(Circular) (Six-base) MAPSORT of: pFN21ASDA0842.seq Check: 1135 from: 1 to: 7786 pFN21ASDA0842 7786 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3807 3807 Size: 7786 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4461 5596 278 Size: 53 83 186 936 2925 1135 2468 Fragments arranged by size: 2925 2468 1135 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3786 3927 5129 1937 Size: 1849 141 1202 4594 Fragments arranged by size: 4594 1849 1202 141 Acc65I G'GTAC_C Cuts at: 5107 5107 Size: 7786 AclI AA'CG_TT Cuts at: 1563 5913 6286 1563 Size: 4350 373 3063 Fragments arranged by size: 4350 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 5849 7233 7501 1632 Size: 4217 1384 268 1917 Fragments arranged by size: 4217 1917 1384 268 AfeI AGC'GCT Cuts at: 3185 3236 3715 3185 Size: 51 479 7256 Fragments arranged by size: 7256 479 51 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7589 AflIII A'CryG_T Cuts at: 1203 3819 6685 7662 1203 Size: 2616 2866 977 1327 Fragments arranged by size: 2866 2616 1327 977 AgeI A'CCGG_T Cuts at: 6617 6617 Size: 7786 AhdI GACnn_n'nnGTC Cuts at: 2596 4003 6515 2596 Size: 1407 2512 3867 Fragments arranged by size: 3867 2512 1407 AleI CACnn'nnGTG Cuts at: 1166 2882 1166 Size: 1716 6070 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7786 AlwNI CAG_nnn'CTG Cuts at: 2155 2545 2858 2923 3250 3297 3601 3653 3898 Size: 390 313 65 327 47 304 52 245 Cuts at: 3898 4066 4677 5025 5046 5529 7101 2155 Size: 168 611 348 21 483 1572 2840 Fragments arranged by size: 2840 1572 611 483 390 348 327 313 304 245 168 65 52 47 21 ApaI G_GGCC'C Cuts at: 4301 4301 Size: 7786 ApaLI G'TGCA_C Cuts at: 2885 4042 4265 5843 6999 2885 Size: 1157 223 1578 1156 3672 Fragments arranged by size: 3672 1578 1157 1156 223 ApoI r'AATT_y Cuts at: 1493 4202 4991 5095 5321 5473 7582 1493 Size: 2709 789 104 226 152 2109 1697 Fragments arranged by size: 2709 2109 1697 789 226 152 104 AseI AT'TA_AT Cuts at: 160 1032 6340 160 Size: 872 5308 1606 Fragments arranged by size: 5308 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7786 AvaI C'yCGr_G Cuts at: 1870 1943 2951 3347 4325 5064 5111 1870 Size: 73 1008 396 978 739 47 4545 Fragments arranged by size: 4545 1008 978 739 396 73 47 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2525 2558 2525 Size: 33 7753 BamHI G'GATC_C Cuts at: 2049 4428 5116 5483 2049 Size: 2379 688 367 4352 Fragments arranged by size: 4352 2379 688 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4331 4370 5107 6562 618 Size: 325 217 627 2544 39 737 1455 1842 Fragments arranged by size: 2544 1842 1455 737 627 325 217 39 BanII G_rGCy'C Cuts at: 1391 2816 2952 3318 3752 4301 5105 1391 Size: 1425 136 366 434 549 804 4072 Fragments arranged by size: 4072 1425 804 549 434 366 136 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2565 4406 928 Size: 971 666 1841 4308 Fragments arranged by size: 4308 1841 971 666 BbvCI CC'TCA_GC Cuts at: 3717 3717 Size: 7786 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2219 2253 2932 2966 3244 3278 5977 Size: 34 282 34 679 34 278 34 2699 Cuts at: 5977 6011 1903 Size: 34 3678 Fragments arranged by size: 3678 2699 679 282 278 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3571 5678 6888 3571 Size: 2107 1210 4469 Fragments arranged by size: 4469 2107 1210 BclI T'GATC_A Cuts at: 1548 4128 1548 Size: 2580 5206 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3087 4604 6397 Size: 107 122 71 631 605 1415 1517 1793 Cuts at: 6397 136 Size: 1525 Fragments arranged by size: 1793 1525 1517 1415 631 605 122 107 71 BglII A'GATC_T Cuts at: 7781 7781 Size: 7786 BlpI GC'TnA_GC Cuts at: 3312 4418 5203 