(Circular) (Six-base) MAPSORT of: pFN21ASDA1073.seq Check: 9251 from: 1 to: 6658 pFN21ASDA1073 6658 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3257 4468 278 Size: 53 83 186 936 1721 1211 2468 Fragments arranged by size: 2468 1721 1211 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3951 4001 1937 Size: 2014 50 4594 Fragments arranged by size: 4594 2014 50 Acc65I G'GTAC_C Cuts at: 3979 3979 Size: 6658 AclI AA'CG_TT Cuts at: 1563 4785 5158 1563 Size: 3222 373 3063 Fragments arranged by size: 3222 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2788 3827 4721 6105 6373 1632 Size: 1156 1039 894 1384 268 1917 Fragments arranged by size: 1917 1384 1156 1039 894 268 AflII C'TTAA_G Cuts at: 820 1017 3119 820 Size: 197 2102 4359 Fragments arranged by size: 4359 2102 197 AflIII A'CryG_T Cuts at: 1203 5557 6534 1203 Size: 4354 977 1327 Fragments arranged by size: 4354 1327 977 AgeI A'CCGG_T Cuts at: 5489 5489 Size: 6658 AhdI GACnn_n'nnGTC Cuts at: 2095 5387 2095 Size: 3292 3366 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6658 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6658 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3054 3086 3054 Size: 32 6626 AlwNI CAG_nnn'CTG Cuts at: 2150 4401 5973 2150 Size: 2251 1572 2835 Fragments arranged by size: 2835 2251 1572 ApaLI G'TGCA_C Cuts at: 4715 5871 4715 Size: 1156 5502 ApoI r'AATT_y Cuts at: 1493 2125 2461 2500 2679 3518 3967 4193 4345 Size: 632 336 39 179 839 449 226 152 Cuts at: 4345 6454 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 839 632 449 336 226 179 152 39 AseI AT'TA_AT Cuts at: 160 1032 5212 160 Size: 872 4180 1606 Fragments arranged by size: 4180 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6658 AvaI C'yCGr_G Cuts at: 1870 1943 2035 3983 1870 Size: 73 92 1948 4545 Fragments arranged by size: 4545 1948 92 73 BamHI G'GATC_C Cuts at: 2380 3988 4355 2380 Size: 1608 367 4683 Fragments arranged by size: 4683 1608 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3979 5434 618 Size: 325 217 627 2192 1455 1842 Fragments arranged by size: 2192 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2036 3220 3747 3977 1391 Size: 645 1184 527 230 4072 Fragments arranged by size: 4072 1184 645 527 230 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3257 3675 928 Size: 971 1358 418 3911 Fragments arranged by size: 3911 1358 971 418 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3432 3466 4849 4883 1903 Size: 34 1495 34 1383 34 3678 Fragments arranged by size: 3678 1495 1383 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2831 3326 4550 5760 2831 Size: 495 1224 1210 3729 Fragments arranged by size: 3729 1224 1210 495 BclI T'GATC_A Cuts at: 1548 1548 Size: 6658 BfrBI ATG'CAT Cuts at: 2570 2570 Size: 6658 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3014 5269 136 Size: 107 122 71 631 605 1342 2255 1525 Fragments arranged by size: 2255 1525 1342 631 605 122 107 71 BglII A'GATC_T Cuts at: 6653 6653 Size: 6658 BlpI GC'TnA_GC Cuts at: 3297 4075 3297 Size: 778 5880 Bme1580I G_kGCm'C Cuts at: 1790 4719 5875 1790 Size: 2929 1156 2573 Fragments arranged by size: 2929 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6658 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2819 2950 4389 Size: 416 53 83 399 24 1402 131 1439 Cuts at: 4389 5347 442 Size: 958 1753 Fragments arranged by size: 1753 1439 1402 958 416 399 131 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6658 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2050 2050 Size: 6658 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2716 5318 1239 Size: 1477 2602 2579 Fragments arranged by size: 2602 2579 1477 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4783 5648 5946 4783 Size: 865 298 5495 Fragments arranged by size: 5495 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5321 882 Size: 617 3822 2219 Fragments arranged by size: 3822 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3955 4449 493 Size: 711 2751 494 2702 Fragments arranged by size: 2751 2702 711 494 BsaBI GATnn'nnATC Cuts at: 3048 6652 3048 Size: 3604 3054 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3254 4465 4847 5482 Size: 53 83 186 936 1721 1211 382 635 Cuts at: 5482 275 Size: 1451 Fragments arranged by size: 1721 1451 1211 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5091 5489 5763 5910 6321 5091 Size: 398 274 147 411 5428 Fragments arranged by size: 5428 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3116 3146 1178 Size: 30 165 30 1713 30 4690 Fragments arranged by size: 4690 1713 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6658 BseYI C'CCAG_C Cuts at: 2058 2081 5861 6357 2058 Size: 23 3780 496 2359 Fragments arranged by size: 3780 2359 496 23 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3903 1163 Size: 573 2167 3918 Fragments arranged by size: 3918 2167 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2078 4108 4869 5897 1540 Size: 275 186 77 2030 761 1028 2301 Fragments arranged by size: 2301 2030 1028 761 275 186 77 BsiHKAI G_wGCw'C Cuts at: 2036 3747 4719 4804 5875 2036 Size: 1711 972 85 1071 2819 Fragments arranged by size: 2819 1711 1071 972 85 BsmI GAATG_Cn' Cuts at: 4169 4262 4169 Size: 93 6565 BsmBI CGTCTCn'nnnn_ Cuts at: 2021 2021 Size: 6658 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2036 3220 3747 3977 4719 4804 5875 Size: 399 246 1184 527 230 742 85 1071 Cuts at: 5875 1391 Size: 2174 Fragments arranged by size: 2174 1184 1071 742 527 399 246 230 85 BspEI T'CCGG_A Cuts at: 6321 6321 Size: 6658 BspHI T'CATG_A Cuts at: 4547 6277 4547 Size: 1730 4928 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2598 4013 844 Size: 1754 1415 3489 Fragments arranged by size: 3489 1754 1415 BsrBI CCG'CTC Cuts at: 1394 1823 4545 1394 Size: 429 2722 3507 Fragments arranged by size: 3507 2722 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3268 5153 5327 65 Size: 1183 2020 1885 174 1396 Fragments arranged by size: 2020 1885 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2062 5302 5489 1700 Size: 110 252 3240 187 2869 Fragments arranged by size: 3240 2869 252 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6658 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6658 BssSI C'ACGA_G Cuts at: 4718 5730 4718 Size: 1012 5646 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6658 BstBI TT'CG_AA Cuts at: 2007 5462 2007 Size: 3455 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6658 BstYI r'GATC_y Cuts at: 1972 2380 2540 3988 4355 4740 4757 6209 6295 Size: 408 160 1448 367 385 17 1452 86 Cuts at: 6295 6307 6653 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1448 408 385 367 346 160 86 17 12 BstZ17I GTA'TAC Cuts at: 3952 3952 Size: 6658 Bsu36I CC'TnA_GG Cuts at: 2213 2523 2213 Size: 310 6348 BtgI C'CryG_G Cuts at: 513 1065 2022 2883 3418 3776 513 Size: 552 957 861 535 358 3395 Fragments arranged by size: 3395 957 861 552 535 358 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5919 Fragments arranged by size: 5919 683 56 BtsI GCAGTG_nn' Cuts at: 3280 4174 4968 4988 3280 Size: 894 794 20 4950 Fragments arranged by size: 4950 894 794 20 ClaI AT'CG_AT Cuts at: 1554 3397 1554 Size: 1843 4815 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6623 DraI TTT'AAA Cuts at: 3962 3962 Size: 6658 DraIII CAC_nnn'GTG Cuts at: 2871 3926 2871 Size: 1055 5603 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5665 809 Size: 507 4349 1802 Fragments arranged by size: 4349 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2075 2283 2987 Size: 54 1425 50 275 18 245 208 704 Cuts at: 2987 3415 4105 4994 6623 8 Size: 428 690 889 1629 43 Fragments arranged by size: 1629 1425 889 704 690 428 275 245 208 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2075 4105 1537 Size: 275 263 2030 4090 Fragments arranged by size: 4090 2030 275 263 EarI CTCTTCn'nnn_ Cuts at: 1353 4588 1353 Size: 3235 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2545 5245 5619 5765 387 Size: 1064 138 956 2700 374 146 1280 Fragments arranged by size: 2700 1280 1064 956 374 146 138 EcoICRI GAG'CTC Cuts at: 2034 3745 2034 Size: 1711 4947 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2716 2788 3827 4721 5318 6105 6373 Size: 393 1084 72 1039 894 597 787 268 Cuts at: 6373 1239 Size: 1524 Fragments arranged by size: 1524 1084 1039 894 787 597 393 268 72 EcoO109I rG'GnC_Cy Cuts at: 2209 2209 Size: 6658 EcoRI G'AATT_C Cuts at: 2125 2679 3518 3967 4345 2125 Size: 554 839 449 378 4438 Fragments arranged by size: 4438 839 554 449 378 EcoRV GAT'ATC Cuts at: 3079 3079 Size: 6658 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3635 3839 3635 Size: 204 6454 FspI TGC'GCA Cuts at: 5164 5164 Size: 6658 HaeII r_GCGC'y Cuts at: 1678 2322 5805 1678 Size: 644 3483 2531 Fragments arranged by size: 3483 2531 644 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2081 2113 2805 2837 3345 Size: 32 135 32 509 32 692 32 508 Cuts at: 3345 3377 5299 5331 5373 5405 1373 Size: 32 1922 32 42 32 2626 Fragments arranged by size: 2626 1922 692 509 508 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4002 4248 669 Size: 988 281 2064 246 3079 Fragments arranged by size: 3079 2064 988 281 246 HindIII A'AGCT_T Cuts at: 748 3200 748 Size: 2452 4206 HpaI GTT'AAC Cuts at: 4248 4248 Size: 6658 KpnI G_GTAC'C Cuts at: 3983 3983 Size: 6658 MfeI C'AATT_G Cuts at: 4257 4257 Size: 6658 MluI A'CGCG_T Cuts at: 6534 6534 Size: 6658 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2869 3306 5772 5956 1305 Size: 1564 437 2466 184 2007 Fragments arranged by size: 2466 2007 1564 437 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2285 3417 10 Size: 54 1425 343 453 1132 3251 Fragments arranged by size: 3251 1425 1132 453 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3273 3320 3438 4616 4975 5134 518 Size: 648 2107 47 118 1178 359 159 2042 Fragments arranged by size: 2107 2042 1178 648 359 159 118 47 MspA1I CmG'CkG Cuts at: 1606 3564 3664 4074 4751 5899 6144 1606 Size: 1958 100 410 677 1148 245 2120 Fragments arranged by size: 2120 1958 1148 677 410 245 100 NaeI GCC'GGC Cuts at: 2064 2064 Size: 6658 NcoI C'CATG_G Cuts at: 513 1065 2022 2883 3418 513 Size: 552 957 861 535 3753 Fragments arranged by size: 3753 957 861 552 535 NdeI CA'TA_TG Cuts at: 387 387 Size: 6658 NgoMIV G'CCGG_C Cuts at: 2062 2062 Size: 6658 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6658 NotI GC'GGCC_GC Cuts at: 4105 4105 Size: 6658 NsiI A_TGCA'T Cuts at: 2572 2572 Size: 6658 NspI r_CATG'y Cuts at: 1132 1229 3733 4016 5561 1132 Size: 97 2504 283 1545 2229 Fragments arranged by size: 2504 2229 1545 283 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6658 PciI A'CATG_T Cuts at: 5557 5557 Size: 6658 PflMI CCAn_nnn'nTGG Cuts at: 1108 2315 2884 1108 Size: 1207 569 4882 Fragments arranged by size: 4882 1207 569 PfoI T'CCnGG_A Cuts at: 2260 3072 2260 Size: 812 5846 PmeI GTTT'AAAC Cuts at: 3962 3962 Size: 6658 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4727 4759 6240 6272 4727 Size: 32 1481 32 5113 Fragments arranged by size: 5113 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2209 2209 Size: 6658 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6658 PsiI TTA'TAA Cuts at: 4228 4228 Size: 6658 PspXI vC'TCGA_Gb Cuts at: 2035 2035 Size: 6658 PstI C_TGCA'G Cuts at: 830 4010 830 Size: 3180 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6658 PvuII CAG'CTG Cuts at: 3564 3664 3564 Size: 100 6558 SacI G_AGCT'C Cuts at: 2036 3747 2036 Size: 1711 4947 SalI G'TCGA_C Cuts at: 1936 4000 1936 Size: 2064 4594 SbfI CC_TGCA'GG Cuts at: 4010 4010 Size: 6658 ScaI AGT'ACT Cuts at: 4906 6618 4906 Size: 1712 4946 SfcI C'TryA_G Cuts at: 826 1045 3863 4006 5141 5822 6013 826 Size: 219 2818 143 1135 681 191 1471 Fragments arranged by size: 2818 1471 1135 681 219 191 143 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6658 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6658 SmaI CCC'GGG Cuts at: 1872 3985 1872 Size: 2113 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2035 3119 4762 5663 5925 820 Size: 197 926 92 1084 1643 901 262 1553 Fragments arranged by size: 1643 1553 1084 926 901 262 197 92 SnaBI TAC'GTA Cuts at: 493 3955 4449 493 Size: 3462 494 2702 Fragments arranged by size: 3462 2702 494 SpeI A'CTAG_T Cuts at: 152 3064 152 Size: 2912 3746 SphI G_CATG'C Cuts at: 1132 3733 4016 1132 Size: 2601 283 3774 Fragments arranged by size: 3774 2601 283 SspI AAT'ATT Cuts at: 5 52 2272 2770 3088 4582 5 Size: 47 2220 498 318 1494 2081 Fragments arranged by size: 2220 2081 1494 498 318 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6658 StyI C'CwwG_G Cuts at: 513 1065 2022 2407 2883 3418 4097 513 Size: 552 957 385 476 535 679 3074 Fragments arranged by size: 3074 957 679 552 535 476 385 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2625 3739 4685 4702 4855 5040 5485 Size: 1255 1045 1114 946 17 153 185 445 Cuts at: 5485 325 Size: 1498 Fragments arranged by size: 1498 1255 1114 1045 946 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4904 6616 96 Size: 274 80 33 51 1443 2927 1712 138 Fragments arranged by size: 2927 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6618 6650 6618 Size: 32 6626 Tth111I GACn'n_nGTC Cuts at: 1316 3479 3634 1316 Size: 2163 155 4340 Fragments arranged by size: 4340 2163 155 XbaI T'CTAG_A Cuts at: 3994 3994 Size: 6658 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2691 2832 2885 3322 2691 Size: 141 53 437 6027 Fragments arranged by size: 6027 437 141 53 XhoI C'TCGA_G Cuts at: 1943 2035 1943 Size: 92 6566 XmaI C'CCGG_G Cuts at: 1870 3983 1870 Size: 2113 4545 XmnI GAAnn'nnTTC Cuts at: 3798 4787 3798 Size: 989 5669 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3255 4466 276 Size: 53 83 186 936 1721 1211 2468 Fragments arranged by size: 2468 1721 1211 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BstXI EcoNI FseI FspAI KasI NarI NruI PacI PmlI PspOMI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI