(Circular) (Six-base) MAPSORT of: pFN21ASDA1001.seq Check: 1740 from: 1 to: 6304 pFN21ASDA1001 6304 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2279 2279 Size: 6304 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3195 3489 4114 278 Size: 53 83 186 936 1659 294 625 2468 Fragments arranged by size: 2468 1659 936 625 294 186 83 53 AccI GT'mk_AC Cuts at: 1937 3647 1937 Size: 1710 4594 Acc65I G'GTAC_C Cuts at: 3625 3625 Size: 6304 AclI AA'CG_TT Cuts at: 1563 4431 4804 1563 Size: 2868 373 3063 Fragments arranged by size: 3063 2868 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3528 4367 5751 6019 1632 Size: 1896 839 1384 268 1917 Fragments arranged by size: 1917 1896 1384 839 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6107 AflIII A'CryG_T Cuts at: 1203 5203 6180 1203 Size: 4000 977 1327 Fragments arranged by size: 4000 1327 977 AgeI A'CCGG_T Cuts at: 3323 5135 3323 Size: 1812 4492 AhdI GACnn_n'nnGTC Cuts at: 5033 5033 Size: 6304 AleI CACnn'nnGTG Cuts at: 1166 2351 2615 1166 Size: 1185 264 4855 Fragments arranged by size: 4855 1185 264 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6304 AlwNI CAG_nnn'CTG Cuts at: 3416 4047 5619 3416 Size: 631 1572 4101 Fragments arranged by size: 4101 1572 631 ApaLI G'TGCA_C Cuts at: 4361 5517 4361 Size: 1156 5148 ApoI r'AATT_y Cuts at: 1493 3613 3839 3991 6100 1493 Size: 2120 226 152 2109 1697 Fragments arranged by size: 2120 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4858 160 Size: 872 3826 1606 Fragments arranged by size: 3826 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6304 AvaI C'yCGr_G Cuts at: 1870 1943 2283 3461 3629 1870 Size: 73 340 1178 168 4545 Fragments arranged by size: 4545 1178 340 168 73 BamHI G'GATC_C Cuts at: 3634 4001 3634 Size: 367 5937 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3042 3625 5080 618 Size: 325 217 627 1255 583 1455 1842 Fragments arranged by size: 1842 1455 1255 627 583 325 217 BanII G_rGCy'C Cuts at: 1391 2841 2944 3280 3623 1391 Size: 1450 103 336 343 4072 Fragments arranged by size: 4072 1450 343 336 103 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3405 928 Size: 971 1506 3827 Fragments arranged by size: 3827 1506 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4495 4529 1903 Size: 34 2558 34 3678 Fragments arranged by size: 3678 2558 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4196 5406 4196 Size: 1210 5094 BclI T'GATC_A Cuts at: 1548 1548 Size: 6304 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2310 4915 136 Size: 107 122 71 631 605 638 2605 1525 Fragments arranged by size: 2605 1525 638 631 605 122 107 71 BglII A'GATC_T Cuts at: 6299 6299 Size: 6304 BlpI GC'TnA_GC Cuts at: 3721 3721 Size: 6304 Bme1580I G_kGCm'C Cuts at: 1790 2781 3045 3231 3423 4365 5521 1790 Size: 991 264 186 192 942 1156 2573 Fragments arranged by size: 2573 1156 991 942 264 192 186 BmgBI CAC'GTC Cuts at: 1318 2617 1318 Size: 1299 5005 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2194 2427 2701 Size: 416 53 83 399 24 777 233 274 Cuts at: 2701 4035 4993 442 Size: 1334 958 1753 Fragments arranged by size: 1753 1334 958 777 416 399 274 233 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2960 1056 Size: 1904 4400 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2517 3285 3317 3502 4964 1239 Size: 1278 768 32 185 1462 2579 Fragments arranged by size: 2579 1462 1278 768 185 32 Bpu10I CC'TnA_GC Cuts at: 3360 3360 Size: 6304 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2689 3150 4429 5294 5592 2689 Size: 461 1279 865 298 3401 Fragments arranged by size: 3401 1279 865 461 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2369 4967 882 Size: 617 870 2598 2219 Fragments arranged by size: 2598 2219 870 617 BsaAI yAC'GTr Cuts at: 493 1204 2776 3601 4095 493 Size: 711 1572 825 494 2702 Fragments arranged by size: 2702 1572 825 711 494 BsaBI GATnn'nnATC Cuts at: 6298 6298 Size: 6304 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3192 3486 4111 4493 Size: 53 83 186 936 1659 294 625 382 Cuts at: 4493 5128 275 Size: 635 1451 Fragments arranged by size: 1659 1451 936 635 625 382 294 186 83 53 BsaWI w'CCGG_w Cuts at: 3323 4737 5135 5409 5556 5967 3323 Size: 1414 398 274 147 411 3660 Fragments arranged by size: 3660 1414 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2515 2545 3500 3530 1178 Size: 30 165 30 1112 30 955 30 3952 Fragments arranged by size: 3952 1112 955 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6304 BseYI C'CCAG_C Cuts at: 3052 5507 6003 3052 Size: 2455 496 3353 Fragments arranged by size: 3353 2455 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2853 1163 Size: 573 1117 4614 Fragments arranged by size: 4614 1117 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2305 3754 4515 5543 1540 Size: 275 186 304 1449 761 1028 2301 Fragments arranged by size: 2301 1449 1028 761 304 275 186 BsiHKAI G_wGCw'C Cuts at: 3207 3353 4365 4450 5521 3207 Size: 146 1012 85 1071 3990 Fragments arranged by size: 3990 1071 1012 146 85 BsmI GAATG_Cn' Cuts at: 3815 3908 3815 Size: 93 6211 BsmBI CGTCTCn'nnnn_ Cuts at: 3199 3199 Size: 6304 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2781 2841 2944 3045 3207 3231 3280 Size: 399 991 60 103 101 162 24 49 Cuts at: 3280 3353 3423 3623 4365 4450 5521 1391 Size: 73 70 200 742 85 1071 2174 Fragments arranged by size: 2174 1071 991 742 399 200 162 103 101 85 73 70 60 49 24 BspEI T'CCGG_A Cuts at: 5967 5967 Size: 6304 BspHI T'CATG_A Cuts at: 4193 5923 4193 Size: 1730 4574 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2279 3659 844 Size: 1435 1380 3489 Fragments arranged by size: 3489 1435 1380 BsrBI CCG'CTC Cuts at: 1394 1823 2368 4191 1394 Size: 429 545 1823 3507 Fragments arranged by size: 3507 1823 545 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2326 3498 4799 4973 65 Size: 1183 1078 1172 1301 174 1396 Fragments arranged by size: 1396 1301 1183 1172 1078 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2300 2304 2659 3213 3323 4948 5135 Size: 110 490 4 355 554 110 1625 187 Cuts at: 5135 1700 Size: 2869 Fragments arranged by size: 2869 1625 554 490 355 187 110 110 4 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6304 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6304 BssSI C'ACGA_G Cuts at: 4364 5376 4364 Size: 1012 5292 BstAPI GCAn_nnn'nTGC Cuts at: 794 3235 794 Size: 2441 3863 BstBI TT'CG_AA Cuts at: 2007 5108 2007 Size: 3101 3203 BstEII G'GTnAC_C Cuts at: 1184 2168 1184 Size: 984 5320 BstYI r'GATC_y Cuts at: 1972 3634 4001 4386 4403 5855 5941 5953 6299 Size: 1662 367 385 17 1452 86 12 346 Cuts at: 6299 1972 Size: 1977 Fragments arranged by size: 1977 1662 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2449 2473 2810 2936 513 Size: 552 957 427 24 337 126 3881 Fragments arranged by size: 3881 957 552 427 337 126 24 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5565 Fragments arranged by size: 5565 683 56 BtsI GCAGTG_nn' Cuts at: 2202 2971 3099 3820 4614 4634 2202 Size: 769 128 721 794 20 3872 Fragments arranged by size: 3872 794 769 721 128 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6304 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2455 2490 2534 2569 529 Size: 35 1891 35 44 35 4264 Fragments arranged by size: 4264 1891 44 35 35 35 DraI TTT'AAA Cuts at: 3608 3608 Size: 6304 DraIII CAC_nnn'GTG Cuts at: 2573 2893 3235 2573 Size: 320 342 5642 Fragments arranged by size: 5642 342 320 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5311 809 Size: 507 3995 1802 Fragments arranged by size: 3995 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2148 2302 3211 Size: 54 1425 50 275 18 318 154 909 Cuts at: 3211 3751 4640 6269 8 Size: 540 889 1629 43 Fragments arranged by size: 1629 1425 909 889 540 318 275 154 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2302 3751 1537 Size: 275 490 1449 4090 Fragments arranged by size: 4090 1449 490 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4234 1353 Size: 2881 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3281 4891 5265 5411 387 Size: 1064 138 1692 1610 374 146 1280 Fragments arranged by size: 1692 1610 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2517 3285 3317 3502 3528 4367 4964 Size: 393 885 768 32 185 26 839 597 Cuts at: 4964 5751 6019 1239 Size: 787 268 1524 Fragments arranged by size: 1524 885 839 787 768 597 393 268 185 32 26 EcoNI CCTnn'n_nnAGG Cuts at: 3149 3149 Size: 6304 EcoO109I rG'GnC_Cy Cuts at: 2739 2972 3049 3356 3538 2739 Size: 233 77 307 182 5505 Fragments arranged by size: 5505 307 233 182 77 EcoRI G'AATT_C Cuts at: 3613 3991 3613 Size: 378 5926 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2149 2149 Size: 6304 FspI TGC'GCA Cuts at: 4810 4810 Size: 6304 HaeII r_GCGC'y Cuts at: 1678 2810 3178 5451 1678 Size: 1132 368 2273 2531 Fragments arranged by size: 2531 2273 1132 368 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2567 2599 2839 2871 3500 Size: 32 135 32 995 32 240 32 629 Cuts at: 3500 3532 4945 4977 5019 5051 1373 Size: 32 1413 32 42 32 2626 Fragments arranged by size: 2626 1413 995 629 240 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2884 3648 3894 669 Size: 988 281 946 764 246 3079 Fragments arranged by size: 3079 988 946 764 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6304 HpaI GTT'AAC Cuts at: 3894 3894 Size: 6304 KpnI G_GTAC'C Cuts at: 3629 3629 Size: 6304 MfeI C'AATT_G Cuts at: 3903 3903 Size: 6304 MluI A'CGCG_T Cuts at: 6180 6180 Size: 6304 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3155 5418 5602 1305 Size: 1850 2263 184 2007 Fragments arranged by size: 2263 2007 1850 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4482 Fragments arranged by size: 4482 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2351 2615 2774 4262 4621 4780 518 Size: 648 1185 264 159 1488 359 159 2042 Fragments arranged by size: 2042 1488 1185 648 359 264 159 159 MspA1I CmG'CkG Cuts at: 1606 2261 2733 3255 3263 3585 3720 4397 5545 Size: 655 472 522 8 322 135 677 1148 Cuts at: 5545 5790 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 655 522 472 322 245 135 8 NaeI GCC'GGC Cuts at: 2306 2306 Size: 6304 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4795 Fragments arranged by size: 4795 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6304 NgoMIV G'CCGG_C Cuts at: 2304 2304 Size: 6304 NheI G'CTAG_C Cuts at: 1052 2956 1052 Size: 1904 4400 NotI GC'GGCC_GC Cuts at: 3751 3751 Size: 6304 NspI r_CATG'y Cuts at: 1132 1229 2773 3662 5207 1132 Size: 97 1544 889 1545 2229 Fragments arranged by size: 2229 1545 1544 889 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6304 PciI A'CATG_T Cuts at: 5203 5203 Size: 6304 PflMI CCAn_nnn'nTGG Cuts at: 1108 2028 2515 1108 Size: 920 487 4897 Fragments arranged by size: 4897 920 487 PmeI GTTT'AAAC Cuts at: 3608 3608 Size: 6304 PmlI CAC'GTG Cuts at: 2776 2776 Size: 6304 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4373 4405 5886 5918 4373 Size: 32 1481 32 4759 Fragments arranged by size: 4759 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2972 3049 3538 2972 Size: 77 489 5738 Fragments arranged by size: 5738 489 77 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6304 PsiI TTA'TAA Cuts at: 3874 3874 Size: 6304 PstI C_TGCA'G Cuts at: 830 2400 3285 3369 3656 830 Size: 1570 885 84 287 3478 Fragments arranged by size: 3478 1570 885 287 84 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6304 PvuII CAG'CTG Cuts at: 2261 3263 2261 Size: 1002 5302 SalI G'TCGA_C Cuts at: 1936 3646 1936 Size: 1710 4594 SanDI GG'GwC_CC Cuts at: 2972 3049 2972 Size: 77 6227 SbfI CC_TGCA'GG Cuts at: 3656 3656 Size: 6304 ScaI AGT'ACT Cuts at: 4552 6264 4552 Size: 1712 4592 SfcI C'TryA_G Cuts at: 826 1045 2396 3281 3365 3652 4787 5468 5659 Size: 219 1351 885 84 287 1135 681 191 Cuts at: 5659 826 Size: 1471 Fragments arranged by size: 1471 1351 1135 885 681 287 219 191 84 SfiI GGCCn_nnn'nGGCC Cuts at: 2310 2310 Size: 6304 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6304 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6304 SmaI CCC'GGG Cuts at: 1872 2285 3631 1872 Size: 413 1346 4545 Fragments arranged by size: 4545 1346 413 SmlI C'TyrA_G Cuts at: 820 1017 1943 2668 3165 4408 5309 5571 820 Size: 197 926 725 497 1243 901 262 1553 Fragments arranged by size: 1553 1243 926 901 725 497 262 197 SnaBI TAC'GTA Cuts at: 493 3601 4095 493 Size: 3108 494 2702 Fragments arranged by size: 3108 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6304 SphI G_CATG'C Cuts at: 1132 3662 1132 Size: 2530 3774 SspI AAT'ATT Cuts at: 5 52 4228 5 Size: 47 4176 2081 Fragments arranged by size: 4176 2081 47 StuI AGG'CCT Cuts at: 1517 2361 3311 1517 Size: 844 950 4510 Fragments arranged by size: 4510 950 844 StyI C'CwwG_G Cuts at: 513 1065 2022 2382 3743 513 Size: 552 957 360 1361 3074 Fragments arranged by size: 3074 1361 957 552 360 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4331 4348 4501 4686 5131 325 Size: 1255 2751 17 153 185 445 1498 Fragments arranged by size: 2751 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2522 4550 6262 Size: 274 80 33 51 1443 545 2028 1712 Cuts at: 6262 96 Size: 138 Fragments arranged by size: 2028 1712 1443 545 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6264 6296 6264 Size: 32 6272 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6304 XbaI T'CTAG_A Cuts at: 3640 3640 Size: 6304 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6304 XmaI C'CCGG_G Cuts at: 1870 2283 3629 1870 Size: 413 1346 4545 Fragments arranged by size: 4545 1346 413 XmnI GAAnn'nnTTC Cuts at: 4433 4433 Size: 6304 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3193 3487 4112 276 Size: 53 83 186 936 1659 294 625 2468 Fragments arranged by size: 2468 1659 936 625 294 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfoI SrfI SwaI XcmI