3312 Size: 1106 785 5895 Fragments arranged by size: 5895 1106 785 Bme1580I G_kGCm'C Cuts at: 1790 2889 3876 4046 4269 4301 4334 4373 4766 Size: 1099 987 170 223 32 33 39 393 Cuts at: 4766 5847 7003 1790 Size: 1081 1156 2573 Fragments arranged by size: 2573 1156 1099 1081 987 393 223 170 39 33 32 BmgBI CAC'GTC Cuts at: 1318 4404 1318 Size: 3086 4700 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2224 2737 4279 Size: 416 53 83 399 24 807 513 1542 Cuts at: 4279 5517 6475 442 Size: 1238 958 1753 Fragments arranged by size: 1753 1542 1238 958 807 513 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7786 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2080 3609 2080 Size: 1529 6257 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2075 2657 3623 4595 6446 1239 Size: 836 582 966 972 1851 2579 Fragments arranged by size: 2579 1851 972 966 836 582 Bpu10I CC'TnA_GC Cuts at: 3251 3594 3717 4877 3251 Size: 343 123 1160 6160 Fragments arranged by size: 6160 1160 343 123 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3665 5026 5911 6776 7074 3665 Size: 1361 885 865 298 4377 Fragments arranged by size: 4377 1361 885 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2733 3681 5061 6449 882 Size: 617 1234 948 1380 1388 2219 Fragments arranged by size: 2219 1388 1380 1234 948 617 BsaAI yAC'GTr Cuts at: 493 1204 2278 3820 3904 4722 5083 5577 493 Size: 711 1074 1542 84 818 361 494 2702 Fragments arranged by size: 2702 1542 1074 818 711 494 361 84 BsaBI GATnn'nnATC Cuts at: 7780 7780 Size: 7786 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3560 4458 5593 5975 Size: 53 83 186 936 2027 898 1135 382 Cuts at: 5975 6610 275 Size: 635 1451 Fragments arranged by size: 2027 1451 1135 936 898 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2046 2127 3858 3936 6219 6617 6891 7038 7449 Size: 81 1731 78 2283 398 274 147 411 Cuts at: 7449 2046 Size: 2383 Fragments arranged by size: 2383 2283 1731 411 398 274 147 81 78 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2318 2348 3328 3358 5026 Size: 30 165 30 915 30 980 30 1668 Cuts at: 5026 5056 1178 Size: 30 3908 Fragments arranged by size: 3908 1668 980 915 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2194 2857 2858 2924 3029 3158 3555 3558 Size: 1008 663 1 66 105 129 397 3 Cuts at: 3558 3561 4844 1186 Size: 3 1283 4128 Fragments arranged by size: 4128 1283 1008 663 397 129 105 66 3 3 1 BseYI C'CCAG_C Cuts at: 2570 2717 2820 3290 3609 3695 4157 4670 4774 Size: 147 103 470 319 86 462 513 104 Cuts at: 4774 6989 7485 2570 Size: 2215 496 2871 Fragments arranged by size: 2871 2215 513 496 470 462 319 147 104 103 86 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2926 4340 4867 4896 1163 Size: 573 1190 1414 527 29 4053 Fragments arranged by size: 4053 1414 1190 573 527 29 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4564 5056 5236 5997 7025 1540 Size: 275 186 2563 492 180 761 1028 2301 Fragments arranged by size: 2563 2301 1028 761 492 275 186 180 BsiHKAI G_wGCw'C Cuts at: 2166 2223 2889 2952 3305 3869 4046 4269 4419 Size: 57 666 63 353 564 177 223 150 Cuts at: 4419 5847 5932 7003 2166 Size: 1428 85 1071 2949 Fragments arranged by size: 2949 1428 1071 666 564 353 223 177 150 85 63 57 BsmI GAATG_Cn' Cuts at: 2407 5297 5390 2407 Size: 2890 93 4803 Fragments arranged by size: 4803 2890 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4798 4798 Size: 7786 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2166 2223 2816 2889 2952 3305 3318 Size: 399 376 57 593 73 63 353 13 Cuts at: 3318 3752 3869 3876 4046 4269 4301 4334 4373 Size: 434 117 7 170 223 32 33 39 Cuts at: 4373 4419 4766 5105 5847 5932 7003 1391 Size: 46 347 339 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 593 434 399 376 353 347 339 223 170 117 85 73 63 57 46 39 33 32 13 7 BspEI T'CCGG_A Cuts at: 2127 3858 3936 7449 2127 Size: 1731 78 3513 2464 Fragments arranged by size: 3513 2464 1731 78 BspHI T'CATG_A Cuts at: 3810 5675 7405 3810 Size: 1865 1730 4191 Fragments arranged by size: 4191 1865 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2358 2553 3807 3897 3939 5141 844 Size: 1514 195 1254 90 42 1202 3489 Fragments arranged by size: 3489 1514 1254 1202 195 90 42 BsrBI CCG'CTC Cuts at: 1394 1823 2769 4755 5673 1394 Size: 429 946 1986 918 3507 Fragments arranged by size: 3507 1986 946 918 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6281 6455 65 Size: 1183 5033 174 1396 Fragments arranged by size: 5033 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2848 3338 4563 6430 6617 1700 Size: 110 1038 490 1225 1867 187 2869 Fragments arranged by size: 2869 1867 1225 1038 490 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7786 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7786 BssSI C'ACGA_G Cuts at: 2222 3583 5846 6858 2222 Size: 1361 2263 1012 3150 Fragments arranged by size: 3150 2263 1361 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3300 4066 4686 794 Size: 2506 766 620 3894 Fragments arranged by size: 3894 2506 766 620 BstBI TT'CG_AA Cuts at: 2007 6590 2007 Size: 4583 3203 BstEII G'GTnAC_C Cuts at: 1184 2965 1184 Size: 1781 6005 BstXI CCAn_nnnn'nTGG Cuts at: 2290 2635 4207 2290 Size: 345 1572 5869 Fragments arranged by size: 5869 1572 345 BstYI r'GATC_y Cuts at: 1972 2049 2115 2482 4428 5116 5483 5868 5885 Size: 77 66 367 1946 688 367 385 17 Cuts at: 5885 7337 7423 7435 7781 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1946 1452 688 385 367 367 346 86 77 66 17 12 Bsu36I CC'TnA_GG Cuts at: 3513 3513 Size: 7786 BtgI C'CryG_G Cuts at: 513 1065 2022 2581 3168 3228 4179 4524 5050 Size: 552 957 559 587 60 951 345 526 Cuts at: 5050 513 Size: 3249 Fragments arranged by size: 3249 957 951 587 559 552 526 345 60 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7047 Fragments arranged by size: 7047 683 56 BtsI GCAGTG_nn' Cuts at: 2709 4312 4502 5302 6096 6116 2709 Size: 1603 190 800 794 20 4379 Fragments arranged by size: 4379 1603 800 794 190 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7786 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7751 DraI TTT'AAA Cuts at: 5090 5090 Size: 7786 DraIII CAC_nnn'GTG Cuts at: 3300 3300 Size: 7786 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2449 3923 6793 809 Size: 507 1133 1474 2870 1802 Fragments arranged by size: 2870 1802 1474 1133 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2280 3460 3484 Size: 54 1425 50 275 18 450 1180 24 Cuts at: 3484 3523 4197 4605 5053 5233 6122 7751 8 Size: 39 674 408 448 180 889 1629 43 Fragments arranged by size: 1629 1425 1180 889 674 450 448 408 275 180 54 50 43 39 24 18 EagI C'GGCC_G Cuts at: 1537 1812 5053 5233 1537 Size: 275 3241 180 4090 Fragments arranged by size: 4090 3241 275 180 EarI CTCTTCn'nnn_ Cuts at: 1353 2441 3528 3831 3966 5716 1353 Size: 1088 1087 303 135 1750 3423 Fragments arranged by size: 3423 1750 1088 1087 303 135 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6373 6747 6893 387 Size: 1064 138 4784 374 146 1280 Fragments arranged by size: 4784 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2950 2950 Size: 7786 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2075 2657 3623 4595 5849 6446 7233 Size: 393 443 582 966 972 1254 597 787 Cuts at: 7233 7501 1239 Size: 268 1524 Fragments arranged by size: 1524 1254 972 966 787 597 582 443 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2806 3254 4385 2806 Size: 448 1131 6207 Fragments arranged by size: 6207 1131 448 EcoO109I rG'GnC_Cy Cuts at: 2143 2920 3632 3657 4276 4298 2143 Size: 777 712 25 619 22 5631 Fragments arranged by size: 5631 777 712 619 25 22 EcoRI G'AATT_C Cuts at: 5095 5473 5095 Size: 378 7408 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2582 4423 2582 Size: 1841 5945 FspI TGC'GCA Cuts at: 6292 6292 Size: 7786 HaeII r_GCGC'y Cuts at: 1678 2827 3187 3238 3717 4091 4791 5025 6933 Size: 1149 360 51 479 374 700 234 1908 Cuts at: 6933 1678 Size: 2531 Fragments arranged by size: 2531 1908 1149 700 479 374 360 234 51 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2189 2221 2235 2267 2582 Size: 32 135 32 617 32 14 32 315 Cuts at: 2582 2614 2846 2878 3328 3360 3989 4021 4606 Size: 32 232 32 450 32 629 32 585 Cuts at: 4606 4638 5026 5058 6427 6459 6501 6533 1373 Size: 32 388 32 1369 32 42 32 2626 Fragments arranged by size: 2626 1369 629 617 585 450 388 315 232 135 42 32 32 32 32 32 32 32 32 32 32 32 32 14 HincII GTy'rAC Cuts at: 669 1657 1938 5130 5376 669 Size: 988 281 3192 246 3079 Fragments arranged by size: 3192 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7786 HpaI GTT'AAC Cuts at: 5376 5376 Size: 7786 KpnI G_GTAC'C Cuts at: 5111 5111 Size: 7786 MfeI C'AATT_G Cuts at: 5385 5385 Size: 7786 MluI A'CGCG_T Cuts at: 7662 7662 Size: 7786 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2805 4584 5004 6900 7084 1305 Size: 1500 1779 420 1896 184 2007 Fragments arranged by size: 2007 1896 1779 1500 420 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2282 3486 3525 4199 10 Size: 54 1425 343 450 1204 39 674 3597 Fragments arranged by size: 3597 1425 1204 674 450 343 54 39 MslI CAynn'nnrTG Cuts at: 518 1166 2882 3227 5744 6103 6262 518 Size: 648 1716 345 2517 359 159 2042 Fragments arranged by size: 2517 2042 1716 648 359 345 159 MspA1I CmG'CkG Cuts at: 1606 3283 3493 3613 3844 3895 5032 5052 5202 Size: 1677 210 120 231 51 1137 20 150 Cuts at: 5202 5879 7027 7272 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1677 1148 1137 677 245 231 210 150 120 51 20 NaeI GCC'GGC Cuts at: 2850 2850 Size: 7786 NcoI C'CATG_G Cuts at: 513 1065 2022 3228 4179 513 Size: 552 957 1206 951 4120 Fragments arranged by size: 4120 1206 957 951 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7786 NgoMIV G'CCGG_C Cuts at: 2848 2848 Size: 7786 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7786 NotI GC'GGCC_GC Cuts at: 5233 5233 Size: 7786 NspI r_CATG'y Cuts at: 1132 1229 2106 2521 3500 4354 5144 6689 1132 Size: 97 877 415 979 854 790 1545 2229 Fragments arranged by size: 2229 1545 979 877 854 790 415 97 PasI CC'CwG_GG Cuts at: 1348 4294 4657 4863 1348 Size: 2946 363 206 4271 Fragments arranged by size: 4271 2946 363 206 PciI A'CATG_T Cuts at: 6685 6685 Size: 7786 PflMI CCAn_nnn'nTGG Cuts at: 1108 2923 3101 3297 3653 1108 Size: 1815 178 196 356 5241 Fragments arranged by size: 5241 1815 356 196 178 PfoI T'CCnGG_A Cuts at: 2052 2361 4968 2052 Size: 309 2607 4870 Fragments arranged by size: 4870 2607 309 PmeI GTTT'AAAC Cuts at: 5090 5090 Size: 7786 PmlI CAC'GTG Cuts at: 2278 3820 2278 Size: 1542 6244 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3830 3862 5855 5887 7368 7400 3830 Size: 32 1993 32 1481 32 4216 Fragments arranged by size: 4216 1993 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2143 3657 4276 2143 Size: 1514 619 5653 Fragments arranged by size: 5653 1514 619 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7786 PsiI TTA'TAA Cuts at: 5356 5356 Size: 7786 PspOMI G'GGCC_C Cuts at: 4297 4297 Size: 7786 PspXI vC'TCGA_Gb Cuts at: 2951 2951 Size: 7786 PstI C_TGCA'G Cuts at: 830 2274 2417 3044 3478 3894 5138 830 Size: 1444 143 627 434 416 1244 3478 Fragments arranged by size: 3478 1444 1244 627 434 416 143 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7786 PvuII CAG'CTG Cuts at: 3283 3613 3844 3895 3283 Size: 330 231 51 7174 Fragments arranged by size: 7174 330 231 51 RsrII CG'GwC_CG Cuts at: 3336 4447 3336 Size: 1111 6675 SacI G_AGCT'C Cuts at: 2952 2952 Size: 7786 SacII CC_GC'GG Cuts at: 5053 5053 Size: 7786 SalI G'TCGA_C Cuts at: 1936 5128 1936 Size: 3192 4594 SanDI GG'GwC_CC Cuts at: 3657 3657 Size: 7786 SapI GCTCTTCn'nnn_ Cuts at: 2441 3831 2441 Size: 1390 6396 SbfI CC_TGCA'GG Cuts at: 3478 5138 3478 Size: 1660 6126 ScaI AGT'ACT Cuts at: 2390 4817 6034 7746 2390 Size: 2427 1217 1712 2430 Fragments arranged by size: 2430 2427 1712 1217 SexAI A'CCwGG_T Cuts at: 3296 3963 3296 Size: 667 7119 SfcI C'TryA_G Cuts at: 826 1045 2147 2270 2413 3040 3474 3890 5134 Size: 219 1102 123 143 627 434 416 1244 Cuts at: 5134 6269 6950 7141 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1244 1135 1102 681 627 434 416 219 191 143 123 SfiI GGCCn_nnn'nGGCC Cuts at: 4604 4604 Size: 7786 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7786 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7786 SmaI CCC'GGG Cuts at: 1872 3349 5113 1872 Size: 1477 1764 4545 Fragments arranged by size: 4545 1764 1477 SmlI C'TyrA_G Cuts at: 820 1017 1943 2951 3680 5005 5890 6791 7053 Size: 197 926 1008 729 1325 885 901 262 Cuts at: 7053 820 Size: 1553 Fragments arranged by size: 1553 1325 1008 926 901 885 729 262 197 SnaBI TAC'GTA Cuts at: 493 5083 5577 493 Size: 4590 494 2702 Fragments arranged by size: 4590 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7786 SphI G_CATG'C Cuts at: 1132 4354 5144 1132 Size: 3222 790 3774 Fragments arranged by size: 3774 3222 790 SspI AAT'ATT Cuts at: 5 52 5710 5 Size: 47 5658 2081 Fragments arranged by size: 5658 2081 47 StuI AGG'CCT Cuts at: 1517 2801 1517 Size: 1284 6502 StyI C'CwwG_G Cuts at: 513 1065 2022 2337 2445 3100 3228 4179 5225 Size: 552 957 315 108 655 128 951 1046 Cuts at: 5225 513 Size: 3074 Fragments arranged by size: 3074 1046 957 951 655 552 315 128 108 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2206 2566 2834 4914 5813 5830 5983 Size: 1255 626 360 268 2080 899 17 153 Cuts at: 5983 6168 6613 325 Size: 185 445 1498 Fragments arranged by size: 2080 1498 1255 899 626 445 360 268 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2388 4815 6032 Size: 274 80 33 51 1443 411 2427 1217 Cuts at: 6032 7744 96 Size: 1712 138 Fragments arranged by size: 2427 1712 1443 1217 411 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7746 7778 7746 Size: 32 7754 Tth111I GACn'n_nGTC Cuts at: 1316 2141 2753 2860 1316 Size: 825 612 107 6242 Fragments arranged by size: 6242 825 612 107 XbaI T'CTAG_A Cuts at: 5122 5122 Size: 7786 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3235 4186 4942 3235 Size: 951 756 6079 Fragments arranged by size: 6079 951 756 XhoI C'TCGA_G Cuts at: 1943 2951 1943 Size: 1008 6778 XmaI C'CCGG_G Cuts at: 1870 3347 5111 1870 Size: 1477 1764 4545 Fragments arranged by size: 4545 1764 1477 XmnI GAAnn'nnTTC Cuts at: 2834 4991 5915 2834 Size: 2157 924 4705 Fragments arranged by size: 4705 2157 924 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4459 5594 276 Size: 53 83 186 936 2925 1135 2468 Fragments arranged by size: 2925 2468 1135 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BbeI BfrBI BsiWI BstZ17I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PsrI SfoI SrfI SwaI