Restriction map for
pFN21ASDA1769
(Linear) (Six-base) MAPSORT of: KIAA1769.seq Check: 5962 from: 1 to: 6240
KIAA1769.orf
With 182 enzymes: SgfI *
February 28, 2007 11:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 931 3050 3273 3864 3933 4875 5320 6240
Size: 931 2119 223 591 69 942 445 920
Fragments arranged by size:
2119 942 931 920 591 445 223 69
AatII G_ACGT'C
Cuts at: 0 2537 6240
Size: 2537 3703
AccI GT'mk_AC
Cuts at: 0 3689 4411 6240
Size: 3689 722 1829
Fragments arranged by size:
3689 1829 722
Acc65I G'GTAC_C
Cuts at: 0 1676 3323 3981 4094 5531 6240
Size: 1676 1647 658 113 1437 709
Fragments arranged by size:
1676 1647 1437 709 658 113
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1897 2005 2632 3405 4002 4174 4284 5029
Size: 1897 108 627 773 597 172 110 745
Cuts at: 5029 5644 6240
Size: 615 596
Fragments arranged by size:
1897 773 745 627 615 597 596 172
110 108
AfeI AGC'GCT
Cuts at: 0 3223 3808 6240
Size: 3223 585 2432
Fragments arranged by size:
3223 2432 585
AflIII A'CryG_T
Cuts at: 0 668 1919 3296 6240
Size: 668 1251 1377 2944
Fragments arranged by size:
2944 1377 1251 668
AgeI A'CCGG_T
Cuts at: 0 4091 5513 6240
Size: 4091 1422 727
Fragments arranged by size:
4091 1422 727
AleI CACnn'nnGTG
Cuts at: 0 2245 2413 2699 4199 6240
Size: 2245 168 286 1500 2041
Fragments arranged by size:
2245 2041 1500 286 168
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2489 2717 3455 3979 5098 5155 6240
Size: 2489 228 738 524 1119 57 1085
Fragments arranged by size:
2489 1119 1085 738 524 228 57
AlwNI CAG_nnn'CTG
Cuts at: 0 309 755 828 933 1115 1468 2168 2525
Size: 309 446 73 105 182 353 700 357
Cuts at: 2525 3354 3504 3786 4025 4197 4307 5329 5640
Size: 829 150 282 239 172 110 1022 311
Cuts at: 5640 6240
Size: 600
Fragments arranged by size:
1022 829 700 600 446 357 353 311
309 282 239 182 172 150 110 105
73
ApaI G_GGCC'C
Cuts at: 0 420 1753 4083 4370 4816 6096 6240
Size: 420 1333 2330 287 446 1280 144
Fragments arranged by size:
2330 1333 1280 446 420 287 144
ApaLI G'TGCA_C
Cuts at: 0 812 916 2401 2925 4489 6240
Size: 812 104 1485 524 1564 1751
Fragments arranged by size:
1751 1564 1485 812 524 104
AvaI C'yCGr_G
Cuts at: 0 79 419 1120 1181 1399 1594 1669 1746
Size: 79 340 701 61 218 195 75 77
Cuts at: 1746 1840 2697 3245 3914 4083 4273 4327 4363
Size: 94 857 548 669 169 190 54 36
Cuts at: 4363 5099 6240
Size: 736 1141
Fragments arranged by size:
1141 857 736 701 669 548 340 218
195 190 169 94 79 77 75 61
54 36
AvrII C'CTAG_G
Cuts at: 0 843 6240
Size: 843 5397
BamHI G'GATC_C
Cuts at: 0 3703 5150 6240
Size: 3703 1447 1090
Fragments arranged by size:
3703 1447 1090
BanI G'GyrC_C
Cuts at: 0 212 258 352 799 1395 1676 1836 2214
Size: 212 46 94 447 596 281 160 378
Cuts at: 2214 2338 2701 2786 2833 3012 3323 3680 3955
Size: 124 363 85 47 179 311 357 275
Cuts at: 3955 3981 4008 4094 4246 4549 5531 6240
Size: 26 27 86 152 303 982 709
Fragments arranged by size:
982 709 596 447 378 363 357 311
