(Circular) (Six-base) MAPSORT of: pFN21ASDA1710.seq Check: 2027 from: 1 to: 6139 pFN21ASDA1710 6139 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3949 278 Size: 53 83 186 936 2413 2468 Fragments arranged by size: 2468 2413 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3482 1937 Size: 1545 4594 Acc65I G'GTAC_C Cuts at: 3460 3460 Size: 6139 AclI AA'CG_TT Cuts at: 1563 4266 4639 1563 Size: 2703 373 3063 Fragments arranged by size: 3063 2703 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2446 2530 2782 2866 2950 3118 4202 5586 Size: 814 84 252 84 84 168 1084 1384 Cuts at: 5586 5854 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1084 814 268 252 168 84 84 84 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5942 AflIII A'CryG_T Cuts at: 1203 5038 6015 1203 Size: 3835 977 1327 Fragments arranged by size: 3835 1327 977 AgeI A'CCGG_T Cuts at: 4970 4970 Size: 6139 AhdI GACnn_n'nnGTC Cuts at: 4868 4868 Size: 6139 AleI CACnn'nnGTG Cuts at: 1166 2757 2841 1166 Size: 1591 84 4464 Fragments arranged by size: 4464 1591 84 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6139 AlwNI CAG_nnn'CTG Cuts at: 2390 3882 5454 2390 Size: 1492 1572 3075 Fragments arranged by size: 3075 1572 1492 ApaLI G'TGCA_C Cuts at: 4196 5352 4196 Size: 1156 4983 ApoI r'AATT_y Cuts at: 1493 3046 3168 3448 3674 3826 5935 1493 Size: 1553 122 280 226 152 2109 1697 Fragments arranged by size: 2109 1697 1553 280 226 152 122 AseI AT'TA_AT Cuts at: 160 1032 4693 160 Size: 872 3661 1606 Fragments arranged by size: 3661 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6139 AvaI C'yCGr_G Cuts at: 1870 1943 2094 3464 1870 Size: 73 151 1370 4545 Fragments arranged by size: 4545 1370 151 73 BamHI G'GATC_C Cuts at: 3469 3836 3469 Size: 367 5772 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2056 3460 4915 618 Size: 325 217 627 269 1404 1455 1842 Fragments arranged by size: 1842 1455 1404 627 325 269 217 BanII G_rGCy'C Cuts at: 1391 2810 3004 3146 3314 3398 3458 1391 Size: 1419 194 142 168 84 60 4072 Fragments arranged by size: 4072 1419 194 168 142 84 60 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2501 928 Size: 971 602 4566 Fragments arranged by size: 4566 971 602 BbvCI CC'TCA_GC Cuts at: 2280 2280 Size: 6139 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4330 4364 1903 Size: 34 2393 34 3678 Fragments arranged by size: 3678 2393 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4031 5241 4031 Size: 1210 4929 BclI T'GATC_A Cuts at: 1548 1548 Size: 6139 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4750 136 Size: 107 122 71 631 605 3078 1525 Fragments arranged by size: 3078 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2469 2891 3232 6134 2469 Size: 422 341 2902 2474 Fragments arranged by size: 2902 2474 422 341 BlpI GC'TnA_GC Cuts at: 2820 3397 3556 2820 Size: 577 159 5403 Fragments arranged by size: 5403 577 159 Bme1580I G_kGCm'C Cuts at: 1790 4200 5356 1790 Size: 2410 1156 2573 Fragments arranged by size: 2573 2410 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6139 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2598 3870 4828 Size: 416 53 83 399 24 1181 1272 958 Cuts at: 4828 442 Size: 1753 Fragments arranged by size: 1753 1272 1181 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6139 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3417 3417 Size: 6139 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2526 2694 3114 3198 4799 1239 Size: 1287 168 420 84 1601 2579 Fragments arranged by size: 2579 1601 1287 420 168 84 Bpu10I CC'TnA_GC Cuts at: 2280 2280 Size: 6139 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4264 5129 5427 4264 Size: 865 298 4976 Fragments arranged by size: 4976 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2101 4802 882 Size: 617 602 2701 2219 Fragments arranged by size: 2701 2219 617 602 BsaAI yAC'GTr Cuts at: 493 1204 3436 3930 493 Size: 711 2232 494 2702 Fragments arranged by size: 2702 2232 711 494 BsaBI GATnn'nnATC Cuts at: 6133 6133 Size: 6139 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3946 4328 4963 275 Size: 53 83 186 936 2413 382 635 1451 Fragments arranged by size: 2413 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4572 4970 5244 5391 5802 4572 Size: 398 274 147 411 4909 Fragments arranged by size: 4909 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5914 Fragments arranged by size: 5914 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2795 1186 Size: 1609 4530 BseYI C'CCAG_C Cuts at: 2045 5342 5838 