(Circular) (Six-base) MAPSORT of: pFN21ASDA0372.seq Check: 5374 from: 1 to: 9421 pFN21ASDA0372 9421 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7231 278 Size: 53 83 186 936 5695 2468 Fragments arranged by size: 5695 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4101 4343 6301 6764 1937 Size: 2164 242 1958 463 4594 Fragments arranged by size: 4594 2164 1958 463 242 Acc65I G'GTAC_C Cuts at: 6594 6742 6594 Size: 148 9273 AclI AA'CG_TT Cuts at: 1563 7548 7921 1563 Size: 5985 373 3063 Fragments arranged by size: 5985 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3041 3111 3303 3491 4756 4824 5489 6072 Size: 1409 70 192 188 1265 68 665 583 Cuts at: 6072 7484 8868 9136 1632 Size: 1412 1384 268 1917 Fragments arranged by size: 1917 1412 1409 1384 1265 665 583 268 192 188 70 68 AflII C'TTAA_G Cuts at: 820 1017 3847 4430 820 Size: 197 2830 583 5811 Fragments arranged by size: 5811 2830 583 197 AflIII A'CryG_T Cuts at: 1203 6244 8320 9297 1203 Size: 5041 2076 977 1327 Fragments arranged by size: 5041 2076 1327 977 AgeI A'CCGG_T Cuts at: 8252 8252 Size: 9421 AhdI GACnn_n'nnGTC Cuts at: 6528 8150 6528 Size: 1622 7799 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 9421 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4859 1184 Size: 3675 5746 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3174 3205 3206 3237 3896 3928 3174 Size: 31 1 31 659 32 8667 Fragments arranged by size: 8667 659 32 31 31 1 AlwNI CAG_nnn'CTG Cuts at: 2521 3776 6173 6238 7164 8736 2521 Size: 1255 2397 65 926 1572 3206 Fragments arranged by size: 3206 2397 1572 1255 926 65 ApaLI G'TGCA_C Cuts at: 7478 8634 7478 Size: 1156 8265 ApoI r'AATT_y Cuts at: 1493 2663 4947 5966 6076 6730 6956 7108 9217 Size: 1170 2284 1019 110 654 226 152 2109 Cuts at: 9217 1493 Size: 1697 Fragments arranged by size: 2284 2109 1697 1170 1019 654 226 152 110 AseI AT'TA_AT Cuts at: 160 1032 4374 4599 7975 160 Size: 872 3342 225 3376 1606 Fragments arranged by size: 3376 3342 1606 872 225 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9421 AvaI C'yCGr_G Cuts at: 1870 1943 5622 6746 1870 Size: 73 3679 1124 4545 Fragments arranged by size: 4545 3679 1124 73 AvrII C'CTAG_G Cuts at: 3955 3955 Size: 9421 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3363 3396 6233 6266 6578 6611 3363 Size: 33 2837 33 312 33 6173 Fragments arranged by size: 6173 2837 312 33 33 33 BamHI G'GATC_C Cuts at: 6751 7118 6751 Size: 367 9054 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5505 6594 6742 8197 618 Size: 325 217 627 3718 1089 148 1455 1842 Fragments arranged by size: 3718 1842 1455 1089 627 325 217 148 BanII G_rGCy'C Cuts at: 1391 3361 3562 4296 6740 1391 Size: 1970 201 734 2444 4072 Fragments arranged by size: 4072 2444 1970 734 201 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2048 3071 3258 5070 5184 928 Size: 971 149 1023 187 1812 114 5165 Fragments arranged by size: 5165 1812 1023 971 187 149 114 BbvCI CC'TCA_GC Cuts at: 4737 4737 Size: 9421 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7612 7646 1903 Size: 34 5675 34 3678 Fragments arranged by size: 5675 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2745 3481 6573 7313 8523 2745 Size: 736 3092 740 1210 3643 Fragments arranged by size: 3643 3092 1210 740 736 BclI T'GATC_A Cuts at: 1548 2191 3151 3993 6094 6403 1548 Size: 643 960 842 2101 309 4566 Fragments arranged by size: 4566 2101 960 842 643 309 BfrBI ATG'CAT Cuts at: 2980 4798 5027 2980 Size: 1818 229 7374 Fragments arranged by size: 7374 1818 229 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2850 3299 8032 Size: 107 122 71 631 605 1178 449 4733 Cuts at: 8032 136 Size: 1525 Fragments arranged by size: 4733 1525 1178 631 605 449 122 107 71 BglII A'GATC_T Cuts at: 9416 9416 Size: 9421 BlpI GC'TnA_GC Cuts at: 4560 6838 4560 Size: 2278 7143 Bme1580I G_kGCm'C Cuts at: 1790 7482 8638 1790 Size: 5692 1156 2573 Fragments arranged by size: 5692 2573 1156 BmgBI CAC'GTC Cuts at: 1318 6643 1318 Size: 5325 4096 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2600 7152 8110 Size: 416 53 83 399 24 1183 4552 