Restriction map for
pFN21ASDA0842
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# NO CUT ENZYMES ( 68 enzymes) #
##################################
Acc65I AclI AflII AgeI AscI
AseI AsnI Asp718I AviII AvrII
BbeI BfrI BglII BlnI BsaBI
BsiWI Bsp1407I BspDI BspLU11I BsrDI
BsrGI BssHII Bst1107I BstBI BstZ17I
ClaI DraI EcoRI EcoRV EcoT22I
FseI FspI HincII HindII HindIII
HpaI KasI KpnI MamI MfeI
MluI MseI MunI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PinAI PmeI PshAI PshBI
Psp1406I PvuI SalI SfoI SfuI
SgrAI SnaBI SpeI SspBI SspI
SwaI Tru9I XbaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AatII 2434 GACGTC GACGT^C
AccII 3028 CGCG CG^CG
AflIII 1796 ACGTGT A^C[GA][CT]GT
AocI 1489 CCTGAGG CC^T[ACGT]AGG
ApaI 2274 GGGCCC GGGCC^C
BbvCI 1693 GCTGAGG CCTCAGC(-5/-2)
BciVI 1554 GGATAC GTATCC(6/5)
BclI 2105 TGATCA T^GATCA
BsmBI 2769 CGTCTC CGTCTC(1/5)
BsmI 378 GAATGC GAATGC(1/-1)
BspHI 1787 TCATGA T^CATGA
BstEII 942 GGTCACC G^GT[ACGT]ACC
BstPI 942 GGTCACC G^GT[ACGT]ACC
BstUI 3028 CGCG CG^CG
Bsu36I 1489 CCTGAGG CC^T[ACGT]AGG
DraIII 1272 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 3030 CGGCCG C^GGCCG
EclXI 3030 CGGCCG C^GGCCG
Eco52I 3030 CGGCCG C^GGCCG
Eco81I 1489 CCTGAGG CC^T[ACGT]AGG
EcoO65I 942 GGTCACC G^GT[ACGT]ACC
FbaI 2105 TGATCA T^GATCA
KspI 3027 CCGCGG CCGC^GG
MvnI 3028 CGCG CG^CG
NaeI 825 GCCGGC GCC^GGC
NgoMIV 825 GCCGGC G^CCGGC
PaeR7I 928 CTCGAG C^TCGAG
RcaI 1787 TCATGA T^CATGA
SacI 925 GAGCTC GAGCT^C
SacII 3027 CCGCGG CCGC^GG
SbfI 1450 CCTGCAGG CCTGCA^GG
SfiI 2574 GGCCGAGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1324 CCCGGG CCC^GGG
SphI 2327 GCATGC GCATG^C
Sse8387I 1450 CCTGCAGG CCTGCA^GG
StuI 776 AGGCCT AGG^CCT
XhoI 928 CTCGAG C^TCGAG
XmaCI 1324 CCCGGG C^CCGGG
XmaI 1324 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 34 enzymes) #
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AccI 1762 GTAGAC GT^[AC][GT]AC
1903 GTCTAC
AcsI 2179 AAATTT [GA]^AATT[CT]
2968 AAATTC
AcyI 1536 GACGCC G[GA]^CG[CT]C
2434 GACGTC
AhdI 568 GACAGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1975 GACTATGTGTC
ApoI 2179 AAATTT [GA]^AATT[CT]
2968 AAATTC
Asp700I 807 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2964 GAAGAAATTC
AspEI 568 GACAGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1975 GACTATGTGTC
BamHI 26 GGATCC G^GATCC
2405 GGATCC
BanI 2308 GGCACC G^G[CT][GA]CC
2347 GGCACC
BbrPI 253 CACGTG CAC^GTG
1795 CACGTG
BbsI 535 GAAGAC GAAGAC(2/6)
2376 GAAGAC
BglI 1058 GCCGCAACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2575 GCCGAGATGGC
BlpI 1288 GCTGAGC GC^T[ACGT]AGC
2394 GCTCAGC
Bpu1102I 1288 GCTGAGC GC^T[ACGT]AGC
2394 GCTCAGC
BpuAI 535 GAAGAC GAAGAC(2/6)
2376 GAAGAC
BsaHI 1536 GACGCC G[GA]^CG[CT]C
2434 GACGTC
BsiEI 2538 CGACCG CG[GA][CT]^CG
3030 CGGCCG
BsrBI 744 CCGCTC CCGCTC(-3/-3)
2730 CCGCTC
BssSI 1560 CACGAG CACGAG(-5/-1)
199 CTCGTG
CelII 1288 GCTGAGC GC^T[ACGT]AGC
2394 GCTCAGC
CpoI 1312 CGGACCG CG^G[AT]CCG
2423 CGGACCG
DrdI 420 GACCTTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1894 GACTGCTATGTC
Eam1105I 568 GACAGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1975 GACTATGTGTC
Hin1I 1536 GACGCC G[GA]^CG[CT]C
2434 GACGTC
MslI 855 CACCCCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1200 CACCTCCATG
NcoI 1205 CCATGG C^CATGG
2156 CCATGG
PmaCI 253 CACGTG CAC^GTG
1795 CACGTG
PmlI 253 CACGTG CAC^GTG
1795 CACGTG
RsrII 1312 CGGACCG CG^G[AT]CCG
2423 CGGACCG
SapI 1813 GAAGAGC GCTCTTC(1/4)
411 GCTCTTC
ScaI 365 AGTACT AGT^ACT
2792 AGTACT
SexAI 1273 ACCTGGT A^CC[AT]GGT
1940 ACCTGGT
TfiI 1699 GATTC G^A[AT]TC
2194 GAATC
XmnI 807 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2964 GAAGAAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccIII AciI AfaI AluI Alw44I
AlwI AlwNI Aor51HI ApaLI AspHI
AspI AvaI AvaII BalI BanII
BbvI BcgI BcnI BfaI BmyI
BpmI BsaAI BsaI BsaJI BsaWI
BseAI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspEI BspMI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco47III EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MroI MscI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflFI PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XcmI XhoII