(Circular) (Six-base) MAPSORT of: pFN21ASDA1379.seq Check: 4768 from: 1 to: 6061 pFN21ASDA1379 6061 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2698 2698 Size: 6061 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3871 278 Size: 53 83 186 936 2335 2468 Fragments arranged by size: 2468 2335 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3404 1937 Size: 1467 4594 Acc65I G'GTAC_C Cuts at: 3382 3382 Size: 6061 AclI AA'CG_TT Cuts at: 1563 4188 4561 1563 Size: 2625 373 3063 Fragments arranged by size: 3063 2625 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2448 4124 5508 5776 1632 Size: 816 1676 1384 268 1917 Fragments arranged by size: 1917 1676 1384 816 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5864 AflIII A'CryG_T Cuts at: 1203 4960 5937 1203 Size: 3757 977 1327 Fragments arranged by size: 3757 1327 977 AgeI A'CCGG_T Cuts at: 4892 4892 Size: 6061 AhdI GACnn_n'nnGTC Cuts at: 4790 4790 Size: 6061 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6061 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6061 AlwNI CAG_nnn'CTG Cuts at: 2079 3804 5376 2079 Size: 1725 1572 2764 Fragments arranged by size: 2764 1725 1572 ApaLI G'TGCA_C Cuts at: 4118 5274 4118 Size: 1156 4905 ApoI r'AATT_y Cuts at: 1493 3370 3596 3748 5857 1493 Size: 1877 226 152 2109 1697 Fragments arranged by size: 2109 1877 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4615 160 Size: 872 3583 1606 Fragments arranged by size: 3583 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6061 AvaI C'yCGr_G Cuts at: 1870 1943 3386 1870 Size: 73 1443 4545 Fragments arranged by size: 4545 1443 73 BamHI G'GATC_C Cuts at: 3391 3758 3391 Size: 367 5694 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2053 2223 2276 2784 3152 Size: 325 217 627 266 170 53 508 368 Cuts at: 3152 3291 3382 4837 618 Size: 139 91 1455 1842 Fragments arranged by size: 1842 1455 627 508 368 325 266 217 170 139 91 53 BanII G_rGCy'C Cuts at: 1391 3234 3380 1391 Size: 1843 146 4072 Fragments arranged by size: 4072 1843 146 BbeI G_GCGC'C Cuts at: 2057 2227 3156 2057 Size: 170 929 4962 Fragments arranged by size: 4962 929 170 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2639 2743 2874 928 Size: 971 740 104 131 4115 Fragments arranged by size: 4115 971 740 131 104 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4252 4286 1903 Size: 34 2315 34 3678 Fragments arranged by size: 3678 2315 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3953 5163 3953 Size: 1210 4851 BclI T'GATC_A Cuts at: 1548 1548 Size: 6061 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2051 2495 3020 Size: 107 122 71 631 605 379 444 525 Cuts at: 3020 4672 136 Size: 1652 1525 Fragments arranged by size: 1652 1525 631 605 525 444 379 122 107 71 BglII A'GATC_T Cuts at: 6056 6056 Size: 6061 BlpI GC'TnA_GC Cuts at: 3233 3478 3233 Size: 245 5816 Bme1580I G_kGCm'C Cuts at: 1790 3199 4122 5278 1790 Size: 1409 923 1156 2573 Fragments arranged by size: 2573 1409 1156 923 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6061 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2218 2680 2917 Size: 416 53 83 399 24 801 462 237 Cuts at: 2917 3027 3792 4750 442 Size: 110 765 958 1753 Fragments arranged by size: 1753 958 801 765 462 416 399 237 110 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6061 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3190 3272 3190 Size: 82 5979 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2282 2372 2501 2855 4721 1239 Size: 1043 90 129 354 1866 2579 Fragments arranged by size: 2579 1866 1043 354 129 90 Bpu10I CC'TnA_GC Cuts at: 2577 2577 Size: 6061 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2741 4186 5051 5349 2741 Size: 1445 865 298 3453 Fragments arranged by size: 3453 1445 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2057 3134 4724 882 Size: 617 558 1077 1590 2219 Fragments arranged by size: 2219 1590 1077 617 558 BsaAI yAC'GTr Cuts at: 493 1204 3343 3358 3852 493 Size: 711 2139 15 494 2702 Fragments arranged by size: 2702 2139 711 494 15 BsaBI GATnn'nnATC Cuts at: 6055 6055 Size: 6061 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2054 2224 2379 3153 Size: 53 83 186 936 521 170 155 774 Cuts at: 3153 3868 4250 4885 275 Size: 715 382 635 1451 Fragments arranged by size: 1451 936 774 715 635 521 382 186 170 155 83 53 BsaWI w'CCGG_w Cuts at: 4494 4892 5166 5313 5724 4494 Size: 398 274 147 411 4831 Fragments arranged by size: 4831 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2696 2726 3174 3204 1178 Size: 30 165 30 1293 30 448 30 4035 Fragments arranged by size: 4035 1293 448 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2075 2945 3287 1186 Size: 889 870 342 3960 Fragments arranged by size: 3960 889 870 342 BseYI C'CCAG_C Cuts at: 3044 3265 3319 5264 5760 3044 Size: 221 54 1945 496 3345 Fragments arranged by size: 3345 1945 496 221 54 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2180 2297 2648 1163 Size: 573 444 117 351 4576 Fragments arranged by size: 4576 573 444 351 117 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3511 4272 5300 1540 Size: 275 186 1510 761 1028 2301 Fragments arranged by size: 2301 1510 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3234 4122 4207 5278 3234 Size: 888 85 1071 4017 Fragments arranged by size: 4017 1071 888 85 BsiWI C'GTAC_G Cuts at: 2187 2187 Size: 6061 BsmI GAATG_Cn' Cuts at: 3572 3665 3572 Size: 93 5968 BsmBI CGTCTCn'nnnn_ Cuts at: 2408 3243 2408 Size: 835 5226 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3199 3234 3380 4122 4207 5278 1391 Size: 399 1409 35 146 742 85 1071 2174 Fragments arranged by size: 2174 1409 1071 742 399 146 85 35 BspEI T'CCGG_A Cuts at: 5724 5724 Size: 6061 BspHI T'CATG_A Cuts at: 3950 5680 3950 Size: 1730 4331 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2698 3416 844 Size: 1854 718 3489 Fragments arranged by size: 3489 1854 718 BsrBI CCG'CTC Cuts at: 1394 1823 2266 3948 1394 Size: 429 443 1682 3507 Fragments arranged by size: 3507 1682 443 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2726 4556 4730 65 Size: 1183 1478 1830 174 1396 Fragments arranged by size: 1830 1478 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4705 4892 1700 Size: 110 2895 187 2869 Fragments arranged by size: 2895 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6061 BssHII G'CGCG_C Cuts at: 1925 2167 1925 Size: 242 5819 BssSI C'ACGA_G Cuts at: 4121 5133 4121 Size: 1012 5049 BstAPI GCAn_nnn'nTGC Cuts at: 794 2626 794 Size: 1832 4229 BstBI TT'CG_AA Cuts at: 2007 4865 2007 Size: 2858 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6061 BstXI CCAn_nnnn'nTGG Cuts at: 3343 3343 Size: 6061 BstYI r'GATC_y Cuts at: 1972 3391 3758 4143 4160 5612 5698 5710 6056 Size: 1419 367 385 17 1452 86 12 346 Cuts at: 6056 1972 Size: 1977 Fragments arranged by size: 1977 1452 1419 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3052 3295 513 Size: 552 1987 243 3279 Fragments arranged by size: 3279 1987 552 243 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5322 Fragments arranged by size: 5322 683 56 BtsI GCAGTG_nn' Cuts at: 3064 3142 3577 4371 4391 3064 Size: 78 435 794 20 4734 Fragments arranged by size: 4734 794 435 78 20 ClaI AT'CG_AT Cuts at: 1554 2118 1554 Size: 564 5497 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6026 DraI TTT'AAA Cuts at: 3365 3365 Size: 6061 DraIII CAC_nnn'GTG Cuts at: 3099 3099 Size: 6061 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5068 809 Size: 507 3752 1802 Fragments arranged by size: 3752 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2125 2529 3214 Size: 54 1425 50 275 18 295 404 685 Cuts at: 3214 3508 4397 6026 8 Size: 294 889 1629 43 Fragments arranged by size: 1629 1425 889 685 404 295 294 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3508 1537 Size: 275 1696 4090 Fragments arranged by size: 4090 1696 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3991 1353 Size: 2638 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4648 5022 5168 387 Size: 1064 138 3059 374 146 1280 Fragments arranged by size: 3059 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3232 3232 Size: 6061 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2282 2372 2448 2501 2855 4124 4721 Size: 393 650 90 76 53 354 1269 597 Cuts at: 4721 5508 5776 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1269 787 650 597 393 354 268 90 76 53 EcoNI CCTnn'n_nnAGG Cuts at: 2354 2759 2354 Size: 405 5656 EcoO109I rG'GnC_Cy Cuts at: 2347 2347 Size: 6061 EcoRI G'AATT_C Cuts at: 3370 3748 3370 Size: 378 5683 FspI TGC'GCA Cuts at: 4567 4567 Size: 6061 HaeII r_GCGC'y Cuts at: 1678 2057 2227 3005 3156 5208 1678 Size: 379 170 778 151 2052 2531 Fragments arranged by size: 2531 2052 778 379 170 151 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2030 2062 3020 3052 4702 Size: 32 135 32 458 32 958 32 1650 Cuts at: 4702 4734 4776 4808 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1650 958 458 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3405 3651 669 Size: 988 281 1467 246 3079 Fragments arranged by size: 3079 1467 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6061 HpaI GTT'AAC Cuts at: 3651 3651 Size: 6061 KasI G'GCGC_C Cuts at: 2053 2223 3152 2053 Size: 170 929 4962 Fragments arranged by size: 4962 929 170 KpnI G_GTAC'C Cuts at: 3386 3386 Size: 6061 MfeI C'AATT_G Cuts at: 3660 3660 Size: 6061 MluI A'CGCG_T Cuts at: 5937 5937 Size: 6061 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5175 5359 1305 Size: 3870 184 2007 Fragments arranged by size: 3870 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2531 10 Size: 54 1425 343 699 3540 Fragments arranged by size: 3540 1425 699 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2819 2909 4019 4378 4537 518 Size: 648 1653 90 1110 359 159 2042 Fragments arranged by size: 2042 1653 1110 648 359 159 90 MspA1I CmG'CkG Cuts at: 1606 3054 3312 3319 3477 4154 5302 5547 1606 Size: 1448 258 7 158 677 1148 245 2120 Fragments arranged by size: 2120 1448 1148 677 258 245 158 7 NarI GG'CG_CC Cuts at: 2054 2224 3153 2054 Size: 170 929 4962 Fragments arranged by size: 4962 929 170 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5509 NdeI CA'TA_TG Cuts at: 387 387 Size: 6061 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6061 NotI GC'GGCC_GC Cuts at: 3508 3508 Size: 6061 NspI r_CATG'y Cuts at: 1132 1229 2908 3419 4964 1132 Size: 97 1679 511 1545 2229 Fragments arranged by size: 2229 1679 1545 511 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6061 PciI A'CATG_T Cuts at: 4960 4960 Size: 6061 PflMI CCAn_nnn'nTGG Cuts at: 1108 2220 2252 2692 2916 3099 1108 Size: 1112 32 440 224 183 4070 Fragments arranged by size: 4070 1112 440 224 183 32 PmeI GTTT'AAAC Cuts at: 3365 3365 Size: 6061 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4130 4162 5643 5675 4130 Size: 32 1481 32 4516 Fragments arranged by size: 4516 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2347 2347 Size: 6061 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6061 PsiI TTA'TAA Cuts at: 3631 3631 Size: 6061 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2795 2827 2795 Size: 32 6029 PstI C_TGCA'G Cuts at: 830 2598 3413 830 Size: 1768 815 3478 Fragments arranged by size: 3478 1768 815 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6061 PvuII CAG'CTG Cuts at: 3319 3319 Size: 6061 RsrII CG'GwC_CG Cuts at: 2103 2103 Size: 6061 SacI G_AGCT'C Cuts at: 3234 3234 Size: 6061 SacII CC_GC'GG Cuts at: 3055 3055 Size: 6061 SalI G'TCGA_C Cuts at: 1936 3403 1936 Size: 1467 4594 SbfI CC_TGCA'GG Cuts at: 3413 3413 Size: 6061 ScaI AGT'ACT Cuts at: 4309 6021 4309 Size: 1712 4349 SfcI C'TryA_G Cuts at: 826 1045 2594 3409 4544 5225 5416 826 Size: 219 1549 815 1135 681 191 1471 Fragments arranged by size: 1549 1471 1135 815 681 219 191 SfoI GGC'GCC Cuts at: 2055 2225 3154 2055 Size: 170 929 4962 Fragments arranged by size: 4962 929 170 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6061 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6061 SmaI CCC'GGG Cuts at: 1872 3388 1872 Size: 1516 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2756 4165 5066 5328 820 Size: 197 926 813 1409 901 262 1553 Fragments arranged by size: 1553 1409 926 901 813 262 197 SnaBI TAC'GTA Cuts at: 493 3358 3852 493 Size: 2865 494 2702 Fragments arranged by size: 2865 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6061 SphI G_CATG'C Cuts at: 1132 2908 3419 1132 Size: 1776 511 3774 Fragments arranged by size: 3774 1776 511 SspI AAT'ATT Cuts at: 5 52 3985 5 Size: 47 3933 2081 Fragments arranged by size: 3933 2081 47 StuI AGG'CCT Cuts at: 1517 2045 2498 1517 Size: 528 453 5080 Fragments arranged by size: 5080 528 453 StyI C'CwwG_G Cuts at: 513 1065 2456 3177 3500 513 Size: 552 1391 721 323 3074 Fragments arranged by size: 3074 1391 721 552 323 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2390 2557 4088 4105 4258 4443 4888 Size: 1255 810 167 1531 17 153 185 445 Cuts at: 4888 325 Size: 1498 Fragments arranged by size: 1531 1498 1255 810 445 185 167 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2688 4307 6019 Size: 274 80 33 51 1443 711 1619 1712 Cuts at: 6019 96 Size: 138 Fragments arranged by size: 1712 1619 1443 711 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3174 3206 6021 6053 3174 Size: 32 2815 32 3182 Fragments arranged by size: 3182 2815 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6061 XbaI T'CTAG_A Cuts at: 3397 3397 Size: 6061 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6061 XmaI C'CCGG_G Cuts at: 1870 3386 1870 Size: 1516 4545 XmnI GAAnn'nnTTC Cuts at: 4190 4190 Size: 6061 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3869 276 Size: 53 83 186 936 2335 2468 Fragments arranged by size: 2468 2335 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BstZ17I Bsu36I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI SanDI SapI SexAI SfiI SrfI SwaI XcmI