 7739 7825 7837 8183 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1168 957 722 699 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3151 3624 4753 5144 3151 Size: 473 1129 391 6195 Fragments arranged by size: 6195 1129 473 391 BtgI C'CryG_G Cuts at: 513 1065 2022 3096 3198 3666 4739 5133 513 Size: 552 957 1074 102 468 1073 394 3568 Fragments arranged by size: 3568 1074 1073 957 552 468 394 102 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7449 Fragments arranged by size: 7449 683 56 BtsI GCAGTG_nn' Cuts at: 2313 2467 5009 5058 5088 5329 5704 6498 6518 Size: 154 2542 49 30 241 375 794 20 Cuts at: 6518 2313 Size: 3983 Fragments arranged by size: 3983 2542 794 375 241 154 49 30 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8188 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2437 2472 529 Size: 35 1873 35 6245 Fragments arranged by size: 6245 1873 35 35 DraI TTT'AAA Cuts at: 5492 5492 Size: 8188 DraIII CAC_nnn'GTG Cuts at: 4165 4165 Size: 8188 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7195 809 Size: 507 5879 1802 Fragments arranged by size: 5879 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2269 2863 3201 Size: 54 1425 50 275 18 439 594 338 Cuts at: 3201 3535 4742 5635 6524 8153 8 Size: 334 1207 893 889 1629 43 Fragments arranged by size: 1629 1425 1207 893 889 594 439 338 334 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5635 1537 Size: 275 3823 4090 Fragments arranged by size: 4090 3823 275 EarI CTCTTCn'nnn_ Cuts at: 1353 6118 1353 Size: 4765 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6775 7149 7295 387 Size: 1064 138 5186 374 146 1280 Fragments arranged by size: 5186 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2217 3504 3915 2217 Size: 1287 411 6490 Fragments arranged by size: 6490 1287 411 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2522 2934 3020 3222 4055 4073 4316 Size: 393 890 412 86 202 833 18 243 Cuts at: 4316 4392 4650 6251 6848 7635 7903 1239 Size: 76 258 1601 597 787 268 1524 Fragments arranged by size: 1601 1524 890 833 787 597 412 393 268 258 243 202 86 76 18 EcoNI CCTnn'n_nnAGG Cuts at: 3267 4752 3267 Size: 1485 6703 EcoO109I rG'GnC_Cy Cuts at: 2047 2070 2715 3084 3145 3146 3171 4184 5138 Size: 23 645 369 61 1 25 1013 954 Cuts at: 5138 5369 2047 Size: 231 4866 Fragments arranged by size: 4866 1013 954 645 369 231 61 25 23 1 EcoRI G'AATT_C Cuts at: 2637 5497 5875 2637 Size: 2860 378 4950 Fragments arranged by size: 4950 2860 378 EcoRV GAT'ATC Cuts at: 2629 2629 Size: 8188 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4062 4077 4062 Size: 15 8173 FseI GG_CCGG'CC Cuts at: 3576 3576 Size: 8188 FspI TGC'GCA Cuts at: 6694 6694 Size: 8188 HaeII r_GCGC'y Cuts at: 1678 2039 2140 7335 1678 Size: 361 101 5195 2531 Fragments arranged by size: 5195 2531 361 101 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2740 2772 2825 2857 4116 Size: 32 135 32 1168 32 53 32 1259 Cuts at: 4116 4138 4145 4148 4170 4177 6829 6861 6903 Size: 22 7 3 22 7 2652 32 42 Cuts at: 6903 6935 1373 Size: 32 2626 Fragments arranged by size: 2652 2626 1259 1168 135 53 42 32 32 32 32 32 32 22 22 7 7 3 HincII GTy'rAC Cuts at: 669 1657 1938 2569 5073 5532 5778 669 Size: 988 281 631 2504 459 246 3079 Fragments arranged by size: 3079 2504 988 631 459 281 246 HindIII A'AGCT_T Cuts at: 748 4486 748 Size: 3738 4450 HpaI GTT'AAC Cuts at: 2569 5073 5778 2569 Size: 2504 705 4979 Fragments arranged by size: 4979 2504 705 KpnI G_GTAC'C Cuts at: 5513 5513 Size: 8188 MfeI C'AATT_G Cuts at: 4268 4679 4706 4817 5787 4268 Size: 411 27 111 970 6669 Fragments arranged by size: 6669 970 411 111 27 MluI A'CGCG_T Cuts at: 8064 8064 Size: 8188 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4914 7302 7486 1305 Size: 3609 2388 184 2007 Fragments arranged by size: 3609 2388 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2271 2865 3203 10 Size: 54 1425 343 439 594 338 4995 Fragments arranged by size: 4995 1425 594 439 343 338 54 MslI CAynn'nnrTG Cuts at: 518 1166 2859 2972 3600 5322 6146 6505 6664 Size: 648 1693 113 628 1722 824 359 159 Cuts at: 6664 518 Size: 2042 Fragments arranged by size: 2042 1722 1693 824 648 628 359 159 113 MspA1I CmG'CkG Cuts at: 1606 3166 3182 3534 5604 6281 7429 7674 1606 Size: 1560 16 352 2070 677 1148 245 2120 Fragments arranged by size: 2120 2070 1560 1148 677 352 245 16 NaeI GCC'GGC Cuts at: 3574 3574 Size: 8188 NcoI C'CATG_G Cuts at: 513 1065 2022 3096 3198 3666 5133 513 Size: 552 957 1074 102 468 1467 3568 Fragments arranged by size: 3568 1467 1074 957 552 468 102 NdeI CA'TA_TG Cuts at: 387 387 Size: 8188 NgoMIV G'CCGG_C Cuts at: 3572 3572 Size: 8188 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8188 NotI GC'GGCC_GC Cuts at: 5635 5635 Size: 8188 NspI r_CATG'y Cuts at: 1132 1229 3362 3565 4855 5546 7091 1132 Size: 97 2133 203 1290 691 1545 2229 Fragments arranged by size: 2229 2133 1545 1290 691 203 97 PasI CC'CwG_GG Cuts at: 1348 2504 3586 1348 Size: 1156 1082 5950 Fragments arranged by size: 5950 1156 1082 PciI A'CATG_T Cuts at: 3561 7087 3561 Size: 3526 4662 PflMI CCAn_nnn'nTGG Cuts at: 1108 3182 4223 4507 4843 1108 Size: 2074 1041 284 336 4453 Fragments arranged by size: 4453 2074 1041 336 284 PmeI GTTT'AAAC Cuts at: 5492 5492 Size: 8188 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6257 6289 7770 7802 6257 Size: 32 1481 32 6643 Fragments arranged by size: 6643 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2715 5138 2715 Size: 2423 5765 PshAI GACnn'nnGTC Cuts at: 1613 3054 4393 1613 Size: 1441 1339 5408 Fragments arranged by size: 5408 1441 1339 PsiI TTA'TAA Cuts at: 4416 5758 4416 Size: 1342 6846 PspOMI G'GGCC_C Cuts at: 3145 5368 3145 Size: 2223 5965 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 5465 5497 5465 Size: 32 8156 PstI C_TGCA'G Cuts at: 830 2665 2943 4960 4990 5540 830 Size: 1835 278 2017 30 550 3478 Fragments arranged by size: 3478 2017 1835 550 278 30 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8188 PvuII CAG'CTG Cuts at: 3166 3182 3166 Size: 16 8172 SacI G_AGCT'C Cuts at: 2219 3506 3917 2219 Size: 1287 411 6490 Fragments arranged by size: 6490 1287 411 SalI G'TCGA_C Cuts at: 1936 5530 1936 Size: 3594 4594 SanDI GG'GwC_CC Cuts at: 5138 5138 Size: 8188 SbfI CC_TGCA'GG Cuts at: 5540 5540 Size: 8188 ScaI AGT'ACT Cuts at: 6436 8148 6436 Size: 1712 6476 SexAI A'CCwGG_T Cuts at: 3436 3436 Size: 8188 SfcI C'TryA_G Cuts at: 826 1045 2328 2661 2939 4292 4596 4956 4986 Size: 219 1283 333 278 1353 304 360 30 Cuts at: 4986 5536 6671 7352 7543 826 Size: 550 1135 681 191 1471 Fragments arranged by size: 1471 1353 1283 1135 681 550 360 333 304 278 219 191 30 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8188 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8188 SmaI CCC'GGG Cuts at: 1872 5515 1872 Size: 3643 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4917 6292 7193 7455 820 Size: 197 926 2974 1375 901 262 1553 Fragments arranged by size: 2974 1553 1375 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5485 5979 493 Size: 4992 494 2702 Fragments arranged by size: 4992 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 8188 SphI G_CATG'C Cuts at: 1132 3362 4855 5546 1132 Size: 2230 1493 691 3774 Fragments arranged by size: 3774 2230 1493 691 SspI AAT'ATT Cuts at: 5 52 2884 6112 5 Size: 47 2832 3228 2081 Fragments arranged by size: 3228 2832 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8188 StyI C'CwwG_G Cuts at: 513 1065 2022 2089 2301 3080 3096 3198 3666 Size: 552 957 67 212 779 16 102 468 Cuts at: 3666 5133 5627 513 Size: 1467 494 3074 Fragments arranged by size: 3074 1467 957 779 552 494 468 212 102 67 16 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3675 3797 5296 6215 6232 6385 6570 Size: 1255 2095 122 1499 919 17 153 185 Cuts at: 6570 7015 325 Size: 445 1498 Fragments arranged by size: 2095 1499 1498 1255 919 445 185 153 122 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2260 3564 6434 Size: 274 80 33 51 1443 283 1304 2870 Cuts at: 6434 8146 96 Size: 1712 138 Fragments arranged by size: 2870 1712 1443 1304 283 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3211 3243 8148 8180 3211 Size: 32 4905 32 3219 Fragments arranged by size: 4905 3219 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2697 2839 1316 Size: 1381 142 6665 Fragments arranged by size: 6665 1381 142 XbaI T'CTAG_A Cuts at: 5524 5524 Size: 8188 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2605 2687 4117 5120 2605 Size: 82 1430 1003 5673 Fragments arranged by size: 5673 1430 1003 82 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8188 XmaI C'CCGG_G Cuts at: 1870 5513 1870 Size: 3643 4545 XmnI GAAnn'nnTTC Cuts at: 6317 6317 Size: 8188 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4953 5996 276 Size: 53 83 186 936 3419 1043 2468 Fragments arranged by size: 3419 2468 1043 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BbeI BfrBI BsiWI BstXI BstZ17I FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI RsrII SacII SapI SfiI SfoI SrfI SwaI