303 281 275 212 179 160 152 124
94 86 85 47 46 27 26
BanII G_rGCy'C
Cuts at: 0 7 254 420 1312 1496 1670 1753 3379
Size: 7 247 166 892 184 174 83 1626
Cuts at: 3379 3410 3544 3991 4083 4370 4816 5098 5222
Size: 31 134 447 92 287 446 282 124
Cuts at: 5222 5837 6096 6107 6240
Size: 615 259 11 133
Fragments arranged by size:
1626 892 615 447 446 287 282 259
247 184 174 166 134 133 124 92
83 31 11 7
BbeI G_GCGC'C
Cuts at: 0 1840 2342 3684 4250 6240
Size: 1840 502 1342 566 1990
Fragments arranged by size:
1990 1840 1342 566 502
BbsI GAAGACnn'nnnn_
Cuts at: 0 498 1394 4837 5632 6240
Size: 498 896 3443 795 608
Fragments arranged by size:
3443 896 795 608 498
BbvCI CC'TCA_GC
Cuts at: 0 829 1454 2539 3992 4540 5039 5786 6240
Size: 829 625 1085 1453 548 499 747 454
Fragments arranged by size:
1453 1085 829 747 625 548 499 454
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 342 376 768 802 2282 2316 4308 4342
Size: 342 34 392 34 1480 34 1992 34
Cuts at: 4342 6240
Size: 1898
Fragments arranged by size:
1992 1898 1480 392 342 34 34 34
34
BclI T'GATC_A
Cuts at: 0 2684 6240
Size: 2684 3556
BfrBI ATG'CAT
Cuts at: 0 5574 6240
Size: 5574 666
BglI GCCn_nnn'nGGC
Cuts at: 0 424 885 1124 1411 1845 3374 3659 3893
Size: 424 461 239 287 434 1529 285 234
Cuts at: 3893 4233 5672 5920 6240
Size: 340 1439 248 320
Fragments arranged by size:
1529 1439 461 434 424 340 320 287
285 248 239 234
BglII A'GATC_T
Cuts at: 0 1967 6240
Size: 1967 4273
BlpI GC'TnA_GC
Cuts at: 0 121 907 937 6179 6240
Size: 121 786 30 5242 61
Fragments arranged by size:
5242 786 121 61 30
Bme1580I G_kGCm'C
Cuts at: 0 420 804 816 920 951 1400 1753 2219
Size: 420 384 12 104 31 449 353 466
Cuts at: 2219 2405 2432 2507 2929 3017 4083 4370 4493
Size: 186 27 75 422 88 1066 287 123
Cuts at: 4493 4552 4816 6096 6240
Size: 59 264 1280 144
Fragments arranged by size:
1280 1066 466 449 422 420 384 353
287 264 186 144 123 104 88 75
59 31 27 12
BmgBI CAC'GTC
Cuts at: 0 63 1392 2451 3333 3531 4104 5352 5630
Size: 63 1329 1059 882 198 573 1248 278
Cuts at: 5630 6240
Size: 610
Fragments arranged by size:
1329 1248 1059 882 610 573 278 198
63
BmrI ACTGGGnnnn_n'
Cuts at: 0 130 1002 1221 4452 4666 5050 5648 6240
Size: 130 872 219 3231 214 384 598 592
Fragments arranged by size:
3231 872 598 592 384 219 214 130
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3558 4194 5236 5241 6142 6240
Size: 3558 636 1042 5 901 98
Fragments arranged by size:
3558 1042 901 636 98 5
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 2218 3238 3412 3808 3925 4051 4165
Size: 331 1887 1020 174 396 117 126 114
Cuts at: 4165 4279 4729 4807 5211 5794 6240
Size: 114 450 78 404 583 446
Fragments arranged by size:
1887 1020 583 450 446 404 396 331
174 126 117 114 114 78
Bpu10I CC'TnA_GC
Cuts at: 0 253 829 1454 2539 3992 4540 5039 5086
Size: 253 576 625 1085 1453 548 499 47
Cuts at: 5086 5786 6240
Size: 700 454
Fragments arranged by size:
1453 1085 700 625 576 548 499 454
253 47
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2068 2229 2965 3475 6019 6240
Size: 2068 161 736 510 2544 221
Fragments arranged by size:
2544 2068 736 510 221 161
BsaI GGTCTCn'nnnn_
Cuts at: 0 1696 2366 5447 5522 6113 6240
Size: 1696 670 3081 75 591 127
Fragments arranged by size:
3081 1696 670 591 127 75
BsaAI yAC'GTr
Cuts at: 0 567 645 1922 3299 4038 4409 5706 6240
Size: 567 78 1277 1377 739 371 1297 534
Fragments arranged by size:
1377 1297 1277 739 567 534 371 78
BsaHI Gr'CG_yC
Cuts at: 0 533 679 974 1034 1508 1717 1837 2339
Size: 533 146 295 60 474 209 120 502
Cuts at: 2339 2534 2741 3681 3850 4216 4225 4247 4630
Size: 195 207 940 169 366 9 22 383
Cuts at: 4630 6240
Size: 1610
Fragments arranged by size:
1610 940 533 502 474 383 366 295
209 207 195 169 146 120 60 22
9
BsaWI w'CCGG_w
Cuts at: 0 2018 4091 4527 5147 5513 6240
Size: 2018 2073 436 620 366 727
Fragments arranged by size:
2073 2018 727 620 436 366
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1364 1394 1726 1756 3876 3906 6240
Size: 1364 30 332 30 2120 30 2334
Fragments arranged by size:
2334 2120 1364 332 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 80 846 1264 1734 2683 2692 4291 4639
Size: 80 766 418 470 949 9 1599 348
Cuts at: 4639 5658 5791 6240
Size: 1019 133 449
Fragments arranged by size:
1599 1019 949 766 470 449 418 348
133 80 9
BseYI C'CCAG_C
Cuts at: 0 748 755 1013 1195 1311 1866 2130 3363
Size: 748 7 258 182 116 555 264 1233
Cuts at: 3363 3497 3513 3568 3854 3921 4369 4696 4756
Size: 134 16 55 286 67 448 327 60
Cuts at: 4756 4923 6240
Size: 167 1317
Fragments arranged by size:
1317 1233 748 555 448 327 286 264
258 182 167 134 116 67 60 55
16 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 510 745 1834 3010 3028 3064 3457
Size: 427 83 235 1089 1176 18 36 393
Cuts at: 3457 3505 3892 3898 3967 4909 5125 5292 5342
Size: 48 387 6 69 942 216 167 50
Cuts at: 5342 5437 5728 6240
Size: 95 291 512
Fragments arranged by size:
1176 1089 942 512 427 393 387 291
235 216 167 95 83 69 50 48
36 18 6
BsiEI CG_ry'CG
Cuts at: 0 118 171 266 428 765 2289 2709 3237
Size: 118 53 95 162 337 1524 420 528
Cuts at: 3237 3658 4838 6240
Size: 421 1180 1402
Fragments arranged by size:
1524 1402 1180 528 421 420 337 162
118 95 53
BsiHKAI G_wGCw'C
Cuts at: 0 7 254 434 816 908 920 986 1496
Size: 7 247 180 382 92 12 66 510
Cuts at: 1496 2405 2929 3635 4037 4493 5222 5980 6240
Size: 909 524 706 402 456 729 758 260
Fragments arranged by size:
909 758 729 706 524 510 456 402
382 260 247 180 92 66 12 7
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1158 1367 3574 3757 6240
Size: 1158 209 2207 183 2483
Fragments arranged by size:
2483 2207 1158 209 183
Bsp1286I G_dGCh'C
Cuts at: 0 7 254 420 434 804 816 908 920
Size: 7 247 166 14 370 12 92 12
Cuts at: 920 951 986 1312 1400 1496 1670 1753 2219
Size: 31 35 326 88 96 174 83 466
Cuts at: 2219 2405 2432 2507 2929 3017 3379 3410 3544
Size: 186 27 75 422 88 362 31 134
