(Circular) (Six-base) MAPSORT of: pFN21ASDA0959.seq Check: 4811 from: 1 to: 7033 pFN21ASDA0959 7033 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3977 3977 Size: 7033 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3917 4843 278 Size: 53 83 186 936 2381 926 2468 Fragments arranged by size: 2468 2381 936 926 186 83 53 AccI GT'mk_AC Cuts at: 1937 2980 4376 1937 Size: 1043 1396 4594 Fragments arranged by size: 4594 1396 1043 Acc65I G'GTAC_C Cuts at: 4354 4354 Size: 7033 AclI AA'CG_TT Cuts at: 1563 5160 5533 1563 Size: 3597 373 3063 Fragments arranged by size: 3597 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2641 3008 3308 4094 5096 6480 6748 1632 Size: 1009 367 300 786 1002 1384 268 1917 Fragments arranged by size: 1917 1384 1009 1002 786 367 300 268 AfeI AGC'GCT Cuts at: 3899 3899 Size: 7033 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6836 AflIII A'CryG_T Cuts at: 1203 4008 5932 6909 1203 Size: 2805 1924 977 1327 Fragments arranged by size: 2805 1924 1327 977 AgeI A'CCGG_T Cuts at: 5864 5864 Size: 7033 AhdI GACnn_n'nnGTC Cuts at: 2192 5762 2192 Size: 3570 3463 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7033 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7033 AlwNI CAG_nnn'CTG Cuts at: 2533 3478 3527 3719 4776 6348 2533 Size: 945 49 192 1057 1572 3218 Fragments arranged by size: 3218 1572 1057 945 192 49 ApaLI G'TGCA_C Cuts at: 5090 6246 5090 Size: 1156 5877 ApoI r'AATT_y Cuts at: 1493 2702 2992 3176 3374 3820 4342 4568 4720 Size: 1209 290 184 198 446 522 226 152 Cuts at: 4720 6829 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1209 522 446 290 226 198 184 152 AseI AT'TA_AT Cuts at: 160 1032 5587 160 Size: 872 4555 1606 Fragments arranged by size: 4555 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7033 AvaI C'yCGr_G Cuts at: 1870 1943 4358 1870 Size: 73 2415 4545 Fragments arranged by size: 4545 2415 73 BamHI G'GATC_C Cuts at: 4363 4730 4363 Size: 367 6666 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2237 3284 4354 5809 618 Size: 325 217 627 450 1047 1070 1455 1842 Fragments arranged by size: 1842 1455 1070 1047 627 450 325 217 BanII G_rGCy'C Cuts at: 1391 2054 2508 2567 3662 3731 4352 1391 Size: 663 454 59 1095 69 621 4072 Fragments arranged by size: 4072 1095 663 621 454 69 59 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2499 3828 3969 3999 928 Size: 971 600 1329 141 30 3962 Fragments arranged by size: 3962 1329 971 600 141 30 BbvCI CC'TCA_GC Cuts at: 3720 3720 Size: 7033 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3916 3950 5224 5258 1903 Size: 34 1979 34 1274 34 3678 Fragments arranged by size: 3678 1979 1274 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4925 6135 4925 Size: 1210 5823 BclI T'GATC_A Cuts at: 1548 2439 3606 1548 Size: 891 1167 4975 Fragments arranged by size: 4975 1167 891 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5644 136 Size: 107 122 71 631 605 3972 1525 Fragments arranged by size: 3972 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2722 7028 2722 Size: 4306 2727 BlpI GC'TnA_GC Cuts at: 4450 4450 Size: 7033 Bme1580I G_kGCm'C Cuts at: 1790 3276 3287 5094 6250 1790 Size: 1486 11 1807 1156 2573 Fragments arranged by size: 2573 1807 1486 1156 11 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7033 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2074 4764 5722 Size: 416 53 83 399 24 657 2690 958 Cuts at: 5722 442 Size: 1753 Fragments arranged by size: 2690 1753 958 657 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7033 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3745 3802 3745 Size: 57 6976 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2166 2702 3224 4318 5693 1239 Size: 927 536 522 1094 1375 2579 Fragments arranged by size: 2579 1375 1094 927 536 522 Bpu10I CC'TnA_GC Cuts at: 3720 3720 Size: 7033 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2618 3025 5158 6023 6321 2618 Size: 407 2133 865 298 3330 Fragments arranged by size: 3330 2133 865 407 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2811 5696 882 Size: 617 1312 2885 2219 Fragments arranged by size: 2885 2219 1312 617 BsaAI yAC'GTr Cuts at: 493 1204 2709 4330 4824 493 Size: 711 1505 1621 494 2702 Fragments arranged by size: 2702 1621 1505 711 494 BsaBI GATnn'nnATC Cuts at: 2963 7027 2963 Size: 4064 2969 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3531 3914 4840 5222 Size: 53 83 186 936 1998 383 926 382 Cuts at: 5222 5857 275 Size: 635 1451 Fragments arranged by size: 1998 1451 936 926 635 383 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5466 5864 6138 6285 6696 5466 Size: 398 274 147 411 5803 Fragments arranged by size: 5803 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2636 2666 3649 3679 3839 Size: 30 165 30 1233 30 983 30 160 Cuts at: 3839 3869 1178 Size: 30 4342 Fragments arranged by size: 4342 1233 983 165 160 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3500 3503 3737 3775 3847 4118 1186 Size: 2314 3 234 38 72 271 4101 Fragments arranged by size: 4101 2314 271 234 72 38 3 BseYI C'CCAG_C Cuts at: 6236 6732 6236 Size: 496 6537 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2508 4139 1163 Size: 573 772 1631 4057 Fragments arranged by size: 4057 1631 772 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4483 5244 6272 1540 Size: 275 186 2482 761 1028 2301 Fragments arranged by size: 2482 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2054 2587 3662 5094 5179 6250 2054 Size: 533 1075 1432 85 1071 2837 Fragments arranged by size: 2837 1432 1075 1071 533 85 BsmI GAATG_Cn' Cuts at: 2452 3090 4544 4637 2452 Size: 638 1454 93 4848 Fragments arranged by size: 4848 1454 638 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2054 2508 2567 2587 3276 3287 3662 Size: 399 264 454 59 20 689 11 375 Cuts at: 3662 3731 4352 5094 5179 6250 1391 Size: 69 621 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 689 621 454 399 375 264 85 69 59 20 11 BspEI T'CCGG_A Cuts at: 6696 6696 Size: 7033 BspHI T'CATG_A Cuts at: 2971 4922 6652 2971 Size: 1951 1730 3352 Fragments arranged by size: 3352 1951 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2271 3977 4111 4388 844 Size: 1427 1706 134 277 3489 Fragments arranged by size: 3489 1706 1427 277 134 BsrBI CCG'CTC Cuts at: 1394 1823 4920 1394 Size: 429 3097 3507 Fragments arranged by size: 3507 3097 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5528 5702 65 Size: 1183 4280 174 1396 Fragments arranged by size: 4280 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5677 5864 1700 Size: 110 3867 187 2869 Fragments arranged by size: 3867 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4011 96 Size: 3915 3118 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7033 BssSI C'ACGA_G Cuts at: 2726 5093 6105 2726 Size: 2367 1012 3654 Fragments arranged by size: 3654 2367 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7033 BstBI TT'CG_AA Cuts at: 2007 5837 2007 Size: 3830 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7033 BstYI r'GATC_y Cuts at: 1972 2722 4363 4730 5115 5132 6584 6670 6682 Size: 750 1641 367 385 17 1452 86 12 Cuts at: 6682 7028 1972 Size: 346 1977 Fragments arranged by size: 1977 1641 1452 750 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3826 