(Circular) (Six-base) MAPSORT of: pFN21ASDA0979.seq Check: 3347 from: 1 to: 9070 pFN21ASDA0979 9070 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6880 278 Size: 53 83 186 936 5344 2468 Fragments arranged by size: 5344 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2556 6413 1937 Size: 619 3857 4594 Fragments arranged by size: 4594 3857 619 Acc65I G'GTAC_C Cuts at: 2632 6391 2632 Size: 3759 5311 AclI AA'CG_TT Cuts at: 1563 7197 7570 1563 Size: 5634 373 3063 Fragments arranged by size: 5634 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2190 2199 2322 2708 3771 4961 5775 7133 Size: 558 9 123 386 1063 1190 814 1358 Cuts at: 7133 8517 8785 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1358 1190 1063 814 558 386 268 123 9 AflII C'TTAA_G Cuts at: 820 1017 3980 4573 820 Size: 197 2963 593 5317 Fragments arranged by size: 5317 2963 593 197 AflIII A'CryG_T Cuts at: 1203 7969 8946 1203 Size: 6766 977 1327 Fragments arranged by size: 6766 1327 977 AgeI A'CCGG_T Cuts at: 7901 7901 Size: 9070 AhdI GACnn_n'nnGTC Cuts at: 7799 7799 Size: 9070 AleI CACnn'nnGTG Cuts at: 1166 3674 1166 Size: 2508 6562 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 9070 AlwNI CAG_nnn'CTG Cuts at: 2743 3296 4082 5798 6813 8385 2743 Size: 553 786 1716 1015 1572 3428 Fragments arranged by size: 3428 1716 1572 1015 786 553 ApaLI G'TGCA_C Cuts at: 3854 7127 8283 3854 Size: 3273 1156 4641 Fragments arranged by size: 4641 3273 1156 ApoI r'AATT_y Cuts at: 1493 2478 2647 2855 3026 3264 3625 3665 4092 Size: 985 169 208 171 238 361 40 427 Cuts at: 4092 4497 5332 6379 6605 6757 8866 1493 Size: 405 835 1047 226 152 2109 1697 Fragments arranged by size: 2109 1697 1047 985 835 427 405 361 238 226 208 171 169 152 40 AseI AT'TA_AT Cuts at: 160 1032 5105 7624 160 Size: 872 4073 2519 1606 Fragments arranged by size: 4073 2519 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9070 AvaI C'yCGr_G Cuts at: 1870 1943 6395 1870 Size: 73 4452 4545 Fragments arranged by size: 4545 4452 73 BamHI G'GATC_C Cuts at: 6400 6767 6400 Size: 367 8703 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2632 6391 7846 618 Size: 325 217 627 845 3759 1455 1842 Fragments arranged by size: 3759 1842 1455 845 627 325 217 BanII G_rGCy'C Cuts at: 1391 2390 2570 2808 4891 6389 1391 Size: 999 180 238 2083 1498 4072 Fragments arranged by size: 4072 2083 1498 999 238 180 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 5583 6157 928 Size: 971 3684 574 3841 Fragments arranged by size: 3841 3684 971 574 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7261 7295 1903 Size: 34 5324 34 3678 Fragments arranged by size: 5324 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2371 2605 4229 6962 8172 2371 Size: 234 1624 2733 1210 3269 Fragments arranged by size: 3269 2733 1624 1210 234 BclI T'GATC_A Cuts at: 1548 3181 3822 3949 4357 1548 Size: 1633 641 127 408 6261 Fragments arranged by size: 6261 1633 641 408 127 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2965 7681 136 Size: 107 122 71 631 605 1293 4716 1525 Fragments arranged by size: 4716 1525 1293 631 605 122 107 71 BglII A'GATC_T Cuts at: 4408 9065 4408 Size: 4657 4413 BlpI GC'TnA_GC Cuts at: 6487 6487 Size: 9070 Bme1580I G_kGCm'C Cuts at: 1790 3858 4855 7131 8287 1790 Size: 2068 997 2276 1156 2573 Fragments arranged by size: 2573 2276 2068 1156 997 BmgBI CAC'GTC Cuts at: 1318 4652 1318 Size: 3334 5736 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4243 6801 7759 Size: 416 53 83 399 24 2826 2558 958 Cuts at: 7759 442 Size: 1753 Fragments arranged by size: 2826 2558 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3036 1056 Size: 1980 7090 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2715 4905 2715 Size: 2190 6880 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5970 7730 1239 Size: 4731 1760 2579 Fragments arranged by size: 4731 2579 1760 Bpu10I CC'TnA_GC Cuts at: 3648 3648 Size: 9070 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2213 2438 2685 4853 7195 8060 8358 2213 Size: 225 247 2168 2342 865 298 2925 Fragments arranged by size: 2925 2342 2168 865 298 247 225 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4238 7733 882 Size: 617 2739 3495 2219 Fragments arranged by size: 3495 2739 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 6367 6861 493 Size: 711 5163 494 2702 Fragments arranged by size: 5163 2702 711 494 BsaBI GATnn'nnATC Cuts at: 9064 9064 Size: 9070 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6877 7259 7894 275 Size: 53 83 186 936 5344 382 635 1451 Fragments arranged by size: 5344 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 7503 7901 8175 8322 8733 7503 Size: 398 274 147 411 7840 Fragments arranged by size: 7840 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 8845 Fragments arranged by size: 8845 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2123 2897 5714 6011 6014 6320 6323 1186 Size: 937 774 2817 297 3 306 3 3933 Fragments arranged by size: 3933 2817 937 774 306 297 3 3 BseYI C'CCAG_C Cuts at: 2487 2847 4667 8273 8769 2487 Size: 360 1820 3606 496 2788 Fragments arranged by size: 3606 2788 1820 496 360 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4068 1163 Size: 573 2332 6165 Fragments arranged by size: 6165 2332 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6520 7281 8309 1540 Size: 275 186 4519 761 1028 2301 Fragments arranged by size: 4519 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2570 2808 3858 3883 4891 6079 7131 7216 8287 Size: 238 1050 25 1008 1188 1052 85 1071 Cuts at: 8287 2570 Size: 3353 Fragments arranged by size: 3353 1188 1071 1052 1050 1008 238 85 25 BsmI GAATG_Cn' Cuts at: 4769 6581 6674 4769 Size: 1812 93 7165 Fragments arranged by size: 7165 1812 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2113 5619 2113 Size: 3506 5564 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2390 2570 2808 3858 3883 4855 4891 Size: 399 600 180 238 1050 25 972 36 Cuts at: 4891 6079 6389 7131 7216 8287 1391 Size: 1188 310 742 85 1071 2174 Fragments arranged by size: 2174 1188 1071 1050 972 742 600 399 310 238 180 85 36 25 BspEI T'CCGG_A Cuts at: 8733 8733 Size: 9070 BspHI T'CATG_A Cuts at: 3045 3657 4416 6959 8689 3045 Size: 612 759 2543 1730 3426 Fragments arranged by size: 3426 2543 1730 759 612 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2644 2973 3956 6425 844 Size: 1800 329 983 2469 3489 Fragments arranged by size: 3489 2469 1800 983 329 BsrBI CCG'CTC Cuts at: 1394 1823 6957 1394 Size: 429 5134 3507 Fragments arranged by size: 5134 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2424 2479 2878 4907 5497 7565 7739 Size: 1183 1176 55 399 2029 590 2068 174 Cuts at: 7739 65 Size: 1396 Fragments arranged by size: 2068 2029 1396 1183 1176 590 399 174 55 BsrFI r'CCGG_y Cuts at: 1700 1810 7714 7901 1700 Size: 110 5904 187 2869 Fragments arranged by size: 5904 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 5217 96 Size: 5121 3949 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9070 BssSI C'ACGA_G Cuts at: 7130 8142 7130 Size: 1012 8058 BstAPI GCAn_nnn'nTGC Cuts at: 794 4082 4850 794 Size: 3288 768 5014 Fragments arranged by size: 5014 3288 768 BstBI TT'CG_AA Cuts at: 2007 7874 2007 Size: 5867 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9070 BstYI r'GATC_y Cuts at: 1972 3393 4288 4408 4867 6400 6767 7152 7169 Size: 1421 895 120 459 1533 367 385 17 Cuts at: 7169 8621 8707 8719 9065 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1533 1452 1421 895 459 385 367 346 120 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3108 5817 3108 Size: 2709 6361 BtgI C'CryG_G Cuts at: 513 1065 2022 513 Size: 552 957 7561 Fragments arranged by size: 7561 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8331 Fragments arranged by size: 8331 683 56 BtsI GCAGTG_nn' Cuts at: 2975 5816 6586 7380 7400 2975 Size: 2841 770 794 20 4645 Fragments arranged by size: 4645 2841 794 770 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9070 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9035 DraI TTT'AAA Cuts at: 2193 2652 2860 3480 6374 2193 Size: 459 208 620 2894 4889 Fragments arranged by size: 4889 2894 620 459 208 DraIII CAC_nnn'GTG Cuts at: 5226 6064 5226 Size: 838 8232 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4191 8077 809 Size: 507 2875 3886 1802 Fragments arranged by size: 3886 2875 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2524 6517 7406 Size: 54 1425 50 275 18 694 3993 889 Cuts at: 7406 9035 8 Size: 1629 43 Fragments arranged by size: 3993 1629 1425 889 694 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6517 1537 Size: 275 4705 4090 Fragments arranged by size: 4705 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2684 4116 4879 4983 5928 7000 1353 Size: 1331 1432 763 104 945 1072 3423 Fragments arranged by size: 3423 1432 1331 1072 945 763 104 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2058 2999 5862 7657 8031 8177 Size: 1064 138 469 941 2863 1795 374 146 Cuts at: 8177 387 Size: 1280 Fragments arranged by size: 2863 1795 1280 1064 941 469 374 146 138 EcoICRI GAG'CTC Cuts at: 2568 2806 4889 2568 Size: 238 2083 6749 Fragments arranged by size: 6749 2083 238 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2190 2199 2322 2708 3771 4961 5775 Size: 393 558 9 123 386 1063 1190 814 Cuts at: 5775 5970 7133 7730 8517 8785 1239 Size: 195 1163 597 787 268 1524 Fragments arranged by size: 1524 1190 1163 1063 814 787 597 558 393 386 268 195 123 9 EcoO109I rG'GnC_Cy Cuts at: 4184 4184 Size: 9070 EcoRI G'AATT_C Cuts at: 6379 6757 6379 Size: 378 8692 EcoRV GAT'ATC Cuts at: 2995 2995 Size: 9070 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3850 5099 3850 Size: 1249 7821 FspI TGC'GCA Cuts at: 7576 7576 Size: 9070 HaeII r_GCGC'y Cuts at: 1678 2890 8217 1678 Size: 1212 5327 2531 Fragments arranged by size: 5327 2531 1212 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2084 2116 2150 2182 2281 Size: 32 135 32 512 32 34 32 99 Cuts at: 2281 2313 2579 2611 3385 3417 3854 3886 4273 Size: 32 266 32 774 32 437 32 387 Cuts at: 4273 4305 7711 7743 7785 7817 1373 Size: 32 3406 32 42 32 2626 Fragments arranged by size: 3406 2626 774 512 437 387 266 135 99 42 34 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5425 6414 6660 669 Size: 988 281 3487 989 246 3079 Fragments arranged by size: 3487 3079 989 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 9070 HpaI GTT'AAC Cuts at: 5425 6660 5425 Size: 1235 7835 KpnI G_GTAC'C Cuts at: 2636 6395 2636 Size: 3759 5311 MfeI C'AATT_G Cuts at: 6111 6669 6111 Size: 558 8512 MluI A'CGCG_T Cuts at: 8946 8946 Size: 9070 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3553 3752 3968 5750 8184 8368 1305 Size: 2248 199 216 1782 2434 184 2007 Fragments arranged by size: 2434 2248 2007 1782 216 199 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2526 10 Size: 54 1425 343 694 6554 Fragments arranged by size: 6554 1425 694 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3674 4602 7028 7387 7546 518 Size: 648 2508 928 2426 359 159 2042 Fragments arranged by size: 2508 2426 2042 928 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3152 3296 4955 6486 7163 8311 8556 1606 Size: 1546 144 1659 1531 677 1148 245 2120 Fragments arranged by size: 2120 1659 1546 1531 1148 677 245 144 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 7561 Fragments arranged by size: 7561 957 552 NdeI CA'TA_TG Cuts at: 387 2450 2544 2665 387 Size: 2063 94 121 6792 Fragments arranged by size: 6792 2063 121 94 NheI G'CTAG_C Cuts at: 1052 3032 1052 Size: 1980 7090 NotI GC'GGCC_GC Cuts at: 6517 6517 Size: 9070 NspI r_CATG'y Cuts at: 1132 1229 2786 3735 4952 6428 7973 1132 Size: 97 1557 949 1217 1476 1545 2229 Fragments arranged by size: 2229 1557 1545 1476 1217 949 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 9070 PciI A'CATG_T Cuts at: 7969 7969 Size: 9070 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 9070 PfoI T'CCnGG_A Cuts at: 3813 5386 5521 3813 Size: 1573 135 7362 Fragments arranged by size: 7362 1573 135 PmeI GTTT'AAAC Cuts at: 6374 6374 Size: 9070 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2011 2043 7139 7171 8652 8684 2011 Size: 32 5096 32 1481 32 2397 Fragments arranged by size: 5096 2397 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 4184 4184 Size: 9070 PshAI GACnn'nnGTC Cuts at: 1613 4490 1613 Size: 2877 6193 PsiI TTA'TAA Cuts at: 2351 2516 3270 6640 2351 Size: 165 754 3370 4781 Fragments arranged by size: 4781 3370 754 165 PstI C_TGCA'G Cuts at: 830 6422 830 Size: 5592 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9070 PvuII CAG'CTG Cuts at: 3152 3296 4955 3152 Size: 144 1659 7267 Fragments arranged by size: 7267 1659 144 SacI G_AGCT'C Cuts at: 2570 2808 4891 2570 Size: 238 2083 6749 Fragments arranged by size: 6749 2083 238 SalI G'TCGA_C Cuts at: 1936 6412 1936 Size: 4476 4594 SapI GCTCTTCn'nnn_ Cuts at: 4879 5928 4879 Size: 1049 8021 SbfI CC_TGCA'GG Cuts at: 6422 6422 Size: 9070 ScaI AGT'ACT Cuts at: 3717 5263 7318 9030 3717 Size: 1546 2055 1712 3757 Fragments arranged by size: 3757 2055 1712 1546 SfcI C'TryA_G Cuts at: 826 1045 3432 6321 6418 7553 8234 8425 826 Size: 219 2387 2889 97 1135 681 191 1471 Fragments arranged by size: 2889 2387 1471 1135 681 219 191 97 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9070 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9070 SmaI CCC'GGG Cuts at: 1872 6397 1872 Size: 4525 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2228 2417 2700 3980 4573 4832 Size: 197 926 285 189 283 1280 593 259 Cuts at: 4832 7174 8075 8337 820 Size: 2342 901 262 1553 Fragments arranged by size: 2342 1553 1280 926 901 593 285 283 262 259 197 189 SnaBI TAC'GTA Cuts at: 493 6367 6861 493 Size: 5874 494 2702 Fragments arranged by size: 5874 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 9070 SphI G_CATG'C Cuts at: 1132 4952 6428 1132 Size: 3820 1476 3774 Fragments arranged by size: 3820 3774 1476 SspI AAT'ATT Cuts at: 5 52 2815 4593 5103 5161 6994 5 Size: 47 2763 1778 510 58 1833 2081 Fragments arranged by size: 2763 2081 1833 1778 510 58 47 StuI AGG'CCT Cuts at: 1517 4809 1517 Size: 3292 5778 StyI C'CwwG_G Cuts at: 513 1065 2022 5182 6509 513 Size: 552 957 3160 1327 3074 Fragments arranged by size: 3160 3074 1327 957 552 SwaI ATTT'AAAT Cuts at: 2860 3480 2860 Size: 620 8450 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2501 7097 7114 7267 7452 7897 325 Size: 1255 921 4596 17 153 185 445 1498 Fragments arranged by size: 4596 1498 1255 921 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3715 5044 5217 Size: 274 80 33 51 1443 1738 1329 173 Cuts at: 5217 5261 7316 9028 96 Size: 44 2055 1712 138 Fragments arranged by size: 2055 1738 1712 1443 1329 274 173 138 80 51 44 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5599 5631 9030 9062 5599 Size: 32 3399 32 5607 Fragments arranged by size: 5607 3399 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9070 XbaI T'CTAG_A Cuts at: 6406 6406 Size: 9070 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9070 XmaI C'CCGG_G Cuts at: 1870 6395 1870 Size: 4525 4545 XmnI GAAnn'nnTTC Cuts at: 3665 7199 3665 Size: 3534 5536 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6878 276 Size: 53 83 186 936 5344 2468 Fragments arranged by size: 5344 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BstXI BstZ17I EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI XcmI