Cuts at: 3544 3635 3991 4037 4083 4370 4493 4552 4816
Size: 91 356 46 46 287 123 59 264
Cuts at: 4816 5098 5222 5837 5980 6096 6107 6240
Size: 282 124 615 143 116 11 133
Fragments arranged by size:
615 466 422 370 362 356 326 287
282 264 247 186 174 166 143 134
133 124 123 116 96 92 91 88
88 83 75 59 46 46 35 31
31 27 14 12 12 11 7
BspEI T'CCGG_A
Cuts at: 0 2018 4527 6240
Size: 2018 2509 1713
Fragments arranged by size:
2509 2018 1713
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 375 699 931 1131 1637 1989 3050 3096
Size: 375 324 232 200 506 352 1061 46
Cuts at: 3096 3273 3311 3864 3933 4691 4875 5320 6240
Size: 177 38 553 69 758 184 445 920
Fragments arranged by size:
1061 920 758 553 506 445 375 352
324 232 200 184 177 69 46 38
BsrBI CCG'CTC
Cuts at: 0 630 1104 1420 1674 1713 4044 4713 5490
Size: 630 474 316 254 39 2331 669 777
Cuts at: 5490 5529 5721 6240
Size: 39 192 519
Fragments arranged by size:
2331 777 669 630 519 474 316 254
192 39 39
BsrFI r'CCGG_y
Cuts at: 0 113 261 2027 2078 2284 2514 2704 2718
Size: 113 148 1766 51 206 230 190 14
Cuts at: 2718 2783 3653 4091 4156 4466 4661 5288 5513
Size: 65 870 438 65 310 195 627 225
Cuts at: 5513 6199 6240
Size: 686 41
Fragments arranged by size:
1766 870 686 627 438 310 230 225
206 195 190 148 113 65 65 51
41 14
BsrGI T'GTAC_A
Cuts at: 0 104 5024 6240
Size: 104 4920 1216
Fragments arranged by size:
4920 1216 104
BssHII G'CGCG_C
Cuts at: 0 1659 3463 6240
Size: 1659 1804 2777
Fragments arranged by size:
2777 1804 1659
BssSI C'ACGA_G
Cuts at: 0 361 478 511 1891 2896 5536 5716 6190
Size: 361 117 33 1380 1005 2640 180 474
Cuts at: 6190 6240
Size: 50
Fragments arranged by size:
2640 1380 1005 474 361 180 117 50
33
BstAPI GCAn_nnn'nTGC
Cuts at: 0 438 2793 2924 3354 5329 6240
Size: 438 2355 131 430 1975 911
Fragments arranged by size:
2355 1975 911 438 430 131
BstEII G'GTnAC_C
Cuts at: 0 225 1551 3156 4641 6240
Size: 225 1326 1605 1485 1599
Fragments arranged by size:
1605 1599 1485 1326 225
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1731 5661 6240
Size: 1731 3930 579
Fragments arranged by size:
3930 1731 579
BstYI r'GATC_y
Cuts at: 0 872 1967 3350 3703 5150 5360 6240
Size: 872 1095 1383 353 1447 210 880
Fragments arranged by size:
1447 1383 1095 880 872 353 210
BstZ17I GTA'TAC
Cuts at: 0 4412 6240
Size: 4412 1828
Bsu36I CC'TnA_GG
Cuts at: 0 2156 5125 5641 6240
Size: 2156 2969 516 599
Fragments arranged by size:
2969 2156 599 516
BtgI C'CryG_G
Cuts at: 0 674 795 1037 1133 1274 1445 1480 2588
Size: 674 121 242 96 141 171 35 1108
Cuts at: 2588 2636 2735 3161 3369 3758 4346 4425 4908
Size: 48 99 426 208 389 588 79 483
Cuts at: 4908 5387 5738 6240
Size: 479 351 502
Fragments arranged by size:
1108 674 588 502 483 479 426 389
351 242 208 171 141 121 99 96
79 48 35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1979 4635 5829 5895 6240
Size: 1979 2656 1194 66 345
Fragments arranged by size:
2656 1979 1194 345 66
