Restriction map for
pFN21ASDA0842
(Linear) (Six-base) MAPSORT of: KIAA0842.seq Check: 8612 from: 1 to: 3057
KIAA0842.orf
With 182 enzymes: SgfI *
February 28, 2007 11:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1784 3057
Size: 1784 1273
AatII G_ACGT'C
Cuts at: 0 2438 3057
Size: 2438 619
AccI GT'mk_AC
Cuts at: 0 1763 1904 3057
Size: 1763 141 1153
Fragments arranged by size:
1763 1153 141
AfeI AGC'GCT
Cuts at: 0 1162 1213 1692 3057
Size: 1162 51 479 1365
Fragments arranged by size:
1365 1162 479 51
AflIII A'CryG_T
Cuts at: 0 1796 3057
Size: 1796 1261
AhdI GACnn_n'nnGTC
Cuts at: 0 573 1980 3057
Size: 573 1407 1077
Fragments arranged by size:
1407 1077 573
AleI CACnn'nnGTG
Cuts at: 0 859 3057
Size: 859 2198
AlwNI CAG_nnn'CTG
Cuts at: 0 132 522 835 900 1227 1274 1578 1630
Size: 132 390 313 65 327 47 304 52
Cuts at: 1630 1875 2043 2654 3002 3023 3057
Size: 245 168 611 348 21 34
Fragments arranged by size:
611 390 348 327 313 304 245 168
132 65 52 47 34 21
ApaI G_GGCC'C
Cuts at: 0 2278 3057
Size: 2278 779
ApaLI G'TGCA_C
Cuts at: 0 862 2019 2242 3057
Size: 862 1157 223 815
Fragments arranged by size:
1157 862 815 223
ApoI r'AATT_y
Cuts at: 0 2179 2968 3057
Size: 2179 789 89
Fragments arranged by size:
2179 789 89
AvaI C'yCGr_G
Cuts at: 0 928 1324 2302 3041 3057
Size: 928 396 978 739 16
Fragments arranged by size:
978 928 739 396 16
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 502 535 3057
Size: 502 33 2522
Fragments arranged by size:
2522 502 33
BamHI G'GATC_C
Cuts at: 0 26 2405 3057
Size: 26 2379 652
Fragments arranged by size:
2379 652 26
BanI G'GyrC_C
Cuts at: 0 2308 2347 3057
Size: 2308 39 710
Fragments arranged by size:
2308 710 39
BanII G_rGCy'C
Cuts at: 0 793 929 1295 1729 2278 3057
Size: 793 136 366 434 549 779
Fragments arranged by size:
793 779 549 434 366 136
BbsI GAAGACnn'nnnn_
Cuts at: 0 542 2383 3057
Size: 542 1841 674
Fragments arranged by size:
1841 674 542
BbvCI CC'TCA_GC
Cuts at: 0 1694 3057
Size: 1694 1363
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 196 230 909 943 1221 1255 3057
Size: 196 34 679 34 278 34 1802
Fragments arranged by size:
1802 679 278 196 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1548 3057
Size: 1548 1509
BclI T'GATC_A
Cuts at: 0 2105 3057
Size: 2105 952
BglI GCCn_nnn'nGGC
Cuts at: 0 1064 2581 3057
Size: 1064 1517 476
Fragments arranged by size:
1517 1064 476
BlpI GC'TnA_GC
Cuts at: 0 1289 2395 3057
Size: 1289 1106 662
Fragments arranged by size:
1289 1106 662
Bme1580I G_kGCm'C
Cuts at: 0 866 1853 2023 2246 2278 2311 2350 2743
Size: 866 987 170 223 32 33 39 393
Cuts at: 2743 3057
Size: 314
Fragments arranged by size:
987 866 393 314 223 170 39 33
32
BmgBI CAC'GTC
Cuts at: 0 2381 3057
Size: 2381 676
BmrI ACTGGGnnnn_n'
Cuts at: 0 201 714 2256 3057
Size: 201 513 1542 801
Fragments arranged by size:
1542 801 513 201
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 57 1586 3057
Size: 57 1529 1471
Fragments arranged by size:
1529 1471 57
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 634 1600 2572 3057
Size: 52 582 966 972 485
Fragments arranged by size:
972 966 582 485 52
Bpu10I CC'TnA_GC
Cuts at: 0 1228 1571 1694 2854 3057
Size: 1228 343 123 1160 203
Fragments arranged by size:
1228 1160 343 203 123
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1642 3003 3057
Size: 1642 1361 54
Fragments arranged by size:
1642 1361 54
BsaI GGTCTCn'nnnn_
Cuts at: 0 710 1658 3038 3057
Size: 710 948 1380 19
Fragments arranged by size:
1380 948 710 19
BsaAI yAC'GTr
Cuts at: 0 255 1797 1881 2699 3057
Size: 255 1542 84 818 358
Fragments arranged by size:
1542 818 358 255 84
BsaHI Gr'CG_yC
Cuts at: 0 1537 2435 3057
Size: 1537 898 622
Fragments arranged by size:
1537 898 622
BsaWI w'CCGG_w
Cuts at: 0 23 104 1835 1913 3057
Size: 23 81 1731 78 1144
Fragments arranged by size:
1731 1144 81 78 23
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 295 325 1305 1335 3003 3033 3057
Size: 295 30 980 30 1668 30 24
Fragments arranged by size:
1668 980 295 30 30 30 24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 171 834 835 901 1006 1135 1532 1535
Size: 171 663 1 66 105 129 397 3
Cuts at: 1535 1538 2821 3057
Size: 3 1283 236
Fragments arranged by size:
1283 663 397 236 171 129 105 66
3 3 1
BseYI C'CCAG_C
Cuts at: 0 547 694 797 1267 1586 1672 2134 2647
Size: 547 147 103 470 319 86 462 513
Cuts at: 2647 2751 3057
Size: 104 306
Fragments arranged by size:
547 513 470 462 319 306 147 104
103 86
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 903 2317 2844 2873 3057
Size: 903 1414 527 29 184
Fragments arranged by size:
1414 903 527 184 29
BsiEI CG_ry'CG
Cuts at: 0 2541 3033 3057
Size: 2541 492 24
Fragments arranged by size:
2541 492 24
BsiHKAI G_wGCw'C
Cuts at: 0 143 200 866 929 1282 1846 2023 2246
Size: 143 57 666 63 353 564 177 223
Cuts at: 2246 2396 3057
Size: 150 661
Fragments arranged by size:
666 661 564 353 223 177 150 143
63 57
BsmI GAATG_Cn'
Cuts at: 0 384 3057
Size: 384 2673
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2775 3057
Size: 2775 282
Bsp1286I G_dGCh'C
Cuts at: 0 143 200 793 866 929 1282 1295 1729
Size: 143 57 593 73 63 353 13 434
Cuts at: 1729 1846 1853 2023 2246 2278 2311 2350 2396
Size: 117 7 170 223 32 33 39 46
Cuts at: 2396 2743 3057
Size: 347 314
Fragments arranged by size:
593 434 353 347 314 223 170 143
117 73 63 57 46 39 33 32
13 7
BspEI T'CCGG_A
Cuts at: 0 104 1835 1913 3057
Size: 104 1731 78 1144
Fragments arranged by size:
1731 1144 104 78
BspHI T'CATG_A
Cuts at: 0 1787 3057
Size: 1787 1270
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 335 530 1784 1874 1916 3057
Size: 335 195 1254 90 42 1141
Fragments arranged by size:
1254 1141 335 195 90 42
BsrBI CCG'CTC
Cuts at: 0 746 2732 3057
Size: 746 1986 325
Fragments arranged by size:
1986 746 325
BsrFI r'CCGG_y
Cuts at: 0 825 1315 2540 3057
Size: 825 490 1225 517
Fragments arranged by size:
1225 825 517 490
BssSI C'ACGA_G
Cuts at: 0 199 1560 3057
Size: 199 1361 1497
Fragments arranged by size:
1497 1361 199
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1277 2043 2663 3057
Size: 1277 766 620 394
Fragments arranged by size:
1277 766 620 394
BstEII G'GTnAC_C
Cuts at: 0 942 3057
Size: 942 2115
BstXI CCAn_nnnn'nTGG
Cuts at: 0 267 612 2184 3057
Size: 267 345 1572 873
Fragments arranged by size:
1572 873 345 267
BstYI r'GATC_y
Cuts at: 0 26 92 459 2405 3057
Size: 26 66 367 1946 652
Fragments arranged by size:
1946 652 367 66 26
Bsu36I CC'TnA_GG
Cuts at: 0 1490 3057
Size: 1490 1567
BtgI C'CryG_G
Cuts at: 0 558 1145 1205 2156 2501 3027 3057
Size: 558 587 60 951 345 526 30
Fragments arranged by size:
951 587 558 526 345 60 30
BtsI GCAGTG_nn'
Cuts at: 0 686 2289 2479 3057
Size: 686 1603 190 578
Fragments arranged by size:
1603 686 578 190
DraIII CAC_nnn'GTG
Cuts at: 0 1277 3057
Size: 1277 1780
DrdI GACnn_nn'nnGTC
Cuts at: 0 426 1900 3057
Size: 426 1474 1157
Fragments arranged by size:
1474 1157 426
EaeI y'GGCC_r
Cuts at: 0 257 1437 1461 1500 2174 2582 3030 3057
Size: 257 1180 24 39 674 408 448 27
Fragments arranged by size:
1180 674 448 408 257 39 27 24
EagI C'GGCC_G
Cuts at: 0 3030 3057
Size: 3030 27
EarI CTCTTCn'nnn_
Cuts at: 0 418 1505 1808 1943 3057
Size: 418 1087 303 135 1114
Fragments arranged by size:
1114 1087 418 303 135
EcoICRI GAG'CTC
Cuts at: 0 927 3057
Size: 927 2130
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 634 1600 2572 3057
Size: 52 582 966 972 485
Fragments arranged by size:
972 966 582 485 52
EcoNI CCTnn'n_nnAGG
Cuts at: 0 783 1231 2362 3057
Size: 783 448 1131 695
Fragments arranged by size:
1131 783 695 448
EcoO109I rG'GnC_Cy
Cuts at: 0 120 897 1609 1634 2253 2275 3057
Size: 120 777 712 25 619 22 782
Fragments arranged by size:
782 777 712 619 120 25 22
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 559 2400 3057
Size: 559 1841 657
Fragments arranged by size:
1841 657 559
HaeII r_GCGC'y
Cuts at: 0 804 1164 1215 1694 2068 2768 3002 3057
Size: 804 360 51 479 374 700 234 55
Fragments arranged by size:
804 700 479 374 360 234 55 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 166 198 212 244 559 591 823 855
Size: 166 32 14 32 315 32 232 32
Cuts at: 855 1305 1337 1966 1998 2583 2615 3003 3035
Size: 450 32 629 32 585 32 388 32
Cuts at: 3035 3057
Size: 22
Fragments arranged by size:
629 585 450 388 315 232 166 32
32 32 32 32 32 32 32 22
14
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 782 2561 2981 3057
Size: 782 1779 420 76
Fragments arranged by size:
1779 782 420 76
MscI TGG'CCA
Cuts at: 0 259 1463 1502 2176 3057
Size: 259 1204 39 674 881
Fragments arranged by size:
1204 881 674 259 39
MslI CAynn'nnrTG
Cuts at: 0 859 1204 3057
Size: 859 345 1853
Fragments arranged by size:
1853 859 345
MspA1I CmG'CkG
Cuts at: 0 1260 1470 1590 1821 1872 3009 3029 3057
Size: 1260 210 120 231 51 1137 20 28
Fragments arranged by size:
1260 1137 231 210 120 51 28 20
NaeI GCC'GGC
Cuts at: 0 827 3057
Size: 827 2230
NcoI C'CATG_G
Cuts at: 0 1205 2156 3057
Size: 1205 951 901
Fragments arranged by size:
1205 951 901
NgoMIV G'CCGG_C
Cuts at: 0 825 3057
Size: 825 2232
NspI r_CATG'y
Cuts at: 0 83 498 1477 2331 3057
Size: 83 415 979 854 726
Fragments arranged by size:
979 854 726 415 83
PasI CC'CwG_GG
Cuts at: 0 2271 2634 2840 3057
Size: 2271 363 206 217
Fragments arranged by size:
2271 363 217 206
PflMI CCAn_nnn'nTGG
Cuts at: 0 900 1078 1274 1630 3057
Size: 900 178 196 356 1427
Fragments arranged by size:
1427 900 356 196 178
PfoI T'CCnGG_A
Cuts at: 0 29 338 2945 3057
Size: 29 309 2607 112
Fragments arranged by size:
2607 309 112 29
PmlI CAC'GTG
Cuts at: 0 255 1797 3057
Size: 255 1542 1260
Fragments arranged by size:
1542 1260 255
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1807 1839 3057
Size: 1807 32 1218
Fragments arranged by size:
1807 1218 32
PpuMI rG'GwC_Cy
Cuts at: 0 120 1634 2253 3057
Size: 120 1514 619 804
Fragments arranged by size:
1514 804 619 120
PspOMI G'GGCC_C
Cuts at: 0 2274 3057
Size: 2274 783
PspXI vC'TCGA_Gb
Cuts at: 0 928 3057
Size: 928 2129
PstI C_TGCA'G
Cuts at: 0 251 394 1021 1455 1871 3057
Size: 251 143 627 434 416 1186
Fragments arranged by size:
1186 627 434 416 251 143
PvuII CAG'CTG
Cuts at: 0 1260 1590 1821 1872 3057
Size: 1260 330 231 51 1185
Fragments arranged by size:
1260 1185 330 231 51
RsrII CG'GwC_CG
Cuts at: 0 1313 2424 3057
Size: 1313 1111 633
Fragments arranged by size:
1313 1111 633
SacI G_AGCT'C
Cuts at: 0 929 3057
Size: 929 2128
SacII CC_GC'GG
Cuts at: 0 3030 3057
Size: 3030 27
SanDI GG'GwC_CC
Cuts at: 0 1634 3057
Size: 1634 1423
SapI GCTCTTCn'nnn_
Cuts at: 0 418 1808 3057
Size: 418 1390 1249
Fragments arranged by size:
1390 1249 418
SbfI CC_TGCA'GG
Cuts at: 0 1455 3057
Size: 1455 1602
ScaI AGT'ACT
Cuts at: 0 367 2794 3057
Size: 367 2427 263
Fragments arranged by size:
2427 367 263
SexAI A'CCwGG_T
Cuts at: 0 1273 1940 3057
Size: 1273 667 1117
Fragments arranged by size:
1273 1117 667
SfcI C'TryA_G
Cuts at: 0 124 247 390 1017 1451 1867 3057
Size: 124 123 143 627 434 416 1190
Fragments arranged by size:
1190 627 434 416 143 124 123
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2581 3057
Size: 2581 476
SmaI CCC'GGG
Cuts at: 0 1326 3057
Size: 1326 1731
SmlI C'TyrA_G
Cuts at: 0 928 1657 2982 3057
Size: 928 729 1325 75
Fragments arranged by size:
1325 928 729 75
SphI G_CATG'C
Cuts at: 0 2331 3057
Size: 2331 726
StuI AGG'CCT
Cuts at: 0 778 3057
Size: 778 2279
StyI C'CwwG_G
Cuts at: 0 314 422 1077 1205 2156 3057
Size: 314 108 655 128 951 901
Fragments arranged by size:
951 901 655 314 128 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 183 543 811 2891 3057
Size: 183 360 268 2080 166
Fragments arranged by size:
2080 360 268 183 166
TatI w'GTAC_w
Cuts at: 0 365 2792 3057
Size: 365 2427 265
Fragments arranged by size:
2427 365 265
Tth111I GACn'n_nGTC
Cuts at: 0 118 730 837 3057
Size: 118 612 107 2220
Fragments arranged by size:
2220 612 118 107
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1212 2163 2919 3057
Size: 1212 951 756 138
Fragments arranged by size:
1212 951 756 138
XhoI C'TCGA_G
Cuts at: 0 928 3057
Size: 928 2129
XmaI C'CCGG_G
Cuts at: 0 1324 3057
Size: 1324 1733
XmnI GAAnn'nnTTC
Cuts at: 0 811 2968 3057
Size: 811 2157 89
Fragments arranged by size:
2157 811 89
ZraI GAC'GTC
Cuts at: 0 2436 3057
Size: 2436 621
Enzymes that do cut:
AarI AatII AccI AfeI AflIII AhdI AleI AlwNI
ApaI ApaLI ApoI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI
BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI
DraIII DrdI EaeI EagI EarI EcoICRI Eco57MI EcoNI
EcoO109I FalI HaeII Hin4I MmeI MscI MslI MspA1I
NaeI NcoI NgoMIV NspI PasI PflMI PfoI PmlI
PpiI PpuMI PspOMI PspXI PstI PvuII RsrII SacI
SacII SanDI SapI SbfI ScaI SexAI SfcI SfiI
SmaI SmlI SphI StuI StyI TaqII TatI Tth111I
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AcuI AflII AgeI AlfI AloI AscI
AseI AsiSI AvrII BbeI BfrBI BglII BmtI BsaBI
BsiWI BsrDI BsrGI BssHII BstBI BstZ17I BtgZI ClaI
CspCI DraI EciI EcoRI EcoRV FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MfeI MluI NarI
NdeI NheI NotI NruI NsiI PacI PciI PmeI
PshAI PsiI PsrI PvuI SalI SfoI SgfI SgrAI
SnaBI SpeI SrfI SspI SwaI TstI XbaI