958 Cuts at: 8110 442 Size: 1753 Fragments arranged by size: 4552 1753 1183 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 4181 1056 Size: 3125 6296 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3633 4463 4877 5805 6167 8081 1239 Size: 2394 830 414 928 362 1914 2579 Fragments arranged by size: 2579 2394 1914 928 830 414 362 Bpu10I CC'TnA_GC Cuts at: 2298 2854 3725 4737 2298 Size: 556 871 1012 6982 Fragments arranged by size: 6982 1012 871 556 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3317 3383 3750 6328 7546 8411 8709 3317 Size: 66 367 2578 1218 865 298 4029 Fragments arranged by size: 4029 2578 1218 865 367 298 66 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5616 6009 8084 882 Size: 617 4117 393 2075 2219 Fragments arranged by size: 4117 2219 2075 617 393 BsaAI yAC'GTr Cuts at: 493 1204 6718 7212 493 Size: 711 5514 494 2702 Fragments arranged by size: 5514 2702 711 494 BsaBI GATnn'nnATC Cuts at: 2714 9415 2714 Size: 6701 2720 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6529 7228 7610 8245 Size: 53 83 186 936 4996 699 382 635 Cuts at: 8245 275 Size: 1451 Fragments arranged by size: 4996 1451 936 699 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 7854 8252 8526 8673 9084 7854 Size: 398 274 147 411 8191 Fragments arranged by size: 8191 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 5652 5682 1178 Size: 30 165 30 4249 30 4917 Fragments arranged by size: 4917 4249 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3870 4286 1186 Size: 2684 416 6321 Fragments arranged by size: 6321 2684 416 BseYI C'CCAG_C Cuts at: 4181 4963 5843 8624 9120 4181 Size: 782 880 2781 496 4482 Fragments arranged by size: 4482 2781 880 782 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2493 2920 4819 5769 6052 6570 1163 Size: 573 757 427 1899 950 283 518 4014 Fragments arranged by size: 4014 1899 950 757 573 518 427 283 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6871 7632 8660 1540 Size: 275 186 4870 761 1028 2301 Fragments arranged by size: 4870 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3361 3562 4141 4296 5413 7482 7567 8638 3361 Size: 201 579 155 1117 2069 85 1071 4144 Fragments arranged by size: 4144 2069 1117 1071 579 201 155 85 BsiWI C'GTAC_G Cuts at: 3143 3143 Size: 9421 BsmI GAATG_Cn' Cuts at: 2590 3760 4049 4513 4906 6932 7025 2590 Size: 1170 289 464 393 2026 93 4986 Fragments arranged by size: 4986 2026 1170 464 393 289 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3528 3528 Size: 9421 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3361 3562 4141 4296 5413 6740 7482 Size: 399 1571 201 579 155 1117 1327 742 Cuts at: 7482 7567 8638 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1571 1327 1117 1071 742 579 399 201 155 85 BspEI T'CCGG_A Cuts at: 9084 9084 Size: 9421 BspHI T'CATG_A Cuts at: 2671 4663 5208 7310 9040 2671 Size: 1992 545 2102 1730 3052 Fragments arranged by size: 3052 2102 1992 1730 545 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 6776 844 Size: 5932 3489 BsrBI CCG'CTC Cuts at: 1394 1823 7308 1394 Size: 429 5485 3507 Fragments arranged by size: 5485 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4841 5348 5430 7916 8090 65 Size: 1183 3593 507 82 2486 174 1396 Fragments arranged by size: 3593 2486 1396 1183 507 174 82 BsrFI r'CCGG_y Cuts at: 1700 1810 8065 8252 1700 Size: 110 6255 187 2869 Fragments arranged by size: 6255 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2728 5724 96 Size: 2632 2996 3793 Fragments arranged by size: 3793 2996 2632 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9421 BssSI C'ACGA_G Cuts at: 3561 7481 8493 3561 Size: 3920 1012 4489 Fragments arranged by size: 4489 3920 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 5024 5639 6368 6584 794 Size: 4230 615 729 216 3631 Fragments arranged by size: 4230 3631 729 615 216 BstBI TT'CG_AA Cuts at: 2007 8225 2007 Size: 6218 3203 BstEII G'GTnAC_C Cuts at: 1184 5594 1184 Size: 4410 5011 BstYI r'GATC_y Cuts at: 1972 2335 2392 4765 6751 7118 7503 7520 8972 Size: 363 57 2373 1986 367 385 17 1452 Cuts at: 8972 9058 9070 9416 1972 Size: 86 12 346 1977 Fragments arranged by size: 2373 1986 1977 1452 385 367 363 346 86 57 17 12 BtgI C'CryG_G Cuts at: 513 1065 4761 513 Size: 552 3696 5173 Fragments arranged by size: 5173 3696 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8682 Fragments arranged by size: 8682 683 56 BtsI GCAGTG_nn' Cuts at: 3777 4479 5551 5727 6239 6937 7731 7751 3777 Size: 702 1072 176 512 698 794 20 5447 Fragments arranged by size: 5447 1072 794 702 698 512 176 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9421 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9386 DraI TTT'AAA Cuts at: 4029 5359 6081 6725 4029 Size: 1330 722 644 6725 Fragments arranged by size: 6725 1330 722 644 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8428 809 Size: 507 7112 1802 Fragments arranged by size: 7112 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 6410 6868 7757 Size: 54 1425 50 275 18 4580 458 889 Cuts at: 7757 9386 8 Size: 1629 43 Fragments arranged by size: 4580 1629 1425 889 458 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6868 1537 Size: 275 5056 4090 Fragments arranged by size: 5056 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2478 3130 3268 3423 4775 7351 1353 Size: 1125 652 138 155 1352 2576 3423 Fragments arranged by size: 3423 2576 1352 1125 652 155 138 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 8008 8382 8528 387 Size: 1064 138 6419 374 146 1280 Fragments arranged by size: 6419 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3359 3560 4294 3359 Size: 201 734 8486 Fragments arranged by size: 8486 734 201 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3041 3111 3303 3491 3633 4463 4756 Size: 393 1409 70 192 188 142 830 293 Cuts at: 4756 4824 4877 5489 5805 6072 6167 7484 8081 Size: 68 53 612 316 267 95 1317 597 Cuts at: 8081 8868 9136 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1409 1317 830 787 612 597 393 316 293 268 267 192 188 142 95 70 68 53 EcoNI CCTnn'n_nnAGG Cuts at: 3724 3724 Size: 9421 EcoRI G'AATT_C Cuts at: 4947 6730 7108 4947 Size: 1783 378 7260 Fragments arranged by size: 7260 1783 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2323 3017 5471 6472 2323 Size: 694 2454 1001 5272 Fragments arranged by size: 5272 2454 1001 694 FspI TGC'GCA Cuts at: 7927 7927 Size: 9421 HaeII r_GCGC'y Cuts at: 1678 8568 1678 Size: 6890 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2055 2087 3107 3139 3245 Size: 32 135 32 483 32 1020 32 106 Cuts at: 3245 3277 3593 3625 6514 6546 8062 8094 8136 Size: 32 316 32 2889 32 1516 32 42 Cuts at: 8136 8168 1373 Size: 32 2626 Fragments arranged by size: 2889 2626 1516 1020 483 316 135 106 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2353 3628 5157 6765 7011 669 Size: 988 281 415 1275 1529 1608 246 3079 Fragments arranged by size: 3079 1608 1529 1275 988 415 281 246 HindIII A'AGCT_T Cuts at: 748 2094 3199 6449 6483 748 Size: 1346 1105 3250 34 3686 Fragments arranged by size: 3686 3250 1346 1105 34 HpaI GTT'AAC Cuts at: 7011 7011 Size: 9421 KpnI G_GTAC'C Cuts at: 6598 6746 6598 Size: 148 9273 MfeI C'AATT_G Cuts at: 2561 3817 6305 7020 2561 Size: 1256 2488 715 4962 Fragments arranged by size: 4962 2488 1256 715 MluI A'CGCG_T Cuts at: 9297 9297 Size: 9421 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4726 6231 6308 8535 8719 1305 Size: 3421 1505 77 2227 184 2007 Fragments arranged by size: 3421 2227 2007 1505 184 77 MscI TGG'CCA Cuts at: 10 64 1489 1832 6412 10 Size: 54 1425 343 4580 3019 Fragments arranged by size: 4580 3019 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2292 5868 6612 7379 7738 7897 518 Size: 648 1126 3576 744 767 359 159 2042 Fragments arranged by size: 3576 2042 1126 767 744 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2174 6837 7514 8662 8907 1606 Size: 568 4663 677 1148 245 2120 Fragments arranged by size: 4663 2120 1148 677 568 245 NcoI C'CATG_G Cuts at: 513 1065 4761 513 Size: 552 3696 5173 Fragments arranged by size: 5173 3696 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 9421 NheI G'CTAG_C Cuts at: 1052 4177 1052 Size: 3125 6296 NotI GC'GGCC_GC Cuts at: 6868 6868 Size: 9421 NsiI A_TGCA'T Cuts at: 2982 4800 5029 2982 Size: 1818 229 7374 Fragments arranged by size: 7374 1818 229 NspI r_CATG'y Cuts at: 1132 1229 4530 6248 6779 8324 1132 Size: 97 3301 1718 531 1545 2229 Fragments arranged by size: 3301 2229 1718 1545 531 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 9421 PciI A'CATG_T Cuts at: 6244 8320 6244 Size: 2076 7345 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 9421 PfoI T'CCnGG_A Cuts at: 2331 6144 2331 Size: 3813 5608 PmeI GTTT'AAAC Cuts at: 6725 6725 Size: 9421 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7490 7522 9003 9035 7490 Size: 32 1481 32 7876 Fragments arranged by size: 7876 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9421 PsiI TTA'TAA Cuts at: 5223 6991 5223 Size: 1768 7653 PstI C_TGCA'G Cuts at: 830 2173 3622 5062 6280 6773 830 Size: 1343 1449 1440 1218 493 3478 Fragments arranged by size: 3478 1449 1440 1343 1218 493 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9421 PvuII CAG'CTG Cuts at: 2174 2174 Size: 9421 SacI G_AGCT'C Cuts at: 3361 3562 4296 3361 Size: 201 734 8486 Fragments arranged by size: 8486 734 201 SalI G'TCGA_C Cuts at: 1936 6763 1936 Size: 4827 4594 SapI GCTCTTCn'nnn_ Cuts at: 2478 2478 Size: 9421 SbfI CC_TGCA'GG Cuts at: 6773 6773 Size: 9421 ScaI AGT'ACT Cuts at: 2631 7669 9381 2631 Size: 5038 1712 2671 Fragments arranged by size: 5038 2671 1712 SfcI C'TryA_G Cuts at: 826 1045 2169 3618 3975 4142 4782 5058 6276 Size: 219 1124 1449 357 167 640 276 1218 Cuts at: 6276 6769 7904 8585 8776 826 Size: 493 1135 681 191 1471 Fragments arranged by size: 1471 1449 1218 1135 1124 681 640 493 357 276 219 191 167 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9421 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9421 SmaI CCC'GGG Cuts at: 1872 6748 1872 Size: 4876 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3296 3362 3765 3847 4430 5622 Size: 197 926 1353 66 403 82 583 1192 Cuts at: 5622 6343 7525 8426 8688 820 Size: 721 1182 901 262 1553 Fragments arranged by size: 1553 1353 1192 1182 926 901 721 583 403 262 197 82 66 SnaBI TAC'GTA Cuts at: 493 6718 7212 493 Size: 6225 494 2702 Fragments arranged by size: 6225 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 9421 SphI G_CATG'C Cuts at: 1132 6779 1132 Size: 5647 3774 SspI AAT'ATT Cuts at: 5 52 4127 7345 5 Size: 47 4075 3218 2081 Fragments arranged by size: 4075 3218 2081 47 StuI AGG'CCT Cuts at: 1517 2696 2844 6443 1517 Size: 1179 148 3599 4495 Fragments arranged by size: 4495 3599 1179 148 StyI C'CwwG_G Cuts at: 513 1065 3955 4303 4366 4761 5536 6259 6860 Size: 552 2890 348 63 395 775 723 601 Cuts at: 6860 513 Size: 3074 Fragments arranged by size: 3074 2890 775 723 601 552 395 348 63 SwaI ATTT'AAAT Cuts at: 5359 5359 Size: 9421 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3413 4028 7448 7465 7618 7803 8248 Size: 1255 1833 615 3420 17 153 185 445 Cuts at: 8248 325 Size: 1498 Fragments arranged by size: 3420 1833 1498 1255 615 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2531 2629 2728 Size: 274 80 33 51 1443 554 98 99 Cuts at: 2728 4881 5724 6175 7667 9379 96 Size: 2153 843 451 1492 1712 138 Fragments arranged by size: 2153 1712 1492 1443 843 554 451 274 138 99 98 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 9381 9413 9381 Size: 32 9389 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9421 XbaI T'CTAG_A Cuts at: 6757 6757 Size: 9421 XcmI CCAnnnn_n'nnnnTGG Cuts at: 6231 6231 Size: 9421 XhoI C'TCGA_G Cuts at: 1943 5622 1943 Size: 3679 5742 XmaI C'CCGG_G Cuts at: 1870 6746 1870 Size: 4876 4545 XmnI GAAnn'nnTTC Cuts at: 2487 2997 3138 3255 3881 4947 5394 5758 7550 Size: 510 141 117 626 1066 447 364 1792 Cuts at: 7550 2487 Size: 4358 Fragments arranged by size: 4358 1792 1066 626 510 447 364 141 117 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7229 276 Size: 53 83 186 936 5695 2468 Fragments arranged by size: 5695 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI BbeI BplI BstXI BstZ17I Bsu36I DraIII EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI