Restriction map for
pFN21ASDA1073
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AflIII AgeI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AviII AvrII BanI BbeI BbrPI
BbvCI BclI BglII BlnI BsaAI
BsaI BsaWI BseAI BseRI BsiWI
BsmFI BsmI Bsp1407I BspEI BspHI
BspLU11I BsrBI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I CpoI DraI
DrdI EarI Eco47III EcoNI EcoO65I
FauI FbaI FseI FspI HgaI
HincII HindII HpaI KasI KpnI
Ksp632I KspI MfeI MluI MroI
MunI MvnI NarI NdeI NheI
NotI NruI NspV PacI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI Sse8387I SspBI StuI
SwaI XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AatII 1230 GACGTC GACGT^C
AcyI 1230 GACGTC G[GA]^CG[CT]C
AflII 1096 CTTAAG C^TTAAG
AhdI 67 GACTCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 122 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 1771 GAACCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 67 GACTCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 12 CTCGAG C^[CT]CG[GA]G
BamHI 357 GGATCC G^GATCC
BcgI 1420 GCAGACAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 962 CCCGG CC^[GC]GG
BfrI 1096 CTTAAG C^TTAAG
BglI 985 GCCAACAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1273 GCTCAGC GC^T[ACGT]AGC
BpmI 672 CTGGAG CTGGAG(16/14)
Bpu1102I 1273 GCTCAGC GC^T[ACGT]AGC
BsaBI 1021 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1230 GACGTC G[GA]^CG[CT]C
BsgI 1895 CTGCAC GTGCAG(16/14)
BsiEI 52 CGGCCG CG[GA][CT]^CG
BsmBI 4 GAGACG CGTCTC(1/5)
BsoBI 12 CTCGAG C^[CT]CG[GA]G
BspDI 1373 ATCGAT AT^CGAT
BspMI 566 ACCTGC ACCTGC(4/8)
BsrDI 1246 CATTGC GCAATG(2/0)
BsrFI 39 GCCGGC [GA]^CCGG[CT]
CelII 1273 GCTCAGC GC^T[ACGT]AGC
Cfr10I 39 GCCGGC [GA]^CCGG[CT]
ClaI 1373 ATCGAT AT^CGAT
DraII 185 GGGTCCT [GA]G^G[ACGT]CC[CT]
EagI 52 CGGCCG C^GGCCG
Eam1105I 67 GACTCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 52 CGGCCG C^GGCCG
Eco52I 52 CGGCCG C^GGCCG
EcoO109I 185 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRV 1054 GATATC GAT^ATC
EcoT22I 545 ATGCAT ATGCA^T
HaeII 295 GGCGCT [GA]GCGC^[CT]
Hin1I 1230 GACGTC G[GA]^CG[CT]C
HindIII 1177 AAGCTT A^AGCTT
HphI 433 GGTGA GGTGA(8/7)
MaeII 1231 ACGT A^CGT
MamI 1021 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 39 GCCGGC GCC^GGC
NciI 962 CCCGG CC^[GC]GG
NgoMIV 39 GCCGGC G^CCGGC
NsiI 545 ATGCAT ATGCA^T
NspI 1706 GCATGC [GA]CATG^[CT]
PaeR7I 12 CTCGAG C^TCGAG
PpuMI 185 GGGTCCT [GA]G^G[AT]CC[CT]
SfcI 1840 CTACAG C^T[GA][CT]AG
SpeI 1041 ACTAGT A^CTAGT
SphI 1706 GCATGC GCATG^C
XhoI 12 CTCGAG C^TCGAG
XmnI 1771 GAACCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AfaI 301 GTAC GT^AC
1110 GTAC
AocI 189 CCTGAGG CC^T[ACGT]AGG
499 CCTGAGG
AspHI 9 GAGCTC G[AT]GC[AT]^C
1720 GAGCTC
AspI 1453 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1608 GACTGTGTC
AvaII 186 GGTCC G^G[AT]CC
1527 GGACC
BalI 260 TGGCCA TGG^CCA
1392 TGGCCA
BbsI 1227 GAAGAC GAAGAC(2/6)
1645 GAAGAC
BbvI 17 GCTGC GCAGC(8/12)
1541 GCTGC
BciVI 1309 GGATAC GTATCC(6/5)
797 GTATCC
BpuAI 1227 GAAGAC GAAGAC(2/6)
1645 GAAGAC
BsiHKAI 9 GAGCTC G[AT]GC[AT]^C
1720 GAGCTC
BstYI 357 GGATCC [GA]^GATC[CT]
517 AGATCC
Bsu36I 189 CCTGAGG CC^T[ACGT]AGG
499 CCTGAGG
CfoI 296 GCGC GCG^C
1710 GCGC
Cfr13I 186 GGTCC G^G[ACGT]CC
1527 GGACC
DraIII 843 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
1898 CACAGTGTG
Eco81I 189 CCTGAGG CC^T[ACGT]AGG
499 CCTGAGG
HapII 40 CCGG C^CGG
963 CCGG
HhaI 296 GCGC GCG^C
1710 GCGC
HinP1I 296 GCGC G^CGC
1710 GCGC
HpaII 40 CCGG C^CGG
963 CCGG
MflI 357 GGATCC [GA]^GATC[CT]
517 AGATCC
MluNI 260 TGGCCA TGG^CCA
1392 TGGCCA
MscI 260 TGGCCA TGG^CCA
1392 TGGCCA
MspA1I 1539 CAGCTG C[AC]G^C[GT]G
1639 CAGCTG
MspI 40 CCGG C^CGG
963 CCGG
NcoI 860 CCATGG C^CATGG
1395 CCATGG
PflFI 1453 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1608 GACTGTGTC
PflMI 286 CCATTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
855 CCAGCCCATGG
PvuII 1539 CAGCTG CAG^CTG
1639 CAGCTG
RsaI 301 GTAC GT^AC
1110 GTAC
SacI 9 GAGCTC GAGCT^C
1720 GAGCTC
Sau96I 186 GGTCC G^G[ACGT]CC
1527 GGACC
SmlI 12 CTCGAG C^T[CT][GA]AG
1096 CTTAAG
TseI 17 GCTGC G^C[AT]GC
1541 GCTGC
Tth111I 1453 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1608 GACTGTGTC
Van91I 286 CCATTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
855 CCAGCCCATGG
XhoII 357 GGATCC [GA]^GATC[CT]
517 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
BanII BfaI BmyI BsaJI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I DdeI DpnII
DsaI EaeI EcoRI EcoRII EcoT14I
Fnu4HI FokI HaeIII HinfI ItaI
MaeI MaeIII MboI MboII MnlI
MseI MslI MvaI MwoI NdeII
NlaIII NlaIV PleI Sau3AI ScrFI
SfaNI SspI StyI TaqI TfiI
Tru9I Tsp45I Tsp509I TspRI TthHB8I
XcmI