(Circular) (Six-base) MAPSORT of: pFN21ASDA0263.seq Check: 9609 from: 1 to: 6052 pFN21ASDA0263 6052 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2803 2803 Size: 6052 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3862 278 Size: 53 83 186 936 2326 2468 Fragments arranged by size: 2468 2326 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3395 1937 Size: 1458 4594 Acc65I G'GTAC_C Cuts at: 3373 3373 Size: 6052 AclI AA'CG_TT Cuts at: 1563 4179 4552 1563 Size: 2616 373 3063 Fragments arranged by size: 3063 2616 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2717 4115 5499 5767 1632 Size: 1085 1398 1384 268 1917 Fragments arranged by size: 1917 1398 1384 1085 268 AfeI AGC'GCT Cuts at: 2192 2192 Size: 6052 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5855 AflIII A'CryG_T Cuts at: 1203 2727 3288 4951 5928 1203 Size: 1524 561 1663 977 1327 Fragments arranged by size: 1663 1524 1327 977 561 AgeI A'CCGG_T Cuts at: 2391 4883 2391 Size: 2492 3560 AhdI GACnn_n'nnGTC Cuts at: 3079 4781 3079 Size: 1702 4350 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6052 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2156 1184 Size: 972 5080 AlwNI CAG_nnn'CTG Cuts at: 2596 2802 3795 5367 2596 Size: 206 993 1572 3281 Fragments arranged by size: 3281 1572 993 206 ApaLI G'TGCA_C Cuts at: 2663 4109 5265 2663 Size: 1446 1156 3450 Fragments arranged by size: 3450 1446 1156 ApoI r'AATT_y Cuts at: 1493 3361 3587 3739 5848 1493 Size: 1868 226 152 2109 1697 Fragments arranged by size: 2109 1868 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4606 160 Size: 872 3574 1606 Fragments arranged by size: 3574 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6052 AvaI C'yCGr_G Cuts at: 1870 1943 2181 3170 3377 1870 Size: 73 238 989 207 4545 Fragments arranged by size: 4545 989 238 207 73 BamHI G'GATC_C Cuts at: 3382 3749 3382 Size: 367 5685 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3373 4828 618 Size: 325 217 627 1586 1455 1842 Fragments arranged by size: 1842 1586 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2106 2459 2874 3371 1391 Size: 715 353 415 497 4072 Fragments arranged by size: 4072 715 497 415 353 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5081 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4243 4277 1903 Size: 34 2306 34 3678 Fragments arranged by size: 3678 2306 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3944 5154 3944 Size: 1210 4842 BclI T'GATC_A Cuts at: 1548 1548 Size: 6052 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4663 136 Size: 107 122 71 631 605 2991 1525 Fragments arranged by size: 2991 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6047 6047 Size: 6052 BlpI GC'TnA_GC Cuts at: 2597 3469 2597 Size: 872 5180 Bme1580I G_kGCm'C Cuts at: 1790 2667 2708 4113 5269 1790 Size: 877 41 1405 1156 2573 Fragments arranged by size: 2573 1405 1156 877 41 BmgBI CAC'GTC Cuts at: 1318 2936 1318 Size: 1618 4434 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3783 4741 442 Size: 416 53 83 399 24 2366 958 1753 Fragments arranged by size: 2366 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6052 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2100 2120 2590 3001 3008 3320 4712 1239 Size: 861 20 470 411 7 312 1392 2579 Fragments arranged by size: 2579 1392 861 470 411 312 20 7 Bpu10I CC'TnA_GC Cuts at: 2957 2957 Size: 6052 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2462 2560 2672 3080 4177 5042 5340 2462 Size: 98 112 408 1097 865 298 3174 Fragments arranged by size: 3174 1097 865 408 298 112 98 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2076 2438 4715 882 Size: 617 577 362 2277 2219 Fragments arranged by size: 2277 2219 617 577 362 BsaAI yAC'GTr Cuts at: 493 1204 3289 3349 3843 493 Size: 711 2085 60 494 2702 Fragments arranged by size: 2702 2085 711 494 60 BsaBI GATnn'nnATC Cuts at: 6046 6046 Size: 6052 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2793 3128 3859 4241 Size: 53 83 186 936 1260 335 731 382 Cuts at: 4241 4876 275 Size: 635 1451 Fragments arranged by size: 1451 1260 936 731 635 382 335 186 83 53 BsaWI w'CCGG_w Cuts at: 2391 4485 4883 5157 5304 5715 2391 Size: 2094 398 274 147 411 2728 Fragments arranged by size: 2728 2094 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2563 2593 1178 Size: 30 165 30 1160 30 4637 Fragments arranged by size: 4637 1160 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2435 3175 1186 Size: 1249 740 4063 Fragments arranged by size: 4063 1249 740 BseYI C'CCAG_C Cuts at: 2788 3143 3155 5255 5751 2788 Size: 355 12 2100 496 3089 Fragments arranged by size: 3089 2100 496 355 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2372 2474 2897 1163 Size: 573 636 102 423 4318 Fragments arranged by size: 4318 636 573 423 102 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3502 4263 5291 1540 Size: 275 186 1501 761 1028 2301 Fragments arranged by size: 2301 1501 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2459 2667 2777 3117 4113 4198 5269 2459 Size: 208 110 340 996 85 1071 3242 Fragments arranged by size: 3242 1071 996 340 208 110 85 BsmI GAATG_Cn' Cuts at: 2071 3563 3656 2071 Size: 1492 93 4467 Fragments arranged by size: 4467 1492 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3098 3098 Size: 6052 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2106 2459 2667 2708 2777 2874 3117 Size: 399 316 353 208 41 69 97 243 Cuts at: 3117 3371 4113 4198 5269 1391 Size: 254 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 353 316 254 243 208 97 85 69 41 BspEI T'CCGG_A Cuts at: 5715 5715 Size: 6052 BspHI T'CATG_A Cuts at: 3941 5671 3941 Size: 1730 4322 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2803 2937 3407 844 Size: 1959 134 470 3489 Fragments arranged by size: 3489 1959 470 134 BsrBI CCG'CTC Cuts at: 1394 1823 2992 3939 1394 Size: 429 1169 947 3507 Fragments arranged by size: 3507 1169 947 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2899 4547 4721 65 Size: 1183 1651 1648 174 1396 Fragments arranged by size: 1651 1648 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2391 3078 4696 4883 1700 Size: 110 581 687 1618 187 2869 Fragments arranged by size: 2869 1618 687 581 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6052 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6052 BssSI C'ACGA_G Cuts at: 4112 5124 4112 Size: 1012 5040 BstAPI GCAn_nnn'nTGC Cuts at: 794 3051 794 Size: 2257 3795 BstBI TT'CG_AA Cuts at: 2007 4856 2007 Size: 2849 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6052 BstXI CCAn_nnnn'nTGG Cuts at: 2047 2047 Size: 6052 BstYI r'GATC_y Cuts at: 1972 3382 3749 4134 4151 5603 5689 5701 6047 Size: 1410 367 385 17 1452 86 12 346 Cuts at: 6047 1972 Size: 1977 Fragments arranged by size: 1977 1452 1410 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3120 3120 Size: 6052 BtgI C'CryG_G Cuts at: 513 1065 2040 2866 513 Size: 552 975 826 3699 Fragments arranged by size: 3699 975 826 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2736 487 Size: 683 56 1510 3803 Fragments arranged by size: 3803 1510 683 56 BtsI GCAGTG_nn' Cuts at: 2407 2413 3568 4362 4382 2407 Size: 6 1155 794 20 4077 Fragments arranged by size: 4077 1155 794 20 6 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6052 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6017 DraI TTT'AAA Cuts at: 3356 3356 Size: 6052 DraIII CAC_nnn'GTG Cuts at: 2161 2163 2161 Size: 2 6050 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2090 5059 809 Size: 507 774 2969 1802 Fragments arranged by size: 2969 1802 774 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2863 3499 4388 Size: 54 1425 50 275 18 1033 636 889 Cuts at: 4388 6017 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1033 889 636 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3499 1537 Size: 275 1687 4090 Fragments arranged by size: 4090 1687 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3982 1353 Size: 2629 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2139 4639 5013 5159 387 Size: 1064 138 550 2500 374 146 1280 Fragments arranged by size: 2500 1280 1064 550 374 146 138 EcoICRI GAG'CTC Cuts at: 2457 2457 Size: 6052 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2100 2120 2590 2717 3001 3008 3320 Size: 393 468 20 470 127 284 7 312 Cuts at: 3320 4115 4712 5499 5767 1239 Size: 795 597 787 268 1524 Fragments arranged by size: 1524 795 787 597 470 468 393 312 284 268 127 20 7 EcoO109I rG'GnC_Cy Cuts at: 2506 2971 3179 2506 Size: 465 208 5379 Fragments arranged by size: 5379 465 208 EcoRI G'AATT_C Cuts at: 3361 3739 3361 Size: 378 5674 FspI TGC'GCA Cuts at: 4558 4558 Size: 6052 HaeII r_GCGC'y Cuts at: 1678 2194 5199 1678 Size: 516 3005 2531 Fragments arranged by size: 3005 2531 516 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2714 2746 3065 3097 3113 Size: 32 135 32 1142 32 319 32 16 Cuts at: 3113 3145 4693 4725 4767 4799 1373 Size: 32 1548 32 42 32 2626 Fragments arranged by size: 2626 1548 1142 319 135 42 32 32 32 32 32 32 32 16 HincII GTy'rAC Cuts at: 669 1657 1938 3396 3642 669 Size: 988 281 1458 246 3079 Fragments arranged by size: 3079 1458 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6052 HpaI GTT'AAC Cuts at: 3642 3642 Size: 6052 KpnI G_GTAC'C Cuts at: 3377 3377 Size: 6052 MfeI C'AATT_G Cuts at: 3651 3651 Size: 6052 MluI A'CGCG_T Cuts at: 2727 5928 2727 Size: 3201 2851 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2670 5166 5350 1305 Size: 1365 2496 184 2007 Fragments arranged by size: 2496 2007 1365 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4230 Fragments arranged by size: 4230 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2045 2721 2813 4010 4369 4528 518 Size: 648 879 676 92 1197 359 159 2042 Fragments arranged by size: 2042 1197 879 676 648 359 159 92 MspA1I CmG'CkG Cuts at: 1606 2596 2735 2824 3468 4145 5293 5538 1606 Size: 990 139 89 644 677 1148 245 2120 Fragments arranged by size: 2120 1148 990 677 644 245 139 89 NcoI C'CATG_G Cuts at: 513 1065 2040 513 Size: 552 975 4525 Fragments arranged by size: 4525 975 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6052 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6052 NotI GC'GGCC_GC Cuts at: 3499 3499 Size: 6052 NspI r_CATG'y Cuts at: 1132 1229 2608 2812 3410 4955 1132 Size: 97 1379 204 598 1545 2229 Fragments arranged by size: 2229 1545 1379 598 204 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6052 PciI A'CATG_T Cuts at: 4951 4951 Size: 6052 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6052 PfoI T'CCnGG_A Cuts at: 2097 3246 2097 Size: 1149 4903 PmeI GTTT'AAAC Cuts at: 3356 3356 Size: 6052 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4121 4153 5634 5666 4121 Size: 32 1481 32 4507 Fragments arranged by size: 4507 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2506 3179 2506 Size: 673 5379 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6052 PsiI TTA'TAA Cuts at: 3622 3622 Size: 6052 PstI C_TGCA'G Cuts at: 830 2849 3404 830 Size: 2019 555 3478 Fragments arranged by size: 3478 2019 555 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6052 PvuII CAG'CTG Cuts at: 2596 2735 2824 2596 Size: 139 89 5824 Fragments arranged by size: 5824 139 89 SacI G_AGCT'C Cuts at: 2459 2459 Size: 6052 SalI G'TCGA_C Cuts at: 1936 3394 1936 Size: 1458 4594 SanDI GG'GwC_CC Cuts at: 3179 3179 Size: 6052 SbfI CC_TGCA'GG Cuts at: 3404 3404 Size: 6052 ScaI AGT'ACT Cuts at: 2858 4300 6012 2858 Size: 1442 1712 2898 Fragments arranged by size: 2898 1712 1442 SfcI C'TryA_G Cuts at: 826 1045 2845 3400 4535 5216 5407 826 Size: 219 1800 555 1135 681 191 1471 Fragments arranged by size: 1800 1471 1135 681 555 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6052 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6052 SmaI CCC'GGG Cuts at: 1872 2183 3379 1872 Size: 311 1196 4545 Fragments arranged by size: 4545 1196 311 SmlI C'TyrA_G Cuts at: 820 1017 1943 2441 2539 2651 3095 4156 5057 Size: 197 926 498 98 112 444 1061 901 Cuts at: 5057 5319 820 Size: 262 1553 Fragments arranged by size: 1553 1061 926 901 498 444 262 197 112 98 SnaBI TAC'GTA Cuts at: 493 3349 3843 493 Size: 2856 494 2702 Fragments arranged by size: 2856 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6052 SphI G_CATG'C Cuts at: 1132 2608 2812 3410 1132 Size: 1476 204 598 3774 Fragments arranged by size: 3774 1476 598 204 SspI AAT'ATT Cuts at: 5 52 3976 5 Size: 47 3924 2081 Fragments arranged by size: 3924 2081 47 StuI AGG'CCT Cuts at: 1517 2999 1517 Size: 1482 4570 StyI C'CwwG_G Cuts at: 513 1065 2040 2619 3195 3491 513 Size: 552 975 579 576 296 3074 Fragments arranged by size: 3074 975 579 576 552 296 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2640 4079 4096 4249 4434 4879 325 Size: 1255 1060 1439 17 153 185 445 1498 Fragments arranged by size: 1498 1439 1255 1060 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2856 4298 6010 Size: 274 80 33 51 1443 879 1442 1712 Cuts at: 6010 96 Size: 138 Fragments arranged by size: 1712 1443 1442 879 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6012 6044 6012 Size: 32 6020 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6052 XbaI T'CTAG_A Cuts at: 3388 3388 Size: 6052 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2522 2522 Size: 6052 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6052 XmaI C'CCGG_G Cuts at: 1870 2181 3377 1870 Size: 311 1196 4545 Fragments arranged by size: 4545 1196 311 XmnI GAAnn'nnTTC Cuts at: 2225 4181 2225 Size: 1956 4096 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3860 276 Size: 53 83 186 936 2326 2468 Fragments arranged by size: 2468 2326 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI