(Circular) (Six-base) MAPSORT of: pFN21ASDA0849.seq Check: 8642 from: 1 to: 7588 pFN21ASDA0849 7588 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5398 278 Size: 53 83 186 936 3862 2468 Fragments arranged by size: 3862 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4931 1937 Size: 2994 4594 Acc65I G'GTAC_C Cuts at: 4909 4909 Size: 7588 AclI AA'CG_TT Cuts at: 1563 5715 6088 1563 Size: 4152 373 3063 Fragments arranged by size: 4152 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2449 3462 3550 3617 3641 4065 5651 7035 Size: 817 1013 88 67 24 424 1586 1384 Cuts at: 7035 7303 1632 Size: 268 1917 Fragments arranged by size: 1917 1586 1384 1013 817 424 268 88 67 24 AfeI AGC'GCT Cuts at: 3683 3683 Size: 7588 AflII C'TTAA_G Cuts at: 820 1017 3712 820 Size: 197 2695 4696 Fragments arranged by size: 4696 2695 197 AflIII A'CryG_T Cuts at: 1203 6487 7464 1203 Size: 5284 977 1327 Fragments arranged by size: 5284 1327 977 AgeI A'CCGG_T Cuts at: 6419 6419 Size: 7588 AhdI GACnn_n'nnGTC Cuts at: 6317 6317 Size: 7588 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7588 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2274 4861 1184 Size: 1090 2587 3911 Fragments arranged by size: 3911 2587 1090 AlwNI CAG_nnn'CTG Cuts at: 2678 3050 5331 6903 2678 Size: 372 2281 1572 3363 Fragments arranged by size: 3363 2281 1572 372 ApaLI G'TGCA_C Cuts at: 4815 5645 6801 4815 Size: 830 1156 5602 Fragments arranged by size: 5602 1156 830 ApoI r'AATT_y Cuts at: 1493 2241 2444 4064 4161 4244 4317 4897 5123 Size: 748 203 1620 97 83 73 580 226 Cuts at: 5123 5275 7384 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1620 748 580 226 203 152 97 83 73 AseI AT'TA_AT Cuts at: 160 1032 6142 160 Size: 872 5110 1606 Fragments arranged by size: 5110 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7588 AvaI C'yCGr_G Cuts at: 1870 1943 4913 1870 Size: 73 2970 4545 Fragments arranged by size: 4545 2970 73 BamHI G'GATC_C Cuts at: 4918 5285 4918 Size: 367 7221 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4255 4909 6364 618 Size: 325 217 627 2468 654 1455 1842 Fragments arranged by size: 2468 1842 1455 654 627 325 217 BanII G_rGCy'C Cuts at: 1391 3434 4907 1391 Size: 2043 1473 4072 Fragments arranged by size: 4072 2043 1473 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3087 3165 4365 4782 4828 928 Size: 971 1188 78 1200 417 46 3688 Fragments arranged by size: 3688 1200 1188 971 417 78 46 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5779 5813 1903 Size: 34 3842 34 3678 Fragments arranged by size: 3842 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5480 6690 5480 Size: 1210 6378 BclI T'GATC_A Cuts at: 1548 1548 Size: 7588 BfrBI ATG'CAT Cuts at: 4838 4838 Size: 7588 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6199 136 Size: 107 122 71 631 605 4527 1525 Fragments arranged by size: 4527 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2429 3065 7583 2429 Size: 636 4518 2434 Fragments arranged by size: 4518 2434 636 BlpI GC'TnA_GC Cuts at: 5005 5005 Size: 7588 Bme1580I G_kGCm'C Cuts at: 1790 3595 4819 5649 6805 1790 Size: 1805 1224 830 1156 2573 Fragments arranged by size: 2573 1805 1224 1156 830 BmgBI CAC'GTC Cuts at: 1318 2401 1318 Size: 1083 6505 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2077 2857 3334 Size: 416 53 83 399 24 660 780 477 Cuts at: 3334 4517 4561 5319 6277 442 Size: 1183 44 758 958 1753 Fragments arranged by size: 1753 1183 958 780 758 660 477 416 399 83 53 44 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7588 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2472 2707 3203 4579 4780 6248 1239 Size: 1233 235 496 1376 201 1468 2579 Fragments arranged by size: 2579 1468 1376 1233 496 235 201 Bpu10I CC'TnA_GC Cuts at: 2803 2803 Size: 7588 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4464 4710 5713 6578 6876 4464 Size: 246 1003 865 298 5176 Fragments arranged by size: 5176 1003 865 298 246 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6251 882 Size: 617 4752 2219 Fragments arranged by size: 4752 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4885 5379 493 Size: 711 3681 494 2702 Fragments arranged by size: 3681 2702 711 494 BsaBI GATnn'nnATC Cuts at: 7582 7582 Size: 7588 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5395 5777 6412 275 Size: 53 83 186 936 3862 382 635 1451 Fragments arranged by size: 3862 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2498 4429 6021 6419 6693 6840 7251 2498 Size: 1931 1592 398 274 147 411 2835 Fragments arranged by size: 2835 1931 1592 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2118 2148 3201 3231 1178 Size: 30 165 30 715 30 1053 30 5535 Fragments arranged by size: 5535 1053 715 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2357 3216 1186 Size: 1171 859 5558 Fragments arranged by size: 5558 1171 859 BseYI C'CCAG_C Cuts at: 6791 7287 6791 Size: 496 7092 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2891 3351 3564 1163 Size: 573 1155 460 213 5187 Fragments arranged by size: 5187 1155 573 460 213 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4693 5038 5799 6827 1540 Size: 275 186 2692 345 761 1028 2301 Fragments arranged by size: 2692 2301 1028 761 345 275 186 BsiHKAI G_wGCw'C Cuts at: 3516 4819 5649 5734 6805 3516 Size: 1303 830 85 1071 4299 Fragments arranged by size: 4299 1303 1071 830 85 BsmI GAATG_Cn' Cuts at: 2108 2191 4421 4783 5099 5192 2108 Size: 83 2230 362 316 93 4504 Fragments arranged by size: 4504 2230 362 316 93 83 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3434 3516 3595 4819 4907 5649 5734 Size: 399 1644 82 79 1224 88 742 85 Cuts at: 5734 6805 1391 Size: 1071 2174 Fragments arranged by size: 2174 1644 1224 1071 742 399 88 85 82 79 BspEI T'CCGG_A Cuts at: 2498 4429 7251 2498 Size: 1931 2822 2835 Fragments arranged by size: 2835 2822 1931 BspHI T'CATG_A Cuts at: 5477 7207 5477 Size: 1730 5858 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2663 2683 4118 4478 4788 4943 844 Size: 1819 20 1435 360 310 155 3489 Fragments arranged by size: 3489 1819 1435 360 310 155 20 BsrBI CCG'CTC Cuts at: 1394 1823 2077 5475 1394 Size: 429 254 3398 3507 Fragments arranged by size: 3507 3398 429 254 BsrDI GCAATG_nn' Cuts at: 65 1248 3653 4404 6083 6257 65 Size: 1183 2405 751 1679 174 1396 Fragments arranged by size: 2405 1679 1396 1183 751 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3661 6232 6419 1700 Size: 110 1851 2571 187 2869 Fragments arranged by size: 2869 2571 1851 187 110 BsrGI T'GTAC_A Cuts at: 96 4875 96 Size: 4779 2809 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7588 BssSI C'ACGA_G Cuts at: 5648 6660 5648 Size: 1012 6576 BstAPI GCAn_nnn'nTGC Cuts at: 794 4578 794 Size: 3784 3804 BstBI TT'CG_AA Cuts at: 2007 6392 2007 Size: 4385 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7588 BstYI r'GATC_y Cuts at: 1972 2429 3035 3065 4054 4918 5285 5670 5687 Size: 457 606 30 989 864 367 385 17 Cuts at: 5687 7139 7225 7237 7583 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 989 864 606 457 385 367 346 86 30 17 12 Bsu36I CC'TnA_GG Cuts at: 4059 4059 Size: 7588 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 7036 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6849 Fragments arranged by size: 6849 683 56 BtsI GCAGTG_nn' Cuts at: 2649 3363 5104 5898 5918 2649 Size: 714 1741 794 20 4319 Fragments arranged by size: 4319 1741 794 714 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7588 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7553 DraI TTT'AAA Cuts at: 2362 4303 4312 4892 2362 Size: 1941 9 580 5058 Fragments arranged by size: 5058 1941 580 9 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6595 809 Size: 507 5279 1802 Fragments arranged by size: 5279 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3313 3393 4686 Size: 54 1425 50 275 18 1483 80 1293 Cuts at: 4686 5035 5924 7553 8 Size: 349 889 1629 43 Fragments arranged by size: 1629 1483 1425 1293 889 349 275 80 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5035 1537 Size: 275 3223 4090 Fragments arranged by size: 4090 3223 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2067 3348 3617 4493 4676 5518 1353 Size: 714 1281 269 876 183 842 3423 Fragments arranged by size: 3423 1281 876 842 714 269 183 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6175 6549 6695 387 Size: 1064 138 4586 374 146 1280 Fragments arranged by size: 4586 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2449 2472 2707 3203 3462 3550 3617 Size: 393 817 23 235 496 259 88 67 Cuts at: 3617 3641 4065 4579 4780 5651 6248 7035 7303 Size: 24 424 514 201 871 597 787 268 Cuts at: 7303 1239 Size: 1524 Fragments arranged by size: 1524 871 817 787 597 514 496 424 393 268 259 235 201 88 67 24 23 EcoNI CCTnn'n_nnAGG Cuts at: 3599 3599 Size: 7588 EcoO109I rG'GnC_Cy Cuts at: 2471 2675 2471 Size: 204 7384 EcoRI G'AATT_C Cuts at: 4064 4897 5275 4064 Size: 833 378 6377 Fragments arranged by size: 6377 833 378 EcoRV GAT'ATC Cuts at: 2926 3186 2926 Size: 260 7328 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2220 4505 2220 Size: 2285 5303 FspI TGC'GCA Cuts at: 6094 6094 Size: 7588 HaeII r_GCGC'y Cuts at: 1678 3608 3685 6735 1678 Size: 1930 77 3050 2531 Fragments arranged by size: 3050 2531 1930 77 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4013 4045 4244 4276 4492 Size: 32 135 32 2441 32 199 32 216 Cuts at: 4492 4524 6229 6261 6303 6335 1373 Size: 32 1705 32 42 32 2626 Fragments arranged by size: 2626 2441 1705 216 199 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3103 4932 5178 669 Size: 988 281 1165 1829 246 3079 Fragments arranged by size: 3079 1829 1165 988 281 246 HindIII A'AGCT_T Cuts at: 748 2800 748 Size: 2052 5536 HpaI GTT'AAC Cuts at: 5178 5178 Size: 7588 KpnI G_GTAC'C Cuts at: 4913 4913 Size: 7588 MfeI C'AATT_G Cuts at: 2335 5187 2335 Size: 2852 4736 MluI A'CGCG_T Cuts at: 7464 7464 Size: 7588 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2722 3461 4886 6702 6886 1305 Size: 1417 739 1425 1816 184 2007 Fragments arranged by size: 2007 1816 1425 1417 739 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3315 3395 10 Size: 54 1425 343 1483 80 4203 Fragments arranged by size: 4203 1483 1425 343 80 54 MslI CAynn'nnrTG Cuts at: 518 1166 2919 3287 4261 4843 5546 5905 6064 Size: 648 1753 368 974 582 703 359 159 Cuts at: 6064 518 Size: 2042 Fragments arranged by size: 2042 1753 974 703 648 582 368 359 159 MspA1I CmG'CkG Cuts at: 1606 2425 4442 5004 5681 6829 7074 1606 Size: 819 2017 562 677 1148 245 2120 Fragments arranged by size: 2120 2017 1148 819 677 562 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7036 NdeI CA'TA_TG Cuts at: 387 387 Size: 7588 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7588 NotI GC'GGCC_GC Cuts at: 5035 5035 Size: 7588 NsiI A_TGCA'T Cuts at: 4840 4840 Size: 7588 NspI r_CATG'y Cuts at: 1132 1229 4852 4946 6491 1132 Size: 97 3623 94 1545 2229 Fragments arranged by size: 3623 2229 1545 97 94 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7588 PciI A'CATG_T Cuts at: 6487 6487 Size: 7588 PflMI CCAn_nnn'nTGG Cuts at: 1108 4588 1108 Size: 3480 4108 PfoI T'CCnGG_A Cuts at: 2449 4796 2449 Size: 2347 5241 PmeI GTTT'AAAC Cuts at: 4892 4892 Size: 7588 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2333 2365 5657 5689 7170 7202 2333 Size: 32 3292 32 1481 32 2719 Fragments arranged by size: 3292 2719 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2471 2675 2471 Size: 204 7384 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7588 PsiI TTA'TAA Cuts at: 5158 5158 Size: 7588 PstI C_TGCA'G Cuts at: 830 3632 4940 830 Size: 2802 1308 3478 Fragments arranged by size: 3478 2802 1308 PvuI CG_AT'CG Cuts at: 2001 4693 2001 Size: 2692 4896 PvuII CAG'CTG Cuts at: 2425 4442 2425 Size: 2017 5571 SalI G'TCGA_C Cuts at: 1936 4930 1936 Size: 2994 4594 SapI GCTCTTCn'nnn_ Cuts at: 2067 3348 3617 4676 2067 Size: 1281 269 1059 4979 Fragments arranged by size: 4979 1281 1059 269 SbfI CC_TGCA'GG Cuts at: 4940 4940 Size: 7588 ScaI AGT'ACT Cuts at: 4757 5836 7548 4757 Size: 1079 1712 4797 Fragments arranged by size: 4797 1712 1079 SfcI C'TryA_G Cuts at: 826 1045 3030 3189 3628 4936 6071 6752 6943 Size: 219 1985 159 439 1308 1135 681 191 Cuts at: 6943 826 Size: 1471 Fragments arranged by size: 1985 1471 1308 1135 681 439 219 191 159 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7588 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7588 SmaI CCC'GGG Cuts at: 1872 4915 1872 Size: 3043 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3712 4479 4725 5692 6593 6855 Size: 197 926 1769 767 246 967 901 262 Cuts at: 6855 820 Size: 1553 Fragments arranged by size: 1769 1553 967 926 901 767 262 246 197 SnaBI TAC'GTA Cuts at: 493 4885 5379 493 Size: 4392 494 2702 Fragments arranged by size: 4392 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7588 SphI G_CATG'C Cuts at: 1132 4946 1132 Size: 3814 3774 SspI AAT'ATT Cuts at: 5 52 2505 3908 4075 5512 5 Size: 47 2453 1403 167 1437 2081 Fragments arranged by size: 2453 2081 1437 1403 167 47 StuI AGG'CCT Cuts at: 1517 2388 2959 3634 1517 Size: 871 571 675 5471 Fragments arranged by size: 5471 871 675 571 StyI C'CwwG_G Cuts at: 513 1065 2566 3390 4788 4807 5027 513 Size: 552 1501 824 1398 19 220 3074 Fragments arranged by size: 3074 1501 1398 824 552 220 19 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3476 5615 5632 5785 5970 6415 325 Size: 1255 1896 2139 17 153 185 445 1498 Fragments arranged by size: 2139 1896 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2419 2900 4755 Size: 274 80 33 51 1443 442 481 1855 Cuts at: 4755 4875 5834 7546 96 Size: 120 959 1712 138 Fragments arranged by size: 1855 1712 1443 959 481 442 274 138 120 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2118 2150 3201 3233 3524 3556 7548 7580 2118 Size: 32 1051 32 291 32 3992 32 2126 Fragments arranged by size: 3992 2126 1051 291 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7588 XbaI T'CTAG_A Cuts at: 2245 3421 4924 2245 Size: 1176 1503 4909 Fragments arranged by size: 4909 1503 1176 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7588 XmaI C'CCGG_G Cuts at: 1870 4913 1870 Size: 3043 4545 XmnI GAAnn'nnTTC Cuts at: 2445 3257 4712 5717 2445 Size: 812 1455 1005 4316 Fragments arranged by size: 4316 1455 1005 812 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5396 276 Size: 53 83 186 936 3862 2468 Fragments arranged by size: 3862 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI