Restriction map for
pFN21ASDA1769
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# NO CUT ENZYMES ( 45 enzymes) #
##################################
AclI AcsI AflII AhdI ApoI
AscI AseI AsnI AspEI BciVI
BfrI BsaBI BsiWI BsmI BspHI
BsrDI BstBI DraI Eam1105I EcoRI
EcoRV HindIII HpaI KspI MamI
MfeI MluI MunI NheI NotI
NruI NspV PacI PmeI PshBI
Psp1406I RcaI SacII SalI SfuI
SnaBI SpeI SspI SwaI XbaI
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# ONE CUT ENZYMES ( 24 enzymes) #
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AatII 2533 GACGTC GACGT^C
Asp700I 4791 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 705 TGCGCA TGC^GCA
AvrII 843 CCTAGG C^CTAGG
BclI 2684 TGATCA T^GATCA
BglII 1967 AGATCT A^GATCT
BlnI 843 CCTAGG C^CTAGG
BspLU11I 668 ACATGT A^CATGT
Bst1107I 4410 GTATAC GTA^TAC
BstZ17I 4410 GTATAC GTA^TAC
EcoT22I 5572 ATGCAT ATGCA^T
FbaI 2684 TGATCA T^GATCA
FspI 705 TGCGCA TGC^GCA
MseI 2888 TTAA T^TAA
NdeI 5548 CATATG CA^TATG
NsiI 5572 ATGCAT ATGCA^T
PshAI 2374 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 4835 CGATCG CGAT^CG
ScaI 149 AGTACT AGT^ACT
SgrAI 4155 CACCGGCG C[GA]^CCGG[CT]G
SphI 5570 GCATGC GCATG^C
Tru9I 2888 TTAA T^TAA
Tsp509I 2097 AATT ^AATT
XmnI 4791 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 32 enzymes) #
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AccI 3688 GTCTAC GT^[AC][GT]AC
4410 GTATAC
AccIII 2018 TCCGGA T^CCGGA
4527 TCCGGA
AgeI 4091 ACCGGT A^CCGGT
5513 ACCGGT
Aor51HI 3221 AGCGCT AGC^GCT
3806 AGCGCT
BamHI 3703 GGATCC G^GATCC
5150 GGATCC
BfaI 844 CTAG C^TAG
4621 CTAG
BseAI 2018 TCCGGA T^CCGGA
4527 TCCGGA
Bsp1407I 104 TGTACA T^GTACA
5024 TGTACA
BspDI 289 ATCGAT AT^CGAT
3400 ATCGAT
BspEI 2018 TCCGGA T^CCGGA
4527 TCCGGA
BsrGI 104 TGTACA T^GTACA
5024 TGTACA
BssHII 1659 GCGCGC G^CGCGC
3463 GCGCGC
BstXI 1724 CCATCCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5654 CCAGTGTCCTGG
ClaI 289 ATCGAT AT^CGAT
3400 ATCGAT
CpoI 110 CGGACCG CG^G[AT]CCG
2797 CGGTCCG
DraIII 214 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG
478 CACGAGGTG
Eco47III 3221 AGCGCT AGC^GCT
3806 AGCGCT
FseI 3652 GGCCGGCC GGCCGG^CC
4660 GGCCGGCC
HincII 4396 GTTGAC GT[CT]^[GA]AC
5368 GTTGAC
HindII 4396 GTTGAC GT[CT]^[GA]AC
5368 GTTGAC
MaeI 844 CTAG C^TAG
4621 CTAG
MroI 2018 TCCGGA T^CCGGA
4527 TCCGGA
PaeR7I 4273 CTCGAG C^TCGAG
4327 CTCGAG
PinAI 4091 ACCGGT A^CCGGT
5513 ACCGGT
RsrII 110 CGGACCG CG^G[AT]CCG
2797 CGGTCCG
SbfI 3190 CCTGCAGG CCTGCA^GG
3939 CCTGCAGG
SexAI 4818 ACCAGGT A^CC[AT]GGT
5237 ACCTGGT
SfiI 417 GGCCCGGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3652 GGCCGGCCGGGCC
Sse8387I 3190 CCTGCAGG CCTGCA^GG
3939 CCTGCAGG
SspBI 104 TGTACA T^GTACA
5024 TGTACA
TfiI 690 GAATC G^A[AT]TC
2880 GATTC
XhoI 4273 CTCGAG C^TCGAG
4327 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 157 enzymes) #
#########################################
Acc65I AccII AciI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
AocI ApaI ApaLI Asp718I AspHI
AspI AvaI AvaII BalI BanI
BanII BbeI BbrPI BbsI BbvCI
BbvI BcgI BcnI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BssSI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DrdI DsaI EaeI
EagI EarI EclXI Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
KpnI Ksp632I MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PflFI PflMI PleI PmaCI PmlI
PpuMI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SmlI StuI
StyI TaqI TseI Tsp45I TspRI
Tth111I TthHB8I Van91I XcmI XhoII
XmaCI XmaI