BtsI GCAGTG_nn'
Cuts at: 0 27 1385 1683 3022 3721 3933 6240
Size: 27 1358 298 1339 699 212 2307
Fragments arranged by size:
2307 1358 1339 699 298 212 27
ClaI AT'CG_AT
Cuts at: 0 290 3401 6240
Size: 290 3111 2839
Fragments arranged by size:
3111 2839 290
DraIII CAC_nnn'GTG
Cuts at: 0 219 483 6240
Size: 219 264 5757
Fragments arranged by size:
5757 264 219
DrdI GACnn_nn'nnGTC
Cuts at: 0 538 2746 5848 6240
Size: 538 2208 3102 392
Fragments arranged by size:
3102 2208 538 392
EaeI y'GGCC_r
Cuts at: 0 72 115 410 425 569 762 1244 1271
Size: 72 43 295 15 144 193 482 27
Cuts at: 1271 1477 1976 2274 2286 2417 2516 2553 2567
Size: 206 499 298 12 131 99 37 14
Cuts at: 2567 2604 2706 2753 3033 3234 3267 3479 3626
Size: 37 102 47 280 201 33 212 147
Cuts at: 3626 3655 3755 3811 3894 4151 4349 5384 5700
Size: 29 100 56 83 257 198 1035 316
Cuts at: 5700 5921 6201 6240
Size: 221 280 39
Fragments arranged by size:
1035 499 482 316 298 295 280 280
257 221 212 206 201 198 193 147
144 131 102 100 99 83 72 56
47 43 39 37 37 33 29 27
15 14 12
EagI C'GGCC_G
Cuts at: 0 115 425 762 2286 2706 3234 3655 6240
Size: 115 310 337 1524 420 528 421 2585
Fragments arranged by size:
2585 1524 528 421 420 337 310 115
EarI CTCTTCn'nnn_
Cuts at: 0 457 1873 1879 2089 2608 3568 4501 4526
Size: 457 1416 6 210 519 960 933 25
Cuts at: 4526 5086 5227 6240
Size: 560 141 1013
Fragments arranged by size:
1416 1013 960 933 560 519 457 210
141 25 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 172 596 2689 4243 6240
Size: 172 424 2093 1554 1997
Fragments arranged by size:
2093 1997 1554 424 172
EcoICRI GAG'CTC
Cuts at: 0 5 252 1494 5220 6240
Size: 5 247 1242 3726 1020
Fragments arranged by size:
3726 1242 1020 247 5
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 1897 2005 2218 2632 3238 3405 3412
Size: 331 1566 108 213 414 606 167 7
Cuts at: 3412 3808 3925 4002 4051 4165 4174 4279 4284
Size: 396 117 77 49 114 9 105 5
Cuts at: 4284 4729 4807 5029 5211 5644 5794 6240
Size: 445 78 222 182 433 150 446
Fragments arranged by size:
1566 606 446 445 433 414 396 331
222 213 182 167 150 117 114 108
105 78 77 49 9 7 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 847 1181 1246 1606 2020 2527 3208 5776
Size: 847 334 65 360 414 507 681 2568
Cuts at: 5776 6240
Size: 464
Fragments arranged by size:
2568 847 681 507 464 414 360 334
65
EcoO109I rG'GnC_Cy
Cuts at: 0 321 416 840 1058 1125 1239 1403 1750
Size: 321 95 424 218 67 114 164 347
Cuts at: 1750 1926 1989 2001 2178 2380 2985 3213 4080
Size: 176 63 12 177 202 605 228 867
Cuts at: 4080 4812 4900 4930 5160 5235 5637 5892 6240
Size: 732 88 30 230 75 402 255 348
Fragments arranged by size:
867 732 605 424 402 348 347 321
255 230 228 218 202 177 176 164
114 95 88 75 67 63 30 12
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3534 5274 6240
Size: 3534 1740 966
Fragments arranged by size:
3534 1740 966
FseI GG_CCGG'CC
Cuts at: 0 3657 4665 6240
Size: 3657 1008 1575
Fragments arranged by size:
3657 1575 1008
FspI TGC'GCA
Cuts at: 0 707 6240
Size: 707 5533
HaeII r_GCGC'y
Cuts at: 0 1173 1840 2342 2474 3225 3364 3684 3810
Size: 1173 667 502 132 751 139 320 126
Cuts at: 3810 4250 4458 5964 6240
Size: 440 208 1506 276
Fragments arranged by size:
1506 1173 751 667 502 440 320 276
208 139 132 126
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1114 1146 1702 1726 1729 1734 1758 1761
Size: 1114 32 556 24 3 5 24 3
Cuts at: 1761 4045 4077 4615 4647 5894 5926 6240
Size: 2284 32 538 32 1247 32 314
Fragments arranged by size:
2284 1247 1114 556 538 314 32 32
32 32 24 24 5 3 3
HincII GTy'rAC
Cuts at: 0 4398 5370 6240
Size: 4398 972 870
Fragments arranged by size:
4398 972 870
KasI G'GCGC_C
Cuts at: 0 1836 2338 3680 4246 6240
Size: 1836 502 1342 566 1994
Fragments arranged by size:
1994 1836 1342 566 502
KpnI G_GTAC'C
Cuts at: 0 1680 3327 3985 4098 5535 6240
Size: 1680 1647 658 113 1437 705
Fragments arranged by size:
1680 1647 1437 705 658 113
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2204 2825 4982 5159 6240
Size: 2204 621 2157 177 1081
Fragments arranged by size:
2204 2157 1081 621 177
MscI TGG'CCA
Cuts at: 0 412 571 2419 2569 2606 3896 5386 5702
Size: 412 159 1848 150 37 1290 1490 316
Cuts at: 5702 6240
Size: 538
Fragments arranged by size:
1848 1490 1290 538 412 316 159 150
37
MslI CAynn'nnrTG
Cuts at: 0 673 2245 2413 2699 2863 4199 4513 6240
Size: 673 1572 168 286 164 1336 314 1727
Fragments arranged by size:
1727 1572 1336 673 314 286 168 164
MspA1I CmG'CkG
Cuts at: 0 68 120 333 524 752 1017 1204 1432
Size: 68 52 213 191 228 265 187 228
Cuts at: 1432 1731 2130 2610 2658 3239 3345 3456 3548
Size: 299 399 480 48 581 106 111 92
Cuts at: 3548 3624 3783 3858 3882 4239 4304 4638 5466
Size: 76 159 75 24 357 65 334 828
Cuts at: 5466 5811 6015 6240
Size: 345 204 225
Fragments arranged by size:
828 581 480 399 357 345 334 299
265 228 228 225 213 204 191 187
159 111 106 92 76 75 68 65
52 48 24
NaeI GCC'GGC
Cuts at: 0 2029 2080 2286 2516 2706 2720 2785 3655
Size: 2029 51 206 230 190 14 65 870
Cuts at: 3655 4663 6201 6240
Size: 1008 1538 39
Fragments arranged by size:
2029 1538 1008 870 230 206 190 65
51 39 14
NarI GG'CG_CC
Cuts at: 0 1837 2339 3681 4247 6240
Size: 1837 502 1342 566 1993
Fragments arranged by size:
1993 1837 1342 566 502
NcoI C'CATG_G
Cuts at: 0 674 1133 1274 2588 3369 6240
Size: 674 459 141 1314 781 2871
Fragments arranged by size:
2871 1314 781 674 459 141
NdeI CA'TA_TG
Cuts at: 0 5549 6240
Size: 5549 691
NgoMIV G'CCGG_C
Cuts at: 0 2027 2078 2284 2514 2704 2718 2783 3653
Size: 2027 51 206 230 190 14 65 870
Cuts at: 3653 4661 6199 6240
Size: 1008 1538 41
Fragments arranged by size:
2027 1538 1008 870 230 206 190 65
51 41 14
NsiI A_TGCA'T
Cuts at: 0 5576 6240
Size: 5576 664
NspI r_CATG'y
Cuts at: 0 384 672 3174 5574 6240
Size: 384 288 2502 2400 666
Fragments arranged by size:
2502 2400 666 384 288
PasI CC'CwG_GG
Cuts at: 0 943 2334 2432 2838 5817 6240
Size: 943 1391 98 406 2979 423
Fragments arranged by size:
2979 1391 943 423 406 98
PciI A'CATG_T
Cuts at: 0 668 6240
Size: 668 5572
PflMI CCAn_nnn'nTGG
Cuts at: 0 309 755 1020 2168 2862 3504 3645 3727
Size: 309 446 265 1148 694 642 141 82
Cuts at: 3727 3903 4509 4767 6029 6240
Size: 176 606 258 1262 211
Fragments arranged by size:
1262 1148 694 642 606 446 309 265
258 211 176 141 82
PfoI T'CCnGG_A
Cuts at: 0 41 2141 3833 3902 4706 5345 6161 6240
Size: 41 2100 1692 69 804 639 816 79
Fragments arranged by size:
2100 1692 816 804 639 79 69 41
PmlI CAC'GTG
Cuts at: 0 645 1922 3299 4038 5706 6240
Size: 645 1277 1377 739 1668 534
Fragments arranged by size:
1668 1377 1277 739 645 534
PpuMI rG'GwC_Cy
Cuts at: 0 840 1239 1926 1989 2001 2380 4930 5235
Size: 840 399 687 63 12 379 2550 305
Cuts at: 5235 6240
Size: 1005
Fragments arranged by size:
2550 1005 840 687 399 379 305 63
12
PshAI GACnn'nnGTC
Cuts at: 0 2378 6240
Size: 2378 3862
PspOMI G'GGCC_C
Cuts at: 0 416 1749 4079 4366 4812 6092 6240
Size: 416 1333 2330 287 446 1280 148
Fragments arranged by size:
2330 1333 1280 446 416 287 148
PspXI vC'TCGA_Gb
Cuts at: 0 4273 4327 6240
Size: 4273 54 1913
Fragments arranged by size:
4273 1913 54
PstI C_TGCA'G
Cuts at: 0 149 1431 1607 2036 2255 3195 3284 3623
Size: 149 1282 176 429 219 940 89 339
Cuts at: 3623 3806 3944 3968 4244 4687 5210 6220 6240
Size: 183 138 24 276 443 523 1010 20
Fragments arranged by size:
1282 1010 940 523 443 429 339 276
219 183 176 149 138 89 24 20
PvuI CG_AT'CG
Cuts at: 0 4838 6240
Size: 4838 1402
PvuII CAG'CTG
Cuts at: 0 752 1017 2610 2658 3624 3783 4239 4304
Size: 752 265 1593 48 966 159 456 65
Cuts at: 4304 5466 5811 6240
Size: 1162 345 429
Fragments arranged by size:
1593 1162 966 752 456 429 345 265
159 65 48
RsrII CG'GwC_CG
Cuts at: 0 111 2798 6240
Size: 111 2687 3442
Fragments arranged by size:
3442 2687 111
SacI G_AGCT'C
Cuts at: 0 7 254 1496 5222 6240
Size: 7 247 1242 3726 1018
Fragments arranged by size:
3726 1242 1018 247 7
SanDI GG'GwC_CC
Cuts at: 0 1926 2380 4930 6240
Size: 1926 454 2550 1310
Fragments arranged by size:
2550 1926 1310 454
SapI GCTCTTCn'nnn_
Cuts at: 0 2089 2608 3568 5086 5227 6240
Size: 2089 519 960 1518 141 1013
Fragments arranged by size:
2089 1518 1013 960 519 141
SbfI CC_TGCA'GG
Cuts at: 0 3195 3944 6240
Size: 3195 749 2296
Fragments arranged by size:
3195 2296 749
ScaI AGT'ACT
Cuts at: 0 151 6240
Size: 151 6089
SexAI A'CCwGG_T
Cuts at: 0 4818 5237 6240
Size: 4818 419 1003
Fragments arranged by size:
4818 1003 419
SfcI C'TryA_G
Cuts at: 0 145 1427 1603 2010 2032 2251 3191 3280
Size: 145 1282 176 407 22 219 940 89
Cuts at: 3280 3619 3802 3940 3964 4240 4683 5206 6216
Size: 339 183 138 24 276 443 523 1010
Cuts at: 6216 6240
Size: 24
Fragments arranged by size:
1282 1010 940 523 443 407 339 276
219 183 176 145 138 89 24 24
22
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 424 3659 6240
Size: 424 3235 2581
Fragments arranged by size:
3235 2581 424
SfoI GGC'GCC
Cuts at: 0 1838 2340 3682 4248 6240
Size: 1838 502 1342 566 1992
Fragments arranged by size:
1992 1838 1342 566 502
SgrAI Cr'CCGG_yG
Cuts at: 0 4156 6240
Size: 4156 2084
SmaI CCC'GGG
Cuts at: 0 421 1401 2699 3247 3916 4365 5101 6240
Size: 421 980 1298 548 669 449 736 1139
Fragments arranged by size:
1298 1139 980 736 669 548 449 421
SmlI C'TyrA_G
Cuts at: 0 2047 2208 2980 3490 4273 4327 6034 6240
Size: 2047 161 772 510 783 54 1707 206
Fragments arranged by size:
2047 1707 783 772 510 206 161 54
SphI G_CATG'C
Cuts at: 0 5574 6240
Size: 5574 666
SrfI GCCC'GGGC
Cuts at: 0 421 6240
Size: 421 5819
StuI AGG'CCT
Cuts at: 0 733 3418 5191 5875 6240
Size: 733 2685 1773 684 365
Fragments arranged by size:
2685 1773 733 684 365
StyI C'CwwG_G
Cuts at: 0 572 674 843 1133 1274 2243 2588 3369
Size: 572 102 169 290 141 969 345 781
Cuts at: 3369 5762 6240
Size: 2393 478
Fragments arranged by size:
2393 969 781 572 478 345 290 169
141 102
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 178 3142 3191 4407 6240
Size: 178 2964 49 1216 1833
Fragments arranged by size:
2964 1833 1216 178 49
TatI w'GTAC_w
Cuts at: 0 104 149 2201 2873 4943 5024 6240
Size: 104 45 2052 672 2070 81 1216
Fragments arranged by size:
2070 2052 1216 672 104 81 45
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1364 1396 3876 3908 6240
Size: 1364 32 2480 32 2332
Fragments arranged by size:
2480 2332 1364 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 136 1582 3154 4180 5239 6240
Size: 136 1446 1572 1026 1059 1001
Fragments arranged by size:
1572 1446 1059 1026 1001 136
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1971 2269 2391 4653 4704 4764 4965 5235
Size: 1971 298 122 2262 51 60 201 270
Cuts at: 5235 5769 6003 6240
Size: 534 234 237
Fragments arranged by size:
2262 1971 534 298 270 237 234 201
122 60 51
XhoI C'TCGA_G
Cuts at: 0 4273 4327 6240
Size: 4273 54 1913
Fragments arranged by size:
4273 1913 54
XmaI C'CCGG_G
Cuts at: 0 419 1399 2697 3245 3914 4363 5099 6240
Size: 419 980 1298 548 669 449 736 1141
Fragments arranged by size:
1298 1141 980 736 669 548 449 419
XmnI GAAnn'nnTTC
Cuts at: 0 4795 6240
Size: 4795 1445
ZraI GAC'GTC
Cuts at: 0 2535 6240
Size: 2535 3705
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI AvaI AvrII BamHI
BanI BanII BbeI BbsI BbvCI BcgI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI
BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FalI FseI FspI HaeII Hin4I HincII KasI
KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NsiI NspI PasI PciI PflMI PfoI
PmlI PpuMI PshAI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgrAI SmaI SmlI SphI SrfI StuI
StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AclI AflII AhdI AloI ApoI AscI AseI AsiSI
BaeI BciVI BmtI BsaBI BsiWI BsmI BspHI BsrDI
BstBI CspCI DraI EcoRI EcoRV FspAI HindIII HpaI
MfeI MluI NheI NotI NruI PacI PmeI PpiI
PsiI PsrI SacII SalI SgfI SnaBI SpeI SspI
SwaI XbaI