(Circular) (Six-base) MAPSORT of: pFN21ASDA0814.seq Check: 9738 from: 1 to: 9298 pFN21ASDA0814 9298 bp With 182 enzymes: SgfI * January 31, 2011 17:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4904 4937 5060 5327 5456 5566 4904 Size: 33 123 267 129 110 8636 Fragments arranged by size: 8636 267 129 123 110 33 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7108 278 Size: 53 83 186 936 5572 2468 Fragments arranged by size: 5572 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6641 1937 Size: 4704 4594 Acc65I G'GTAC_C Cuts at: 6619 6619 Size: 9298 AclI AA'CG_TT Cuts at: 1563 7425 7798 1563 Size: 5862 373 3063 Fragments arranged by size: 5862 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2366 3041 4390 4560 5317 7361 8745 9013 Size: 734 675 1349 170 757 2044 1384 268 Cuts at: 9013 1632 Size: 1917 Fragments arranged by size: 2044 1917 1384 1349 757 734 675 268 170 AfeI AGC'GCT Cuts at: 2559 2559 Size: 9298 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9101 AflIII A'CryG_T Cuts at: 1203 2805 3213 4413 5076 6189 8197 9174 1203 Size: 1602 408 1200 663 1113 2008 977 1327 Fragments arranged by size: 2008 1602 1327 1200 1113 977 663 408 AgeI A'CCGG_T Cuts at: 8129 8129 Size: 9298 AhdI GACnn_n'nnGTC Cuts at: 6380 8027 6380 Size: 1647 7651 AleI CACnn'nnGTG Cuts at: 1166 2495 3566 4938 5054 1166 Size: 1329 1071 1372 116 5410 Fragments arranged by size: 5410 1372 1329 1071 116 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4403 5447 1184 Size: 3219 1044 5035 Fragments arranged by size: 5035 3219 1044 AlwNI CAG_nnn'CTG Cuts at: 2344 3082 3226 3589 3778 5770 5998 7041 8613 Size: 738 144 363 189 1992 228 1043 1572 Cuts at: 8613 2344 Size: 3029 Fragments arranged by size: 3029 1992 1572 1043 738 363 228 189 144 ApaI G_GGCC'C Cuts at: 3409 5284 5419 6474 3409 Size: 1875 135 1055 6233 Fragments arranged by size: 6233 1875 1055 135 ApaLI G'TGCA_C Cuts at: 3809 4207 6523 7355 8511 3809 Size: 398 2316 832 1156 4596 Fragments arranged by size: 4596 2316 1156 832 398 ApoI r'AATT_y Cuts at: 1493 6607 6833 6985 9094 1493 Size: 5114 226 152 2109 1697 Fragments arranged by size: 5114 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 7852 160 Size: 872 6820 1606 Fragments arranged by size: 6820 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9298 AvaI C'yCGr_G Cuts at: 1870 1943 2030 2190 2467 3539 6126 6623 1870 Size: 73 87 160 277 1072 2587 497 4545 Fragments arranged by size: 4545 2587 1072 497 277 160 87 73 AvrII C'CTAG_G Cuts at: 4085 4085 Size: 9298 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 5065 5098 5065 Size: 33 9265 BamHI G'GATC_C Cuts at: 2463 6628 6995 2463 Size: 4165 367 4766 Fragments arranged by size: 4766 4165 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2048 2330 4089 4925 6439 Size: 325 217 627 261 282 1759 836 1514 Cuts at: 6439 6619 8074 618 Size: 180 1455 1842 Fragments arranged by size: 1842 1759 1514 1455 836 627 325 282 261 217 180 BanII G_rGCy'C Cuts at: 1391 2274 2832 3288 3409 4190 4251 4689 5284 Size: 883 558 456 121 781 61 438 595 Cuts at: 5284 5419 5445 6312 6474 6617 1391 Size: 135 26 867 162 143 4072 Fragments arranged by size: 4072 883 867 781 595 558 456 438 162 143 135 121 61 26 BbeI G_GCGC'C Cuts at: 2052 2334 2052 Size: 282 9016 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2307 4607 6508 928 Size: 971 408 2300 1901 3718 Fragments arranged by size: 3718 2300 1901 971 408 BbvCI CC'TCA_GC Cuts at: 3857 4454 3857 Size: 597 8701 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2872 2906 5027 5061 7489 7523 1903 Size: 34 935 34 2121 34 2428 34 3678 Fragments arranged by size: 3678 2428 2121 935 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7190 8400 7190 Size: 1210 8088 BclI T'GATC_A Cuts at: 1548 1548 Size: 9298 BfrBI ATG'CAT Cuts at: 5937 6416 5937 Size: 479 8819 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2069 3837 4980 Size: 107 122 71 631 605 397 1768 1143 Cuts at: 4980 5328 5463 6093 6330 7909 136 Size: 348 135 630 237 1579 1525 Fragments arranged by size: 1768 1579 1525 1143 631 630 605 397 348 237 135 122 107 71 BglII A'GATC_T Cuts at: 9293 9293 Size: 9298 BlpI GC'TnA_GC Cuts at: 2211 4184 6259 6715 2211 Size: 1973 2075 456 4794 Fragments arranged by size: 4794 2075 1973 456 Bme1580I G_kGCm'C Cuts at: 1790 2813 3409 3539 3563 3813 4211 4326 4887 Size: 1023 596 130 24 250 398 115 561 Cuts at: 4887 5284 5419 5906 6444 6474 6527 7359 8515 Size: 397 135 487 538 30 53 832 1156 Cuts at: 8515 1790 Size: 2573 Fragments arranged by size: 2573 1156 1023 832 596 561 538 487 398 397 250 135 130 115 53 30 24 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9298 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3001 5278 5413 Size: 416 53 83 399 24 1584 2277 135 Cuts at: 5413 6186 6469 7029 7987 442 Size: 773 283 560 958 1753 Fragments arranged by size: 2277 1753 1584 958 773 560 416 399 283 135 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9298 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2124 5706 2124 Size: 3582 5716 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2094 3048 3362 3683 3821 4771 5660 5801 Size: 855 954 314 321 138 950 889 141 Cuts at: 5801 7958 1239 Size: 2157 2579 Fragments arranged by size: 2579 2157 954 950 889 855 321 314 141 138 Bpu10I CC'TnA_GC Cuts at: 3478 3857 4367 4439 4454 4683 5142 3478 Size: 379 510 72 15 229 459 7634 Fragments arranged by size: 7634 510 459 379 229 72 15 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2258 4094 7423 8288 8586 2258 Size: 1836 3329 865 298 2970 Fragments arranged by size: 3329 2970 1836 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3797 7961 882 Size: 617 2298 4164 2219 Fragments arranged by size: 4164 2298 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2806 2877 5077 5527 5659 6595 7089 Size: 711 1602 71 2200 450 132 936 494 Cuts at: 7089 493 Size: 2702 Fragments arranged by size: 2702 2200 1602 936 711 494 450 132 71 BsaBI GATnn'nnATC Cuts at: 4121 9292 4121 Size: 5171 4127 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2049 2331 7105 7487 Size: 53 83 186 936 516 282 4774 382 Cuts at: 7487 8122 275 Size: 635 1451 Fragments arranged by size: 4774 1451 936 635 516 382 282 186 83 53 BsaWI w'CCGG_w Cuts at: 7731 8129 8403 8550 8961 7731 Size: 398 274 147 411 8068 Fragments arranged by size: 8068 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2792 2822 2840 2870 3156 Size: 30 165 30 1389 30 18 30 286 Cuts at: 3156 3186 3230 3260 1178 Size: 30 44 30 7216 Fragments arranged by size: 7216 1389 286 165 44 30 30 30 30 30 30 18 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3164 3167 3874 4181 6526 1186 Size: 1978 3 707 307 2345 3958 Fragments arranged by size: 3958 2345 1978 707 307 3 BseYI C'CCAG_C Cuts at: 2170 2267 2812 4582 8501 8997 2170 Size: 97 545 1770 3919 496 2471 Fragments arranged by size: 3919 2471 1770 545 496 97 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2810 2857 2899 3791 4861 5453 6010 Size: 573 1074 47 42 892 1070 592 557 Cuts at: 6010 1163 Size: 4451 Fragments arranged by size: 4451 1074 1070 892 592 573 557 47 42 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6748 7509 8537 1540 Size: 275 186 4747 761 1028 2301 Fragments arranged by size: 4747 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2417 3813 4211 6348 6527 7359 7444 8515 2417 Size: 1396 398 2137 179 832 85 1071 3200 Fragments arranged by size: 3200 2137 1396 1071 832 398 179 85 BsmI GAATG_Cn' Cuts at: 6809 6902 6809 Size: 93 9205 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2274 2417 2813 2832 3288 3409 3539 Size: 399 484 143 396 19 456 121 130 Cuts at: 3539 3563 3813 4190 4211 4251 4326 4689 4887 Size: 24 250 377 21 40 75 363 198 Cuts at: 4887 5284 5419 5445 5906 6312 6348 6444 6474 Size: 397 135 26 461 406 36 96 30 Cuts at: 6474 6527 6617 7359 7444 8515 1391 Size: 53 90 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 484 461 456 406 399 397 396 377 363 250 198 143 135 130 121 96 90 85 75 53 40 36 30 26 24 21 19 BspEI T'CCGG_A Cuts at: 8961 8961 Size: 9298 BspHI T'CATG_A Cuts at: 7187 8917 7187 Size: 1730 7568 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2147 2514 3234 3492 3639 3705 4904 4937 Size: 1303 367 720 258 147 66 1199 33 Cuts at: 4937 5060 5327 5456 5566 6653 844 Size: 123 267 129 110 1087 3489 Fragments arranged by size: 3489 1303 1199 1087 720 367 267 258 147 129 123 110 66 33 BsrBI CCG'CTC Cuts at: 1394 1823 6054 7185 1394 Size: 429 4231 1131 3507 Fragments arranged by size: 4231 3507 1131 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2848 4525 4780 7793 7967 65 Size: 1183 1600 1677 255 3013 174 1396 Fragments arranged by size: 3013 1677 1600 1396 1183 255 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3899 4449 5471 5879 5910 6101 7942 Size: 110 2089 550 1022 408 31 191 1841 Cuts at: 7942 8129 1700 Size: 187 2869 Fragments arranged by size: 2869 2089 1841 1022 550 408 191 187 110 31 BsrGI T'GTAC_A Cuts at: 96 5703 96 Size: 5607 3691 BssHII G'CGCG_C Cuts at: 1925 2059 1925 Size: 134 9164 BssSI C'ACGA_G Cuts at: 3530 7358 8370 3530 Size: 3828 1012 4458 Fragments arranged by size: 4458 3828 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3516 4779 4875 6024 6033 6422 794 Size: 2722 1263 96 1149 9 389 3670 Fragments arranged by size: 3670 2722 1263 1149 389 96 9 BstBI TT'CG_AA Cuts at: 2007 8102 2007 Size: 6095 3203 BstEII G'GTnAC_C Cuts at: 1184 2614 1184 Size: 1430 7868 BstXI CCAn_nnnn'nTGG Cuts at: 4955 5056 4955 Size: 101 9197 BstYI r'GATC_y Cuts at: 1972 2454 2463 2574 2640 6628 6995 7380 7397 Size: 482 9 111 66 3988 367 385 17 Cuts at: 7397 8849 8935 8947 9293 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 3988 1977 1452 482 385 367 346 111 86 66 17 12 9 BtgI C'CryG_G Cuts at: 513 1065 2022 2164 2482 3826 4513 4689 5275 Size: 552 957 142 318 1344 687 176 586 Cuts at: 5275 5364 5929 6057 6157 6277 513 Size: 89 565 128 100 120 3534 Fragments arranged by size: 3534 1344 957 687 586 565 552 318 176 142 128 120 100 89 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3081 4246 4648 487 Size: 683 56 1855 1165 402 5137 Fragments arranged by size: 5137 1855 1165 683 402 56 BtsI GCAGTG_nn' Cuts at: 4211 5838 6290 6346 6814 7608 7628 4211 Size: 1627 452 56 468 794 20 5881 Fragments arranged by size: 5881 1627 794 468 452 56 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9298 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9263 DraI TTT'AAA Cuts at: 6602 6602 Size: 9298 DraIII CAC_nnn'GTG Cuts at: 2133 5137 5743 6033 2133 Size: 3004 606 290 5398 Fragments arranged by size: 5398 3004 606 290 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2309 8305 809 Size: 507 993 5996 1802 Fragments arranged by size: 5996 1802 993 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2731 3366 4134 Size: 54 1425 50 275 18 901 635 768 Cuts at: 4134 4764 5580 6142 6745 7634 9263 8 Size: 630 816 562 603 889 1629 43 Fragments arranged by size: 1629 1425 901 889 816 768 635 630 603 562 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6745 1537 Size: 275 4933 4090 Fragments arranged by size: 4933 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3293 4055 6543 7228 1353 Size: 1940 762 2488 685 3423 Fragments arranged by size: 3423 2488 1940 762 685 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2798 4081 4327 5079 5530 6386 Size: 1064 138 1209 1283 246 752 451 856 Cuts at: 6386 6520 7885 8259 8405 387 Size: 134 1365 374 146 1280 Fragments arranged by size: 1365 1283 1280 1209 1064 856 752 451 374 246 146 138 134 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2094 2366 3041 3048 3362 3683 3821 Size: 393 462 272 675 7 314 321 138 Cuts at: 3821 4390 4560 4771 5317 5660 5801 7361 7958 Size: 569 170 211 546 343 141 1560 597 Cuts at: 7958 8745 9013 1239 Size: 787 268 1524 Fragments arranged by size: 1560 1524 787 675 597 569 546 462 393 343 321 314 272 268 211 170 141 138 7 EcoO109I rG'GnC_Cy Cuts at: 2341 3079 3223 3405 3963 4822 5280 5415 5980 Size: 738 144 182 558 859 458 135 565 Cuts at: 5980 6130 6180 6471 2341 Size: 150 50 291 5168 Fragments arranged by size: 5168 859 738 565 558 458 291 182 150 144 135 50 EcoRI G'AATT_C Cuts at: 6607 6985 6607 Size: 378 8920 EcoRV GAT'ATC Cuts at: 3051 3051 Size: 9298 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2278 3304 2278 Size: 1026 8272 FspI TGC'GCA Cuts at: 5951 7804 5951 Size: 1853 7445 FspAI rTGC'GCAy Cuts at: 5951 5951 Size: 9298 HaeII r_GCGC'y Cuts at: 1678 2052 2075 2087 2218 2334 2561 4583 8445 Size: 374 23 12 131 116 227 2022 3862 Cuts at: 8445 1678 Size: 2531 Fragments arranged by size: 3862 2531 2022 374 227 131 116 23 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2632 2664 2819 2851 4547 Size: 32 135 32 1060 32 155 32 1696 Cuts at: 4547 4579 6366 6378 6398 6410 6521 6553 7939 Size: 32 1787 12 20 12 111 32 1386 Cuts at: 7939 7971 8013 8045 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1787 1696 1386 1060 155 135 111 42 32 32 32 32 32 32 32 32 20 12 12 HincII GTy'rAC Cuts at: 669 1657 1938 5503 6642 6888 669 Size: 988 281 3565 1139 246 3079 Fragments arranged by size: 3565 3079 1139 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 9298 HpaI GTT'AAC Cuts at: 6888 6888 Size: 9298 KasI G'GCGC_C Cuts at: 2048 2330 2048 Size: 282 9016 KpnI G_GTAC'C Cuts at: 6623 6623 Size: 9298 MfeI C'AATT_G Cuts at: 6897 6897 Size: 9298 MluI A'CGCG_T Cuts at: 9174 9174 Size: 9298 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2688 3603 4032 4369 4608 5575 5717 8412 Size: 1383 915 429 337 239 967 142 2695 Cuts at: 8412 8596 1305 Size: 184 2007 Fragments arranged by size: 2695 2007 1383 967 915 429 337 239 184 142 MscI TGG'CCA Cuts at: 10 64 1489 1832 2733 4136 4766 5582 10 Size: 54 1425 343 901 1403 630 816 3726 Fragments arranged by size: 3726 1425 1403 901 816 630 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2255 2495 3340 3566 4214 4385 4518 Size: 648 1089 240 845 226 648 171 133 Cuts at: 4518 4938 4953 5054 5429 6190 7256 7615 7774 Size: 420 15 101 375 761 1066 359 159 Cuts at: 7774 518 Size: 2042 Fragments arranged by size: 2042 1089 1066 845 761 648 648 420 375 359 240 226 171 159 133 101 15 MspA1I CmG'CkG Cuts at: 1606 2147 2484 2532 3169 3411 3474 3799 4183 Size: 541 337 48 637 242 63 325 384 Cuts at: 4183 4671 4842 4965 4996 5199 5223 5454 6714 Size: 488 171 123 31 203 24 231 1260 Cuts at: 6714 7391 8539 8784 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1260 1148 677 637 541 488 384 337 325 245 242 231 203 171 123 63 48 31 24 NaeI GCC'GGC Cuts at: 3901 5473 3901 Size: 1572 7726 NarI GG'CG_CC Cuts at: 2049 2331 2049 Size: 282 9016 NcoI C'CATG_G Cuts at: 513 1065 2022 4513 4689 5364 6157 6277 513 Size: 552 957 2491 176 675 793 120 3534 Fragments arranged by size: 3534 2491 957 793 675 552 176 120 NdeI CA'TA_TG Cuts at: 387 387 Size: 9298 NgoMIV G'CCGG_C Cuts at: 3899 5471 3899 Size: 1572 7726 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9298 NotI GC'GGCC_GC Cuts at: 6745 6745 Size: 9298 NsiI A_TGCA'T Cuts at: 5939 6418 5939 Size: 479 8819 NspI r_CATG'y Cuts at: 1132 1229 2635 4273 4390 4417 4815 5316 6193 Size: 97 1406 1638 117 27 398 501 877 Cuts at: 6193 6656 8201 1132 Size: 463 1545 2229 Fragments arranged by size: 2229 1638 1545 1406 877 501 463 398 117 97 27 PasI CC'CwG_GG Cuts at: 1348 3959 5326 5998 1348 Size: 2611 1367 672 4648 Fragments arranged by size: 4648 2611 1367 672 PciI A'CATG_T Cuts at: 4413 6189 8197 4413 Size: 1776 2008 5514 Fragments arranged by size: 5514 2008 1776 PflMI CCAn_nnn'nTGG Cuts at: 1108 2170 3589 4132 5290 1108 Size: 1062 1419 543 1158 5116 Fragments arranged by size: 5116 1419 1158 1062 543 PfoI T'CCnGG_A Cuts at: 2337 3378 4125 4140 2337 Size: 1041 747 15 7495 Fragments arranged by size: 7495 1041 747 15 PmeI GTTT'AAAC Cuts at: 6602 6602 Size: 9298 PmlI CAC'GTG Cuts at: 2877 5659 2877 Size: 2782 6516 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7367 7399 8880 8912 7367 Size: 32 1481 32 7753 Fragments arranged by size: 7753 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2341 3223 4822 6130 6180 2341 Size: 882 1599 1308 50 5459 Fragments arranged by size: 5459 1599 1308 882 50 PshAI GACnn'nnGTC Cuts at: 1613 3922 4611 1613 Size: 2309 689 6300 Fragments arranged by size: 6300 2309 689 PsiI TTA'TAA Cuts at: 6868 6868 Size: 9298 PspOMI G'GGCC_C Cuts at: 3405 5280 5415 6470 3405 Size: 1875 135 1055 6233 Fragments arranged by size: 6233 1875 1055 135 PstI C_TGCA'G Cuts at: 830 2995 3231 3270 3303 3416 4613 4676 5379 Size: 2165 236 39 33 113 1197 63 703 Cuts at: 5379 5577 5970 6650 830 Size: 198 393 680 3478 Fragments arranged by size: 3478 2165 1197 703 680 393 236 198 113 63 39 33 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9298 PvuII CAG'CTG Cuts at: 2532 3169 3799 4183 4965 5199 2532 Size: 637 630 384 782 234 6631 Fragments arranged by size: 6631 782 637 630 384 234 RsrII CG'GwC_CG Cuts at: 5908 5908 Size: 9298 SacII CC_GC'GG Cuts at: 2485 2485 Size: 9298 SalI G'TCGA_C Cuts at: 1936 6640 1936 Size: 4704 4594 SanDI GG'GwC_CC Cuts at: 6130 6130 Size: 9298 SapI GCTCTTCn'nnn_ Cuts at: 3293 3293 Size: 9298 SbfI CC_TGCA'GG Cuts at: 2995 5577 6650 2995 Size: 2582 1073 5643 Fragments arranged by size: 5643 2582 1073 ScaI AGT'ACT Cuts at: 7546 9258 7546 Size: 1712 7586 SexAI A'CCwGG_T Cuts at: 2307 2307 Size: 9298 SfcI C'TryA_G Cuts at: 826 1045 2991 3164 3227 3266 3299 3412 4609 Size: 219 1946 173 63 39 33 113 1197 Cuts at: 4609 4672 4855 5218 5375 5573 5966 6646 7781 Size: 63 183 363 157 198 393 680 1135 Cuts at: 7781 8462 8653 826 Size: 681 191 1471 Fragments arranged by size: 1946 1471 1197 1135 681 680 393 363 219 198 191 183 173 157 113 63 63 39 33 SfiI GGCCn_nnn'nGGCC Cuts at: 3837 3837 Size: 9298 SfoI GGC'GCC Cuts at: 2050 2332 2050 Size: 282 9016 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9298 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9298 SmaI CCC'GGG Cuts at: 1872 2032 6128 6625 1872 Size: 160 4096 497 4545 Fragments arranged by size: 4545 4096 497 160 SmlI C'TyrA_G Cuts at: 820 1017 1943 2273 4109 7402 8303 8565 820 Size: 197 926 330 1836 3293 901 262 1553 Fragments arranged by size: 3293 1836 1553 926 901 330 262 197 SnaBI TAC'GTA Cuts at: 493 6595 7089 493 Size: 6102 494 2702 Fragments arranged by size: 6102 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 9298 SphI G_CATG'C Cuts at: 1132 4273 4390 6656 1132 Size: 3141 117 2266 3774 Fragments arranged by size: 3774 3141 2266 117 SspI AAT'ATT Cuts at: 5 52 7222 5 Size: 47 7170 2081 Fragments arranged by size: 7170 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 9298 StyI C'CwwG_G Cuts at: 513 1065 2022 2079 3135 4085 4275 4513 4689 Size: 552 957 57 1056 950 190 238 176 Cuts at: 4689 5364 5544 5857 6157 6277 6301 6435 6737 Size: 675 180 313 300 120 24 134 302 Cuts at: 6737 513 Size: 3074 Fragments arranged by size: 3074 1056 957 950 675 552 313 302 300 238 190 180 176 134 120 57 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3020 4301 4624 4832 5377 7325 7342 Size: 1255 1440 1281 323 208 545 1948 17 Cuts at: 7342 7495 7680 8125 325 Size: 153 185 445 1498 Fragments arranged by size: 1948 1498 1440 1281 1255 545 445 323 208 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2673 3009 5703 Size: 274 80 33 51 1443 696 336 2694 Cuts at: 5703 7544 9256 96 Size: 1841 1712 138 Fragments arranged by size: 2694 1841 1712 1443 696 336 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2792 2824 3518 3550 4060 4092 5218 5250 5352 Size: 32 694 32 510 32 1126 32 102 Cuts at: 5352 5384 6153 6185 9258 9290 2792 Size: 32 769 32 3073 32 2800 Fragments arranged by size: 3073 2800 1126 769 694 510 102 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9298 XbaI T'CTAG_A Cuts at: 6634 6634 Size: 9298 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3586 4129 5333 3586 Size: 543 1204 7551 Fragments arranged by size: 7551 1204 543 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9298 XmaI C'CCGG_G Cuts at: 1870 2030 6126 6623 1870 Size: 160 4096 497 4545 Fragments arranged by size: 4545 4096 497 160 XmnI GAAnn'nnTTC Cuts at: 2477 7427 2477 Size: 4950 4348 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7106 276 Size: 53 83 186 936 5572 2468 Fragments arranged by size: 5572 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI FseI NruI PacI PspXI PsrI SacI SrfI SwaI