2045 Size: 3297 496 2346 Fragments arranged by size: 3297 2346 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2106 3305 1163 Size: 573 370 1199 3997 Fragments arranged by size: 3997 1199 573 370 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3589 4350 5378 1540 Size: 275 186 1588 761 1028 2301 Fragments arranged by size: 2301 1588 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2810 3146 3314 3398 4200 4285 5356 2810 Size: 336 168 84 802 85 1071 3593 Fragments arranged by size: 3593 1071 802 336 168 85 84 BsmI GAATG_Cn' Cuts at: 2281 3116 3650 3743 2281 Size: 835 534 93 4677 Fragments arranged by size: 4677 835 534 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2726 2726 Size: 6139 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2810 3004 3146 3314 3398 3458 4200 Size: 399 1020 194 142 168 84 60 742 Cuts at: 4200 4285 5356 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 1020 742 399 194 168 142 85 84 60 BspEI T'CCGG_A Cuts at: 5802 5802 Size: 6139 BspHI T'CATG_A Cuts at: 4028 5758 4028 Size: 1730 4409 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2275 3494 844 Size: 1431 1219 3489 Fragments arranged by size: 3489 1431 1219 BsrBI CCG'CTC Cuts at: 1394 1823 4026 1394 Size: 429 2203 3507 Fragments arranged by size: 3507 2203 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3121 4634 4808 65 Size: 1183 1873 1513 174 1396 Fragments arranged by size: 1873 1513 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4783 4970 1700 Size: 110 2973 187 2869 Fragments arranged by size: 2973 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2239 96 Size: 2143 3996 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6139 BssSI C'ACGA_G Cuts at: 4199 5211 4199 Size: 1012 5127 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6139 BstBI TT'CG_AA Cuts at: 2007 4943 2007 Size: 2936 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6139 BstYI r'GATC_y Cuts at: 1972 2469 2891 3232 3469 3836 4221 4238 5690 Size: 497 422 341 237 367 385 17 1452 Cuts at: 5690 5776 5788 6134 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 497 422 385 367 346 341 237 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 513 Size: 552 957 4630 Fragments arranged by size: 4630 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2978 487 Size: 683 56 1752 3648 Fragments arranged by size: 3648 1752 683 56 BtsI GCAGTG_nn' Cuts at: 3293 3655 4449 4469 3293 Size: 362 794 20 4963 Fragments arranged by size: 4963 794 362 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6139 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3103 3138 529 Size: 35 2539 35 3530 Fragments arranged by size: 3530 2539 35 35 DraI TTT'AAA Cuts at: 3443 3443 Size: 6139 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5146 809 Size: 507 3830 1802 Fragments arranged by size: 3830 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2079 2872 3586 Size: 54 1425 50 275 18 249 793 714 Cuts at: 3586 4475 6104 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 793 714 275 249 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3586 1537 Size: 275 1774 4090 Fragments arranged by size: 4090 1774 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2295 2352 3374 4069 1353 Size: 942 57 1022 695 3423 Fragments arranged by size: 3423 1022 942 695 57 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4726 5100 5246 387 Size: 1064 138 3137 374 146 1280 Fragments arranged by size: 3137 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2808 3144 3312 3396 2808 Size: 336 168 84 5551 Fragments arranged by size: 5551 336 168 84 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2446 2526 2530 2694 2782 2866 2950 Size: 393 814 80 4 164 88 84 84 Cuts at: 2950 3114 3118 3198 4202 4799 5586 5854 1239 Size: 164 4 80 1004 597 787 268 1524 Fragments arranged by size: 1524 1004 814 787 597 393 268 164 164 88 84 84 80 80 4 4 EcoO109I rG'GnC_Cy Cuts at: 2049 2131 2748 2832 2934 2049 Size: 82 617 84 102 5254 Fragments arranged by size: 5254 617 102 84 82 EcoRI G'AATT_C Cuts at: 3448 3826 3448 Size: 378 5761 FspI TGC'GCA Cuts at: 4645 4645 Size: 6139 HaeII r_GCGC'y Cuts at: 1678 5286 1678 Size: 3608 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2641 2673 2881 2883 2913 Size: 32 135 32 1069 32 208 2 30 Cuts at: 2913 2915 3133 3165 4780 4812 4854 4886 1373 Size: 2 218 32 1615 32 42 32 2626 Fragments arranged by size: 2626 1615 1069 218 208 135 42 32 32 32 32 32 32 30 2 2 HincII GTy'rAC Cuts at: 669 1657 1938 3483 3729 669 Size: 988 281 1545 246 3079 Fragments arranged by size: 3079 1545 988 281 246 HindIII A'AGCT_T Cuts at: 748 2794 3130 3214 748 Size: 2046 336 84 3673 Fragments arranged by size: 3673 2046 336 84 HpaI GTT'AAC Cuts at: 3729 3729 Size: 6139 KpnI G_GTAC'C Cuts at: 3464 3464 Size: 6139 MfeI C'AATT_G Cuts at: 3738 3738 Size: 6139 MluI A'CGCG_T Cuts at: 6015 6015 Size: 6139 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2404 2761 3003 3339 5253 5437 1305 Size: 1099 357 242 336 1914 184 2007 Fragments arranged by size: 2007 1914 1099 357 336 242 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2081 10 Size: 54 1425 343 249 4068 Fragments arranged by size: 4068 1425 343 249 54 MslI CAynn'nnrTG Cuts at: 518 1166 2757 2841 3109 4097 4456 4615 518 Size: 648 1591 84 268 988 359 159 2042 Fragments arranged by size: 2042 1591 988 648 359 268 159 84 MspA1I CmG'CkG Cuts at: 1606 3555 4232 5380 5625 1606 Size: 1949 677 1148 245 2120 Fragments arranged by size: 2120 1949 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4630 Fragments arranged by size: 4630 957 552 NdeI CA'TA_TG Cuts at: 387 3106 387 Size: 2719 3420 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6139 NotI GC'GGCC_GC Cuts at: 3586 3586 Size: 6139 NspI r_CATG'y Cuts at: 1132 1229 3497 5042 1132 Size: 97 2268 1545 2229 Fragments arranged by size: 2268 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6139 PciI A'CATG_T Cuts at: 5038 5038 Size: 6139 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6139 PmeI GTTT'AAAC Cuts at: 3443 3443 Size: 6139 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4208 4240 5721 5753 4208 Size: 32 1481 32 4594 Fragments arranged by size: 4594 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2049 2131 2748 2832 2049 Size: 82 617 84 5356 Fragments arranged by size: 5356 617 84 82 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6139 PsiI TTA'TAA Cuts at: 3709 3709 Size: 6139 PstI C_TGCA'G Cuts at: 830 3491 830 Size: 2661 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6139 SacI G_AGCT'C Cuts at: 2810 3146 3314 3398 2810 Size: 336 168 84 5551 Fragments arranged by size: 5551 336 168 84 SalI G'TCGA_C Cuts at: 1936 3481 1936 Size: 1545 4594 SanDI GG'GwC_CC Cuts at: 2049 2049 Size: 6139 SbfI CC_TGCA'GG Cuts at: 3491 3491 Size: 6139 ScaI AGT'ACT Cuts at: 4387 6099 4387 Size: 1712 4427 SfcI C'TryA_G Cuts at: 826 1045 3487 4622 5303 5494 826 Size: 219 2442 1135 681 191 1471 Fragments arranged by size: 2442 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6139 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6139 SmaI CCC'GGG Cuts at: 1872 2096 3466 1872 Size: 224 1370 4545 Fragments arranged by size: 4545 1370 224 SmlI C'TyrA_G Cuts at: 820 1017 1943 4243 5144 5406 820 Size: 197 926 2300 901 262 1553 Fragments arranged by size: 2300 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3436 3930 493 Size: 2943 494 2702 Fragments arranged by size: 2943 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6139 SphI G_CATG'C Cuts at: 1132 3497 1132 Size: 2365 3774 SspI AAT'ATT Cuts at: 5 52 4063 5 Size: 47 4011 2081 Fragments arranged by size: 4011 2081 47 StuI AGG'CCT Cuts at: 1517 2459 2599 1517 Size: 942 140 5057 Fragments arranged by size: 5057 942 140 StyI C'CwwG_G Cuts at: 513 1065 2022 3578 513 Size: 552 957 1556 3074 Fragments arranged by size: 3074 1556 957 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3320 4166 4183 4336 4521 4966 325 Size: 1255 1740 846 17 153 185 445 1498 Fragments arranged by size: 1740 1498 1255 846 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2239 4385 6097 Size: 274 80 33 51 1443 262 2146 1712 Cuts at: 6097 96 Size: 138 Fragments arranged by size: 2146 1712 1443 274 262 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2740 2772 6099 6131 2740 Size: 32 3327 32 2748 Fragments arranged by size: 3327 2748 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6139 XbaI T'CTAG_A Cuts at: 3475 3475 Size: 6139 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2038 2038 Size: 6139 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6139 XmaI C'CCGG_G Cuts at: 1870 2094 3464 1870 Size: 224 1370 4545 Fragments arranged by size: 4545 1370 224 XmnI GAAnn'nnTTC Cuts at: 2638 2795 2963 3131 3299 3383 4268 2638 Size: 157 168 168 168 84 885 4509 Fragments arranged by size: 4509 885 168 168 168 157 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3947 276 Size: 53 83 186 936 2413 2468 Fragments arranged by size: 2468 2413 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI