(Circular) (Six-base) MAPSORT of: pFN21ASDA0661.seq Check: 2660 from: 1 to: 7732 pFN21ASDA0661 7732 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3300 4248 3300 Size: 948 6784 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5542 278 Size: 53 83 186 936 4006 2468 Fragments arranged by size: 4006 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4803 5075 1937 Size: 2866 272 4594 Fragments arranged by size: 4594 2866 272 Acc65I G'GTAC_C Cuts at: 5053 5053 Size: 7732 AclI AA'CG_TT Cuts at: 1563 5859 6232 1563 Size: 4296 373 3063 Fragments arranged by size: 4296 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2189 2364 3086 3732 3798 4661 5795 7179 Size: 557 175 722 646 66 863 1134 1384 Cuts at: 7179 7447 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1134 863 722 646 557 268 175 66 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7535 AflIII A'CryG_T Cuts at: 1203 2417 6631 7608 1203 Size: 1214 4214 977 1327 Fragments arranged by size: 4214 1327 1214 977 AgeI A'CCGG_T Cuts at: 6563 6563 Size: 7732 AhdI GACnn_n'nnGTC Cuts at: 6461 6461 Size: 7732 AleI CACnn'nnGTG Cuts at: 1166 3749 1166 Size: 2583 5149 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7732 AlwNI CAG_nnn'CTG Cuts at: 2387 3055 3430 3683 3725 4498 4513 5475 7047 Size: 668 375 253 42 773 15 962 1572 Cuts at: 7047 2387 Size: 3072 Fragments arranged by size: 3072 1572 962 773 668 375 253 42 15 ApaI G_GGCC'C Cuts at: 2073 2320 3456 3583 3811 3818 2073 Size: 247 1136 127 228 7 5987 Fragments arranged by size: 5987 1136 247 228 127 7 ApaLI G'TGCA_C Cuts at: 5789 6945 5789 Size: 1156 6576 ApoI r'AATT_y Cuts at: 1493 5041 5267 5419 7528 1493 Size: 3548 226 152 2109 1697 Fragments arranged by size: 3548 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6286 160 Size: 872 5254 1606 Fragments arranged by size: 5254 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7732 AvaI C'yCGr_G Cuts at: 1870 1943 2712 3258 3825 4547 5057 1870 Size: 73 769 546 567 722 510 4545 Fragments arranged by size: 4545 769 722 567 546 510 73 AvrII C'CTAG_G Cuts at: 3886 3886 Size: 7732 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2165 2198 2165 Size: 33 7699 BamHI G'GATC_C Cuts at: 5062 5429 5062 Size: 367 7365 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2392 2531 3728 4256 4994 Size: 325 217 627 605 139 1197 528 738 Cuts at: 4994 5053 6508 618 Size: 59 1455 1842 Fragments arranged by size: 1842 1455 1197 738 627 605 528 325 217 139 59 BanII G_rGCy'C Cuts at: 1391 2066 2073 2127 2320 2685 2805 2972 3456 Size: 675 7 54 193 365 120 167 484 Cuts at: 3456 3583 3811 3818 3999 4596 4747 5051 1391 Size: 127 228 7 181 597 151 304 4072 Fragments arranged by size: 4072 675 597 484 365 304 228 193 181 167 151 127 120 54 7 7 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2108 2370 3245 3482 928 Size: 971 209 262 875 237 5178 Fragments arranged by size: 5178 971 875 262 237 209 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4165 4199 5923 5957 1903 Size: 34 2228 34 1724 34 3678 Fragments arranged by size: 3678 2228 1724 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5624 6834 5624 Size: 1210 6522 BclI T'GATC_A Cuts at: 1548 1548 Size: 7732 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4628 6343 136 Size: 107 122 71 631 605 2956 1715 1525 Fragments arranged by size: 2956 1715 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7727 7727 Size: 7732 BlpI GC'TnA_GC Cuts at: 5149 5149 Size: 7732 Bme1580I G_kGCm'C Cuts at: 1790 2073 2320 3456 3583 3649 3811 3818 4973 Size: 283 247 1136 127 66 162 7 1155 Cuts at: 4973 4999 5793 6949 1790 Size: 26 794 1156 2573 Fragments arranged by size: 2573 1156 1155 1136 794 283 247 162 127 66 26 7 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7732 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2323 2537 5463 Size: 416 53 83 399 24 906 214 2926 Cuts at: 5463 6421 442 Size: 958 1753 Fragments arranged by size: 2926 1753 958 906 416 399 214 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7732 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4013 4774 4013 Size: 761 6971 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2579 2789 2861 3101 3386 3702 4535 4610 Size: 1340 210 72 240 285 316 833 75 Cuts at: 4610 4673 4811 6392 1239 Size: 63 138 1581 2579 Fragments arranged by size: 2579 1581 1340 833 316 285 240 210 138 75 72 63 Bpu10I CC'TnA_GC Cuts at: 2065 2700 4110 4746 2065 Size: 635 1410 636 5051 Fragments arranged by size: 5051 1410 636 635 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3116 3901 3962 3983 4457 4721 5857 6722 7020 Size: 785 61 21 474 264 1136 865 298 Cuts at: 7020 3116 Size: 3828 Fragments arranged by size: 3828 1136 865 785 474 298 264 61 21 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2457 2822 3989 6395 882 Size: 617 958 365 1167 2406 2219 Fragments arranged by size: 2406 2219 1167 958 617 365 BsaAI yAC'GTr Cuts at: 493 1204 4660 5029 5523 493 Size: 711 3456 369 494 2702 Fragments arranged by size: 3456 2702 711 494 369 BsaBI GATnn'nnATC Cuts at: 7726 7726 Size: 7732 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3522 3639 5539 5921 Size: 53 83 186 936 1989 117 1900 382 Cuts at: 5921 6556 275 Size: 635 1451 Fragments arranged by size: 1989 1900 1451 936 635 382 186 117 83 53 BsaWI w'CCGG_w Cuts at: 6165 6563 6837 6984 7395 6165 Size: 398 274 147 411 6502 Fragments arranged by size: 6502 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7507 Fragments arranged by size: 7507 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2111 2171 2285 2421 2561 2962 3173 3680 Size: 925 60 114 136 140 401 211 507 Cuts at: 3680 3776 3965 4169 4238 4286 4289 4451 4532 Size: 96 189 204 69 48 3 162 81 Cuts at: 4532 4847 1186 Size: 315 4071 Fragments arranged by size: 4071 925 507 401 315 211 204 189 162 140 136 114 96 81 69 60 48 3 BseYI C'CCAG_C Cuts at: 2319 2719 3243 3325 3525 3649 3881 4252 4491 Size: 400 524 82 200 124 232 371 239 Cuts at: 4491 4623 6935 7431 2319 Size: 132 2312 496 2620 Fragments arranged by size: 2620 2312 524 496 400 371 239 232 200 132 124 82 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4103 4526 4667 4682 1163 Size: 573 2367 423 141 15 4213 Fragments arranged by size: 4213 2367 573 423 141 15 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5182 5943 6971 1540 Size: 275 186 3181 761 1028 2301 Fragments arranged by size: 3181 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2805 2972 3309 3999 4596 4659 5793 5878 6949 Size: 167 337 690 597 63 1134 85 1071 Cuts at: 6949 2805 Size: 3588 Fragments arranged by size: 3588 1134 1071 690 597 337 167 85 63 BsmI GAATG_Cn' Cuts at: 3034 4209 5243 5336 3034 Size: 1175 1034 93 5430 Fragments arranged by size: 5430 1175 1034 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3167 3167 Size: 7732 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2066 2073 2127 2320 2685 2805 2972 Size: 399 276 7 54 193 365 120 167 Cuts at: 2972 3309 3456 3583 3649 3811 3818 3999 4596 Size: 337 147 127 66 162 7 181 597 Cuts at: 4596 4659 4747 4973 4999 5051 5793 5878 6949 Size: 63 88 226 26 52 742 85 1071 Cuts at: 6949 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 597 399 365 337 276 226 193 181 167 162 147 127 120 88 85 66 63 54 52 26 7 7 BspEI T'CCGG_A Cuts at: 7395 7395 Size: 7732 BspHI T'CATG_A Cuts at: 5621 7351 5621 Size: 1730 6002 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3300 4248 4450 4871 5087 844 Size: 2456 948 202 421 216 3489 Fragments arranged by size: 3489 2456 948 421 216 202 BsrBI CCG'CTC Cuts at: 1394 1823 2206 3911 4399 5619 1394 Size: 429 383 1705 488 1220 3507 Fragments arranged by size: 3507 1705 1220 488 429 383 BsrDI GCAATG_nn' Cuts at: 65 1248 6227 6401 65 Size: 1183 4979 174 1396 Fragments arranged by size: 4979 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2051 2503 2733 6376 6563 1700 Size: 110 241 452 230 3643 187 2869 Fragments arranged by size: 3643 2869 452 241 230 187 110 BsrGI T'GTAC_A Cuts at: 96 2661 4047 96 Size: 2565 1386 3781 Fragments arranged by size: 3781 2565 1386 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7732 BssSI C'ACGA_G Cuts at: 2601 4713 5792 6804 2601 Size: 2112 1079 1012 3529 Fragments arranged by size: 3529 2112 1079 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2531 794 Size: 1737 5995 BstBI TT'CG_AA Cuts at: 2007 6536 2007 Size: 4529 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7732 BstXI CCAn_nnnn'nTGG Cuts at: 2839 3590 2839 Size: 751 6981 BstYI r'GATC_y Cuts at: 1972 4680 5062 5429 5814 5831 7283 7369 7381 Size: 2708 382 367 385 17 1452 86 12 Cuts at: 7381 7727 1972 Size: 346 1977 Fragments arranged by size: 2708 1977 1452 385 382 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2388 2974 3156 3840 2388 Size: 586 182 684 6280 Fragments arranged by size: 6280 684 586 182 BtgI C'CryG_G Cuts at: 513 1065 2151 513 Size: 552 1086 6094 Fragments arranged by size: 6094 1086 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6993 Fragments arranged by size: 6993 683 56 BtsI GCAGTG_nn' Cuts at: 2548 2876 3209 5248 6042 6062 2548 Size: 328 333 2039 794 20 4218 Fragments arranged by size: 4218 2039 794 333 328 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7732 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7697 DraI TTT'AAA Cuts at: 5036 5036 Size: 7732 DraIII CAC_nnn'GTG Cuts at: 2531 2531 Size: 7732 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6739 809 Size: 507 5423 1802 Fragments arranged by size: 5423 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2751 3057 3273 Size: 54 1425 50 275 18 921 306 216 Cuts at: 3273 3435 4629 5179 6068 7697 8 Size: 162 1194 550 889 1629 43 Fragments arranged by size: 1629 1425 1194 921 889 550 306 275 216 162 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5179 1537 Size: 275 3367 4090 Fragments arranged by size: 4090 3367 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2152 3774 5662 1353 Size: 799 1622 1888 3423 Fragments arranged by size: 3423 1888 1622 799 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6319 6693 6839 387 Size: 1064 138 4730 374 146 1280 Fragments arranged by size: 4730 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2803 2970 3997 4594 2803 Size: 167 1027 597 5941 Fragments arranged by size: 5941 1027 597 167 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2189 2364 2579 2789 2861 3086 3101 Size: 393 557 175 215 210 72 225 15 Cuts at: 3101 3386 3702 3732 3798 4535 4610 4661 4673 Size: 285 316 30 66 737 75 51 12 Cuts at: 4673 4811 5795 6392 7179 7447 1239 Size: 138 984 597 787 268 1524 Fragments arranged by size: 1524 984 787 737 597 557 393 316 285 268 225 215 210 175 138 75 72 66 51 30 15 12 EcoNI CCTnn'n_nnAGG Cuts at: 2690 2924 3155 4829 2690 Size: 234 231 1674 5593 Fragments arranged by size: 5593 1674 234 231 EcoO109I rG'GnC_Cy Cuts at: 2069 2070 2400 3262 3452 3680 3807 3814 3815 Size: 1 330 862 190 228 127 7 1 Cuts at: 3815 3878 3890 4462 4636 2069 Size: 63 12 572 174 5165 Fragments arranged by size: 5165 862 572 330 228 190 174 127 63 12 7 1 1 EcoRI G'AATT_C Cuts at: 5041 5419 5041 Size: 378 7354 FspI TGC'GCA Cuts at: 3410 4577 6238 3410 Size: 1167 1661 4904 Fragments arranged by size: 4904 1661 1167 HaeII r_GCGC'y Cuts at: 1678 2387 2635 3130 4061 6879 1678 Size: 709 248 495 931 2818 2531 Fragments arranged by size: 2818 2531 931 709 495 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2048 2080 3472 3504 6373 Size: 32 135 32 476 32 1392 32 2869 Cuts at: 6373 6405 6447 6479 1373 Size: 32 42 32 2626 Fragments arranged by size: 2869 2626 1392 476 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4860 5076 5322 669 Size: 988 281 2922 216 246 3079 Fragments arranged by size: 3079 2922 988 281 246 216 HindIII A'AGCT_T Cuts at: 748 3542 748 Size: 2794 4938 HpaI GTT'AAC Cuts at: 5322 5322 Size: 7732 KpnI G_GTAC'C Cuts at: 5057 5057 Size: 7732 MfeI C'AATT_G Cuts at: 5331 5331 Size: 7732 MluI A'CGCG_T Cuts at: 7608 7608 Size: 7732 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2241 6846 7030 1305 Size: 936 4605 184 2007 Fragments arranged by size: 4605 2007 936 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2753 3059 3275 10 Size: 54 1425 343 921 306 216 4467 Fragments arranged by size: 4467 1425 921 343 306 216 54 MslI CAynn'nnrTG Cuts at: 518 1166 3749 5690 6049 6208 518 Size: 648 2583 1941 359 159 2042 Fragments arranged by size: 2583 2042 1941 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2323 2482 2594 2761 3364 4072 4097 4495 Size: 717 159 112 167 603 708 25 398 Cuts at: 4495 4627 4651 5148 5825 6973 7218 1606 Size: 132 24 497 677 1148 245 2120 Fragments arranged by size: 2120 1148 717 708 677 603 497 398 245 167 159 132 112 25 24 NaeI GCC'GGC Cuts at: 2053 2505 2053 Size: 452 7280 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7180 NdeI CA'TA_TG Cuts at: 387 387 Size: 7732 NgoMIV G'CCGG_C Cuts at: 2051 2503 2051 Size: 452 7280 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7732 NotI GC'GGCC_GC Cuts at: 5179 5179 Size: 7732 NspI r_CATG'y Cuts at: 1132 1229 2421 3010 3193 4327 5090 6635 1132 Size: 97 1192 589 183 1134 763 1545 2229 Fragments arranged by size: 2229 1545 1192 1134 763 589 183 97 PasI CC'CwG_GG Cuts at: 1348 3656 3803 3811 1348 Size: 2308 147 8 5269 Fragments arranged by size: 5269 2308 147 8 PciI A'CATG_T Cuts at: 2417 6631 2417 Size: 4214 3518 PflMI CCAn_nnn'nTGG Cuts at: 1108 3055 3725 4588 1108 Size: 1947 670 863 4252 Fragments arranged by size: 4252 1947 863 670 PfoI T'CCnGG_A Cuts at: 2805 4143 4827 2805 Size: 1338 684 5710 Fragments arranged by size: 5710 1338 684 PmeI GTTT'AAAC Cuts at: 5036 5036 Size: 7732 PmlI CAC'GTG Cuts at: 4660 4660 Size: 7732 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5801 5833 7314 7346 5801 Size: 32 1481 32 6187 Fragments arranged by size: 6187 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2400 3878 3890 4462 4636 2400 Size: 1478 12 572 174 5496 Fragments arranged by size: 5496 1478 572 174 12 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7732 PsiI TTA'TAA Cuts at: 5302 5302 Size: 7732 PspOMI G'GGCC_C Cuts at: 2069 2316 3452 3579 3807 3814 2069 Size: 247 1136 127 228 7 5987 Fragments arranged by size: 5987 1136 247 228 127 7 PstI C_TGCA'G Cuts at: 830 2745 2766 3093 3198 3336 4245 5084 830 Size: 1915 21 327 105 138 909 839 3478 Fragments arranged by size: 3478 1915 909 839 327 138 105 21 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7732 PvuII CAG'CTG Cuts at: 2323 2482 2594 2761 3364 4495 4627 4651 2323 Size: 159 112 167 603 1131 132 24 5404 Fragments arranged by size: 5404 1131 603 167 159 132 112 24 SacI G_AGCT'C Cuts at: 2805 2972 3999 4596 2805 Size: 167 1027 597 5941 Fragments arranged by size: 5941 1027 597 167 SalI G'TCGA_C Cuts at: 1936 5074 1936 Size: 3138 4594 SanDI GG'GwC_CC Cuts at: 2400 2400 Size: 7732 SbfI CC_TGCA'GG Cuts at: 5084 5084 Size: 7732 ScaI AGT'ACT Cuts at: 2795 5980 7692 2795 Size: 3185 1712 2835 Fragments arranged by size: 3185 2835 1712 SfcI C'TryA_G Cuts at: 826 1045 2177 2627 2741 2762 3089 3194 3332 Size: 219 1132 450 114 21 327 105 138 Cuts at: 3332 3900 4241 4349 4611 5080 6215 6896 7087 Size: 568 341 108 262 469 1135 681 191 Cuts at: 7087 826 Size: 1471 Fragments arranged by size: 1471 1135 1132 681 568 469 450 341 327 262 219 191 138 114 108 105 21 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7732 SgrAI Cr'CCGG_yG Cuts at: 1700 2051 1700 Size: 351 7381 SmaI CCC'GGG Cuts at: 1872 3827 5059 1872 Size: 1955 1232 4545 Fragments arranged by size: 4545 1955 1232 SmlI C'TyrA_G Cuts at: 820 1017 1943 3131 3916 3977 3998 4472 4736 Size: 197 926 1188 785 61 21 474 264 Cuts at: 4736 5836 6737 6999 820 Size: 1100 901 262 1553 Fragments arranged by size: 1553 1188 1100 926 901 785 474 264 262 197 61 21 SnaBI TAC'GTA Cuts at: 493 5029 5523 493 Size: 4536 494 2702 Fragments arranged by size: 4536 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7732 SphI G_CATG'C Cuts at: 1132 3010 3193 5090 1132 Size: 1878 183 1897 3774 Fragments arranged by size: 3774 1897 1878 183 SspI AAT'ATT Cuts at: 5 52 5656 5 Size: 47 5604 2081 Fragments arranged by size: 5604 2081 47 StuI AGG'CCT Cuts at: 1517 2615 2930 1517 Size: 1098 315 6319 Fragments arranged by size: 6319 1098 315 StyI C'CwwG_G Cuts at: 513 1065 2508 2586 2832 3886 3939 4062 4465 Size: 552 1443 78 246 1054 53 123 403 Cuts at: 4465 5171 513 Size: 706 3074 Fragments arranged by size: 3074 1443 1054 706 552 403 246 123 78 53 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2519 3639 4244 5759 5776 5929 6114 Size: 1255 939 1120 605 1515 17 153 185 Cuts at: 6114 6559 325 Size: 445 1498 Fragments arranged by size: 1515 1498 1255 1120 939 605 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2661 2793 3553 Size: 274 80 33 51 1443 684 132 760 Cuts at: 3553 4047 4845 5978 7690 96 Size: 494 798 1133 1712 138 Fragments arranged by size: 1712 1443 1133 798 760 684 494 274 138 132 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2855 2887 7692 7724 2855 Size: 32 4805 32 2863 Fragments arranged by size: 4805 2863 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7732 XbaI T'CTAG_A Cuts at: 5068 5068 Size: 7732 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2158 3067 3613 2158 Size: 909 546 6277 Fragments arranged by size: 6277 909 546 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7732 XmaI C'CCGG_G Cuts at: 1870 3825 5057 1870 Size: 1955 1232 4545 Fragments arranged by size: 4545 1955 1232 XmnI GAAnn'nnTTC Cuts at: 2343 5861 2343 Size: 3518 4214 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5540 276 Size: 53 83 186 936 4006 2468 Fragments arranged by size: 4006 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BbeI BbvCI BfrBI BsiWI BstZ17I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI