Restriction map for
pFN21ASDA0945
(Linear) (Six-base) MAPSORT of: KIAA0945.seq Check: 2802 from: 1 to: 2337
KIAA0945.orf
With 182 enzymes: SgfI *
February 28, 2007 11:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 947 1014 1542 2337
Size: 947 67 528 795
Fragments arranged by size:
947 795 528 67
Acc65I G'GTAC_C
Cuts at: 0 1770 2337
Size: 1770 567
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 392 991 1543 2337
Size: 392 599 552 794
Fragments arranged by size:
794 599 552 392
AflII C'TTAA_G
Cuts at: 0 2323 2337
Size: 2323 14
AflIII A'CryG_T
Cuts at: 0 1716 2337
Size: 1716 621
AleI CACnn'nnGTG
Cuts at: 0 1333 2337
Size: 1333 1004
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 232 1449 2337
Size: 232 1217 888
Fragments arranged by size:
1217 888 232
AlwNI CAG_nnn'CTG
Cuts at: 0 1023 1222 1352 2153 2337
Size: 1023 199 130 801 184
Fragments arranged by size:
1023 801 199 184 130
ApoI r'AATT_y
Cuts at: 0 1456 2337
Size: 1456 881
AvaI C'yCGr_G
Cuts at: 0 124 732 1963 2337
Size: 124 608 1231 374
Fragments arranged by size:
1231 608 374 124
BamHI G'GATC_C
Cuts at: 0 447 2337
Size: 447 1890
BanI G'GyrC_C
Cuts at: 0 149 350 424 986 1273 1511 1770 2337
Size: 149 201 74 562 287 238 259 567
Fragments arranged by size:
567 562 287 259 238 201 149 74
BanII G_rGCy'C
Cuts at: 0 65 133 716 919 1118 1244 1261 1303
Size: 65 68 583 203 199 126 17 42
Cuts at: 1303 1547 1580 2051 2337
Size: 244 33 471 286
Fragments arranged by size:
583 471 286 244 203 199 126 68
65 42 33 17
BbeI G_GCGC'C
Cuts at: 0 153 354 990 2337
Size: 153 201 636 1347
Fragments arranged by size:
1347 636 201 153
BbsI GAAGACnn'nnnn_
Cuts at: 0 83 326 2337
Size: 83 243 2011
Fragments arranged by size:
2011 243 83
BbvCI CC'TCA_GC
Cuts at: 0 1049 2337
Size: 1049 1288
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1245 1279 2337
Size: 1245 34 1058
Fragments arranged by size:
1245 1058 34
BglI GCCn_nnn'nGGC
Cuts at: 0 50 422 744 818 2337
Size: 50 372 322 74 1519
Fragments arranged by size:
1519 372 322 74 50
BglII A'GATC_T
Cuts at: 0 1940 2337
Size: 1940 397
Bme1580I G_kGCm'C
Cuts at: 0 684 1268 1516 1603 2337
Size: 684 584 248 87 734
Fragments arranged by size:
734 684 584 248 87
BmrI ACTGGGnnnn_n'
Cuts at: 0 491 1210 1517 2337
Size: 491 719 307 820
Fragments arranged by size:
820 719 491 307
BmtI G_CTAG'C
Cuts at: 0 617 2337
Size: 617 1720
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 923 2337
Size: 923 1414
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 415 536 931 1352 1638 2337
Size: 250 165 121 395 421 286 699
Fragments arranged by size:
699 421 395 286 250 165 121
Bpu10I CC'TnA_GC
Cuts at: 0 898 1013 1049 1112 2009 2270 2337
Size: 898 115 36 63 897 261 67
Fragments arranged by size:
898 897 261 115 67 63 36
BsaI GGTCTCn'nnnn_
Cuts at: 0 584 2337
Size: 584 1753
BsaAI yAC'GTr
Cuts at: 0 1717 2337
Size: 1717 620
BsaHI Gr'CG_yC
Cuts at: 0 150 351 987 2337
Size: 150 201 636 1350
Fragments arranged by size:
1350 636 201 150
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 486 545 1012 1234 1249 1293 2125 2337
Size: 486 59 467 222 15 44 832 212
Fragments arranged by size:
832 486 467 222 212 59 44 15
BseYI C'CCAG_C
Cuts at: 0 298 335 516 594 717 1482 1621 1766
Size: 298 37 181 78 123 765 139 145
Cuts at: 1766 2035 2188 2337
Size: 269 153 149
Fragments arranged by size:
765 298 269 181 153 149 145 139
123 78 37
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 275 1106 1208 2337
Size: 275 831 102 1129
Fragments arranged by size:
1129 831 275 102
BsiHKAI G_wGCw'C
Cuts at: 0 1118 1261 2015 2337
Size: 1118 143 754 322
Fragments arranged by size:
1118 754 322 143
Bsp1286I G_dGCh'C
Cuts at: 0 65 133 684 716 919 1118 1244 1261
Size: 65 68 551 32 203 199 126 17
Cuts at: 1261 1268 1303 1516 1547 1580 1603 2015 2051
Size: 7 35 213 31 33 23 412 36
Cuts at: 2051 2337
Size: 286
Fragments arranged by size:
551 412 286 213 203 199 126 68
65 36 35 33 32 31 23 17
7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 293 395 947 1014 1542 2138 2337
Size: 293 102 552 67 528 596 199
Fragments arranged by size:
596 552 528 293 199 102 67
BsrBI CCG'CTC
Cuts at: 0 654 1857 2337
Size: 654 1203 480
Fragments arranged by size:
1203 654 480
BsrFI r'CCGG_y
Cuts at: 0 241 2337
Size: 241 2096
BssSI C'ACGA_G
Cuts at: 0 677 1231 2044 2337
Size: 677 554 813 293
Fragments arranged by size:
813 677 554 293
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1023 2337
Size: 1023 1314
BstEII G'GTnAC_C
Cuts at: 0 136 2337
Size: 136 2201
BstXI CCAn_nnnn'nTGG
Cuts at: 0 417 609 821 2337
Size: 417 192 212 1516
Fragments arranged by size:
1516 417 212 192
BstYI r'GATC_y
Cuts at: 0 447 1438 1940 2337
Size: 447 991 502 397
Fragments arranged by size:
991 502 447 397
Bsu36I CC'TnA_GG
Cuts at: 0 1199 1269 2337
Size: 1199 70 1068
Fragments arranged by size:
1199 1068 70
BtgI C'CryG_G
Cuts at: 0 844 2159 2310 2337
Size: 844 1315 151 27
Fragments arranged by size:
1315 844 151 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2057 2337
Size: 2057 280
BtsI GCAGTG_nn'
Cuts at: 0 1118 1171 1667 2337
Size: 1118 53 496 670
Fragments arranged by size:
1118 670 496 53
EaeI y'GGCC_r
Cuts at: 0 51 239 847 1149 1476 1934 2162 2337
Size: 51 188 608 302 327 458 228 175
Fragments arranged by size:
608 458 327 302 228 188 175 51
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2296 2337
Size: 2296 41
EcoICRI GAG'CTC
Cuts at: 0 1116 1259 2337
Size: 1116 143 1078
Fragments arranged by size:
1116 1078 143
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 392 415 536 931 991 1352 1543
Size: 250 142 23 121 395 60 361 191
Cuts at: 1543 1638 2337
Size: 95 699
Fragments arranged by size:
699 395 361 250 191 142 121 95
60 23
EcoNI CCTnn'n_nnAGG
Cuts at: 0 901 2337
Size: 901 1436
EcoO109I rG'GnC_Cy
Cuts at: 0 522 543 574 729 2337
Size: 522 21 31 155 1608
Fragments arranged by size:
1608 522 155 31 21
FspI TGC'GCA
Cuts at: 0 1645 2337
Size: 1645 692
HaeII r_GCGC'y
Cuts at: 0 153 180 354 705 990 1613 1728 2172
Size: 153 27 174 351 285 623 115 444
Cuts at: 2172 2337
Size: 165
Fragments arranged by size:
623 444 351 285 174 165 153 115
27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 26 120 152 688 720 2337
Size: 26 94 32 536 32 1617
Fragments arranged by size:
1617 536 94 32 32 26
KasI G'GCGC_C
Cuts at: 0 149 350 986 2337
Size: 149 201 636 1351
Fragments arranged by size:
1351 636 201 149
KpnI G_GTAC'C
Cuts at: 0 1774 2337
Size: 1774 563
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1433 2337
Size: 1433 904
MscI TGG'CCA
Cuts at: 0 849 1151 1478 1936 2164 2337
Size: 849 302 327 458 228 173
Fragments arranged by size:
849 458 327 302 228 173
MslI CAynn'nnrTG
Cuts at: 0 1333 2297 2337
Size: 1333 964 40
Fragments arranged by size:
1333 964 40
MspA1I CmG'CkG
Cuts at: 0 628 1053 1062 1625 1658 2337
Size: 628 425 9 563 33 679
Fragments arranged by size:
679 628 563 425 33 9
NarI GG'CG_CC
Cuts at: 0 150 351 987 2337
Size: 150 201 636 1350
Fragments arranged by size:
1350 636 201 150
NcoI C'CATG_G
Cuts at: 0 844 2159 2337
Size: 844 1315 178
Fragments arranged by size:
1315 844 178
NheI G'CTAG_C
Cuts at: 0 613 2337
Size: 613 1724
NruI TCG'CGA
Cuts at: 0 2076 2337
Size: 2076 261
NspI r_CATG'y
Cuts at: 0 123 2043 2337
Size: 123 1920 294
Fragments arranged by size:
1920 294 123
PasI CC'CwG_GG
Cuts at: 0 1295 1443 2337
Size: 1295 148 894
Fragments arranged by size:
1295 894 148
PflMI CCAn_nnn'nTGG
Cuts at: 0 779 2337
Size: 779 1558
PfoI T'CCnGG_A
Cuts at: 0 1466 1880 2337
Size: 1466 414 457
Fragments arranged by size:
1466 457 414
PmlI CAC'GTG
Cuts at: 0 1717 2337
Size: 1717 620
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2263 2295 2337
Size: 2263 32 42
Fragments arranged by size:
2263 42 32
PpuMI rG'GwC_Cy
Cuts at: 0 522 543 574 2337
Size: 522 21 31 1763
Fragments arranged by size:
1763 522 31 21
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2134 2166 2337
Size: 2134 32 171
Fragments arranged by size:
2134 171 32
PstI C_TGCA'G
Cuts at: 0 71 810 1166 1438 1539 1663 2149 2223
Size: 71 739 356 272 101 124 486 74
Cuts at: 2223 2337
Size: 114
Fragments arranged by size:
739 486 356 272 124 114 101 74
71
PvuII CAG'CTG
Cuts at: 0 628 1053 1658 2337
Size: 628 425 605 679
Fragments arranged by size:
679 628 605 425
SacI G_AGCT'C
Cuts at: 0 1118 1261 2337
Size: 1118 143 1076
Fragments arranged by size:
1118 1076 143
ScaI AGT'ACT
Cuts at: 0 2143 2337
Size: 2143 194
SfcI C'TryA_G
Cuts at: 0 67 806 1162 1344 1434 1535 1659 2145
Size: 67 739 356 182 90 101 124 486
Cuts at: 2145 2219 2337
Size: 74 118
Fragments arranged by size:
739 486 356 182 124 118 101 90
74 67
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 50 744 2337
Size: 50 694 1593
Fragments arranged by size:
1593 694 50
SfoI GGC'GCC
Cuts at: 0 151 352 988 2337
Size: 151 201 636 1349
Fragments arranged by size:
1349 636 201 151
SmlI C'TyrA_G
Cuts at: 0 2323 2337
Size: 2323 14
SphI G_CATG'C
Cuts at: 0 123 2043 2337
Size: 123 1920 294
Fragments arranged by size:
1920 294 123
StuI AGG'CCT
Cuts at: 0 205 1695 2183 2337
Size: 205 1490 488 154
Fragments arranged by size:
1490 488 205 154
StyI C'CwwG_G
Cuts at: 0 200 525 725 749 773 844 2159 2337
Size: 200 325 200 24 24 71 1315 178
Fragments arranged by size:
1315 325 200 200 178 71 24 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 738 943 1496 1514 1581 2337
Size: 738 205 553 18 67 756
Fragments arranged by size:
756 738 553 205 67 18
TatI w'GTAC_w
Cuts at: 0 1142 2141 2337
Size: 1142 999 196
Fragments arranged by size:
1142 999 196
Tth111I GACn'n_nGTC
Cuts at: 0 1888 2337
Size: 1888 449
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1929 2337
Size: 1929 408
Enzymes that do cut:
AarI Acc65I AcuI AflII AflIII AleI AlfI AlwNI
ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BglI BglII Bme1580I BmrI BmtI BplI BpmI
Bpu10I BsaI BsaAI BsaHI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspMI BsrBI BsrFI BssSI BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI EaeI EciI EcoICRI
Eco57MI EcoNI EcoO109I FspI HaeII Hin4I KasI KpnI
MmeI MscI MslI MspA1I NarI NcoI NheI NruI
NspI PasI PflMI PfoI PmlI PpiI PpuMI PsrI
PstI PvuII SacI ScaI SfcI SfiI SfoI SmlI
SphI StuI StyI TaqII TatI Tth111I XcmI
Enzymes that do not cut:
AatII AccI AclI AfeI AgeI AhdI AloI ApaI
ApaLI AscI AseI AsiSI AvrII BaeI BciVI BclI
BfrBI BlpI BmgBI BpuEI BsaBI BsaWI BsaXI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BssHII
BstBI BstZ17I ClaI CspCI DraI DraIII DrdI EagI
EarI EcoRI EcoRV FalI FseI FspAI HincII HindIII
HpaI MfeI MluI NaeI NdeI NgoMIV NotI NsiI
PacI PciI PmeI PshAI PsiI PspOMI PspXI PvuI
RsrII SacII SalI SanDI SapI SbfI SexAI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI SwaI TstI
XbaI XhoI XmaI XmnI ZraI