3826 Size: 7033 BtgI C'CryG_G Cuts at: 513 1065 3741 3810 4227 513 Size: 552 2676 69 417 3319 Fragments arranged by size: 3319 2676 552 417 69 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2950 487 Size: 683 56 1724 4570 Fragments arranged by size: 4570 1724 683 56 BtsI GCAGTG_nn' Cuts at: 2785 3023 3202 3315 3645 4549 5343 5363 2785 Size: 238 179 113 330 904 794 20 4455 Fragments arranged by size: 4455 904 794 330 238 179 113 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7033 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6998 DraI TTT'AAA Cuts at: 4337 4337 Size: 7033 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6040 809 Size: 507 4724 1802 Fragments arranged by size: 4724 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4480 5369 6998 Size: 54 1425 50 275 18 2650 889 1629 Cuts at: 6998 8 Size: 43 Fragments arranged by size: 2650 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4480 1537 Size: 275 2668 4090 Fragments arranged by size: 4090 2668 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2067 2575 2661 2667 2768 3554 3566 4963 Size: 714 508 86 6 101 786 12 1397 Cuts at: 4963 1353 Size: 3423 Fragments arranged by size: 3423 1397 786 714 508 101 86 12 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5620 5994 6140 387 Size: 1064 138 4031 374 146 1280 Fragments arranged by size: 4031 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2052 3660 2052 Size: 1608 5425 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2166 2641 2702 3008 3224 3308 4094 Size: 393 534 475 61 306 216 84 786 Cuts at: 4094 4318 5096 5693 6480 6748 1239 Size: 224 778 597 787 268 1524 Fragments arranged by size: 1524 787 786 778 597 534 475 393 306 268 224 216 84 61 EcoNI CCTnn'n_nnAGG Cuts at: 2666 3004 3482 2666 Size: 338 478 6217 Fragments arranged by size: 6217 478 338 EcoO109I rG'GnC_Cy Cuts at: 2469 2469 Size: 7033 EcoRI G'AATT_C Cuts at: 2702 4342 4720 2702 Size: 1640 378 5015 Fragments arranged by size: 5015 1640 378 EcoRV GAT'ATC Cuts at: 3112 3112 Size: 7033 FspI TGC'GCA Cuts at: 4214 5539 4214 Size: 1325 5708 HaeII r_GCGC'y Cuts at: 1678 3901 6180 1678 Size: 2223 2279 2531 Fragments arranged by size: 2531 2279 2223 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2178 2210 2928 2960 3101 Size: 32 135 32 606 32 718 32 141 Cuts at: 3101 3133 3598 3630 5674 5706 5748 5780 1373 Size: 32 465 32 2044 32 42 32 2626 Fragments arranged by size: 2626 2044 718 606 465 141 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4029 4377 4623 669 Size: 988 281 2091 348 246 3079 Fragments arranged by size: 3079 2091 988 348 281 246 HindIII A'AGCT_T Cuts at: 748 2249 748 Size: 1501 5532 HpaI GTT'AAC Cuts at: 4623 4623 Size: 7033 KpnI G_GTAC'C Cuts at: 4358 4358 Size: 7033 MfeI C'AATT_G Cuts at: 4632 4632 Size: 7033 MluI A'CGCG_T Cuts at: 6909 6909 Size: 7033 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6147 6331 1305 Size: 4842 184 2007 Fragments arranged by size: 4842 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5211 Fragments arranged by size: 5211 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2552 3254 3986 4991 5350 5509 518 Size: 648 1386 702 732 1005 359 159 2042 Fragments arranged by size: 2042 1386 1005 732 702 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2176 2740 3524 3654 4052 4449 5126 6274 Size: 570 564 784 130 398 397 677 1148 Cuts at: 6274 6519 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 784 677 570 564 398 397 245 130 NcoI C'CATG_G Cuts at: 513 1065 3741 3810 4227 513 Size: 552 2676 69 417 3319 Fragments arranged by size: 3319 2676 552 417 69 NdeI CA'TA_TG Cuts at: 387 387 Size: 7033 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7033 NotI GC'GGCC_GC Cuts at: 4480 4480 Size: 7033 NspI r_CATG'y Cuts at: 1132 1229 4012 4391 5936 1132 Size: 97 2783 379 1545 2229 Fragments arranged by size: 2783 2229 1545 379 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7033 PciI A'CATG_T Cuts at: 4008 5932 4008 Size: 1924 5109 PflMI CCAn_nnn'nTGG Cuts at: 1108 2791 3811 1108 Size: 1683 1020 4330 Fragments arranged by size: 4330 1683 1020 PfoI T'CCnGG_A Cuts at: 2182 2182 Size: 7033 PmeI GTTT'AAAC Cuts at: 4337 4337 Size: 7033 PmlI CAC'GTG Cuts at: 2709 2709 Size: 7033 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5102 5134 6615 6647 5102 Size: 32 1481 32 5488 Fragments arranged by size: 5488 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7033 PsiI TTA'TAA Cuts at: 4603 4603 Size: 7033 PstI C_TGCA'G Cuts at: 830 3030 3237 4385 830 Size: 2200 207 1148 3478 Fragments arranged by size: 3478 2200 1148 207 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7033 PvuII CAG'CTG Cuts at: 2176 2740 3524 2176 Size: 564 784 5685 Fragments arranged by size: 5685 784 564 SacI G_AGCT'C Cuts at: 2054 3662 2054 Size: 1608 5425 SalI G'TCGA_C Cuts at: 1936 4375 1936 Size: 2439 4594 SapI GCTCTTCn'nnn_ Cuts at: 2575 3554 2575 Size: 979 6054 SbfI CC_TGCA'GG Cuts at: 4385 4385 Size: 7033 ScaI AGT'ACT Cuts at: 5281 6993 5281 Size: 1712 5321 SfcI C'TryA_G Cuts at: 826 1045 3026 3233 4381 5516 6197 6388 826 Size: 219 1981 207 1148 1135 681 191 1471 Fragments arranged by size: 1981 1471 1148 1135 681 219 207 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7033 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7033 SmaI CCC'GGG Cuts at: 1872 4360 1872 Size: 2488 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2597 3004 5137 6038 6300 820 Size: 197 926 654 407 2133 901 262 1553 Fragments arranged by size: 2133 1553 926 901 654 407 262 197 SnaBI TAC'GTA Cuts at: 493 4330 4824 493 Size: 3837 494 2702 Fragments arranged by size: 3837 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7033 SphI G_CATG'C Cuts at: 1132 4391 1132 Size: 3259 3774 SspI AAT'ATT Cuts at: 5 52 4957 5 Size: 47 4905 2081 Fragments arranged by size: 4905 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7033 StyI C'CwwG_G Cuts at: 513 1065 2241 3074 3741 3810 4227 4472 513 Size: 552 1176 833 667 69 417 245 3074 Fragments arranged by size: 3074 1176 833 667 552 417 245 69 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3096 3242 5060 5077 5230 5415 5860 Size: 1255 1516 146 1818 17 153 185 445 Cuts at: 5860 325 Size: 1498 Fragments arranged by size: 1818 1516 1498 1255 445 185 153 146 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4011 5279 6991 Size: 274 80 33 51 1443 2034 1268 1712 Cuts at: 6991 96 Size: 138 Fragments arranged by size: 2034 1712 1443 1268 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3649 3681 6993 7025 3649 Size: 32 3312 32 3657 Fragments arranged by size: 3657 3312 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3680 1316 Size: 2364 4669 XbaI T'CTAG_A Cuts at: 4369 4369 Size: 7033 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3739 3739 Size: 7033 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7033 XmaI C'CCGG_G Cuts at: 1870 4358 1870 Size: 2488 4545 XmnI GAAnn'nnTTC Cuts at: 5162 5162 Size: 7033 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3915 4841 276 Size: 53 83 186 936 2381 926 2468 Fragments arranged by size: 2468 2381 936 926 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I DraIII FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI